Sc 9 Biology Review: Answers 1 1. Label the diagram of the cell below. Describe the function of each of the structures. a. Cell membrane – controls entry and exit of molecules into and out of the cell. b. Vacuole or vesicle: storage container c. Smooth endoplasmic reticulum – transports proteins (as well as other things) d. NUCLEOLUS! – site of ribosome production e. Centrioles – involved in cell division and the production of the spindle fibres f. Golgi apparatus- receives proteins from the ER, repackages them and then sends them to where ever they were destined to go. g. Nucleus – stores the DNA and contains the nucleolus h. s Mitochondria – site of cellular respiration (releases the Energy from the glucose molecule for the cell to use) i. Cytoplasm – bathes the organelle j. Ribosomes – site of protein synthesis k. Lysosomes – vesicles filled with digestive enzymes. Used to digest food vesicles and to clean up the cytoplasm from the waste and debris l. Nuclear pores – openings in the nuclear membrane to allow substances into and out of the nucleus Sc 9 Biology Review: Answers 2 m. Rough endoplasmic reticulum (ER covered in Ribosomes) transport the proteins produced by the ribosomes to locations throughout the cell. 2. What does the DNA molecule give instructions for (specifically)? Why do we call this our “blueprints”? The DNA molecule is a recipe book for PROTIENS. We call this our blueprints because once we know how to make proteins, they can work as enzymes. Enzymes control ALL our chemical reactions in our body, and ALL we consist of is a very highly controlled, specific series of chemical reactions. When our enzymes die, so do we, and all our building blocks go back to the earth to be reused for some other organism. 3. What do we call a portion of the DNA molecule that codes for one protein? We call one portion of our DNA that codes for one protein a gene. We have thousands of genes in our entire genome. 4. What are the two main stages in the cell’s cycle? The 2 main stages of the cell cycle are interphase (for growth, repair and DNA replication) and mitosis (for cell division). 5. List the 4 stages of mitosis and briefly describe what happens during each of these stages. Do a sketch to illustrate your answer. Prophase: Nucleus Disappears Chromatids duplicate and form distinct chromosomes Centrioles migrate to opposite poles of the cell Spindle fibres begin to form Metaphase: Chromosomes line up at the equator of the cell Spindle fibres attach to the centromeres of each chromosome Anaphase: Chromatids are separated and pulled to opposite poles of cell by spindle fibres. Telophase: Cell elongates and pinches in at the middle Cytokinesis occurs to divide the rest of the organelles (cell divides into two) 6. What is the difference between chromatin, chromosomes, and chromatids? Chromatin is the indistinguishable mass of DNA molecules that exist in the nucleus during the normal lifetime of the cell. At the time of cell division, the chromatin duplicates and organizes into chromosomes. Chromosomes consist of two chromatids attached together with a centromere. Because of this structure, the chromosomes resemble the letter X when you see them under a microscope. The chromatids are separated during anaphase – one copy going north and the other copy moving south. 7. What is cytokinesis? Cytokinesis is the division of the organelles and other substances in the cytoplasm. Approximately half of the organelles go to each of the new daughter cells, making them practically identical when they separate. 8. What is cancer? What causes cancer? Cancer is uncontrolled cell division. Normally, cells divide in a very coordinated and organized manner. Cancer cells have lost all of this control, and therefore will often cause a lump or mass wherever it occurs. Cancer is caused by MANY things, but all cancers are the result of genetic mutations – the rearrangement of the genetic code inside the nucleus. The mutations usually occur in the genes that code for enzymes that Sc 9 Biology Review: Answers 3 are responsible for cell division control. This code can be altered by radiation, chemicals or just a mistake when the cell is reproducing. 9. Define asexual reproduction. Asexual reproduction produces clones of the original parent; only one parent is involved. 10. List the 5 different types of asexual reproduction mentioned in the text and briefly describe each of the processes. Binary fission: literally dividing the cell into two equal new cells. Budding: a new organism,( identical genetically)grows on the side of the parent until it is large enough to break off and live on its own. Spore formation: A specialized cell containing a complete set of the organism’s genes is formed with a protective coating. The spore may travel far away from the parent before settling and beginning to grow an entire new organism. Fragmentation: part of the parent breaks off and regenerates the rest of the body, while the parent regenerates the part of its body that fell off. Vegetative reproduction: Special stems or roots are grown that begin the growth of a new “section” of the parent plant. The new section will either remain attached to the parent, or become dislodged from it’s connection and continue on it’s own. 11. What are homologous chromosomes? You have 2 complete sets of chromosomes – one set came from Mom and the other set (which is homologous) comes from your Dad. Homologous chromosomes contain the same genes, but they are different versions (Mom’s and Dad’s) of the same gene. For example, chromosomes #5 might both carry the gene for eye colour, but the one from your Mom is a blue eye gene, and the one from your Dad is a brown eye gene. 12. Compare the outcomes of meiosis and mitosis. Mitosis creates 2 genetically identical, diploid cells. Meiosis creates 4 genetically unique, haploid gametes. Mitosis is a form of asexual reproduction, while meiosis produces gametes for the purpose of sexual reproduction. 13. Why do you need the process of meiosis? Why can’t all cells just use mitosis? Meiosis creates haploid cells – human cells are diploid. If we started using mitosis all the time, everytime we reproduced sexually, our chromosome number would double. That would become VERY complicated for the cell. Therefore, for SEX, we need to divide the # of chromosomes in half = MEIOSIS. 14. How is it that you are a mixture of your mother, father and all four of your grandparents? When your parent’s cells begin the process of meiosis(before you were even a fertilized egg), THEIR parent’s homologous chromosomes found each other an mixed up (CROSS-OVERS!). Then meiosis occurred and one of the products was the sperm and the egg that eventually became YOU. THEN the sperm and the egg got together, and the two sets of genes were expressed depending on their dominance relationship with each other. Sometimes your mom’s genes were expressed (which were a mixture of the genes from you maternal grandparents) and sometimes your Dad’s genes were expressed (they were a mixture of your paternal grandparents). Sc 9 Biology Review: Answers 4 15. What types of cells perform meiosis? In humans, where are these cells? Reproductive cells perform meiosis to produce gametes. In humans, the reproductive cells are found in the ovaries or testes. 16. What is the advantage of sexual reproduction? Sexual reproduction mixes up the genes of a species, which provides them with more natural variation in the species. Natural variation allows the species to adapt or evolve to changes in the environment. If we were all exactly the same, a single pathogen might be able to wipe us all out completely. However, natural variation helps ensure that at least SOMEONE will have the natural ability to resist the disease, so the species will survive. 17. What are the advantages of asexual reproduction? Asexual reproduction does not require as much energy as internal fertilization sexual reproduction. You don’t require a mate (which is sometimes hard to find). You can produce large numbers of offspring easily. (DISADVANTAGE: your offspring are all clones) 18. What is a mutation? A change in the sequence or structure of the DNA molecule. It can be negligible, advantageous or deleterious. It usually causes a change in the protein that was being coded for at that location of the change in the DNA. 19. How can chemicals cause mutations? Chemicals can react with the DNA molecule, changing it’s structure and hence changing the sequence of the nucleotides. When you change the DNA code, a mutation occurs. 20. How can radiation cause mutations? Radiation can physically break the DNA molecule and disrupt the chain of nucleotides, causing a mutation. 21. What is the difference between positive, neutral and negative effects of various mutations? Positive mutations actually improve the functioning of the enzyme that is produced after the change. Neutral mutations are changes in the nucleotide sequence that do not affect the function of the enzyme produced by that gene. Negative mutations cause the enzyme to work poorly, or sometimes, not at all. This has a negative affect on the cell and possibly the organism. 22. What is gene therapy? Gene therapy is an experimental procedure that treats genetic disorders by replacing the mutated/dysfunctional genes with a healthy copy of the genes. It is VERY difficult, but researchers are confident that the technique will become successful in the future. 23. Draw a section of the DNA molecule. Label all of the parts, including nucleotides, phosphates, sugars, nitrogenous bases, sense strand, and anti-sense strand. What structures are considered the “backbone” of the molecule? The “rungs” of the ladder? The “backbones” are the sugar and phosphate down the sides, and the “rungs” of the ladder are the nitrogenous bases. Sc 9 Biology Review: Answers 5 24. What part of the DNA molecule carries the code? The sequence of the nucleotides gives the actual code. Specifically, it is the nitrogenous bases within the nucleotides that determines the code. On a structure diagram, these are often called the “rungs of the ladder”. 25. Describe how the code works by comparing it to a sentence. In our genetic code, we only have 4 “letters” to the “alphabet”– the nitrogenous bases adenine (A), guanine (G), Cytosine (C) and Thymine (T). (in the mRNA copy, Uracil (U) replaces thymine). Words are constructed from the sequence of the bases, and are ALWAYS 3 letters (bases) long. These words are called triplets or codons. The words are translated into specific amino acids. There are only 20 amino acids, so there are often 3-4 codons that translate to the same amino acid. “Sentences” begin with the code AUG – which translates into the amino acid methionine. This means “start”. The sentences end with a stop codon – UAA, UAG, or UGA. Sentences usually translate enough amino acids in a chain to form one protein molecule. We call the “sentences” genes. Usually, one gene = one protein. 26. Decode this piece of mRNA: AUG UUU CCA GGC GAC UGA (a code will be provided on the test) met. (start) – phe – pro – gly – asp - stop 27. What does methonine also mean? Start 28. What is therapeutic cloning? What kinds of cells are used in therapeutic cloning? How are these types of cells different that other body cells? Therapeutic cloning uses stem cells to correct health problems. Embryonic Stem cells are undifferentiated cells that retain the ability to develop into ANY type of cell. Most of our normal body cells have lost this ability because their “parent” cells have already differentiated into specialized cell types. These cells can be implanted into tissues that are defective and potentially grow into new, heathly normal cells of the original tissue type. Adult stem cells are found in our bone marrow. They have the ability to develop into any type of blood cell (but ONLY blood cells). They are not as diverse as the embryonic ones. 29. At what stage of development are embryonic stem cells harvested? Embryonic stem cells are formed in the blastula. Sc 9 Biology Review: Answers 6 30. Describe the difference between external and internal fertilization. The difference is just as the titles imply – internal fertilization (what humans do!) involves the sperm fertilizing the egg INSIDE the females’ body. External fertilization (fish do this) involves both parents releasing their gametes outside of their body where they will be mixed for fertilization. This type of fertilization takes less energy to find an appropriate mate, and decreases some of the risks associated with INTERNAL fertilization. 31. Describe the difference between Artificial insemination, In vitro fertilization, gamete intrafallopian transfer and Intracytoplasmic sperm injection. Artificial Insemination: sperm is collected from the male and then inserted into the vagina of the female using a specialized instrument. In Vitro Fertilization: Sperm and eggs are removed from the male and female and mixed in a Petri dish. After the fertilized eggs begin to divide, the embryos are introduced into the uterus of a female in hopes that the embryo will embed in the lining and begin to develop. Gamete intrafallopian transfer – sperm and eggs are mixed in a Petri dish and immediately implanted into the fallopian tube of a female. Ideally, the fertilization event will not actually happen until they are in the fallopian tubes. Intracytoplasmic sperm injection – one sperm is injected into an egg in a Petri dish. Then the zygote (fertilized egg) is inserted into the uterus of a female.