Example Conclusion (Please note that all data and science information is fictional.) A study measuring saliva production in Canis lupis, the domesticated dog, was conducted to examine the relationship between weight and saliva production in five different breeds of dog. The breeds included are Great Dane, St. Bernard, Chihuahua, Toy Poodle, and Golden Retriever. It was predicted that dog breeds with larger body size would produce more saliva, measured in drops of drool per minute, because larger dogs will have larger mouths and salivary glands. It was found that the largest dog breed, the St. Bernard, weighing in at 122 pounds, produced the most saliva, within an average range of 5.6 to 8.6 drops of drool per minute. The data shows a significant difference in the largest breed producing the most saliva. In descending order by body weight, it was determined that the Great Dane and Golden Retriever were grouped together as the next highest saliva producers with averages between 2 and 4.1 drops of drool per minute. The averages ranges overlapped for these two breeds by 0.1 drops of drool per minute, so one cannot differentiate between the two. Both the Great Dane and the Golden Retriever weigh less that the St. Bernard, at 108 and 78 pounds respectively. Both breeds also produce less saliva, as is shown in Graph 1. The pattern continues through the remaining smaller breeds of dog, although the average ranges of saliva production also overlap for the Chihuahua and Toy Poodle. The data supports the hypothesis that the larger the dog breed the greater amount of saliva that is produced. Ideally, each breed would have an average saliva production range that was statistically significant, but the researcher feels that the data supports the relationship between body size and saliva production at least partially. In 2001, a study conducted by Sarah Drewl found that dogs tend to drool in excessive amounts when presented with a treat in full-view. The dog breed used was a larger dog, a Black Lab, and the average amount of saliva produced in drops per minute was found to be 2.5-3.1, which is similar to the Golden Retriever data in this experiment (Drewl). This data can be compared because Golden Retrievers and Black Labs are similar body weights. Additionally, a classmate found that larger dogs excrete more urine, which supports the idea that larger dogs have more saliva (Janiszewski). A primary reason for increased drool and urine production in larger dogs can be explained by the salivary gland placement in animals. Salivary glands are found in the lower portions of the jaw. Just as teeth are larger in larger animals, salivary glands are also larger in animals with higher body weights (Glandular). Salivary glands produce saliva to assist the body with digestive processes. When a dog views treats, the brain tells the digestive system to begin secreting enzymes that will assist the body with mechanical digestion (Yeargin). In particular, the frontal lobe of the brain sends a signal through the nervous system to begin this process. The eyes start the signal and it carries though to the salivary glands (McGolrick). The salivary glands produce a mucous-like substance called saliva that coats the esophagus and helps food travel to the stomach (Pearson). One can conclude that a larger dog not only has larger salivary glands, but also a larger esophagus, and therefore requires more saliva to help food slide down the esophagus. The large average ranges shown by the error bars in graph 1 are most likely due to human error and poor planning. When the procedure was written, there was no plan for what the dog should do as the saliva was measured. Through the ten trials there was 1 variation in the dog’s position. These positions caused some saliva to fall onto the floor or to smear on the arm or face of the researcher. Apart from this process being unpleasant for the person measuring, there was a great deal of uncalculated saliva left on clothing or the floor. Also, the dogs were of varying ages. In the future it is important that the procedure state whether dogs should be sitting or standing. Also, it is important that the saliva collection be as quick as possible so that the drops of drool do not fall to the floor. Ten trials is a small sample size, so if possible it would be ideal to test at least fifty of each dog breed. If further studies were done it would be useful to test this theory in other mammal species. Works Cited Remember that you need 14 authored sources. I’m making this up so I’m adding this only to show the concept of parenthetical citation. Note the color matching of the source to the citation. You don’t have to color code, but it is an option to help you organize your citations. Drewl, S. P. Black Labs and Oral Hygiene. New York: Carnegie Publishing, 1999. Print. Glandular, I., and P. Rocks. “Mammal Digestive Systems.” National Geographic. Dec. 2006: 33-56. Print. Janiszewski, R. “Urine Trouble: A Study of Housebreaking Dogs” Pet Owner Digest. Mar. 2001: 64-72. Print. McGolrick, M. M. The Nervous System: An Owner’s Manual. San Francisco: McDougal, Bagel, and Co. Inc., 1998. Print. Pearson, D. “Don’t Block the Tube: A Study on the Esophageal System.” Health Weekly. June 2004: 12-18. Web. 15 May 2009. Yeargin, A.D. The Stomach. London: Carter Inc., 1997. Print. 2