Chemical Analysis Group Lifecycle
User Information Plan
User Information Plan
Document Information:
Filename
Current Owner
Product Identifier
Project Identifier
Revision Log:
Date
August 23, 2006
USERINFO.DOC
Deborah Perkins
Reason For Update
Initial Revision
Localization: Chinese, contact Xue-fei Xu
Overview
This is a lecture and laboratory course requiring a TOF equipped with an electrospray
source. Extra computers are provided so that students may perform more
individualized work on DataAnalysis only laboratories.
R1874A
General Course Information
Course Name
R1874A - Agilent 6210 LC/MS TOF Techniques and Operation
Course Price
R1874A $3400.00
Duration
4 days
Description
This course is designed for those who want to enhance their skills in the use of the Agilent 6210 LC/MS TOF including
tuning, acquisition, optimization, and data analysis.
Template Revision T.04
Date Printed: 2/16/2016
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User Information Plan
Agenda:
Instrument and Software Overview
Mass Accuracy and Its Uses
TOF Theory
Sample Introduction
Tuning and Optimization
Tuning Laboratory
Acquisition
Acquisition Laboratory
Worklists and Batches
Worklists and Batches Laboratory
Analyst QS for Qualitative Analysis
Qualitative Laboratory
Analyst QS for Quantitation
Quantitation Laboratory
MassHunter Processing Software
MassHunter Laboratory
Bioconfirm Software
Bioconfirm Laboratory
Data Browser
Data Browser Laboratory* optional
Easy Access* optional
Instrument Maintenance
Instrument Maintenance Demonstration
Student Profile:
This course is designed for an analyst who has responsibility for operation of the Agilent 6210 LC/MS TOF for
routine and non-routine samples. A fundamental understanding of liquid chromatography is expected and mass
spectrometry is expected.
Prerequisites:
Techniques of HPLC, H1186A, Practical High Performance Liquid Chromatography, H5930A, or equivalent
experience.as well as understanding of the basic concepts in mass spectrometry.
Equipment
At least 1 Agilent 6210 TOF equipped with an electrospray source, an Agilent 1100 or 1200 HPLC (binary
pump) MassHunter software, Analyst software, Bioconfirm, Data Browser, and Easy Access. Laptop computers
equipped MassHunter software, Analyst software, Bioconfirm, Data Browser, and Easy Access
Template Revision T.04
Date Printed: 2/16/2016
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Printed Copies are Uncontrolled
See Project Plan for location of Controlled Documentation
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Chemical Analysis Group Lifecycle
User Information Plan
Files
R1874Astudent.doc – lecture manual in Word format.
R1874Astudent.pdf – lecture manual in pdf format for printing.
R1874Alaboratory.doc – laboratory manual in Word format.
R18874Alaboratory.pdf – laboratory manual in pdf format for printing.
Datafiles.exe – contains data files and methods needed to execute the course.
All slides for lectures are .ppt files, for instance,
01_instrumentationandSoftwareoverview.ppt.
Execute the corresponding laboratories after each lecture. For instance, execute the tuning
lab after the tuning lecture is delivered.
Course Preparations
Instrumentation Guidelines

A 6210 TOF instrument with workstation is required. An electrospray source and
Agilent 1100 or 1200 HPLC with vacuum degasser, autosampler- high performance,
binary pump, UV detector.

Please have consumables on hand for the TOF and HPLC such as capillaries, ferrules,
etc.

For required samples – see below.

Each student should have a laptop computer with offline software noted above..

This course uses A.02 TOF software.

Load course data files and methods into appropriate folders. Do not delete Demo
files. See below.

Microsoft Excel

Binary Pump, High Performance Autosampler
Template Revision T.04
Date Printed: 2/16/2016
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Other Requirements - Lectures

High resolution LCD Projector capable of handling 1280 x 1024 resolution.

Printed student lecture and lab manual per student.

White board and markers for instructor.

Instructor requires laptop for lectures.
Other Requirements – Laboratories
Consumables:
 Reference and tuning solution appropriate for the installed source.
Suggest ESI source.

A Zorbax Eclipse XDB-C18 column, 4.5 x 50 mm id, Agilent p/n 935967-902

Electrospray LC Demo Sample, Agilent p/n 59987-20033

Solvent A: 0.1 % Formic Acid in Water (HPLC Grade)

Solvent B: 0.1% Formic Acid in Acetonitrile (HPLC Grade)

Sample Vials and Caps for an Agilent 1200 LC Autosampler

The following project files should be loaded into C:\PE Sciex Data\Projects
o Caff
o Test_QS

The following data files to be loaded in C:\Program Files\Agilent\TOF
Software\Data
o Myoglobin.wiff
o BSA_02.wiff
o BR01_01.wiff
o ProTrpMix.wiff
o AC-2-DMSO3b.wiff
o TSA02.wiff
o Cytochrome.wiff
Template Revision T.04
Date Printed: 2/16/2016
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
The following data files to be loaded in C:\Program Files\Agilent\Data
Browser\Sample Data
o 38drugs.aev
o testLC2.aev

The following files to be loaded in C:\Program Files\Agilent\TOF
Software\Worklists
o Reports_tests.wkl

The following data files to be loaded in C:\PE Sciex Data\Projects
o Reports_test

The following data files should be loaded in C:\Program Files\Agilent\TOF
Software\Protein Sequences
o BSA.psq
Note: Make certain that the attributes are not read-only.
Template Revision T.04
Date Printed: 2/16/2016
Agilent Technologies Confidential
Printed Copies are Uncontrolled
See Project Plan for location of Controlled Documentation
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