Dr. Bachman
BIO 212 Spring 2007
Genetics Laboratory
Tentative Schedule
Week Dates Laboratory
Assignments Due
2
1/23
3
1/30
DNA models/Sickle cell
electrophoresis
Intro to Drosophila
4
2/6
Mendelian genetics
Drosophila practical
DNA worksheet
Worksheet #1
5
2/13
Mitosis and meiosis
Mendelian genetics worksheets
6
2/20
Drosophila linkage map
Mitosis and meiosis quiz
7
2/27
Spring Break I--no lab
8
3/6
Drosophila chromatography
9
3/13
Transformation and transduction I
10
3/20
Transformation and transduction II
11
3/27
Lac operon
12
4/3
Spring Break II--no lab
13
4/10
PCR I
14
4/17
PCR II
15
4/24
Bioinformatics
16
5/1
DNA testing
Bioinformatics tutorial
5/8
DNA testing discussion
DNA testing worksheet
Drosophila protocol/
F1 Results
Chromatography worksheet
Transformation/transduction
worksheet (due F)
F2 Results/Linkage map
Lac operon worksheet
Lab practical: micropipets
PCR quiz
PCR worksheet
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Lab objectives:
1. To understand genetic principles through experiments and observations in the
laboratory.
2. To gain experience with techniques of modern genetic research.
Lab preparation and participation:
The labs meet Tues. from 10-11:50 am in room 205 Physical Science. There is no
laboratory manual for this course. Handouts will be passed out in lecture for each week's
lab and will also be available on the course web page. You should read the handouts
carefully before coming to class. Please make an effort to arrive for the start of lab, when
instructions are given. Lab assignments (worksheets, etc.) are described in the lab
handouts and will be clarified in class. You will be expected to attend and carry out every
lab, although there is some flexibility; you may be able to make up the lab at a lab makeup time (typically Friday afternoon). For some labs, especially the Drosophila linkage
lab, you will also need to come in at other times.
Late Assignments:
Most assignments are due the week after the scheduled lab as indicated on the lab
syllabus, unless a later due date is given by the instructor. Late assignments will receive a
10% penalty.
Grading:
Lab practicals (2)
Worksheets and data analysis (7)
Quizzes (2)
Drosophila linkage lab assignments
(3)
Bioinformatics tutorial
Total points
10 pts Drosophila practical
10 pts micropipets
20 pts (DNA worksheet)
20 pts (Mendelian Genetics worksheets--4)
20 pts (Chromatography worksheet)
20 pts (Transformation/Transduction
worksheet)
20 pts (Lac operon worksheet)
10 pts (PCR worksheet)
20 pts (DNA testing worksheet)
20 pts (Mitosis/Meiosis)
10 pts (PCR)
20 pts protocol/F1 results
20 pts F2 results/linkage map
10 pts Drosophila lab work
10 pts.
240 pts.
The lab total will comprise about 40% of the course grade.
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