Content of Lectures Given in Pharmaceutical Microbiology II (PHT 313):
Course topics
Gram-positive cocci:
Number of
lectures
4
Content o Lectures
Gram-negative cocci:
2
Non-spore forming
Gram-positive rods
2
Streptocococcaceae: streptococci & enterococci
Neisseria gonorrhoeae, N. meningitidis &
Baranhemalla.
Corynebacterium & Listeria.
Aerobic spore forming
Gram-positive rods
1
Bacillus anthracis and Bacillus cereus
Anaerobic spore
forming Grampositive rods
Gram-negative rods:
Enterobacteriaceae
Gram-negative rods:
Enterobacteriaceae
Gram-negative rods:
Pseudomonadaceae
Gram-negative rods:
Vibrionaceaeaceae
Gram-negative rods:
Parvobacteria
Gram-negative rods:
Other curved
organisms
Gram-negative rods:
zoonotic organisms
Anaerobic Gram-
1
Clostridium tetani, Clostridium botulinum,
Clostridium perfringenes, Clostridium difficile
2
E. coli, Klebsiella, Citrobacter , Enterobacter"
2
Salmonella, Shigella, Proteus, Yersinia" "
1
Pseudomonas aeruginosa
1
Vibrio cholorae, Vibrio parahaemolyticus
2
Haemophilus, Brucella, Bordetella.
1
Helicobacter & Campylobacter
1
Pasteurella, Francisella.
1
Bacterioids fragilis, and Legionella
Microcococcaceae: staphylococci & micrococci
negative rods
Acid fast bacteria
branched bacteria
Cell wall deficient
organisms
Spirochates
Obligate intracellular
bacteria
Medically important
fungi
1
1
1
Mycobacterium tuberculosis, Mycobacterium
bovis, Mycobacterium leprae & Nocardia
Actinomyces
Chalamydiae & Mycoplasma
1
1
Treponema, Borrelia, Leptospira
Rickettsia, coxiella, Ehrlichia
2
Candida albicans & Cryptococcus neoformans
Laboratory Exercises for Pharmaceutical Microbiology II (PHT 313): (3hours/Lab):
Week
Topic
Lab description
1
Staphylococci
Use of catalase test to differentiate between staphylococci and
streptococci. Laboratory diagnosis of staphylococci by gram stain and
biochemical tests as coagulase, DNase, Mannitol fermentation on
Mannitol salt agar and novobiocin sensitivity tests to differentiate
between S. aureus, S. epidermidis and S. saprophyticus using
2
Streptococci
Use of blood agar to differentiate between , ,  haemolytic
streptococci. Use of bacitracin to identify Strepococcus pyogenes. Use
of CAMP test to identify Streptococcus agalactiae. Use of optochin
and bile soloubility tests to differentiate between viridans streptococci
and Streptococcus pneumoniae.
3
Corynebacterium
Use of Gram stain to characterize the morphology of
Corynebacterium. Use of Loefflers serum agar medium as growing
medium for corynebacteria. Elek's test and sucrose fermentation to
differentiate between Corynebacterium diphtheriae and diphtherioids.
4
Bacillus
Gram stains to characterize Gram-positive bacilli. Spore staining to
characterize the spores of Bacillus. Motility test and hemolysis on
blood agar to dipherntiate between Bacillus anthracis and Bacillus
cereus
5
Clostridium
6
Enterobacteriaceae
Gram stains to characterize Gram-positive bacilli. Spore staining to
characterize the spores of Clostridium. Nagler's reaction and Sormy
clot formation to identify Clostridium.
Gram stains to characterize Gram-negative bacilli. Oxidase test, nitrate
reductase test and oxidative fermentative test to identify
Enterobacteriaceae. Use of common selective and differential media to
differentiate between lactose and non-lactose fermenters.
7
Enterobacteriaceae
Use of Indole, methyl red, voges proskauer, and citrate "MViC" test to
differentiate between members of Enterobacteriaceae. Also use of
urease test, motility test and TSI agar test to differentiate between
Enterobacteriaceae.
8
1st examination
9
Pseudomonas
aeruginosa
10
Vibrio cholorae
11
Haemophilus
influnzae
Gram stains to characterize Gram-negative bacilli. Use of enriched
media "Chocolate agar" to grow Haemophilus. X and V factor test and
satalletism to identify Haemophilus.
11
Mycobacterium
tuberculosis
Acid fast stains to charactize acid fast bacteria "Mycobacterium". Use
of Lowenstein Jensen medium to grow of Mycobacterium
12
Medical Mycology
13
Applied
Microbiology
14
2nd examination
Gram stains to characterize Gram-negative bacilli. Oxidase test, nitrate
reductase test and oxidative fermentative test to identify
Pseudomonas. Use of selective "Cetermide" media to grow of
Pseudomonas and exhibition of exo-pigments on nutrient agar.
Liquefaction of gelatin.
Gram stains to characterize Gram-negative curved bacilli. Oxidase
test, nitrate reductase test and oxidative fermentative test to identify
Vibrionaceae. Use of common selective and differential media
"TCBS" to identify Vibrio cholorae and Vibrio parahaemolyticus.
Examination of yeasts and moulds (Candida & Asperigullus)
Microbiological examination of water and Milk
Spots