An Investigation into the Frequency of Occurrence of Asymmetric Pairs of Front Feet in Mature Equines Summary REASONS FOR PERFORMING STUDY: Asymmetric pairs of front feet have been identified using only visual assessments or comparison between the dorsal hoof wall angles of each foot in a pair. Existence of the condition is viewed as a potential unsoundness that may be a predictor of early pathology and lameness. Limited research, however, exists into how frequently the condition occurs in the mature equine, or what shape or forms it may take. AIMS: To identify in a limited population of adult horses the frequency of occurrence of asymmetric front feet, and any dimensional variations. MATERIALS AND METHODS: A study group of 40 (n=40), general purpose, mature horses was selected and measurements of both front feet taken. All of the study population had their front feet trimmed following a standard protocol. Four linear and one angular measurement of each front feet were taken from each of the equines using a digital vernier caliper, brass hoof gauge and a standard metric ruler. RESULTS: None of the total study population (n=40) demonstrated matching pairs of front feet. Four subjects demonstrated significantly different measurements between left and right feet. These differences where noted in the width of the hoof, distance between the heels and dorsal hoof wall angle. Length of foot and width of coronet appeared less relevant for asymmetry in this population CONCLUSION: Mismatched pairs of front feet occurred with a 16.66% rate of incidence within the study population. Sufficient evidence exists from the literature, to indicate the condition manifests itself as the result of skeletal asymmetry and the preference to use 1 one limb over the other. The syndrome appears to be multidimensional and cross species in nature. Using visual identification or dorsal hoof wall angle alone may be insufficient to accurately describe the condition. CLINICAL SIGNIFICANCE: A more comprehensive understanding of the influences for asymmetric front feet, and the conditions multidimensional nature, may lead to further research into trimming and shoeing protocols. These protocols should be bases on the biomechanical needs of the equine rather a preference to achieve an aesthetic appearance. Finally, objective information about the incidence of mismatched front feet may then allow research into whether the condition is associated with certain pathologies 1. Introduction Asymmetric pairs of front feet, (also referred to as mismatched hooves, uneven hooves or the “high/low” syndrome), have been identified both anecdotally and empirically (Bell 1996, Gonzales 1991). From a practical perspective this phenomenon is considered to be aesthetically displeasing by the horse owner, particularly in the show ring as it may have an affect on appearance to the judge, or for sale. In the athletic equine it is felt that the condition may affect the range or level of performance. From a veterinary and farriery perspective it is believed that the condition may be an indicator of underlying pathology, or unsoundness, or it may predispose the equine to future pathology (Wilson et al 2009.). Concerned owners and veterinarians often call upon the Farrier to effect change and produce a more aesthetically pleasing pair of front feet. This is done with the belief that the changes will potentially enhance quality and length of performance and minimise the risks of pathology. The usual approach to trimming and shoeing of these feet; is to trim the long heels of the steeper angled hoof and fit the shoe with width and length. This is believed to give this hoof a larger appearance and a similar angle to its contra lateral partner. The other hoof has no heel removed, and the hoof wall is trimmed to make it appear less acute in angle. The shoe is then fitted to the periphery of the hoof with the minimum of width and length to make it appear smaller (Butler 1985). These actions are 2 often undertaken without regard to the biomechanical needs of the individual hooves or the conformation of the limbs that they support and therefore the author considers these actions to be ill advised without a more in depth understanding of the condition. It should be also noted that, in many of the instances of attempts to “correct” the condition, the equine is usually performing to a reasonable level, with no sign of lameness or infirmity. Wilson et al (2009) postulated that some type of compensatory mechanism may exist to assist the equine to cope with the condition. Should this mechanism exist, then it is theorised that the trimming of the hoof to produce the aesthetically pleasing model, rather than for biomechanical efficiency, could interfere with, or remove that compensatory mechanism. The aim of this study was to identify the frequency of occurrence of asymmetric front feet in a limited population of adult, general purpose equines. It also aimed to identify to what differences may exist, and any variations of the condition. Once the condition is better understood, a suitable trimming and shoeing protocol could be devised. Further objective data may then allow other Farriers and Veterinary Surgeons, to conduct further research on how and why the condition exists. 1.1 The “Ideal” Foot The “ideal” equine foot is believed to fit within an acceptable range, rather than comply with an exact number (Craig 2009). The equine is usually regarded to be in the “normal” range, if the front foot is basically round, symmetrical in nature around the long axis of the limb, with an angle of between 50º and 55º (Hickman and Humphrey 2004, Floyd and Mansmann 2007). The angle of the equine foot is usually measured at the dorsal aspect of the hoof wall as demonstrated in figure eight, and this will be referred to as the Dorsal Hoof Wall Angle (DHWA) for the rest of this paper. Casonova and Oosterinck (2012) demonstrated in a population of young Catalan Pyrenean horses that, the width of feet and the length of foot were approximately the same, suggesting a relationship which could also be considered to be a “normal” observable fact whatever the size of the feet in 3 question. Further research work has suggested that ideal feet show quantitative geometric proportions (Balchin et al 2009). If this ideal is achieved, and the equine has symmetrical limb conformation, it should follow that pairs of front feet should follow the same principles and appear as a reasonably matched pair. Figure one demonstrates a pair of feet that, although they do not display perfect symmetry, are visually of similar angles and dimensions. Figure 1: A reasonably matching pair of feet? It is further expected that, even when pairs of feet do not fit into the “ideal” scenario, that they still should still exist with similar dimensions and angles. Wilson et al (2009) however noted in their study into skeletal asymmetry, that in 358 pairs of measurements (of front feet) taken only 11 instances demonstrated no difference between left and right measurements. Asymmetric pairs of front feet are usually reported as one hoof having a steep angle with a long heel (the “high” foot). This foot is often described as being “upright, “boxy” or “club” in relation to its partner, regardless of its actual angle. The other hoof has a more 4 acute angle and generally appears slightly larger (the “low” foot). Quite often the condition appears with one foot in the previously described ideal range, with the other having a more acute or steeper angle in relation to its contralateral partner. This is in contrast to what is regarded as an “ideal” pair of front feet which are expected to have similar dimensions. It should be pointed out that these asymmetrical feet often present with other characteristics. 1.2 The Steeply Angled or “High” Foot The terms “upright”, “boxy” and “club” footed are used liberally when asymmetric pairs of front feet are observed; to describe any foot with a steeper angle than its contralateral partner. Floyd and Mansmann (2007) states that when mismatched feet present, the condition (the steeper angled foot) should not be confuses with a “club foot”. Stashak (1976) defines an upright front foot as one with an angle between 55º and 60º, a boxy foot between 60º and 65º and a club foot as being greater than 65º. In asymmetric pairs of front feet, the steeper foot is often referred to as being the “more upright” (boxy or Club) foot, without regard to this definition. There is very little evidence to suggest that the more steeply angled foot does not still fall within ideal parameters. Redden (1992) describes a foot as being “upright” if it is more than three degrees steeper than its partner. This however may lead to an inaccurate evaluation of a pair of feet as it is being judged against its contralateral partner that may, in itself be outside the expected range. Thus, there are problems in definition of “upright” that derive from being relative (to the other foot), making any judgement subjective in nature. Other characteristics that may be displayed by the more steeply angled foot are; Long, sometimes contracted heels. A small, often atrophic frog. A concave slope on the dorsal wall. A deeply vaulted sole around the apex of the frog. 5 A flattened, compressed area of sole in the toe area. An idiopathic deterioration of the white line (seedy toe/white line disease) in the toe area. It should be noted that in some cases that a crena or dorsal notch can be observed on xray plates of these feet at the juncture of the distal border and dorsal surface of the distal phalanx. It should also be noted that despite demonstrating distinctly different characteristics, asymmetric pairs of front feet are still routinely identified by differences by eye or dorsal hoof wall angle alone. This is quite often done by comparing one foot against the other (i.e. a relative difference) without regard to any stable norm (an objective measure versus a population data set). 1.3 The Acute Angled or “Low” Foot This foot type displays a distinctly more acutely angled foot pastern axis in comparison with its contralateral partner. Quite often this is accompanied by a broken back foot pastern axis and other deformities usually identified with pairs of flat feet such as Collapsed or under-run heels Over-developed frog Long toe with a stretched white line in the toe area Bent or displaced bars A broken back foot pastern axis A particular characteristic of the flat foot in the mismatched hoof scenario is that it is usually has a toe-in conformation. This conformation defect may be a simple misalignment from the fetlock, or a more distinct base narrow deviation from the shoulder. As the limb with the flat foot is hypothesised to be longer than its partner, this may be an adaptation facilitate an easier break-over. 6 As with most pairs of feet of this nature there is a tendency for the problem to become exacerbated with time, particularly if there is a lack of appropriate trimming or lack of support at the caudal aspect of the hoof. The example in Figure two demonstrates the typical scenario. The right hoof appears to have a steeper dorsal wall angle than its collateral partner, and longer heels. The left hoof appears more acutely angled and wider than the right hoof. Figure 2: An obviously asymmetric pair of front feet 1.4 Environmental Influences The effects of the environment on the equine hoof are well documented (Bradley et. al. 2002, Reilly 2006). However any extremes of temperature, humidity, diet and general management would be expected to affect all feet in the same manner. The traditional preference of the owner/rider to lead, mount and generally work with the equine from the left side may have some significance should the study show a bias of one of the asymmetric feet (e.g. the steeper foot), being on one particular side. McGreevy and Rogers (2005) identified this preference to work from one side and considered that it may have a possible side bias. They went on to state that data from unhandled foal ay therefore be of particular value. It should also be considered that where the equine is working predominately on the road, that the camber may have some effect on weight distribution and result in disparity in hoof conformation between left and right feet. Any 7 effects caused by the camber, would however, be expected to be cancelled out should the subject return to working on an even surface. Most working Farriers will be familiar with the change in dimensions that are associated with feet that are subject to habitual shoe loss. These feet tend to become more upright. Almost stumpy in appearance due to the loss of horny wall often associated with premature shoe loss and the need to carry out some foot dressing in effort to replace the lost shoe. Should this habitual shoe loss be unilateral (i.e. affect only one foot), whilst the other foot retains its shoe, then a pair of feet will take on a mismatched appearance. Usually however, if the habitual shoe loss ceases, the affected foot will begin to take on a more matched appearance to its contralateral partner over a period of time. There is poor understanding however, as to why habitual shoe loss should affect only one foot and not both. Other examples of mismatched feet where observed by Ivan Bell FWCF (personal communication) during his study of Household Cavalry horses in Hyde Park Barracks in 1996. He noted that the right fore hoof was often slightly larger that the left and reasoned that it was due to the Farrier habitually dressing the left foot first, and then spending less time dressing the right hoof. This situation occurred when the Farrier was put under pressure to keep up with another Farrier who was fitting the shoes. This problem was overcome by the simple change of procedure of starting on the right foot on alternative occasions which resulted in situation being reversed. Other ways in which asymmetry may be introduced in the shoeing process is by the existence of a medio-lateral imbalance in the hoof. The imbalance may be due to conformational anomalies (e.g. an angular limb deformity), or by inaccurate dressing of he hoof during the shoeing process. Effectively the hoof is just a thick layer of skin (epidermis), with a form that is constantly being modified through growth, wear, compression and concussion, as well as any act of farriery (Gill 2007). Should the imbalance not be recognised, then over time it would be expected that the hoof will change in shape, (and possibly size). If the imbalance exists in only one hoof, then 8 predictably it will alter in relation to its partner as well as possibly causing pathology, which in itself may lead to changes in the configuration of the hoof. Whereas Farrier-related inducement of asymmetry should always be considered as a possible source of the condition, modern training methods tend to indoctrinate the Farrier into the production of symmetry and balance using consistent, definable parameters in pairs of hooves. This is therefore unlikely to be a significant contributor to the condition. 1.5 Pathological Acquired Asymmetry Acquired pathological conditions are another possible source of mismatched front feet. Changes in the size, shape and configuration have long been noted by both Farrier and Veterinarian (Pollitt 2001, Stastak 1976), as the result of trauma and certain chronic pathological conditions. These changes tend to be more apparent when the condition or its associated lameness, affect only one foot or limb (unilateral lameness). As the pathology progresses over time the affected hoof develops distinct characteristics of its own that set it apart from the (presumably unaffected) contralateral hoof (Floyd and Mannsmann 2007). The changes in the hoof may be due to an impairment of natural function associated with this acquired pathology, and/or a change in foot placement to compensate for the impaired function. The development of a difference may also be due to the unaffected, contralateral foot being subject to different stresses in effort compensate for the discomfort/pain in the opposing limb. In many incidences of chronic lameness there is if course the possibility of both situations contributing to the asymmetry. The example in Figure three illustrates the distortion that can occur (assuming the feet where reasonably matched to begin with) when pathology is involved. In this case, severe inflammation of the carpal joint has resulted in a misaligned lower limb. The resultant misplaced weight bearing (through the long axis of the limb) has apparently caused distortion in the conformation of the hoof capsule. The right foot may have become more acute in angle due to compensatory weight bearing. 9 Plate 3: Front feet asymmetry attributed to pathology. Severe pathology has affected the left Carpal joint which has led to a change in the conformation of the associated hoof capsule. One of the most common examples of this is the ossification of the contralateral cartilages (sidebone). In this example the contralateral cartilages lose there function as part of expansion/contraction mechanism of the hoof due to the chronic ossification process. Usually the condition affects only the lateral (contralateral) cartilage, causing the lateral aspect of the hoof capsule to become more upright and occasionally contracted (unilateral sidebone). The hoof then becomes asymmetrical in its own right in relation to its contralateral partner. Should the condition affect both the lateral and medial cartilages (bilateral sidebone), then the hoof tends to develop contracture at the heels and take on a more upright, almost “boxy appearance, which again makes it notably different from its partner. A more graphic example of hoof capsule deformity is seen in the equine with chronic laminitis (Pollitt 2001); where the pedal rotation is moderate to severe in one foot, but exists to a lesser degree (or not at all) in the other foot. The resulting malformation of the hoof capsule may produce distinct differences between any pair of feet involved. It 10 should be pointed out however, that for pathology to be suspected as the cause of any asymmetrical hoof pair, some evidence must first exist that the pair of feet in question where at some juncture reasonably well matched! 1.6 Unresolved Flexural Deformities Flexural deformities in the neonatal and immature equine are well documented (Curtis 1999, Redden 1992,). Should these deformities remain unresolved and persist into adulthood, then they will almost certainly have some direct influence on the shape and form of the hoof capsule. Flaccid tendons in infancy are known to predispose the equine to developing a broken back pastern axis (and associated flat feet). Unresolved flexor tendon contracture is known to contribute to the formation of a steeper foot angle (Floyd and Mansmann 2007). These authors attribute the formation of the upright (with a broken forward pastern foot axis), “boxy”, or clubfoot appearance to an unresolved flexural deformity in the juvenile equidae. Redden (1992) states that “club feet are characterised by irregular horn growth, usually of the front feet, with one foot being more involved than both”. He goes on to state that the condition may be inheritable or due to a nutritional deficiency. Figure 4 demonstrates an unresolved Superficial Digital Flexor Tendon contracture that has persisted into adulthood. The proximal and middle phalanges have been moved in a dorsoventral direction by the contracture, to place the pastern at an almost perpendicular angle to the ground. The distal phalanx (and its associated hoof capsule), have been also moved backwards sufficiently for the digit to mature with the hoof capsule at a steeper angle than that of its contralateral its partner. 11 Figure 4: The effects of contraction of the superficial digital flexor tendon. The contracture has moved the proximal and middle phalanges to a much steeper angle in this mature horse. The unresolved flexural deformity theory may give some explanation as to why some feet have a greater angle than their contralateral partners when some contracture is in evidence, despite not being classified as upright, “boxy” or club in appearance. However this theory does not explain (when the foot with the steeper angle has a more desirable conformation), why the contralateral foot has a more acute angle and is larger. It should always be considered that the more acutely angled foot may be the result of an unresolved flaccid tendon and the steeper angled foot is within the expected range. Further to this, there is no explanation as to why one limb is affected differently than the other, or not at all. 1.7 Limb Length Discrepancies Angular/flexural limb deformities are regularly identified by the Farrier when evaluating the subject prior to shoeing. Whereas it is usual to observe examples of similar angular/flexural limb deformities in pairs of front limbs, it is less common to observe a 12 relatively straight limb accompanied by a limb with deformities. It is also uncommon to observe a pair of limbs with different deformities. It is reasonable therefore, to consider that should asymmetrical pairs of limbs be in evidence, they will influence the associated hooves in a different manner. A study into skeletal asymmetry in the racehorse (Watson et al 2003); found that in 76% of the study group, the right third Metacarpal (MCIII) was greater in length than the left. It further found that only 4% had the left MCIII longer than the right. Only 20% of the study group had MCIII of a similar length. The study stated (after Fretz et al. 1984) that, since the growth in length of the third metacarpal had ceased after 10 weeks, the length of this bone should not be influenced by any subsequent training conditions. One would imagine that the difference in length between left and right MCIII, (in 16 of the 46 horses in the study group, the difference was as much as 5-9 mm), would in some manner affect the performance of the horses investigated. The study however, concluded that the differences in MCIII length appeared to not affect the performance of the study group, regardless of whether they ran clock-wise or anticlock-wise around a race track. Limb length discrepancy (LLD) has been shown to exist in the Human (McCaw et. al. 1991, Čuk et.al. 2001), the Canine and other mammals (Hackert et.al. 2008). LLD in the human is known to affect both the upper and lower limb (Čuk et.al. 2001), and is described as a limb length inequality (LLI) or anisomelia. It is subdivided into “functional” LLI, and “actual” LLI. Functional LLI indicates that although the limbs are comparable in length, they function in different ways to compensate for either a misalignment in the appendicular skeleton (usually the pelvis and lower limbs), or as a response to pain. Actual LLI indicates that total or segmental length of one limb is less that the other (Hemiatrophy), or greater than the other (Hemihypertrophy). Although there is no direct anatomical comparison between the lower limb of the human which is attached to the pelvis, and the equine forelimb which has no direct attachment to the spine, the biomechanical implications may be the same and worthy of further study. 13 It is clear from the research into Human LLI, and that of other mammals, that not only does LLI exist; it is cross species in nature and endemic within a given population. With this information and the limited study of Watson et. al. (2003), it should therefore be reasonable to hypothesise that LLI will exist within the equine population. Wilson et. al. (2009) examined the relationship between elbow height and “hoof spread” in the adult equine and found that an inverse relationship existed. It was concluded that of a pair of hooves, the one with the most “spread” was on a shorter limb, but the authors did not explain as to why this would be. Traditionally it has been suggested that the steeper angled hoof with the longer heels is a compensation for a shorter limb. However there appears to be a lack of scientific evidence to support this in the equine, and the findings of Wilson et. al. (2009) seems to contradict this traditional view. However given that, in the human the toe on the shorter limb is drawn back on the shorter limb (McCaw et. al 1991), should this happen in the equine, it could be postulated that the hoof capsule conformation and development of longer heels is a compensation for a shorter limb. Clearly the limited research to date and contradictions in the evidence give an area for further study. It must be considered therefore, that to work soundly (at least in the short term), and in a reasonably balanced manner, that equines have in some way developed some form of mechanism to cope with this discrepancy in the length between left and right MCIII. This coping mechanism may involve the development of mismatched pairs of front feet, where one foot has adopted a more upright conformation with a longer heel to counter the discrepancy in the possible shorter length of MCIII. Again there seems to be no explanation as to why the condition exists in the first place. 1.8 “Handedness” and the Preferred Lead Syndrome Much of the research into LLI’s draws attention of the preference of use of one limb over another, in the species studied (Equine, Human, and Canine). In the equine world, those 14 involved in the training and schooling of the equine often report that a particular subject performs better on one rein, and feels less agile or flexible on the other. Preference for using a particular limb as a priority over the other (either by preferring to use it first or more frequently than the other), has been documented by a number of authors. Gill (1996) noted in his observations of immature equines that when grazing, most of the subjects took a particular stance. He noted that in effort to reach the ground that the subject would habitually protract one limb in front or the body, and retract the other under the body to maintain tripodal support. He noted over a period of time that this protracted/retracted pattern in the subject usually involved the same “preferred” limbs. These observations where also noted by van Heel et. al. (2006) when examining conformational traits in the foal in relation to lateralised grazing behaviour and the development of uneven (mismatched feet). It was determined that about 50% of the foals developed a preference to protract the same limb and therefore develop uneven feet. It was found be van Heel et. al. (2006), in their study, that this preference was more predominant in foals with small heads; and this conformational trait may lead to the need to protract a specific limb to facilitate a comfortable grazing stance. The existence of “handedness” has been well documented in the human (McCaw and Bates 1991, Čuk et. al. 2001), and physiological differences in skeletal symmetry and behavioural compensations observed in response to this preference. This asymmetrical locomotion tends to manifest itself when the equine is put into training. Riders/trainers regularly report that young horses show reluctance to lunge or be ridden on a particular rein, or find it difficult to lead off with one particular leg. Indeed the usual purpose of training and schooling of the equine is to produce a more balanced subject with better symmetry of motion. This one sided preference tends to become less of a problem as the training and conditioning progresses. Should however, there be failure to reinforce this training, or should it cease altogether, then one-sidedness is usually noted to re-assert itself. 15 Mcgreevy and Rodgers (2005) studied lateralised behaviour in thoroughbred horses and identified that the lateralisation occurred on two levels, motor and sensory. Mcgreevy and Rogers (2005) further identified in their study of the motor lateralisation, (using standing observations of the horse), that 52% preferred to protract the left fore, 13% preferred to protract the right fore, and 41% demonstrating a non-significant (ambidextrous) preference. This study also gathered data over a limited age range of its subjects. It found that preference to protract one limb over the other demonstrated a statistically significant increase with the older (over two year old) horses, with the number of ambidextrous horses decreasing with age. It seems however, that despite showing lateralised motor behaviour from an early age, and the skeletal asymmetry that results from this preferred behaviour, the equine still manages to perform to a reasonable level without dysfunction at least in the short term. There is only weak evidence to suggest that the condition itself is a predictor of unsoundness, and much more research is required in this area. 2. Materials and Methods 2.1 Selection Criteria for the Study Population A total of 40 (n=40), general purpose equines were selected (as listed below) from the author’s business in the North East of England between May and December 2011 to form the study population. The study population was subdivide into shod (n=30) and unshod (n=10) subjects to ascertain whether the attachment of a shoe had any influence on the outcome of the condition. The selection criteria used were as follows All subjects were between the ages of four and twenty. 16 All shod subjects were have been reshod on an approximately 6 week basis for the last 12 months (from shoeing records), and every eight weeks or less for the unshod subjects. None of the subjects had any significant history of shoe loss within the previous 12 months (this was considered as it is often noted that habitual shoe loss from one hoof causes it to become smaller and steeper in angle). None of he subjects were lame at the time of the study, and have no known history of lameness in the previous 12 months. None of the subjects should have any pre-diagnosed hoof pathology All subjects were in regular work, and be involved in a broad range of activities on varying types of surfaces (including the unshod subjects). It should be noted that differences in the colour of the front feet, or patterns of colour, where not considered to be relevant in the selection as it is believed that pigmentation does not heave any direct effect on the conformation or soundness of the equine (Pollitt 2001, Reilly 2004). 2.2 Foot Trimming Protocols All feet were trimmed to the same protocols described by Caldwell et al 2010 (after Savoldi 2006). White line exfoliated to reveal the sole and horny wall interface. Removal of the remaining exfoliating solar horn to reveal the true solar plane. 17 The bars were trimmed to normal proportions, removing only damaged or weak horn. The frog was trimmed back to live tissue and proportionate to the foot. Excess hoof wall at the bearing border was removed to a horizontal solar plane. The heels were trimmed approximately to the widest part of the trimmed frog. Figure 5: The hoof exfoliated prior to trimming. The proposed trim line is shown in red (after Savoldi 2006). It should also be noted that the front feet where trimmed on an alternative left/right foot first basis to avoid any bias (Bell 1992) 18 2.3 Materials Used The following were be used to take measurements and record the data; A 15cm (six inch) steel ruler and marker pen was used to inscribe lines on the hoof at the positions that were to be measured. A standard brass hoof gauge (marked in degrees). A digital vernier calliper (measurements in mm). Callipers Excel spreadsheet packages were used to record the data. Figure 6: Equipment used to carry out measurements of the hooves of the study population. 19 2.4 Foot Measurements Taken The following measurements of both front feet were taken and recorded after trimming. All angles where recorded in degrees, and all distances where recorded in millimetres. Each hoof was measured medio-laterally at the widest point of the foot (WOF) on the solar surface (measurement A in figure seven). Each hoof was measured at its longest point (LOF) from the apex of the toe through the frog to a line drawn between the lateral and medial heels at the widest point (measurement B in figure seven). Each was measured was taken between the lateral and medial heels (DBH) at the widest point (measurement C in figure seven). Each hoof was measured at the widest part of the coronet (WAC) (measurement D in figure eight). The angle of the hoof at the dorsal wall (DHWA) was measured in degrees (measurement E in figure eight). 20 C A B Figure 7: Measurements taken of the solar surface of the hoof capsule. Measurement A: Width of Foot (WOF). Measurement B: Length of Foot (LOF) Measurement C: Distance Between Heels (DBH). D E Figure 8: Measurements taken of the dorsal wall and the coronet. Measurement D: Width Across Coronet (WAC). 21 Measurement E: Dorsal Hoof Wall Angle (DHWA). In addition to the foot measurements taken, the following data was also recorded on the spreadsheet: The height of each subject in centimetres from the passport. An estimation of weight of using a calibrated weight tape around the thorax. The breed/type of the subject from the passport. 2.5 Data Analysis In the study a total 320 morphometric and 80 angular measurements where taken of the left and right front feet of 30 shod horse (Appendix I), and 10 unshod horses (Appendix II). All data was recorded on a Microsoft Excel™ spreadsheet. The data was then separated into that taken for left and right (shod horses Appendix Ia and Ib; unshod Appendix IIa and IIb). The mean, median, maximum, minimum (range) and standard deviation where calculated and are summarised in the table one (all shod and unshod horses), and table two (data split between left and right feet). Using the data, scatter graphs comparisons were done of left against right for both shod and unshod results and are shown in tables’ three to seven. Frequency histograms where produced to evaluate distribution of data using Minitab 15™ and are shown in Appendix III (a) and (b). A Chi square test was used to evaluate the fitness of the data summary in table eight. A paired t test was used to compare any relationships between the hoof measurements, and any differences or similarities between the shod and unshod study groups. 22 3.0 Results 3.1 Results of the Study Most previous published data has relied on trimming being carried out by “experienced Farriers” with no apparent specific protocol being followed. Further to this, the practical aspects of the accuracy of the data collection receive little mention. As this authors data was collected from live (in vivo) horses (usually in stable yards); and there being no apparent precedent, it was considered that the margin for error in the measuring protocols for use in the descriptive data with regard to differences in metric measurements between front feet should be 5mm. Any measurement difference of 5mm or less would be classed as within the normal range. Any difference greater than 5mm would be considered to be notable. Given the accuracy of the method used to measure DHWA and the conditions in which it was done the author determined, as per Redden (1992) that differences in angles of three degrees or less would be considered within the normal range. Table 1: Summary of means, standard deviations and ranges measured of shod and unshod subjects. Shod horses Unshod (n=60) horses(n=20) Mean ± s.d (mm) Range (mm) Mean ± s.d (mm) Range (mm) WOF 131.12 ± 8.08 114.34-145.80 120.75 ± 13.14 97.62-140.62 LOF 130.29 ± 8.85 116.93-152.15 119.84 ± 13.36 97.66-145.47 DBH 70.43 ± 10.16 53.21-97.92 62.44 ± 13.63 48.26- 87.12 DOC 113.65 ± 6.11 98.47-126.39 104.21 ± 10.23 87.80-120.99 DHWA 52.82 ± 2.49(º) 48.00-59.00(º) 54.75 ± 2.17(º) 51.00-59.00(º) 23 Table 2: Comparison of mean data from left and right feet of all subjects. Left Foot Right foot (n=30) (n=30) Mean ± s.d (mm) Range (mm) Mean ± s.d (mm) Range (mm) WOF 130.74 ± 8.57 114.34-145.80 131.50 ± 7.68 115.64-145.10 LOF 129.75 ± 9.14 116.93-152.15 130.83 ± 8.67 119.41-151.36 DBH 70.25 ± 10.14 53.21-97.92 70.62 ± 10.35 55.00-97.08 DOC 113.07 ± 5.98 100.92-124.53 114.22 ± 6.27 98.47-126.39 DHWA 52.80 ± 2.63 48.00-59.00 52.83 ± 2.39 48.00-59.00 Unshod (n=10) WOF 120.70 ± 13.32 97.62-140.62 120.89 ± 13.69 97.72-139.33 LOF 118.61 ±13.36 97.66-142.46 121.07 ± 13.96 98.45-145.47 DBH 61.82 ± 12.92 48.26-82.47 63.03 ±14.98 48.24-87.12 DOC 104.20 ± 10.34 87.80-119.43 104.23 ± 10.67 88.57-120.99 DHWA 54.65 ± 2.01 52.00-58.00 54.90 ± 2.42 51.00-59.00 Shod (n=10) Width of Foot Of all the widths measured of both left and right front feet, the maximum was145.80 mm, and the minimum was 114.34 (mean width 131.12). Of these feet, 24(80%) subjects had a difference in width of 5mm or less. 6 subjects (20%) had a difference greater that 5mm between hoof widths, and this can be seen on the table three scatter graphs Of the 5 feet with the disparity of greater than 5mm, 4 subjects (nos.4, 7, 9, 27) had a wider right foot. Two subjects (nos. 12, 18) had a left foot wider than the right. Notably, of these 5 subjects, only 2 (nos. 18 and 27) demonstrated a foot angle difference of more than 3º. The frequency histogram displayed an even distribution of data (P = 0.271) 24 Of the measurements taken of unshod subjects, the maximum width was 140.62 mm and the minimum was 97.62mm (mean width 120.79). Only one subject (10%) demonstrated a difference in width between left and right feet; and in this case (no10) the right demonstrated the greater measurement. This subject demonstrated no notable difference in hoof angle. The frequency histogram displayed an even distribution of data (P = 0.267) Table 3: Scatter graph comparisons of X v Y for width of foot for shod (a) and unshod (b) subjects. Scatterplot of left WOF vs right WOF Scatterplot of left WOF vs right WOF 150 140 130 left WOF left WOF 140 130 120 110 120 100 110 115 120 125 130 right WOF 135 140 145 100 (a) Shod Feet. 110 120 right WOF 130 140 (b) Unshod feet. Length of Foot Of the lengths of foot measured in the shod subjects, the maximum was152.15mm and the minimum was 116.93mm (mean length 130.29mm). Of these, three subjects (10.00%) demonstrated foot length differences of more than 5mm. Of these, two subjects (nos.3 and 28) had the right foot longer than the left, and one (no. 4) had the left foot longer than the right. Neither of these subjects demonstrated any notable differences in dorsal wall hoof angle. The frequency histogram for this set of data was not normally distribute and was skewed right (P = 0.011), however the data set was transformed using a log10 conversion to produce more normally distributed data (P = 0.024) 25 Of the measurements taken in the unshod subjects, the maximum length was 145.57mm and the minimum was 97.66mm (mean width 119.84). Only one subject (10%) demonstrated a difference of length between left and right feet; and in this case (no.8) the right demonstrated the greater measurement. This subject demonstrated no notable difference in hoof angle. The histogram data for this measurement was normally distributed (P = 0.186) Table 4: Scatter graph comparisons of X v Y for length of foot for shod (a) and unshod (b) subjects. Scatterplot of left LOF vs right LOF Scatterplot of left LOF vs right LOF 155 140 150 145 130 left LOF left LOF 140 135 130 120 110 125 120 100 115 120 125 130 135 140 right LOF 145 150 155 (a) Shod feet. 100 110 120 right LOF 130 140 (b) Unshod feet. Distance between heels Of the measurements taken of shod subjects, between the heels the maximum was 97.92mm and the minimum was 53.21mm (mean distance 70.43mm). Of the subjects measured nine (30%) demonstrated a disparity in distance between the heels of greater than 5mm. Of these, six (nos. 1, 7, 16, 20, 22, 27) had a greater distance between the heels on the right foot, and three (nos.12, 13, 18) had a greater distance on the left foot. Of these nine subjects, three demonstrated a disparity of more than 3º in the dorsal hoof wall angle. The data demonstrated even distribution (P=0.239) Of the measurements taken of unshod subjects, the maximum distance between the heels was 87.12mm and the minimum was 48.26mm (mean distance 62.44). Only one subject 26 150 (10%) demonstrated a disparity of distance; and in this case (no.10) the right demonstrated the greater measurement. This result differed from that of the shod subjects and the data was not evenly distributed (P=0.011) Table 5: Scatter graph comparisons of X v Y for distance between heels for shod (a) and unshod (b) subjects. Scatterplot of left DBH vs right DBH Scatterplot of left DBH vs right DBH 100 85 80 90 80 left DBH left DBH 75 70 70 65 60 55 60 50 50 50 60 70 80 90 100 50 right DBH (a) Shod feet. 60 70 right DBH 80 (b) Unshod feet. Width across Coronet Of the measurements taken of the widest part of the coronet band, the maximum was 126.39mm and the minimum was 98.47mm (mean distance 113.65). Of the subjects measured, two (6.66%) demonstrated a disparity of more than 5mm. both of these subjects (nos. 14, 20) demonstrated the larger measurement on the right foot. Neither of these subjects demonstrated a significant difference in hoof angle. The data was normally distributed (P=0.874). Of the measurements taken of unshod subjects, the maximum distance at the widest part of the coronet was 120.99mm and the minimum was 87.80mm (mean distance 104.21). None of the subjects demonstrated a measurement of more than 5mm. the data was normally distributed (P=0.332). 27 90 Table 6: Scatter graph comparisons of X v Y for width across coronet for shod (a) and unshod (b) subjects. Scatterplot of left WAC vs right WAC Scatterplot of left WAC vs right WAC 125 120 120 115 left WAC left WAC 110 115 110 105 100 95 105 90 100 100 105 110 115 right WAC 120 125 130 (a) Shod feet. 90 95 100 105 110 right WAC 115 120 (b) Unshod feet. Dorsal Hoof Wall Angle Of the angles measured, the maximum angle was 59º and the minimum angle was 48º (mean angle 52.82º). 3 subjects (10%) demonstrated disparity of hoof angles of more than 3º. Of the 27 subjects showing angle differences of less than 3º, 8 subjects (26.66%) had no difference in hoof angle at the dorsal wall. Of the three feet with angle differences of more than 3º, two subjects (nos.12 and 18) had a steeper angle on the right foot. Only one subject (no.27) had a steeper angle on the left foot. The data was not normally distributed (P=0.049), but was subjected to a Log10 conversion (P=0.73). Of the angles measured, the maximum was 59º and the minimum was 51º (mean angle 54.75). Only one subject displayed a disparity of angle of more than 3º; and in this case the right demonstrated the steeper angle. The data was normally distributed (P=0.560). 28 125 Table 7: Scatter graph comparisons of X v Y for dorsal hoof wall angle for shod (a) and unshod (b) subjects. Scatterplot of left DHWA vs right DHWA Scatterplot of left DHWA vs right DHWA 60 58 58 57 56 left DHWA left DHWA 56 54 52 50 55 54 53 52 48 48 50 52 54 right DHWA 56 58 60 50 51 52 53 54 55 right DHWA 56 57 58 3.2 Summary of results It was noted in the results that the four (3 shod, 1unshod) subjects displaying a dorsal wall angle disparity of greater than 3º, also displayed a measurement difference of more than 5mm at the width of foot and heel distance (nos. 12, 18, 27). No subject displayed hoof angle disparity without a difference in the other measurements. This seems to suggest that there is a relationship between dorsal wall angle, foot width and heel distance. Further to this, three subjects (nos. 4, 7, 20) displayed a disparity of measurement of more than 5mm without a significant difference in dorsal wall angle. Seven subjects displayed disparity of more than 5mm in only one measurement. Fifteen subjects displayed no significant difference in any of the measurements taken. Within this study it was found that a mean angle of 53º ± 2.49° existed for all the shod subjects; and 54º ± 2.17° for the unshod horses (although it should be noted that this was a relatively small sample size). Other texts (Floyd and Mansmann 2007) have demonstrated similar results and variably concluding that the expected normal range of hoof angles to be between 50-55º. 29 59 Table 8: Summary of results, (summary of unshod subjects in brackets) WOF LOF DBH WAC DHWA 1 3 0 1 Right greater 4 2 6 2 2 measurement (1) (1) (0) (1) 3 (10%) 9 (30%) 2 (6.66%) 3(10%) (1) (1) (1) (0) (1) 7 (17.5%) 4 (10%) 10 (25%) 2 (6.66) 4 (10%) Left greater 2 measurement Total (1) larger 6 (20%) measurement Shod (unshod) Combined study (n=40) A Chi-Square goodness of fit test for observed counts was carried out on the data in table eight using Minitab 15™ and the following results were recorded Using category names in Type Category WOF LOF DBH WAC DHWA N 27 DF 4 Observed 7 4 10 2 4 Chi-Sq 7.25926 Test Proportion 0.2 0.2 0.2 0.2 0.2 Expected 5.4 5.4 5.4 5.4 5.4 Contribution to Chi-Sq 0.47407 0.36296 3.91852 2.14074 0.36296 P-Value 0.123 The observed values for WOF and DBH are greater than the expected values of these measurements, demonstrating that the Null Hypothesis (that pairs of feet are the same) is not proven. LOF and DHWA are lower than expected, but only by a small margin. WAC is much lower that expected, and this may be due to the relatively small population sample and it is suggested that the study be conducted with a larger sample to confirm this finding. However the P value of 0.123 indicates a reasonable level of confidence in 30 the data. The overall data appears to suggest that WOF and DBH are more significant when attempting to identify and describe the condition, whereas LOF, WAC and DHWA have a lower significant relevance. It appears from the table that in the shod horse study that were a greater measurement is found, it is predominately the right foot that displays the greater measurement on a 2/1 ratio. In the study of the unshod subjects, despite the small study group, where a disparity in measurement of greater than 5mm exists, it was found to be in the right foot. This suggests that, in this study, where foot size disparity does exist it is predominately the right foot that is larger than the left. In this study only 4 subjects (2 shod, 2 unshod) presented with pairs of feet with hoof angles greater than 55º. Only one subject (shod) presented with a pair of feet with angles of less than 50º. Of the three subjects that presented with hoof angle disparity of more than 3º, none of the steeper angled feet demonstrated a hoof angle of more than 55º, and therefore it may be proposed that these steeper angled feet fall within the expected normal range. Two of these subjects demonstrated hoof angles in the lower angled foot of 48º and it may be proposed that they are not within the expected range. Interestingly, the three shod subjects that had the greatest disparity in dorsal wall angle also demonstrated a disparity greater than 5mm in the width of the hoof, and the distance between the heels. The greater measurements (foot width, heel distance) were consistent with the foot with the lower angle. Of the 30 shod subjects, 10 demonstrated differences in the other foot measurements but had dorsal wall angles that were the same or less that 3º. 4.0 Discussion of Results Prior to this study there is limited evidence of studies that attempt to identify the frequency of occurrence of asymmetrical pairs of front feet, or quantify any dimensional differences of the condition in an adult population of horses. Most texts (Stashak 1976, Floyd and Mansmann 2007) make empirical reference to asymmetric pairs of front feet by making comparisons between the DHWA of each foot, but make no reference to 31 population traits, or any other dimensional parameters. Curtis (2011) visually identified frequency of unilateral club foot and flexural contracture in a population of juvenile thoroughbreds over a number of years, but made no quantitative measurements of the feet themselves. Kroekenstoel et. al. (2006) observed asymmetric pairs of front feet in a population of Dutch Warmbloods at 27 and 55 weeks and noted that the subjects showed a significant difference in distal limb loading. Although pressure measurements and radiographic analysis where evaluated, there was only limited reference to the dimensions of the feet themselves or any population traits. Previously, asymmetric pairs of front feet have been identified by visually distinguishing one foot as being upright, “boxy” or club in comparison to its contralateral partner, without reference to any measured parameters. The condition has also been identified (Redden 1992) by making comparison measurements between the hoof angles at the dorsal wall, but again referring to the steeper angled hoof as being upright, “boxy” or club in comparison to its contralateral partner, regardless of the angle of the other hoof (and without reference to any theoretical norm), or any other measured parameter. Whereas there are a number of studies that relate the condition to skeletal asymmetry (Kroekenstoel et. al. 2006) and behavioural preferences (van Heel et al. 2006). There appears to be limited attempts to actually quantify the affected feet using morphometric measurements other than DWHA. Ducro et. al. (2009), using data from the Royal Dutch Warmblood Studbook, and the Royal Dutch Equestrian Sports Federation records, noted a prevalence of the condition of over 8% from 2000 onwards. The subjects where scored as either having “uneven feet”; or not. Other than some mention of relative heel height and hoof shape, it appears that, again no other quantitative measurements where taken. Another study carried out by van Heel et al. (2006) noted that in 24 foals studied, 46% of the group developed a preference at 27 weeks to protract one limb. It was noted that these subject had smaller heads and where higher at the withers than the rest of the study group, and went on to develop “uneven feet” by 55 weeks. This study did use hoof angle and force plate 32 analysis to describe the differences, but took no other quantitative measurements of the foot itself. It was noted that various different methods of evaluation where used in previous studies to identify the existence of the condition, and that may account for the range of results that were found in table nine. Curtis (2011) used a visual assessment to identify unilateral club foot juvenile thoroughbred foals. Kroekenstoel et. al. (2006) stated that visual assessment was a subjective procedure, and, concluded that it was not sensitive enough to appreciate the alignment of the distal limb as it did not correspond with radiographic measurements taken. The results of this study indicate that asymmetric pairs of front feet have an incidence of occurrence of 16.66% within this limited study population. Since other studies compared in table nine show similar results, it may be reasonable to assume that differences in the dimensions between pairs of front feet are the norm within the wider population. None of the subjects in the limited study carried out by the author demonstrated the extreme dissimilarity that has been anecdotally described (Gonzales 1991). It may therefore be prudent to repeat the study with a larger population of equines to confirm the result of this and other studies. Table 9: Results comparison of frequency of occurrence from previous studies showing outcomes in relation to population size, and methods used. Author(Date) Population n= (no. studied subjects) of Measurements used % identified with disparity Storey (2012) Mature general 40 As per methods 16.66%. (This Author) purpose. in this paper. Curtis (2011) Thoroughbred 373 foals. Kroekenstoel Warmblood 23 Visual 19% (identified interpretation. as ULCF). Visual 33 and 6.5% (visual) et. al. (2006) (between 27 radiograph. 49% and 55 weeks). Ducro Bovenhuis Mature Dutch 44,840 et. Warmbloods. al. (2009) Ducro. (radiograph). (from Uneven feet 8% from 2000 stud (0/1= n/y). onwards. books) Mature Dutch 23,116 Gorissen et. al. Warmbloods. Uneven feet 6.20% (basic). (0/1= n/y). 5.78% (elite). (2009) Van Heel et. al. Dutch (2006) DHWA Visual 48%. Warmbloods from foals. photographs. van Heel et. al. Dutch (2010) 24 17 DHWA Warmblood (3- MetronPX y-o). software. using 25%. The data collected from the limited population in this study indicates that disparity in foot size was present in 10% of the subjects if DHWA alone is used, and rose to 16.66% if WOF and DBH are include as part of descriptive criteria. Both shod and unshod horses where measured in the study to evaluate whether shoeing has any overt affect on the presentation of the condition. Although the two study groups demonstrated similar trends in regard to WOF, LOF, DAC and DHWA; it was noted there was a comparative disparity in DBH. Whereas nine (30%) of the shod subjects displayed notable differences in DBH, only one (10%) of the unshod study group demonstrated the same difference. As the unshod study group was small in comparison to the shod study group, it was decided that no conclusions could be draw about the effects of shoeing on the condition, without gathering more data measurements on an unshod horse population with which to make comparison. Within the study population it was noted that, when on foot had a notably larger measurement, or a more acutely angled foot, it was the right foot that displayed this tendency as summarised in table eight. Watson et. al. (2003) stated that “in man, the 34 dominant right hand is often larger than the non dominant left hand”. The previous anecdotal descriptions of the condition have referred to one foot appearing larger than the other; there is no reference as to which is predominately the largest of the pair. Although the measurements appear to suggest an inclination for the right foot to be the larger foot; the limited size of the study population and the lack of previous study in this area, make it difficult to draw any definite conclusions from this information. The author however, suggests that this is another area for further study. Most of the authors cited seem to come to the conclusion that the development of asymmetric pairs of feet first present in the juvenile equine. Curtis (2011) evaluated most of his thoroughbred foals at 4 week after birth and noted that various limb deformities, including a unilateral club foot (ULCF) could present at this early stage. Kroekenstoel et. al. (2006) looked at Warmblood foals at 27 to 55 weeks and observed changed in loading patterns between the asymmetrical pairs of feet, indicating that the condition was present in that time period. van Heel et. al. (2010) noted that asymmetrical feet recorded in the juvenile warmblood could still be observed in the three year old subject. He also noted that not only did the condition appear to persist as the subjects matured, the incidence actually increased with age. Most of the authors are in agreement that the condition of asymmetrical front feet is a manifestation of skeletal asymmetry. They further suggest that this is a result of a preference to use one side over the other (“handedness”). Kroekenstoel et. al. (2006) suggested that juvenile equines with proportionally longer legs and small head may be more susceptible to the development of the condition. Opinion as to the conditions affect on performance or as predictor of unsoundness is divided. None of the authors work cited, make any reference to actual trimming or shoeing protocols in relation to soundness. Asymmetric pairs of front feet may be the result of an orthopaedic developmental disorder (unresolved flexural deformities/limb length discrepancies, skeletal asymmetry) and should be considered a potential issue alongside angular/flexural/rotational limb 35 deformities. The condition appears to begin to manifests itself in the post partum foal that has started to graze. The preferred (protracted limb) stance is probably the initiating factor in the development of the condition; As it is generally accepted that the Farrier cannot change the conformation of the mature equine, once the horse is passed the age that the epiphyses have ceased to grow, it can be postulated that very little can be done to change the condition. It is clear from both Curtis (2001) and Kroenstal that asymmetric pairs of front feet appear in the juvenile equine with varying rates of prevalence. van Heel et. al. (2010) notes that the condition not only persists in three year old Dutch Warmbloods, but the may become more accentuated as the subject approaches maturity. Both Ducro et. al. (2009) and the author’s studies demonstrate that the condition persists into adulthood. It is unclear as to whether this persistence is due to a failure to resolve the condition before maturity is reached, or whether resolution has been attempted and been unsuccessful. If the results of this study and others are demonstrative of the general (mature) equine population, it should be considered that the condition in itself may not be resolvable. If the equine manages to work soundly and evenly, at least on a limited basis, it can be theorised that the subject has developed a mechanism which allows it to compensate for any asymmetry of anatomy or locomotion. It may be further theorised therefore that the asymmetric pairs of front feet are part of compensatory mechanism, however much more research needs to be done on a larger equine population to confirm any findings. . 5.0 Conclusion Asymmetrical pairs of front feet appear to occur with a varying degree of prevalence in different equine populations. This study of this relatively small population, using five measurements, found that 16.66% frequency of occurrence of asymmetric pairs of front feet in the mature equine. Curtis (2011), using visual assessment, found a higher prevalence of occurrence in juvenile Thoroughbreds. It is may be hypothesised that this higher prevalence is related to the subjectiveness of visual assessment as described by 36 Krokenstal et. al. (2006), or may possibly be a population specific effect (i.e. using only one breed or type of subject; in this instance the Thoroughbred). Ducro et. al. (2009) identified a lower frequency (8%) of occurrence in Mature Dutch Warmbloods between 2000 and 2010 using available records. Since the subjects where scored as having, or not having “uneven” feet, rather than having morphometric measurements taken, the results may be also be considered to be subjective. This study, although it had a high number of subjects, was also population specific. Some of the population in this authors study displayed notable differences between foot measurements as well as hoof angle. Others displayed notable differences in foot measurements but had hoof angles that where similar or the same. The data therefore suggest that the mismatched foot condition exists in more than one form and this requires more study. 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