genotype frequencies
-0.2
0.2
0.6
1.0
Figure S1. Genotype frequencies according to parental allelic state across the F2 S. aethnensis and S. chrysanthemifolius genetic map and chi square test results
against expectations of mendelian segregation.
XX XX
* *** * *
X
* *
*
**
* *
* *
LG1
LG2
genotype frequencies
-0.2
0.2
0.6
1.0
scaled map distance
*
*
*
* *
LG3
LG4
LG5A
LG5B
genotype frequencies
-0.2
0.2
0.6
1.0
scaled map distance
* * *** * *
LG6
LG7A
*
LG7B
LG8A
LG8B
LG9
***
**
LG10A
*
LG10B
scaled map distance
Notes. The x axis indicates the genetic map with x axis labels referring to the linkage groups in Figure 1. Boundaries between linkage groups are represented by
vertical black dotted lines. “A” and “B” following linkage group names indicate the upper and lower unlinked regions of the same chromosome shown
vertically aligned in Figure 1. The same map scale was used for each figure panel. The y axis indicates per locus genotype frequencies. The central grey band
indicates expected genotype frequencies (1:2:1 ratio). The lower and upper lines and points indicate homozygous S. aethnensis and 1-S. chrysanthemifolius
genotype frequencies respectively. Only loci whose inheritance allowed different heterozygous and homozygous genotypes to be distinguished are presented.
Asterisks indicate significantly non-mendelian genotype segregation according to chi square tests at a 5 % per locus confidence level. X indicates that the
heterozygote genotype class was significantly under-represented according to chi square tests of loci where homozygote and heterozygote genotypes could be
distinguished. Black shaded asterisks and x symbols indicate significant additional segregation distortion of genotypes and heterozygotes following tests against
a null hypothesis segregation distortion caused by observed post-zygotic mortality before flowering or failure to flower.
genotype frequencies
-0.2
0.2
0.6
1.0
Figure S2. Genotype frequencies according to parental allelic state across the F2 S. aethnensis and S. chrysanthemifolius genetic map separated according to
parental cytotype and chi square test results against expectations of mendelian segregation.
** ***
*** ** **
C
ACA
CA
A
C
A
C
A
CA
CA
C
A
C
** **
A
C
A
C
**
** *
A
C
C
A
C
A
A
C
* **
* *
C
A
C
A
C
A
C
A
C
A
C
A
C
C
A
A
A
C
A
C A
C
C
A
LG1
LG2
genotype frequencies
-0.2
0.2
0.6
1.0
scaled map distance
**
*
C
A
A
C
A
C
A
C
* * *** **
C
A
A
C
*
C
C C A
A A
AAAA
CCC
CC
A
C
A
CA
C
A
A
CA
C C
C
A
LG3
C
A
C
A
A
C
A
C
A
C
C
A
C
A
C
A
LG4
LG5A
C
C
A
A
C
A
C
A
LG5B
genotype frequencies
-0.2
0.2
0.6
1.0
scaled map distance
** ** **
*
C
A
*
C
A
C
CCC
AAA
CC
AA
A
CA
A
CC
LG6
**
*
A
C
A
A
C
C
A
LG7A
C
A
A
C
AC
LG7B
A
C
C
CC
C
A
CC
C
CA
AA
CA
A
A
A
A
ACAA
CCA
C
CCC
LG8A
A
C
A
C
C
A
C
A
LG8B
A
C
CA
LG9
***
*
A
CC
CC
AA
AA
C
C
A
A
C
A
A
C
C
A
C
A
CA
CA
CAA
A
C
A
C
LG10A
LG10B
C
scaled map distance
Notes. See notes to Figure S2. Lines with points labelled as “A” or “C” represent genotype frequencies of mapping individuals with S. aethnensis and S.
chrysanthemifolius cytotypes, respectively. Lower and upper rows of asterisks indicate significantly non-mendelian genotype segregation according to chi
square tests for individuals with S. aethnensis and S. chrysanthemifolius cytotypes, respectively. Black shaded asterisks indicate significant additional
segregation distortion of genotypes following tests against a null hypothesis segregation distortion caused by observed post-zygotic mortality before flowering
or failure to flower.
-0.2
allele frequencies
0.2
0.6
1.0
Figure S3. Allele frequencies according to parental state across the F2 S. aethnensis and S. chrysanthemifolius genetic map and chi square test results against
expectations of mendelian segregation.
AA AA
A
A
A
A
A
* **
*
*
*
*
*
LG1
LG2
scaled map distance
-0.2
allele frequencies
0.2
0.6
1.0
C
*
LG3
LG4
LG5A
LG5B
-0.2
allele frequencies
0.2
0.6
1.0
scaled map distance
LG6
LG7A
LG7B
LG8A
LG8B
LG9
AA
A
**
*
LG10A
LG10B
scaled map distance
Notes. See notes to Figure S2. The y-axis indicates per locus allele frequencies. The central grey line indicates expected allele inheritance (1:1 ratio). Line and
points indicate frequencies of S. aethnensis and 1-S. chrysanthemifolius alleles below and above the line, respectively. Only loci whose inheritance allowed
different maternal and paternal alleles to be distinguished are presented. Asterisks indicate significantly non-mendelian allelic inheritance according to chi
square tests at a 5 % per locus confidence level. Letters “A” or “C” indicate if S. aethnensis or S. chrysanthemifolius alleles are significantly under-represented,
respectively. Black shaded asterisks and letters indicate significant additional segregation distortion of alleles following tests against a null hypothesis of
segregation distortion caused by observed post-zygotic mortality before flowering or failure to flower.
AA AA
AA AA
C
A
CA
A
C
CA
A
A
A
A
A
A
A
A
A
C
A
C
C
A
C
A
C
A
-0.2
allele frequencies
0.2
0.6
1.0
Figure S4. Allele frequencies according to parental state across the F2 S. aethnensis and S. chrysanthemifolius genetic map separated according to parental
cytotype and chi square test results against expectations of mendelian segregation.
C
A
C
A
LG1
LG2
allele frequencies
0.2
0.6
1.0
scaled map distance
A
C
A
C
A
C
A
CA
C
A
C
C
A
A
C
A
A
C
C
A
-0.2
A
LG3
LG4
LG5A
C
A
LG5B
-0.2
allele frequencies
0.2
0.6
1.0
scaled map distance
AA
AA
C
A
C
A
LG6
LG7A
A
C
LG7B
CC
A
CA
CA
A
LG8A
C
A
LG8B
LG9
CCC
AAA
LG10A
A
C
C
A
LG10B
scaled map distance
Notes. See notes to Figures S2 and S4. Lines with points labelled as “A” or “C” represent genotype frequencies of mapping individuals with S. aethnensis and
S. chrysanthemifolius cytotypes, respectively. Lower and upper rows of letters at the top of plots indicate significantly non-mendelian allelic segregation with S.
aethnensis (A) or S. chrysanthemifolius (C) alleles being under-represented according to chi square tests at a 5 % confidence level for individuals with S.
aethnensis and S. chrysanthemifolius cytotypes, respectively. Black shaded asterisks and letters indicate significant additional segregation distortion of alleles
following tests against a null hypothesis of segregation distortion caused by observed post-zygotic mortality before flowering or failure to flower.