FOLATE DEFICIENCY Measures and criteria used to define biochemical variables of the study population Variable Indicator Population or Individual* Folate deficiency Serum or plasma folate <3 ng/mL (microbiological assay)† Folate deficiency Red blood cell folate <140 ng/mL (microbiological assay) † *Senti FR, Pilch SM. Analysis of the folate nutritional status of the U.S. population based on data collected in the Second National Health and Nutrition Examination Survey, 1976–1980. Bethesda (MD): Federation of American Societies for Experimental Biology; 1984. † Due to lack of assay standardization, cut-off values may have to be adjusted depending on the assay used; 2 ng/mL for serum folate and 95 ng/mL for RBC folate were adjusted cut-off values used for the BioRad Quantaphase II assay. The BioRad Quantaphase II assay cut-offs will average approximately 30% less than cut-off values for the microbiological assay.