1
Supplemental Data
MANUSCRIPT TITLE:
Environmental concentrations and bioaccumulations of
cadmium and zinc in coastal watersheds along the Chinese
Northern Bohai and Yellow Seas
AUTHORS:
Wei Luo, Yonglong Lu, Tieyu Wang, Wentao Jiao, Wenyou Hu,
Jonathan E. Naile, Jong Seong Khim, John P. Giesy
ADDRESS:
State Key Lab of Urban and Regional Ecology, Research Center for
Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing
100085, China
Department of Veterinary Biomedical Sciences and Toxicology
Centre, University of Saskatchewan, Canada
NO. OF FIGURES:
0
NO. OF TABLES:
3
NO. OF PAGES:
4
2
Table S1. Sample details of all sampling sites along the Chinese Northern Bohai and Yellow Seas (CNBYS)
Sampling
Sub-sample number
Latitude
(º)
118.19920
Water a
Sediment a
Crab b
Carp b
TS1
Longitude
(º)
39.51171
5
5
0
4
Qionglong River
TS2
39.26820
118.52379
5
5
6
0
Bohai Sea
TS3
39.14441
118.33938
5
5
7
0
Shuanglong River
TS4
39.19876
118.33888
5
5
0
0
Bohai Sea
TS5
39.41793
119.27580
5
5
5
0
Luan River
TS6
39.46074
119.13412
5
5
5
0
Luan River
TS7
39.58211
118.80655
5
5
0
0
Bohai Sea
QH1
39.70235
119.33615
5
5
4
6
Xin River
QH2
39.84100
119.51119
5
5
5
8
Xin River
QH3
39.83960
119.51538
5
5
8
3
Bohai Sea
QH4
39.83131
119.52674
5
5
3
4
Tianma River
QH5
39.98022
119.21003
5
5
5
4
Bohai Sea
HL1
40.19749
120.37373
5
5
3
0
Liugu River
HL2
40.25276
120.35999
5
5
4
0
Liugu River
HL3
40.36981
120.25814
5
5
7
3
Wuli River
HL4
40.72748
120.89366
5
5
3
0
Bohai Sea
HL5
40.69957
120.94760
5
5
6
5
Daling River
JZ2
41.08476
121.13304
5
5
0
5
Daling River
JZ3
41.39898
121.41084
5
5
0
6
Daling River
JZ4
41.17702
121.37771
5
5
8
0
Bohai Sea
JZ5
40.90400
121.73938
5
5
5
0
Shuangtaizi River
PJ1
41.09955
121.59080
5
5
3
6
Bohai Sea
PJ2
40.81268
121.98571
5
5
4
0
Daliao River
YK1
40.82095
122.13346
5
5
5
0
Bohai Sea
YK2
40.66532
122.16431
5
5
5
5
Bohai Sea
YK3
40.42683
122.27681
5
5
3
4
Bohai Sea
DL1
39.61074
121.49876
5
5
8
0
Fuzhou River
DL2
39.69397
121.73738
5
5
0
8
Bohai Sea
DL4
38.99975
121.30857
5
5
0
0
Yellow Sea
DL3
38.87093
121.55736
5
0
0
6
Biliu River
DL5
39.46474
122.50038
5
5
5
0
Yellow Sea
DL6
39.65726
122.98590
5
5
4
7
Dayang River
DD1
39.94152
123.62830
5
5
0
0
Yalu River
DD2
40.17621
124.45491
5
5
0
0
Yellow Sea
DD3
39.94077
124.28782
5
5
0
5
Yalu River
DD4
40.00564
124.36262
5
5
0
0
Sea
Coastal region
River/Sea
Site
Northern Bohai Sea
Tangshan
Dou River
Qinhuangdao
(Liaodong Bay)
Huludao
Jinzhou
Panjin
Yingkou
Dalian
Northern Yellow Sea
Dandong
a
b
A composite sediment sample contained five collected sub-samples.
A composite sample of carp or crab contained three the same species individuals from the collected sub-samples.
3
Table S2. Mean concentrations and standard deviations for cadmium and zinc in water (μg/l), sediment (mg/kg,
dry weight) and biota (mg/kg, wet weight) in coastal watersheds along the CNBYS in previous studies
Sampling
Sample
Sampling location
Cd
Zn
Reference
size
River water
Dou River
7
6.2±9.6
—
[1]
River water
Luan River
6
1.8±1.3
—
[2]
Sea water
Daliao Estuary
3
0.15±0.080
39±5.3
[3]
Sea water
Yalu Estuary
6
0.43±0.30
—
[4]
Sea water
Bohai Sea
42
0.45±0.15
8.1±2.1
[5]
Sea water
Jinzhou Bay, Bohai Sea
90
1.9±0.65
28±19
[6]
Sea water
Liaodong Bay, Bohai Sea
160
1
31.5
[7]
Sea water
Northern Yellow Sea
74
0.19±0.05
6.0±1.8
[5]
River sediment
River sediment
River sediment
River sediment
Marine sediment
Marine sediment
Marine sediment
Marine sediment
Marine sediment
Marine sediment
Pomfret
Blue-spot sierra
Gobies
Wolf goby
Fish
Orithyia sinica
Hairy clam
Four angle clam
Hairy clam
Short necked clam
Four angle clam
Chinese clam
Mussel
Crustacean
Luan River
Wuli River
Liao River
Daliao River
Daliao Estuary
Yalu Estuary
Estuaries around Liaodong Bay
Northern Yellow Sea
Jinzhou Bay
Liaodong Bay
10
10
8
8
3
48
22
145
14
56
2
4
3
1
—
1
2
3
3
5
2
2
2
—
0.15±0.12
8±11
1.3±1.1
1.1±1.6
0.16±0.11
0.24±0.15
1.2±1.9
—
248.1±266.8
0.1±0.1
76±46
525±576
174.7±208.2
87.5±40.2
30±17
108.4±7.8
105.3±60.4
65.6±16.1
6419±5342.3
57.8±18.8
[8]
[9]
[10]
[11, 12]
[3]
[13]
[14]
[15]
[16]
[17]
0.28±0.20
0.28±0.23
0.45±0.15
0.88
0.26
1.21
0.59±0.0025
0.72±0.14
29.3±8.5
8.4±12.1
0.83±0.33
5.3±0.71
14±0.71
0.92
26.08±5.9
14.8±1.8
23.2±8.7
47.3
10.05
92.7
27.2±10.4
20.8±15.2
92.7±33
79.1±68.4
96.3±77.7
50.7±11.5
72±30.3
52
[18]
[18]
[18]
[19]
[20]
[19]
[21]
[21]
[18]
[18]
[18]
[18]
[18]
[20]
“
Sea near Huludao
Sea near Huludao
Sea near Huludao
Liao River Estuary
Yellow Sea
Liao River Estuary
Jinzhou Bay
Jinzhou Bay
Sea near Huludao
Sea near Huludao
Sea near Huludao
Sea near Huludao
Sea near Huludao
Yellow Sea
—” indicates that data is not available.
4
Table S3. Guidelines for water, sediment and biota
Guidelines
Cd
Zn
Referernce
Chinese environmental quality standard for surface water-Class I (μg/l)
1
50
[22]
Chinese environmental quality standard for surface water-Class II (μg/l)
5
100
[22]
Chinese sea water quality standard- Class I (μg/l)
1
20
[23]
Chinese sea water quality standard-Class II (μg/l)
5
50
[23]
Sediment background for Chinese rivers (mg/kg, dw)
0.13
68
[24]
Sediment background for the Northern Bohai and Yellow Seas (mg/kg, dw)
0.069
57
[25]
Chinese marine sediment quality-Class I (mg/kg, dw)
0.5
150
[26]
Sediment threshold effect level (TEL) (mg/kg, dw)
0.6
123
[27]
Sediment probable effect level (PEL) (mg/kg, dw)
3.53
315
[27]
Sediment effect range low (ERL) (mg/kg, dw)
5
120
[27]
Sediment effect range median (ERM) (mg/kg, dw)
9
410
[27]
Limit standard for the toxic substance in fishes (mg/kg, ww)
0.1
40
[28]
Limit standard for the toxic substance in crustaceans (mg/kg, ww)
0.50
150
[28]
5
REFERENCES
[1] Gong X. 2009. Water quality monitoring and pollution source analying of Dou River in city (in Chinese)
Journal of Hebei PolytechnicUniversity 31:132-136.
[2] Li Y, liu J. 2009. Health Risk Assessment on Heavy Metai Pollution in the Water Environment of Luan
River. Journal of Agro-Environment Science 28:1177-1184 (in Chinese).
[3] Liu J, Sun Q, Mo C, Guo N. 2008. The Pollution Status and Characteristics of Daliaohe Estuary and its
Adjacent Sea Area. Fisheries Science 27:286-289 (in Chinese).
[4] Liang T. 2005. Water contmanination and prevention along the coastal area of Dandong. Liaoning Urban
and Rural Environmental Science & Technology 25:21-23(in Chinese).
[5] TIAN L, Chen H, DU J, WANG X. 2009. Factors influencing distribution of soluble heavy metals in North
Yellow Sea surface seawaters. Periodical of Ocean University of China 39:617-621.
[6] Wan L, Wang N, Li Q, Sun B, Zhou Z, Xue K, Ma Z, Tian J, Song L. 2008. Distribution of dissolved metals
in seawater of Jinzhou Bay, China. Environ Toxicol Chem 27:43-48.
[7] Wan L, Wang NB, Li QB, Zhou ZC, Sun B, Xue K, Ma ZQ, Tian J, Du N. 2008. Estival distribution of
dissolved metal concentrations in liaodong bay. Bulletin of Environmental Contamination and Toxicology
80:311-314.
[8] Liu J, Li Y, Zhang B, Cao J, Cao Z, Domagalski J. 2009. Ecological risk of heavy metals in sediments of the
Luan River source water. Ecotoxicology 18:748-758.
[9] Zheng N, Wang Q, Liang Z, Zheng D. 2008. Characterization of heavy metal concentrations in the sediments
of three freshwater rivers in Huludao City, Northeast China. Environ Pollut 154:135-142.
[10] Shi J, Yu W, liu Z, Wang D. 1995. Heavy metal pollution and their potential ecological hazards in
Shuangtaizi River. Irrigation Works and Fishery:21-24 (in Chinese).
[11] Fan Y, Lin C, He M, Yang Z. 2008. Transport and Bioavailability of Cu , Pb , Zn and Ni in Surface
Sediments of Daliao River Watersystem. Environmental Science 29:3469-3476 (in Chinese).
[12] Fan Y, Lin C, He M, Yang Z. 2008. Kinetics and bioavailability of Cd in the surface sediments of the Daliao
River watersystem. Acta Scientiae Circumstantiae 28:2583-2589 (in Chinese).
[13] Gao J, Li J, Wang Z, Wang Y, Bai F, Cheng Y. 2008. Heavy metal distribution and their influence factors in
sediments of Yalu River Estuary and its adjacent Sea area. Geochim 37:430-438 (in Chinese).
[14] Zhou X, Wang E, Liu X, Wang W. 2004. Environmental geochemistry of heavy metals in bottom sediments
of the river mouths in Liaodong Bay. Geochimica 33:286-290 (in Chinese).
[15] Zou J, Shi X, Li S. 2007. Distributions of minor elements in near surface sediments in North Yellow Sea and
the early diagenesis. Marine Geology & Quaternary Geology 27:43-50 (in Chinese).
[16] Zhang Y, Wang L, Huo C, Guan D. 2008. Assessment on heavy metals pollution in surface sediments in
Jinzhou Bay. Mar Environ Sci 27:178-181(in Chinese).
[17] Hu N, Shi X, Huang P, Liu JH. 2010. Distribution of metals in surface sediments of Liaodong Bay, Bohai
Sea. China Environmental Science 30:380-388 (in Chinese).
[18] Yue L, Shi B. 2001. Monitoring the contaminated situation of the aquatic animals caused by heavy metals in
the inshore maritime area of Hulu island. Environmental Monitoring in China 17:45-47 (in Chinese).
[19] Ma MH, Hai Z, Feng Z, Guan C, Chen H. 1999. Study on the contents, temporal and spatial trends of the
contaminants in the marine animals along the coastal area near the mouth of Shuangtaihe river Liaodong Bay.
Marine Environmental Science 18:61-64,76 (in Chinese).
[20] Wei X, Tong J. 1993. The Pollution State of Huanghai (Yellow Sea) and Bohai Seas and in Ecolgical Impact.
Journal of Oceanography of Huanghai and Bohai Seas 11:76-82 (in Chinese).
[21] Wan L, Wang N, Ding Q, Zhang Q, Yu B. 2009. The distribution of heavy metal pollution in Jinzhou Bay,
Bohai Sea. Fisheries Science 28:801-804 (in Chinese).
[22] SEPAC (State Environment Protection Administration of China). 2002. Environmental quality standard for
surface water (GB 3838-2002) (in Chinese).
[23] SEPAC (State Environment Protection Administration of China). 1997. Sea water quality standard (GB
3097-1997) (in Chinese). Vol GB 3097-1997.
[24] Ma L, Yang X, Tong C, Wu A, Liu M. 2008. The geochemical characteristics of heavy metal elements in
sediments of Hunhe drainage area in Liaoning Province Rock Miner Anal 27:184-188 (in Chinese).
[25] Luo W, Lu Y, Wang T, Hu W, Jiao W, Naile JE, Khim JS, Giesy JP. 2010. Ecological risk assessment of
arsenic and metals in sediments of coastal areas of northern Bohai and Yellow Seas, China. Ambio 39:367-375.
[26] CSBTS(China State Bureau of Quality and Technical Supervision). 2002. National Standards GB
18668-2002-Marine Sediment Quality (GB 18668-2002). The People's Republic of China, p. 10 (in Chinese).
[27] MacDonald DD, Ingersoll CG, Berger TA. 2000. Development and evaluation of consensus-based Sediment
Quality Guidelines for freshwater ecosystems. Arch Environ Contam Toxicol 39:20-31.
5
6
[28] Ministry of Agriculture PRC. 2006. The limit standard for the toxic substance in the non-environmental
pollution aquatic products (NY 5073-2006) (in Chinese).
6