單
位：農學院 獸醫學系
教師姓名：蘇耀期
現
職：副教授
學
歷：台灣大學獸醫學 博士
Name：Su, Yao-Chi
E-mail：shu@mail.ncyu.edu.tw
TEL： 05-2717581
FAX： 05-2717566
簡要經歷：
1977 年陸軍行政少尉退伍(預官役)。
1977-1979 年任職美國雅培藥廠動物部業物代
表。
1981 年公務人員高等考試暨專門職業技術人員
高等考試獸醫類組及格。
1983-1986 年任職南投縣政府家畜疾病防治所
技士。
1986-1987 年任職省農林廳畜牧科技士。
1985-1987 年兼任國立嘉義農專獸醫科講師。
1987-1989 年任職國立嘉義農專畜產科專任講
師。
1989-1999 年任國立嘉義農專及嘉義技術學院
畜產系專副教授。
1991-1994 年兼任國立嘉義農專進修部教務組
組長。
1999 年奉 轉任國立嘉義大學農學院獸醫學系
專任副教授迄今。
研究興趣(專長)：
本人研究專長在 家畜寄生蟲病學、動物寄生蟲分類學、公衛衛生學及 臨床疾病診療等
1997~2002 年研究之方向：
台灣地區野鳥內寄生蟲之研究—建立資料庫。
台灣地區流浪犬貓人畜共通內寄生蟲之研究。
小動物疾病之研究：犬隻早期絕育對骨骼發育及生理之影響。
家禽球蟲之研究：發展以聚合脢鏈鎖反應偵測雞艾美屬球蟲。
2003-2012 年研究之方向：:
動物寄生蟲(各種動物、家畜與寵物)
草食動物疾病(牛、羊、鹿等)-牛羊鹿結核病、副結核病、乳房炎、披依菌等
主要教授課程：
獸醫寄生蟲學、獸醫原蟲學、臨床寄生蟲學、獸醫公共衛生學、診療實習二、人畜共通疾
病
2003~2010 主持及參與的研究計畫案：
1.寵物及野生動物疾病防治計畫 (92-管理-1.2-動防-01)
2.以基因引子鑑定球蟲之診斷開發計畫(92 農科-1.7.2-檢-B1(1))
3. 九十三年度流浪狗全國數目調查計畫(93-管理-1.4-動防-02)
4. 台灣中南部流浪犬猫人畜共通寄生蟲流行之研究計畫(93-管理-1.4-動防-05)
5. 寵物及野生動物疾病防治計畫(93-管理-1.1-動防-01)
6. 建立嘉義地區野生動物緊集醫療體系暨沿海棲地監護推廣計畫(93 農管-3.13-林-01)
7. 嘉義縣鰲鼓濕地環境現況調查—兩爬類 (93-嘉義縣政府)
8. 八掌溪河系河川情勢調查(1/2) (93-經濟部水利暑第五河川局與本校土木系合作計畫)
9. 鹿隻結核核病壓力素之研究(I) (94-農科-13.2.1-檢-B1)
10. 強化流浪犬處理計畫--動物收容所管理暨鳥類人道保定訓練計畫(94 管理-1.5-動防-02)
11. 重要境外動物疫病之預警及管制(1) (94-救治調整-檢-02(01))
12. 八掌溪河系河川情勢調查計畫(2/2)( 94-經濟部水利暑第五河川局與本校土木系合作計
畫)
13. 將軍溪河口濕地環境生態調查( 農委會林務局 95-00-5-15 與本校森林系合作計畫)
14. 鹿隻結核核病壓力素之研究(II)( 95 農科-13.2.1-檢-B1(1))
15. 貓隻早期絕育對生理行為及性腺激素之影響(95 農科-13.2.1-檢-B2)
16. 應用細菌分離、聚合酶鏈鎖反應、分子檢測法對乳羊潛在性乳房炎病原細菌之監測
(96農科-14.2.1-檢-B2 (9)
17. 以即時單引子巢氏PCR快速診緞台灣鹿隻結核分枝桿菌流行之研究(96農科-14.2.1-檢
-B2 (1)
18. 乳羊生乳源性葡萄球菌屬菌種之快速鑑定、腸毒素及抗藥性基因之監測(97農科
19.
20.
21.
22.
23.
24.
-14.2.1-檢-B1 (8)
黑面毗鷺棲習地病原監測計畫。(農委會林務局計畫，與台南縣政府家畜防治所合作)
乳牛羊及人源性金黃色葡萄球菌之抗藥性、毒力基因型別監測、分析及防治之研究(98
農科-9.2.4-檢-B1 (16)
草食動物結核病清淨模式之建立(98農科-9.6.1-檢-B3 (Z)
草食動物結核病清淨模式之建立(99農科-9.6.1-檢-B3(14)
天然災害動物死亡處理與疫病控制應變整備計畫(98特別-1.2-動防-01)
乳牛及乳羊乳房炎之防治與研究 (99農科-9.2.2-檢-B1(18)
蘇耀期副教授：
2003~2010 期刊論文:
1. Su YC, Fei ACY, Tsai FM. 2003. Differential diagnosis of five avian Eimeria species by
polymerase chain reaction using primers derived from the internal transcribed
spacer1(ITS-1)sequence.. Vet. Parasitol. 117: 221-227. (SCI)
2.Shiu SY, Liao MH, Su YC, Fei ACY. 2004. Antibiotics with moderate percentages of
sensitive isolates during 1998-2001. A. Environ. Microbiol. 12: 57~64.
3. Su YC, Fei ACY. 2004. Endoparasites of the Crested Goshawk, Accipiter trivirgatus
formosae, from Taiwan, Republic of China. Comparative Parasitology. 71(2), 178~183.
(SCI)
4. M.R. Chao, C.H. Hsien, C.M.Yeh, S.J. Chou, Y.C. Su, C.Y. Yu. 2007. Assssing the
Prevalence of Salmonella enterica in Poutry Hatcheries by Using Hatched Eggshell
Membranes. Poutry Science 86:1651-1655 (SCI)
5. Chih-Hung Hsien, Chishih ChU,Yao-Chi Su, Cheng-Ming Wang, Chang-You Yu. 2008.
Analysis of Prevalence and Antimicrobial Resistance of Salmonella enterica in Poultry
Breeders and Hatchery. J.Agric.For.(NCYU).70-86
6. C.Y. Yu, C.C. Chu, S.J. Chou, M.R. Chai, C.M. Yeh, D.Y. Lo, Y.C. Su, Ym. Horng, B.C.
Weng, J.G. Tsay, and K.C. Huang. 2008. Comparison of the Association of Age with the
infection of Salmonella and Salmonella enterica Serovar Typhimurium in pekin Ducks and
Roman Geese. Poutry Science 87:1544-1549 (SCI)
7. Ruei-Han Yeh，Tsai-Tzu Chen, Yao-Chi Su, and Kuo-Lung Chen. 2008. Dietary β
-1,3-1,6-glucan supplementation against Eimeria infection in broilers. J.Chin.Anim.Sci. 37(2):79~87
8. 邱敏蕙、余章游、蘇耀期、賴治民。2009。篩選具益生菌之鵝源乳酸桿菌。台灣獸醫學
雜誌。35(4): 253-259
9. Chishih CHU, Jyh-Mirn LAI, Chang-You YU, Wing-Shin CHANG, Hsing-Hong LIN, 1*
Yao-Chi SU . 2010. Rapid Identification of Staphylococcal Infection in Dairy Goat Raw Milk
by Multiplex PCR Assay. J.Taiwan Vet.36(3) ??
英文版
Photo
Name
School
Specialty
E-mail
Veterinary
Parasitology
Ph.D.,
Graduate
Institute
of
Dr. Su, Associated
Veterinary
Yao-Chi professor
Medicine,
and Practice,
Veterinary
Protozoology,
Veterinary
Public Health shu@mail.ncyu.edu.tw
and Practice,
National Practice of
Clinic 2,Clinic
Taiwan
University Veterinary
Parasitology,
Zoonosis
Su, Yao-Chi
Associate Professor
Professional specialty: Parasites in domestic and wild animals, Veterinary Public Health , Mastitis in dairy
cows and goats
Courses Taught:
Undergraduate:, Parasites in domestic and wild animals, Veterinary Public Health , Zoonoses, Clinical
Practice 2 , Disease of economic animal
Graduate: Advanced topics of Parasites in domestic and wild animals, Zoonoses
Tel: +886-5-2717581
E-mail: shu@mail.ncyu.edu.tw
Educational Background
Ph.D., Graduate Institute of Veterinary Medicine, National Taiwan University (2003)
BS, Graduate Institute of Veterinary Medicine, National Taiwan University (1985)
Current Position and Professional Career
Associate professor, Department of Veterinary Medicine, National Chiayi University (1990- )
Chief of the Public Health, Parasite, Protozoa in Veterinary Medical, National Chiayi University
(2000- )
Recent result of research
Rapid Identification of Staphylococcal Infection in Dairy Goat Raw Milk by Multiplex PCR
Assay. 2008.
To improvethe diagnosis accuracy of staphylococcal infection in dairy goat with
mastitis, we develops a multiplex polymerase chain reaction (PCR) assay by targeting
specific regions of staphylococcal 16S rRNA for identification of staphylococcal infection,
using specific nuc gene of S. aureus to distinguish S. aureus and the coagulase negative
Staphylococci (CNS), and mecA gene responsible for oxacillin or methicillin resistance to
determine methicillin resistant staphylococcus aureus (MRSA). The Application
Program Interface (API) method, and PCR method were used to examine 3,427 milk
samples from subclinically infected herds with traditional culture, and revealed 41.4 %
bacterial infection. E. coli, coagulase-negative staphylococci and S. aureus were the
predominant bacteria and their prevalence differed among farms. Genotype A1 S. aureus
was clonally disseminated on most farms, except farms A, B, D, and E.
Genetic and antimicrobial susceptibility variations ofStaphylococcus aureus from dairy goat
raw milk 2008.
Eighty-six Staphylococcus aureus (S. aureus) isolates were collected from 17 dairy goat
farms and were characterized as having eight biotypes, four coa genotypes and four nuc
genotypes based on biochemical characteristics, coa genotyping, and nuc genotyping methods.
The results of these three methods differed. However, the most prevalent isolates were of
biotype 2, coa genotype 2, and nuc genotype A. The antimicrobial susceptibility to 16 tested
antimicrobials was such that over 50% of isolates resisted ampicillin, oxytetracycline,
penicillin, and tetracycline, whereas all were susceptible to methicillin, cephalothin,
cefuroxime, enrofloxacin and bacitracin, and exhibited lower resistance to cloxacillin and
sulfamethoxazole/trimethoprim. Nearly 80% of isolates were multi-drug resistance (MDR).
Gene tetM, rather than tetK, was mainly responsible for resistance to tetracycline. In
conclusion, MDR S. aureus and one major genotype type circulated among dairy goat farms.
Study of Isolation, Distribution, and Enterotoxin Genes in Staphylococcus spp. Isolated
from Dairy Goat Farms. 2009.
In this study, analyzed 98 staphylococci, which included 27 S. aureus, 31 S. lentus, 23 S.
epidermidis, 17 S. xylosus, and all of them were isolated from four dairy goat farms in central
and south Taiwan. Staphylococcal incidence were higher in milk, vagina, anus, and nasal
cavity than in other isolation sources, which suggested that Staphylococcus spp. may survive
in dairy goat farms extensively. Enterotoxin genes sea~see and seg~sej were examined by
PCR amplification. The genes sec and see were found in 25 isolates and 1 isolate from 98
clinical staphylococcal strains collected in this study and 86 S. aureus previouly isolated in
laboratory. In addition, no enterotoxin gene was detected in any CNS isolate. In the result of
RFLP (restriction fragment length polymorphism) analysis, it was demonstrated that all sec
genes were identical in AluI digested pattern. Among 10 S. aureus isolated from goat mastitis,
8 S. aureus consisted of sec, which suggested that sec of S. aureus may be important
virulence factor for goat mastitis.
Mycobacterium tuberculosis and M. bovis Infection in Feedlot Cervus unicolor swinhoei and C.
Nippon taioumus in Taiwan
Mycobacterium bovis frequently infect and cause tuberculosis in wild and farm deer species.
Mycobacterial infection in two native deer species Cervus unicolor swinhoei (Formosan Sambar, Sambar) and
Cervus nippon taiouanu (Formasan Sika, Sika) was investigated. Among 12 ITT-testet Cervus unicolor swinhoei
(Sambar), M. bovis and two unknown mycobacterial isolates were identified. PCR amplification of
blood samples showed highest sensitivity to ITT results. Duplex PCR could differentiate MTBC (M.
bovis and M. tuberculosis) and M. avium. In contrast, multiplex nested PCR (mnPCR) assays
could differentiate M. bovis and M. tuberculosis. Therefore, we used blood samples and duplex
PCR and mnPCR methods to investigate the prevalence of mycobacterial infection in 167 Sambar deer
and 147 Sika deer. In contrast to no M. avium, M. bovis and M. tuberculosis were isolated in feedlot Sambar
and Sika by mnPCR amplification from blood samples. Prevalence of both species differed between deer species and
between regions for Sambar. Sequence variations
showed diverse sources of both mycobacterial species.
Emergence of Methicillin-Resistant and Genotyping Staphylococcus aureus Isolate from
Daily Goats. 2010.
Misuse of antimicrobial drug increases the emergence of multi-drug resistant
staphylococci, which become public health problem. We analyzed 58 staphylococci (27
Staphylococcus aureus isolates, and 31 Staphylococcus lentus isolates) collected from four
dairy goat herds in central and south area of Taiwan. Antibiotic susceptibility test showed that
Staphylococcus spp. had serious resistance to penicillin G, ampicillin, tetracycline, and
oxytetracycline, and most isolates were multidrug-resistant strains. PCR detection of
methicillin resistant genes of S. aureus and S. lentus found that 9 MRSA strains consisted of
SCCmec III, and 2 MRSA strains contained SCCmec II. By pulsed-field gel electrophoresis
(PFGE) analysis of MRSA collected from goat and human identical PFGE pattern A (5
isolates), C (1 isolate), and D (1 isolate) were found. In addition, two isolates belonged to
ST59. These results indicate that S. aureus might transmit between goat and human.
Study on Biology and Epidemiology of Chlamydophila abortus from goat in Taiwan.2010
In this study, a total of 224 vaginal swab samples from 9 farms in the mid-southern part of
Taiwan were collected from the year 2007 to 2009. These samples were categorized into two group
based on the history of abortion in does in these farms. Polymerase chain reaction (PCR) method
was used to test the existence of Cp. abortus, resulting in 10.8 % (13/120) of positive rate in
samples collected from farms with history of abortion and 0 % from farms without history of
abortion. Comparing samples collected from farms with abortion cases during the previous year and
previous week, we found that the positive rates showed 4.1 % (3/74) and 30.8 % (4/13) respectively,
indicating that sampling time might affect the results of the tests. In addition, PCR testing of
samples taken from does without abortion symptoms but were kept together with those
encountering abortion symptoms had a positive rate of 66.7 % (6/9), showing a high risk of
infection. Furthermore, nine aborted fetuses were also collected, two of which were detected
Cp.abortus positive by means of PCR and acid fast staining of tissue section. Based on the sizes of
aborted fetus and the time of abortion, we could generally see that the abortion of goats in Taiwan
occurred in two time period, 30 to 70 day and 90 to 120 day respectively. In serological testing, a
total of 409 serum from 19 farms were collected. Preliminary, choosing 30 serum samples from 6
farms in random and the seropositive rate was 60 % (18/30) using ImmunoComb® Ovine
Toxoplasma and Chlamydia antibody test kit. Later, the seropositive rate was 9.3 % (38/409) using
Cp. abortus-specific CHEKIT Chlamydophila abortus Antibody ELISA Test Kit.
Investigation of relationship between estrus physiology of sheep and shedding of Cp. abortus.
2010.
Of the 224 vaginal swab samples collected from the 9 farms from 2007 to 2009, and by using
chi-square statistic test , we found that there was a relationship between the amount of mucous on
the swabs and their Cp. abortus PCR testing results (P < 0.01). However, Cp. abortus detections of
animals in the latent infection stage were much more difficult to determine due to the minor
shedding of Cp. abortus. In accordance to the report that the amount of Cp. abortus shedding tent to
increase during estrus cycle, and to further corroborate it, 128 chosen does from 6 farms were
implanted with CIDR (control internal drug release, contain 366 mg progesterone) to collect their
vaginal mucous for the detection of Cp. abortus. In order to increase the sensitivity of the test,
nested PCR method was performed. Positive detection rate from farms with abortion history was
21.1 % (4/19) while farms without abortion history ranged from 5.4 % (2/37) to 16.7 % (3/18).
Total detection rate was 10.9 % (14/128). As for farms without abortion history, we compared the
PCR testing results between samples from CIDR source and non-CIDR source. We found that there
was a relationship between the mucous samples induced by CIDR and the PCR results (chi-square
statistic, P < 0.01). Based on this result, we could presume that, vaginal mucous samples collected
from does during CIDR induced estrus might elevate the early diagnosing of Cp. abortus in does
undergoing latent infection stage.
Selected Publications
1. Su YC, Fei ACY, Tsai FM. 2003. Differential diagnosis of five avian Eimeria species by
polymerase chain reaction using primers derived from the internal transcribed
spacer1(ITS-1)sequence.. Vet. Parasitol. 117: 221-227. (SCI)
2.Shiu SY, Liao MH, Su YC, Fei ACY. 2004. Antibiotics with moderate percentages of sensitive
isolates during 1998-2001. A. Environ. Microbiol. 12: 57~64.
3. Su YC, Fei ACY. 2004. Endoparasites of the Crested Goshawk, Accipiter trivirgatus formosae,
from Taiwan, Republic of China. Comparative Parasitology. 71(2), 178~183. (SCI)
4. M.R. Chao, C.H. Hsien, C.M.Yeh, S.J. Chou, Y.C. Su, C.Y. Yu. 2007. Assssing the
Prevalence of Salmonella enterica in Poutry Hatcheries by Using Hatched Eggshell
Membranes. Poutry Science 86:1651-1655 (SCI)
5.Chih-Hung Hsien, Chishih ChU,Yao-Chi Su, Cheng-Ming Wang, Chang-You Yu. 2008.
Analysis of Prevalence and Antimicrobial Resistance of Salmonella enterica in Poultry
Breeders and Hatchery. J.Agric.For.(NCYU).70-86
6..C.Y. Yu, C.C. Chu, S.J. Chou, M.R. Chai, C.M. Yeh, D.Y. Lo, Y.C. Su, Ym. Horng, B.C.
Weng, J.G. Tsay, and K.C. Huang. 2008. Comparison of the Association of Age with the
infection of Salmonella and Salmonella enterica Serovar Typhimurium in pekin Ducks and
Roman Geese. Poutry Science 87:1544-1549 (SCI)
7. Ruei-Han Yeh，Tsai-Tzu Chen, Yao-Chi Su, and Kuo-Lung Chen. 2008. Dietary β
-1,3-1,6-glucan supplementation against Eimeria infection in broilers. J.Chin.Anim.Sci. 37(2):79~87
8. Min-Huei CIOU,Chang-You YU, Yao-Chi SU, Jyh-Min LAI. 2009. Screening of the Probiotic
Activity of Lactobacillus spp. from Geese. J.Taiwan Vet. 35(4): 253-259
9. Chishih CHU, Jyh-Mirn LAI, Chang-You YU, Wing-Shin CHANG, Hsing-Hong LIN, 1*
Yao-Chi SU . 2010. Rapid Identification of Staphylococcal Infection in Dairy Goat Raw Milk by
Multiplex PCR Assay. J.Taiwan Vet. 36 (3) editing.