UNIVERSITY OF WESTMINSTER
School of Biosciences
Control of Substances Hazardous to Health (COSHH)
***** APPENDIX 4 - CHEMICAL SAFETY *****
Sheet........... of............
Title of Experiment
Room/Laboratory
Site
Affinity chromatography of using HPA lectin
5.13-5.15, cold room 7
th
floor
NCS
1.
Brief description of work
Affinity chromatography of proteins on Helix pomatia agglutinin (HPA) lectin linked to agarose beads.
Essentially involves loading proteins onto beads, washing with Tris buffer and eluting with Tris buffer containing 0.25M N-acetylglucosamine.
Note that a COSHH assessment for class activity may not be of a sufficiently high level to cover technician preparation; double assessment usually
required. Note also that it is RISK that is to be assessed, not HAZARD.
2.
LIST OF SUBSTANCES USED OR CREATED (continue on a separate sheet if necessary)
NAME OF SUBSTANCE
(CODE)
PHYSICAL
DESCRIPTION
MAX.
QUANTITY
EXPOSED
TO
g
DATA AVAILABLE
(YES/NO)
Tris base
powder
N-acetylglucosamine
Powder
Liquid
G
ml
Y
Hydrochloric acid
HPA lectin linked to agarose beads
Solid in liquid
ml
Y
y
Y
NOTE: 1) EXPLOSIVE
2) OXIDIZING 3) FLAMMABLE 4) TOXIC 5) HARMFUL 6) CORROSIVE
7) IRRITANT 8) CARCINOGEN 9) MUTAGEN 10) TERATOGEN 11) DUST 12) INHALATION
13) INGESTION 14) SKIN ABSORPTION 15) SKIN OR EYE 16) INJECTION 17) MAXIMUM EXPOSURE LIMIT
18) OCCUPATIONAL EXPOSURE STANDARD 19) RADIATION 20) OTHER (specify)
3.
KNOWN
HAZARDS
(See Note;
use numbers)
7 (irritant)
hygroscopic
Not available
4 (toxic)
6 (corrosive)
causes burns
No hazard data
available for HPA.
Ref Note 17, 18: see EH40 (current edition).
PERSONNEL INVOLVED WITH SUBSTANCES (continue on a separate sheet if necessary)
NAME/CLASS
N
Student
INVOLVEMENT - DATE OF USE or
DAILY/WEEKLY/MONTHLY/OCCASIONALLY
Occasionally, but at times in course of project work, daily
Panchar
Abbasi
S
Student
Occasionally, but at times in course of project work, daily
Dwek
MV
Supervisor /
Lecturer
Occassionally
4.
INITS
STATUS
OTHER GROUPS/PERSONS WHO MAY HAVE ACCESS TO THE SUBSTANCES
(e.g. students, cleaners, maintenance staff, contractors, visitors, storekeepers, etc.):
Stores
State control measures (to prevent inappropriate persons/groups having access to the substances ):
5.
EMERGENCY PROCEDURES (as per Hazard Data Sheet).
if any of the substances or procedures identified above is likely to pose a special hazard in an emergency, then identify below action to be taken.
CODE
Tris base
GlcNAc
HCl
HPA lectin
SPILLAGE/UNCONTROLLED RELEASE
For 100g quantities: Wear respirator,
safety goggles, rubber gloves and boots.
Sweep up. Place in bag and hold for
waste disposal. Ventilate area and wash
site after pick up.
For 100g quantities: Wear respirator,
safety goggles, rubber gloves and boots.
Sweep up. Place in bag and hold for
waste disposal. Ventilate area and wash
site after pick up.
Wear respirator, safety goggles, rubber
gloves and boots. Absorb on sand and
place in closed containers for disposal.
Ventilate area and wash spill site after
material pick up is complete.
Absorb on tissue paper and place in
closed containers for disposal.
FIRE
Use water spray or carbon dioxide
Use water spray or carbon dioxide
The acid is non combustible.
The lectin is non combustible.
If personnel are affected (fumes, contamination, etc.) treatment to be adopted:
SPECIFY PER SUBSTANCE
(CODE AS PER HAZARD DATA SHEET)
Hydrochloric acid:
Fumes  vacate area to area clear of fumes rest and keep warm. If exposure has been great obtain medical attention.
Skin contact  wash the area thoroughly under running tap water. Remove contaminated clothing and wash before reuse.
Eye contact  eye wash bottle irrigate for at least 10 minutes and seek medical advice
In the case of significant contamination either of fumes or to skin and ALWAYS to eyes, seek medical advice.
Tris base:
Inhalation  remove to fresh air, fi not breathing, give artificial respiration. If breathing is difficult, medical practitioners should give oxygen
Skin contact  wash with soap and copious amounts of water.
Eye contact  eye wash bottle irrigate for at least 10 minutes and seek medical advice
In the case of significant contamination either of fumes or to skin and ALWAYS to eyes, seek medical advice.
6.
CONTROL MEASURES TO BE ADOPTED (NB. Consider the following points, the appropriate Biosciences Safety Code plus any other
necessary measures. SPECIFY PER SUBSTANCE AS NECESSARY FOR EACH OF THE ITEMS BELOW.)
Always use HCl in fume cupboard, wear gloves (see COSHH form attached). Always add acid to buffer in small volumes NOT buffer to acid.
1. Limiting exposure time of personnel - state maximum time
2. Partial enclosure with local exhaust ventilation
3. Local exhaust ventilation
4. Sufficient general ventilation
5. Personal protective equipment (goggles/gloves/clothing/mask/breathing apparatus/safety screen, etc.) SPECIFY
* 6. Safe disposal/storage of hazardous substances
* 7. Regular cleaning of the workplace
* 8. Adequate washing facilities
* = MANDATORY
STORAGE - SAFETY CONSIDERATIONS
HCl  locked toxic cabinet
HANDLING PRECAUTIONS
HCl, wear nitrile gloves and safety spectacles / goggles / lab coat. Avoid skin
contact. Always use HCl in an extractor hood or fume cupboard..
Tris base  shelf in lab
Wear nitrile gloves and safety spectacles / goggles / lab
coat. Avoid skin contact.
DISPOSAL PROCEDURES DURING AND AT END OF EXPERIMENT
Dispose of plasticware after autoclaving via laboratory waste route. Dispose of Tris-HCl solutions into drain with large volumes of water to wash away.
7.
REVIEW AND MONITORING OF CONTROL MEASURES
(Required checks, and their frequency, on the adequacy and maintenance of the control measures during the course of the experiment)
(NB. Biosciences Safety Code in respect of health surveillance needs. This form is not adequate if health surveillance is required.)
8.
OTHER RELEVANT INFORMATION (continue on a separate sheet if necessary)
(e.g. restriction of usage; transportation risks; labelling requirements; assessment review date, etc.)
9.
I certify that, to the best of my knowledge, the above precautions will adequately control the risks from the hazardous substances listed. I have brought the
assessment to the notice of the relevant technicians; academic staff; students; researchers (delete as necessary).
Name of Assessor:
Miriam Dwek
Signed:
Status of Assessor:
Lecturer
Date: