Table S1 - General oligonucleotide primers used to amplify four fragments of
Dermatophagoides farinae mitochondrial genome
Primer Namei
COX1_16F
COX1_1324R
COX1_25Fshort_T
COX1_1282R_T
COX1_220F
CO2_581R
COX1_705F_T
12S_ACRF_125F
16S_ACRF_1155R
12S_ACRF_134F
16S_ACRF_1132R_T
ND4_626F
ND5_675R
ND5_600R
CytB_412F
CytB_847R
CytB_419F
CytB_818R
i
5'-M13 tailii
M13FORW
M13REV
M13FORW
M13FORW
Primer sequence (no M13 tail)
TGANTWTTTTCHACWAAYCAYAA
CDGWRTAHCGDCGDGGTAT
TCHACWAAYCAYAARRAYA
CCWVYTARDCCTARRAARTGTTG
ATRATHGGNGGNTTYGGIAA
CCRCARATYTCTGARCAYTGWC
TTGATTTTTTGGTCATCCAGA
TCRRATNKYGTGCCAGCAGT
TWWWWAGATAARAWCCARTCTG
GTGCCAGCAGTYKCGGTYAT
YRCCGRYYTGAACTCAGH
YTWCCWAARGCHCATGTDGA
TNACHARDGTDGAAGAATGRAC
AADGGAADYTGDGCICTYTT
AGGNTAYGTTYTTCCITGRG
GGRATAGAWCGWARRATDGC
GTTYTTCCTTGRGGICAAAT
GCRAATARRAARTATCATTCWGGTT
PCRiii
CO1.p (F)
CO1.p (R)
CO1.n (F)
CO1.n (R)
CO1-2.p (F)
CO1-2.p,h (R)
CO1-2.h (F)iv
12S-16S.p (F)v
12S-16S.p (R)e
12S-16S.sh (F)e
12S-16S.sh (R)e
ND4-ND5.p,h (F)
ND4-ND5.p (R)
ND4-ND5.h (R)
COB.p (F)
COB.p (R)
COB.sh (F)
COB.sh (R)
Primers were designed using alignment of parasitiform and acariform taxa; they are expected to work in
these groups, but were tested only in acariform mites
ii
M13FORW=TGTAAAACGACGGCCAGT, M13REV = CAGGAAACAGCTATGACC; M13 tails were
not consistently added to all nested primers; primers with M13 tails were used in other experiments
iii
product of parent PCR (p) is used for subsequent nested (n), heminested (h), or subheminested (sh)
PCRs; (F) = forward primer, (R) = reverse primer
iv
works but may be optimized further
v
specific to Acariformes