Overview Nutritional indicators are used to screen, diagnose, and evaluate interventions in individuals. They are also used in populations to determine the gravity of nutritional problems, their location and causes, and to evaluate the impact of programs and policies. This module is broken down into the following sub modules: Biochemical Anthropometric Clinical Dietary Anthropometric The purpose of anthropometric assessment is to take measurements and relate those data to standards or reference data. There are a number of anthropometric measurements that can be taken but the main ones related to population assessment are weight and height or length. In addition to the tools that can be found in this toolkit detailed procedures for various anthropometric measurements can be found in the MGRS anthropometric training video and in the module, Measuring a Child's Growth, of the WHO child growth assessment course, available at www.who.int/childgrowth/training. Anthro Software for analyzing anthropometric data is available on the WHO website at http://www.who.int/childgrowth/software/anthro_pc_manual.pdf. Clinical Signs and Symptoms Micronutrient deficiencies are often hidden problems in the population, with only the most severe forms showing symptoms. Ideally micronutrient deficiencies should be assessed biochemically but in some circumstances this is not always feasible. One alternate method is the assessment of the clinical signs and symptoms produced by various specific deficiencies. Although clinical signs and symptoms often appear only in the most severe cases, resulting in a gross underestimate of the prevalence of deficiency, they can indicate whether or not a specific deficiency is a severe problem in a population. Assessment of clinical signs and symptoms is often inaccurate because it depends on the expertise of the survey workers carrying out the examination. Because many deficiencies are relatively rare, few health workers have substantial experience with recognizing their signs and physical manifestations and there is high inter-observer variability is high. In addition, many of the signs and symptoms produced by micronutrient deficiency may have many other causes unrelated to nutrition. In general an assessment of clinical signs in national surveys is not common but may still be a part of an assessment in refugee or displaced populations. In this module there is a presentation with pictures and descriptions of clinical signs and a list of useful references for those people wishing to incorporate a clinical assessment into their survey. Biochemical Biochemical indicators are critical in the assessment of micronutrient status. Because such indicators may change more rapidly after the implementation of a treatment or preventive intervention, their measurement may demonstrate effectiveness sooner than other indicators, such as anthropometry or clinical signs and symptoms. However, collection of the specimens necessary to test biochemical indicators is substantially more complex than interviewing household respondents or weighing and measuring children or adults. This module contains tools and references specially related to biochemical indicators and cut offs. Information on processing, shipping, labeling and storage can be found in the laboratory module. Dietary For large cross sectional surveys, food frequency or 24-hour food recall modules to assess micronutrient status are rarely included, but may include a modified food frequency questionnaire. If a country has a specific intervention to increase the intake of a particular micronutrient, there are certain questions that can be included in the survey to assess exposure to factors that enhance or inhibit intake and absorption. For example a country might have various programs to improve iron status in the population and they might want to assess factors that can affect iron absorption, such as tea and coffee consumption. If countries have fortification programs, questions related to the use of the fortified products are usually included in the data collection forms. For some surveys it is necessary to obtain a small sample of the food for laboratory testing. For countries that want to conduct a dietary assessment there are tools that can help to estimate intakes of vitamin A and iron and zinc. There are also guidelines for the collection of salt and bread for laboratory testing. In the Resources section there are various guidelines and manuals and references on dietary indicators.