This Form is a template to write your Biosafety SOPs.
YOU MUST CUSTOMIZE THIS FORM ACCORDING TO YOUR
INDIVIDUAL RESEARCH PROJECT!
Please note: by filling out and sending this form along with your application, you are
stating that all things listed on this form will be implemented in your laboratory.
SOP for Receipt, Storage, Use and Disposal of Human Materials
Adherence to Universal Precautions
By sending the below SOP you are ensuring: BBP training for all personnel is complete.
2) The Hep B vaccination was offered and either declined or accepted. 3) An exposure
control plan is available in the lab.
All work with human materials is covered under the OSHA Bloodborne Pathogen
Standard and requires strict adherence to “Universal Precautions”. All human materials
are treated as potentially infectious until such point as they are exposed to a process such
as heat or chemical treatment that is known to inactivate bloodborne pathogens. All
laboratory workers handling human materials have completed bloodborne pathogen
training and have been offered Hepatitis B vaccination. An exposure control plan is
available in the laboratory.
Required Personal Protective Equipment and Clothing
A barrier between potentially infectious materials and the worker will be achieved by the
laboratory worker wearing personal protective equipment and clothing. This includes:
-Eye protection (minimum of safety glasses with side shields),
-Disposable gloves (nitrile or latex),
-Disposable lab coat or gown (tyvek or other liquid-resistant fabric)
Personal protective equipment and clothing will not be worn outside of laboratory areas.
Gloves must be removed and hands washed prior to leaving the laboratory.
Receipt of Human Material Samples
Human and animal samples consisting of (fill in the type of samples. i.e. serum, plasma,
cell extracts and cell line extracts) will be obtained from (list the source the samples are
obtained) a commercial vendor or a collaborator.
Personal protective equipment and clothing will be put on. (elaborate exactly what PPE
will be used)
Upon receipt in the laboratory (room #), sample packages containing frozen samples will
be opened in a biological safety cabinet (BSC). Alternatively, a chemical fume hood may
be used for personnel protection. (list which of the two you will be using). Primary
containers will be removed. Working in the BSC, they will be placed in a labeled
secondary container and removed to the freezer (type, room #). During transport the
secondary container will serve as a barrier so that no PPE is required in the hallways. The
freezer is labeled with a universal biohazard sticker.
Personal protective equipment and clothing are removed and discarded in a labeled
biohazard container. Hands are thoroughly washed prior to leaving the laboratory.
Work With Human Materials
Personal protective equipment and clothing will be put on.
Secondary containers are removed from the freezer and placed inside a biological safety
cabinet (BSC). Alternatively, a chemical fume hood may be used for personnel
protection. Frozen samples will be aliquoted from primary containers.
(Name the type of human sample you will be using. i.e. Serum and plasma) will be
handled using Universal Precautions until the proteins have been extracted and
inactivated using (write out type of inactivating process. i.e. heat (60-90C), and treated
with DTT (denaturing agent) followed by Iodacetamide (alkylating agent).)At that point
the material may be handled at BSL-1.
Upon completion of work, personal protective equipment and clothing are removed and
discarded in a labeled biohazard container. Hands are thoroughly washed prior to leaving
the laboratory.
Decontamination of Work Areas and Equipment
All work areas and equipment will be decontaminated with a liquid chemical disinfectant
(fill out the type of disinfectant to be used. i.e. sodium hypochlorite (bleach), Wescodyne,
or 70% Ethanol.) The disinfectant will be applied liberally. If bleach is used, the
solution will be made fresh on a daily basis using 1 part bleach in 10 parts water. The
solution will be labeled as to contents and date of preparation. Equipment to be
decontaminated includes (list all equipment that needs to be disinfected. ANY equipment
that comes into contact with the human material will need to be disinfected.)
Waste Management
Per Massachusetts regulations:
All sharps will be placed in a leakproof, labeled sharps container regardless of
whether they are contaminated with biohazardous material. Care must be taken to not
overfill the container. Close the container when approximately 2/3 full and use a new
container. The PIs name and lab number will be written on the side of the sharp
container and will be dropped off during EH&S waste room hours.
All biohazardous liquids will be treated with sodium hypochlorite (bleach). One
part bleach will be added to 10 parts of liquid. The solution will be allowed to sit for at
least 30 minutes prior to sink disposal.
All solid non-sharp biohazardous materials will be placed in labeled containers
lined with biohazard bags. Containers will be closed when not in use. When
approximately 2/3 full, the container will be removed to the autoclave room (room #) and
bags will be removed from the container and autoclaved (x minutes at y temperature).
When cool, autoclaved bags will be bagged in a black bag. All personnel will attend
autoclave training and take the online autoclave training through the EH&S web site.