PSYC 301L Winter 06: Lab Write-Up #1 Body Weight Experiment Your rats will undergo two distinct feeding regimes. Before you came into their life, your rat was fed ad libitum (free-feeding phase)s for a least 10 days. This phase will end for each rat when its weight remains stable for five days. The “maximum weight” for each rat (the average weight during the 5 day stable period) is referred to as the rat’s 100% weight. Today you will calculated the 100% weight for your animal and then calculated 85% of that weight in order to obtain the rat’s maintenance weight (85% of its 100% weight). In the second phase (restricted-feeding phase) each rat will be fed 10g of food per day or the number of grams that the rat is under its 85% weight, whichever is greater, each day. You will begin this process today. For the purposes of this lab write-up, you will be given the weight data from one of the rats, so everyone will base their results and discussion section on the one group of data. In order to complete this assignment you will be given: 1. a “daily weight and feeding chart” that shows the weight of the rat for ten days. Here, dates have been replaced with day number. The rat was free-fed for the first 10 days, then, the restricted feeding phase began on day 11. For each day beginning with day 11, fill in the amount of food that should have been weighed and fed to the rat (7 pts). Do not forget to take into account whether or not the rat was fed in the operant chamber (you can tell the rat was “run” in the operant chamber if a “before” weight is listed). 2. an introduction describing the body weight experiment. (You may be surprised that we are calling this an experiment, but in fact the procedure that we used to control the body weight of the rat is similar to the way in which many of these types of experiments begin: We provide different phases of training, each for some period of time, then look for changes over time in some dependent variable, and relate these changes to the training phases). Complete the APA formatted lab write-up by doing the following: 1. Title page (APA format) (5pts) 2. Write up a methods section. Here you will be outlining in detail how this experiment was done as well as details about the animal and equipment used. Here you will need to follow the standard format as outlined in the APA manual (ie., sections to include: subject, apparatus, procedure) (10 pts). 3. Prepare Figure 1. Create a graph showing the body weight on the vertical axis and days (numbered 1-20) on the horizontal axis. On days that the rat was run, use the before weight and ignore the after weight. For the other days, use the after weight. Separate the figure into two panels with a dashed vertical line, corresponding to the two treatment phases. Label the two panels and indicate the rat’s 85% weight on the second panel with a dashed horizontal line (remember the format necessary for including a figure in an APA formatted paper. In the figure caption include the subject’s number, the variables manipulated and measured, and just enough about the procedure that the reader can grasp the basic ideas of the experiment (10 pts). 4. Write a results (10 pts) and discussion (8 pts) section for this experiment. Although your figure will effective reflect the results of the experiment, you must still need to communicate the results in writing. Since a statistical analysis will not be performed you will not be reporting such things as F values, t values, alpha level, etc. However, you will can report systematic trends in the data, and relate these trends to the two feeding regimes. Write in the past tense and use objective language. Do not list raw data, but include some actual values to make your description concrete. For example, you might want to include the starting weight, the 100% weight (when it was reached), the 85% weight (when it was reached), etc. Conclude this paper by providing discussion, which interprets the data in terms of what was said in the introduction. Try to connect back to the “experimental hypothesis”, which was stated in the last sentence of the introduction provided. (Hint: What have your observations of your rat’s behavior and health suggested about the adequacy of the feeding protocol?) In addition, limitations and or problems in the experiment and suggestions for future research should be included. 4. Submit your work stapled in the following order: a. Title Page (5 pts) b. Method (10 pts) c. Results (10 pts) d. Discussion (8 pts) e. Figure (10 pts) f. Weight and feeding chart with the amounts fed filled in (7 pts) Total = 50 pts BACKGROUND The Body Weight of a Rat on Two Feeding Regimens In behavioral research in which food is provided as reinforcement, it is customary to maintain animals in a state of food deprivations. The obvious rationale for this is that in order for food to serve as an effective reinforcer, the animal must be sufficiently motivated to consume the food. The deprivation level is typically achieved by allowing the animal free access to food for some period of time, and then gradually reducing the animal’s weight to 85% of the maximum weight obtained during ad libitum feeding (free-feeding). Although a 15% reduction in body weight may seem extreme, this is a rather moderate level of deprivation. Hineline (1986) noted that a comparable method of determining the maximum free-feeding weight of a human would be to provide “continuous and easy access to an open, well stocked refrigerator and not much else” (p. 124). The implication here is that animals maintained on an ad libitum diet are likely to be overweight. Hineline (1986) observed that while free-feeding, food related behaviors are replaced with other patterns of behavior. Since wild animals must work hard to secure food, they should do so only when motivation is somewhat high; therefore, they would probably self-regulate their weights to something well under the maximum free-feeding weight. The purpose of the present experiment was to investigate the effect of two eating regimes on the weight of the animal and to determine whether the feeding procedure produces an adequate motivational state for operant behavior training with food reward without endangering the animal’s health or welfare.