2010F Daily Operation Manual (06/01/2007)
I. Schematic diagram of 2010F FasTEM

FEG
Gun valve

EDS aperture
(to block hard X-ray
from the gun)
Condenser lens aperture
(Manually controlled)


Sample

Objective lens aperture (OL)
On diffraction plane

High contrast aperture
Image plane

Used for STEM

Intermediate lens aperture (IL)
= selected area aperture
High angle annular dark field
(HAADF) detector (EXT)
Z-contrast imaging
TEI
Transmitted electron imaging (TEI) – small
version of HAADF for both dark and bright field

Off-axis TV
detector (the box
at the bottom)
GIF-TV
(Gatan image
filter)

Slow scan
camera (SSC)
2010F Daily Operation Manual (06/01/2007)
II. Fill in Log book
III. Check list before operation
See FAQ #1
IV. Sample Loading
(Refer to the sample loading and unloading instructions)
V. Operation
1. Unlock the sample stage
“FasTEM” folder, run “Remote to server”, on the remote desktop there
should be a Server window.
1) Choose right holder you are using.
2) Click "Ok" if you have made a change. If you have not made
a change, click "Close".
3) Make sure your Y-Tilt reading is 0. If Y-tilt is not zero, click
the minimized menu bar as indicated in the picture. Input
password: admin Exchange sample go to different holder
first click OK go to the holder you are usingClick OK.
Make sure the Y-tilt reading is correct. Contact staff if it is still not right. (This step is important to
have right Y tilt readout.) Failure to follow this will result in damaging the double tilt holder or
worse.
3) Close the remote window (not just minimize the window)
2. Check IGP<40, then open “Gun Valve” (L1)
3. Find the sample
Go to “Low Mag” (R1) mode; turn the ball to move sample around
3. Sample height
(1) “Mag1” (R1); Use “Brightness” (L1) to focus beam into a point on your sample
(2) Use “select” (R1) to go X 10k
(3) Use “objective focus” (R1) to set DV (green screen) at 0
(4) Slowly rotate joystick until the diffraction pattern disappear and only the focused spot is seen.
(5) Use “Brightness” (L1) to spread the beam.
4. Alignment for illumination
Typical setting: 1) Spot size 1; 2) alpha = 3; 3) C2 aperture = 150 um.
(In the green screen:
1
-3 )
It means
spot size

2010F Daily Operation Manual (06/01/2007)
(1) C2 aperture alignment; X100k
 Use “Brightness” (L1) to focus beam into a point.
 Use Shift X (L1) Y (R1) to center
 Turn “Brightness” (L1) clockwise to spread the beam.
If it’s off-center, it needs align. Align the aperture use knobs on the condenser aperture.
(2) Gun tilt; X 100k
 Use “Brightness” (L1) to focus beam into a point.
 Use Shift X (L1) Y (R1) to center
 Turn “Brightness” (L1) clockwise to spread the beam to about 1 inch in diameter.
 Push “Anode Wobb” (L1) and observe the moving cross.
 Push “Gun” (R2) under “Deflector”; CRS (R2) should be off.
 Use DEF knob (R2) to center the cross.
 Turn off “Anode Wobb” (L1) and “Gun” (R2)
(3) Gun shift; X 100k
 Change spot size (L1) to 5.
 Use “Brightness” (L1) to focus beam into a point.
 Use Shift X (L1) Y (R1) to center it.
 Change spot size (L1) to 1.
 Focus beam.
 Push Deflector “Gun” (R2) and use shift knobs (R2) to center.
(4) Condenser stigmatism; X 600k, alpha = 3, C2 = 150 um.
Simple way: Spread the beam (CW) to about the screen size. Adjust condenser stigmatism (L1) so
that the spread beam is round.
Complex way:
 Align CL again.
 Turn brightness CCW to make
1 cm
bright spot
 Use “Bright tilt” (L1) DEF X and Y (L1) to center the bright spot respect to the hallo.
 Turn “Brightness” CCW until a diamond-like shape is seen.
 Push “Cond Stig” (L1) and use def X and Y to make
into
(5) Pivot point alignment; X 200k
 Check DV is 0.
 Focus beam to a spot.
 Move “Tilt” left (to X).
 Frequency to 5
 Amplitude to 5
 Push “tilt”
 Push “CRS”
 Use (mainly) shift X, (sometimes) DEF X to make two spots
into one.
 Move “Tilt” right (to Y) and repeat the procedure.
 Turn of all the buttons used and move tilt to 0 (center)
2010F Daily Operation Manual (06/01/2007)
(6) Rotation center alignment; X 100k
 Spread the beam slightly larger than the screen.
 Find a small feature in sample (e.g. hole or a particle), focus sample using focus knob (R1). If the
focus is too far away, you need to change sample height to focus it.
 Turn on TV monitor; Make sure on the right knob on the TV-BOT controller is turned CCW to the
end.
 Push TV BOT on the pad (between L1 and L2); Slowly turn the right knob on the TV-BOT
controller CW until you see a sudden increase in the intensity (should be around 8 -10 o’clock);
 Push wobbler “HT” (R1): sample begins to move.
 Push “bright tilt” (L1)
 Change DEF X (L1) and DEF Y (R1) to minimize the motion of the sample.
 Push TEM on the Pad between L1 and L2; Turn of HT wobble (R1).
(7) insert objective aperture; X 200k
 “DIFF” (R1)
 On the computer, “OL” tab;
o
o
o
o
o
Push 1
 Use “DIFF FOCUS” (R1) knob to focus the edge of the aperture.
Make the aperture edge sharp
 Click the aperture tab and use the arrow on keyboard to center aperture respect to the bright disk.
 Go back to “Mag1”.
(8) objective stigmatism; X 300k
 “Brightness” (L1) to spread beam to screen size.
 Push “Gif/peels” (between L1 and L2), it should automatically pop-up “Search View” window. To
stop the scan, click the window and press space.
 If the image is shown on TV, push back to TEM (between L1 and L2), hold “Alt” “Auto filter”
window and click search. Change to slow scan CCD then push “Gif/peels”.
 Process-Live-FFT : A new window will show rings. If the rings are not oval, use object focus (R1)
to see big oval rings.
 Push “obj stig” (L1) to make oval into circle (slow response)
 Use “obj focus” to make the ring bigger.
 repeat  and  until one dark ring is seen.
2010F Daily Operation Manual (06/01/2007)
5. Find sample and take Image
Exposure 2.0 sec is normal. Make sure binning is 1 and click Full CCD. You can check it by
click the tool icon indicated by the red circle.
To Save:
 Browse, select folder
.“Saving” windowicon- “Save numbered”
Build using string
next index 1000 (1100 for next ...)
Always save as Gatan format, then convert images into tiff or jpg later on
 Select pictures and push
(Ctrl-Y)
 Ctrl-shift- to close all.
 To convert into tiff format: File – batch convert
VI. To finish
 Push “TEM” (between L1 and L2)
 Mag X 40 K
 Spread the beam.
 Push “N” (left side of the screen),
 Push “Valve” off.
 On the computer, in the Fast Client Window, make sure XYZ and TX and TY are all zero. For
single-tilt users, ignore the valve of TY. For double tilt holders, make sure check the shaft
position before taking holders out!!!! If there is problem, contact staff.
Aperture –
OL, IL “out”
HT tab, leave at 200KeV.
 Wear gloves and unload sample.
2010F Daily Operation Manual (06/01/2007)
 Put the holder back to the instrument.
 If the instrument will be used within 3 hr, fill cold trap.
If not, put the coil heater in trap, place the two pins in socket
 Push ACD HEAT (L2)
To copy data, use to PC computer in 0022 computer room. In the desktop, you should be able to find a
shortcut to 2010F users. You can convert and analyze your data using DigitalMicrograph here.
I. Information:
1. Joystick
X tilt
Y tilt
Z height
Top (FasTEM)
left-right
up-down
rotation
Bottom (Standard)
left-right
foot pedal
rotation