Figure S1 Phylogenetic tree using neighbor-joining method based on the predicted 96 aa
residues encoded by luxS genes from Vibrio isolates and all the sequences (described in
text) obtained from published data. Sequences isolated from M. laxissima (JZ08ML), I.
strobilina (JZ08IS) and seawater (JZ08SW) in this study are in bold and the accession
number of each of these luxS sequences is listed after the isolate name. The numbers in []
indicates the numbers of sequences in Genbank that shared over 99% identity to the
sequence on the tree and these sequences are listed in supplementary material Table 1.
The novel luxS cluster is named Cluster MI. Bootstrap values > 50% is shown at nodes.
The scale indicates the number of amino acid substitutions per site.
Figure S2 Alignment of predicted amino acid sequence of luxS72 (HM117146) to
predicted LuxS amino acid sequences from V. parahaemolyticus 16 (ZP_05120871), V.
cholerae O1 (NP_230208), V. fischeri ES114 (YP_203928), and V. harveyi ATCC BAA1116 (YP_001446656). luxS of V. parahaemolyticus 16 was used as a reference. Dashes
indicate that the amino acids are the same as those of the reference sequence. The number
at the beginning of each line indicates the position of the first amino acid in the line.
Asterisks indicate the conserved amino acid residues recognized in Hilgers and Ludwig
(2001).
Figure S3 Rarefaction curve for the Vibrio luxS gene amino acid sequences from M.
laxissima (ML), I. strobilina (IS) and surrounding seawater (SW). Operational taxonomic
units were defined at distance of 0.01.