DEPARTMENT OF BIOLOGICAL SCIENCES
(BIOCHEMISTRY PROGRAMME)
COLLEGE OF SCIENCE & TECHNOLOGY
COVENANT UNIVERSITY
COURSE OUTLINE
COURSE CODE:
BCH 411
COURSE TITLE:
ADVANCED ENZYMOLOGY
UNITS:
2
SEMESTER/ SESSION:
ALPHA, 2014/2015
COURSE LECTURERS:
Dr. S.N. CHINEDU & Dr. A. H. ADEBAYO*
*Course Coordinator
A.
COURSE DESCRIPTION:
The course seeks to expand the scope of the subject of enzymology taught as BCH 312
in 300 Level. It begins with a review of the chemistry of enzyme catalysis and the
measurement of the rate of enzyme catalyzed reactions at the steady state. It also
discusses various methods of measuring the rates of very fast reactions. It also
discusses the phenomenon of allosteric control and allosterism and the characteristic
properties of multienzyme complexes. The methods of determining the activity of
enzymes and the criteria for determining the purity of enzymes will be discussed. The
course will also answer the question of how the activity and synthesis of enzymes are
regulated and will discuss some latest developments in the field of enzymology.
B.
AIMS:
To study enzyme kinetics, catalysis, and assays, and know how they are
regulated, and their current advances in enzymology.
C.
COURSE OBJECTIVES:
At the end of this course, the students should be able to:

Learn the various simple methods of enzyme assay

Understand enzyme kinetics and their methods.

Know the difference between regulatory and non regulatory enzymes and the
principles of allosteric control.

Recognize the chemistry and mechanisms of enzyme catalysis.

Have a deeper knowledge on multi-enzyme complexes, molecular model for
allosterism and regulatory enzymes.

Explore the various principles and techniques for determining the purity of
enzymes.

Know how enzymes are synthesized, characterized and their activity regulated.

Examine and understand recent development of enzymes and their applications
in food, medicine and industry.
D.
METHODS OF LECTURE DELIVERY /TEACHING AIDS
Lecture Delivery and Teaching Aids include
E.

Guided instructions

Interactive classroom session

Multimedia and transparences

Diagrams
COURSE OUTLINE
Module 1
Enzyme
kinetics
and
techniques
for
enzyme
kinetic
measurement- Dr. A.H. Adebayo
Week 1
Chemistry of Enzyme catalysis
Week 2
Steady state enzyme kinetics arising from Michaelis-Menten’s
equation and Lineweaver-Burk’s plot,
Week 3
Steady state enzyme kinetics from Briggs-Haldane hypothesis,
Hanes-Woolf’s plot, Eadie-Hofstee plot.
Week 4
Transient kinetic measurements
MODULE 2 Allosteric Enzymes, Allosterism and Multienzyme complexes –
Dr. S.N. Chinedu
Week 5
Regulatory enzymes and allosteric enzymes
Week 6
Molecular models for allosterism
Week 7
Multienzyme complexes and enzyme reconstitution
Mid-term Test
MODULE 3 Measurement of enzyme activity and enzyme purityDr. A.H. Adebayo
Week 8
Enzyme Assays
Week 9
Criteria for determining the purity of enzymes
MODULE 4 Enzyme Regulation and recent advances in EnzymologyDr. S.N. Chinedu
F.
Week 10
Regulation of Enzyme activity
Week 11
Regulation of Enzyme synthesis
Week 12
Applications of Enzymes and Recent Advances in Enzymology
Week 13
Tutorials
Week 14
Revision
STRUCTURE OF THE PROGRAMME/ METHOD OF GRADING
1.
2.
G.
Continuous Assessment
30 Marks
(i)
Practical test
5 Marks
(ii)
Test 1
5 Marks
(iii)
Mid-Semester Test
20 Marks
Semester Examination
70 Marks
CLASS BEHAVIOUR
Please note the following:
H.

Mandatory 75% class attendance,

No eating or dozing in the classroom or laboratory,

Active participation in all activities,

All class assignments must be submitted on time,

Punctuality to classes must be observed.
TOPICS FOR TERM PAPERS/ASSIGNMENTS
(i)
Explain the criteria for determining the purity of an enzyme
(ii)
Discuss the major mechanisms for enzymatic catalysis.
I.
(iii)
Using a named enzyme, describe the regulation of enzyme synthesis.
(iv)
Describe the various techniques involved in enzyme assay.
(v)
Give an explorative review on some recent developments in enzymology.
INDUSTRIAL RELEVANCE
Advance
Enzymology
will
find
relevance
in
food,
biomedical,
diagnostics,
biotechnological and agricultural industries.
J.
RECOMMENDED READING
1. Stryer, L. Biochemistry (4th edition).
2. Lehninger, A. Principles of Biochemistry (5th edition)
3. Cornish-Bowden. Fundamentals of Enzyme Kinetics.
4. Fersht. Enzyme structure and mechanism.
5. Suckling C. J. Enzyme Chemistry: Impacts and applications (2nd edition).