Immobilised enzymes
Immobilisation of enzymes
- the advantages.
- To immobilize something or someone means to
stop them from moving or operating.
- It permits re-use of the enzyme preperation
- The product is obtained enzyme free
- The enzyme may be much more stable and long
lasting
Entrapment of enzymes between fibres
The enzyme is not chemically attached
Silica gel lattice or
polysaccharide from
seaweed
Entrapment in permeable microcapsules.
The enzyme is held securely
Capsule wall
Covalent bonding of enzyme to matrix
Prevents enzyme fom being leached away
Matrix of
polymer such as
cellulose or
collagen
Directed crosslinking of enzyme molecules by
covalent bonding with bridging molecules
Glutaraldehyde
molecules forming the
crosslink
Adsorption to a surface, where it’s bonded with
bridging molecules
Ion-exchange
resin or clay
Production of lactose free milk
* example of immobilisation of enzymes
Column of
immobilised
lactase
Pasteaurisation
process
Quality
control
phase
Packaging
plant