UNIVERSITY OF WESTMINSTER
School of Biosciences
Control of Substances Hazardous to Health (COSHH)
***** APPENDIX 4 - CHEMICAL SAFETY *****
Sheet........... of............
Title of Experiment
Room/Laboratory
Site
Staining / developing dot blots and western blots
5.08, instrument lab 7
th
floor
NCS
1.
Brief description of work
Developing dot blots / Western blots with HPA lectin using enhanced chemiluminescence.
Note that a COSHH assessment for class activity may not be of a sufficiently high level to cover technician preparation; double assessment usually
required. Note also that it is RISK that is to be assessed, not HAZARD.
2.
LIST OF SUBSTANCES USED OR CREATED (continue on a separate sheet if necessary)
NAME OF SUBSTANCE
(CODE)
PHYSICAL
DESCRIPTION
MAX.
QUANTITY
EXPOSED
TO
Mg
DATA AVAILABLE
(YES/NO)
HPA lectin – biotinylated
Powder / liquid
Streptavidin – peroxidase
Powder / liquid
Liquid
Mg
Ml
N
ECL reagents – reagent 1
ECL reagents – reagent 2
Liquid
Microlitres
Y
Tris buffer pH 7.6
Liquid
100’s of ml
Y
Tween 20 detergent (polyoxyethylene
sorbitan monolaurate)
Liquid
100’s of
microlitres
y
DAB (3',3'-DIAMINOBENZIDINE)
Powder
G
Y
Hydrogen peroxide 30%v/v
Liquid
Ml
Y
Methanol
Liquid
Ml
Y
Acetic acid (glacial)
Liquid
Ml
y
Amino caproic acid
Solid
G
Y
n
y
NOTE: 1) EXPLOSIVE
2) OXIDIZING 3) FLAMMABLE 4) TOXIC 5) HARMFUL 6) CORROSIVE
7) IRRITANT 8) CARCINOGEN 9) MUTAGEN 10) TERATOGEN 11) DUST 12) INHALATION
13) INGESTION 14) SKIN ABSORPTION 15) SKIN OR EYE 16) INJECTION 17) MAXIMUM EXPOSURE LIMIT
18) OCCUPATIONAL EXPOSURE STANDARD 19) RADIATION 20) OTHER (specify)
3.
KNOWN
HAZARDS
(See Note;
use numbers)
Toxicity and hazard
data not available,
exercise due care
Ditto
5 (harmful)
6 (corrosive
4 (toxic)
7 (irritant)
15 (skin and eyes)
5 (harmful)
6 (corrosive)
4 (toxic)
7 (irritant)
15 (skin and eyes)
7 (irritant)
hygroscopic
15 (skin and eyes)
Classification: nonhazardous but
exercise due care
7 (irritant)
15 (skin and eyes)
8 (potential
carcinogen)
2 (oxidising)
6 (corrosive)
3 (flammable),
4 (toxic)
7 (irritant)
15 (skin and eyes)
3 (flammable)
5 (harmful)
6 (corrosive)
5 (harmful)
7 (irritant)
Ref Note 17, 18: see EH40 (current edition).
PERSONNEL INVOLVED WITH SUBSTANCES (continue on a separate sheet if necessary)
NAME/CLASS
INITS
STATUS
N
Student
INVOLVEMENT - DATE OF USE or
DAILY/WEEKLY/MONTHLY/OCCASIONALLY
Occasionally, but at times in course of project work, daily
Panchar
Abbasi
S
Student
Occasionally, but at times in course of project work, daily
Dwek
MV
Supervisor /
Lecturer
Occasionally
4.
OTHER GROUPS/PERSONS WHO MAY HAVE ACCESS TO THE SUBSTANCES
(e.g. students, cleaners, maintenance staff, contractors, visitors, storekeepers, etc.):
Stores
State control measures (to prevent inappropriate persons/groups having access to the substances ):
5.
EMERGENCY PROCEDURES (as per Hazard Data Sheet).
if any of the substances or procedures identified above is likely to pose a special hazard in an emergency, then identify below action to be taken.
SPILLAGE/UNCONTROLLED RELEASE
ECL reagents
FIRE
Use a water spray, carbon dioxide or dry chemical powder / foam.
Emits toxic fumes under fire conditions.
Tris buffer
Wear respirator, chemical safety goggles,
rubber boots and heavy rubber gloves. Sweep
up, place in bag and hold for waste disposal
Avoid raising dust and ventilate area and wash
spill site after material pick-up is complete.
Use a water spray, carbon dioxide or dry chemical powder / foam.
Emits toxic fumes under fire conditions.
Tween 20
Exercise appropriate precautions to
minimise the direct contact with skin or
eyes and prevent inhalation of vapours.
Use a water spray, carbon dioxide or dry chemical powder / foam.
Emits toxic fumes under fire conditions.
Tween 20
continued
Wear respirator, chemical safety goggles,
rubber boots and heavy rubber gloves. Absorb
on sand and place in closed containers for
disposal. Ventilate area and wash spill site
after material pick-up is complete
Wear respirator, chemical safety goggles,
rubber boots and heavy rubber gloves. Absorb
on sand and place in closed containers for
disposal. Ventilate area and wash spill site
after material pick-up is complete. Call for
assistance on disposal. Finish cleaning by
spreading water onto the contaminated surface.
Wear respirator, chemical safety goggles,
rubber boots and heavy rubber gloves. Absorb
on sand and place in closed containers for
disposal. Ventilate area and wash spill site
after material pick-up is complete
Wear respirator, chemical safety goggles,
rubber boots and heavy rubber gloves. Absorb
on sand and place in closed containers for
disposal. Ventilate area and wash spill site
after material pick-up is complete
Wear respirator, chemical safety goggles,
rubber boots and heavy rubber gloves. Absorb
on sand and place in closed containers for
disposal. Ventilate area and wash spill site
after material pick-up is complete
Wear respirator, chemical safety goggles,
rubber boots and heavy rubber gloves. Sweep
up, place in bag and hold for waste disposal
Avoid raising dust and ventilate area and wash
spill site after material pick-up is complete.
Ditto
DAB
H2O2
Methanol
Acetic acid
Amino
caproic acid
If personnel are affected (fumes, contamination, etc.) treatment to be adopted:
Use a water spray, carbon dioxide or dry chemical powder / foam.
Emits toxic fumes under fire conditions.
Use a water spray, carbon dioxide or dry chemical powder / foam.
Emits toxic fumes under fire conditions.
Use a water spray, carbon dioxide or dry chemical powder / foam.
Emits toxic fumes under fire conditions.
Vapour may travel considerable distance to source of ignition and
flash back.
Use a water spray, carbon dioxide or dry chemical powder / foam.
Emits toxic fumes under fire conditions.
Use a water spray, carbon dioxide or dry chemical powder / foam.
Emits toxic fumes under fire conditions.
SPECIFY PER SUBSTANCE
(CODE AS PER HAZARD DATA SHEET)
If swallowed, wash out mouth with water provided if person is conscious. Call a physician.
If inhaled remove to fresh air. If breathing becomes difficult call a physican. In case of contact immediately wash skin with soap and copious quantities of water. In
case of contact with eyes, flush with copious quantities of water for at least 15 minutes. Assure adequate flushing by separating eyelids with fingers. Call a
physician.
DAB:
In case of an accident or if you feel unwell, seek medical advice immediately. In case of contact with eyes rinse immediately with plenty of water and seek
medical advice.
(NB. Antidotes and special treatment may be obtained through...................................................................................................................................)
6.
CONTROL MEASURES TO BE ADOPTED (NB. Consider the following points, the appropriate Biosciences Safety Code plus any other
necessary measures. SPECIFY PER SUBSTANCE AS NECESSARY FOR EACH OF THE ITEMS BELOW.)
Always use HCl in fume cupboard, wear gloves (see COSHH form attached). Always add acid to buffer in small volumes NOT buffer to acid.
1. Limiting exposure time of personnel - state maximum time
2. Partial enclosure with local exhaust ventilation
3. Local exhaust ventilation
4. Sufficient general ventilation
5. Personal protective equipment (goggles/gloves/clothing/mask/breathing apparatus/safety screen, etc.) SPECIFY
* 6. Safe disposal/storage of hazardous substances
* 7. Regular cleaning of the workplace
* 8. Adequate washing facilities
* = MANDATORY
HANDLING PRECAUTIONS
STORAGE - SAFETY CONSIDERATIONS
HCl  locked toxic cabinet
Wear nitrile gloves and safety spectacles / goggles / lab coat. Avoid
skin contact. Always use HCl in an extractor hood or fume
cupboard..
DAB  freezer (-20’C)
Wear nitrile gloves and safety spectacles / goggles / lab coat. Avoid
skin contact.
Wear nitrile gloves and safety spectacles / goggles / lab coat. Avoid
skin contact.
Wear nitrile gloves and safety spectacles / goggles / lab coat. Avoid
skin contact.
Wear nitrile gloves and safety spectacles / goggles / lab coat. Avoid
skin contact. Always use in an extractor hood or fume cupboard..
Wear nitrile gloves and safety spectacles / goggles / lab coat. Avoid
skin contact.
Hydrogen peroxide  fridge
ECL reagents  fridge
Methanol  solvent storage cabinet
Tris, amino caproic acid  on shelf in lab.
DISPOSAL PROCEDURES DURING AND AT END OF EXPERIMENT
Dispose of plasticware after autoclaving via laboratory waste route. Dispose of Tris-HCl solutions into drain with large volumes of water to wash away.
DAB must be neutralised in bleach prior to disposal via chemical disposal officer. Solvent waste via the chemical disposal officer. ECL reagents via the chemical
disposal officer.
7.
REVIEW AND MONITORING OF CONTROL MEASURES
(Required checks, and their frequency, on the adequacy and maintenance of the control measures during the course of the experiment)
(NB. Biosciences Safety Code in respect of health surveillance needs. This form is not adequate if health surveillance is required.)
8.
OTHER RELEVANT INFORMATION (continue on a separate sheet if necessary)
(e.g. restriction of usage; transportation risks; labelling requirements; assessment review date, etc.)
9.
I certify that, to the best of my knowledge, the above precautions will adequately control the risks from the hazardous substances listed. I have brought the
assessment to the notice of the relevant technicians; academic staff; students; researchers (delete as necessary).
Name of Assessor:
Miriam Dwek
Signed:
Status of Assessor:
Lecturer
Date: