斑馬魚在基因體及醫學研究上的應用
EST Analysis of mRNAs Expressed in Gill and Skin of Zebrafish
蕭崇德
EST Analysis of mRNAs Expressed in Gill and Skin of Zebrafish
Chung-Der HSIAO, Yi-Er SHIEH, Yu-Chun WANG and Pung-Pung HWANG
Institute of Zoology, Academia Sinica, Taipei, Taiwan
cdhsiao@gate.sinica.edu.tw
Skin and gill are two major tissues responsible for osmoregulation in fish embryo
and adult, respectively. In order to reveal the molecular heterogeneity of mRNA
transcripts in gill and skin tissues, we constructed two un-normalized cDNA libraries
from adult zebrafish (Danio rerio) skin and gill tissues. Expressed Sequence Tags (ESTs)
constitute a rapid and informative strategy for studying gene-expression profiles of
specific tissues. We have collected up to 5000 ESTs from gill and skin libraries in the
present study. The gene redundancy for skin and gill libraries was 34 % and 52 %,
respectively. Based upon WU-BLASTN search against TIGR database, up to 25 % novel
ESTs were identified for the first time. The spatio-temporal expression pattern of selected
ESTs was evaluated by RT-PCR and whole-mount in situ hybridization in adult and
embryonic stages. This approach leads us to successfully identify several mRNA
transcripts preferentially expressed in skin or gill tissues. For example, ictacalcin was
identified as an excellent marker to follow the skin as well as pharynx formation during
zebrafish embryogenesis (Hsiao et al., 2003). Therefore, our data provides cues for
further studies on genes that are expressed in and function in the skin and gill tissues of
zebrafish.
Hsiao CD, Ekker M, Tsai HJ. 2003. Skin-specific expression of ictacalcin, a homolog of
the S100 genes, during zebrafish embryogenesis. Dev. Dyn. 228: 745-750.