The Effects of Stimulus Presentation on Anxiety Purpose The purpose of this experiment was to determine if there was a difference in anxiety between exposures to a real spider when compared to a picture of a spider. Methods Subjects Twenty-four college aged subjects with a history of arachnophobia were recruited. Data Collection The subjects were randomly assigned to one of the following groups: shown a picture of a spider, shown a real spider. After viewing either the picture or the real spider the subject’s anxiety was measured. Measurement of Reliability Prior to initiating the study, five subjects (not in the experiment), participated in a test-retest assessment of measurement reliability. Anxiety was measured on two separate days. Reproducibility of pull-up measurements was analyzed using SPSS (18.0 for Windows) to compute the intraclass correlation coefficient (ICC) using a two factor mixed effects model and type consistency (McGraw and Wong, 1996; Shrout and Fleiss, 1979). A high degree of reliability was found with the single measure ICC = .921 and the averaged measures ICC = .996. A Priori Statistical Power Analysis G*Power version 3.1.2 was used to determine sample size using a mean ± sd of 40 ± 6.9 for the picture group and a mean ± sd of 50 ± 6.9, a 2-tailed t- test and an alpha level of 0.05. To obtain an estimated power of 80%, 12 subjects per group would be required. Statistics All statistical analyses were performed using a commercial software package (SPSS version 18.0 for Windows, SPSS Inc, Chicago, IL). The anxiety for each group (picture, real spider) was analyzed to determine if the distributions were normal using Kolmogorov-Smirnov goodness-of-fit test and the Shapiro-Wilk Normality test. An independent t-test was used to compare the effects of the stimulus (picture, real spider) on anxiety. Alpha was set at p = 0.05. Data are expressed as mean ± SD. Results The effects of the stimulus presentation on anxiety are shown in Table 1. Tests of normality indicated that differences in anxiety between the two stimulus presentations was normally distributed. Levine’s test for equality of variance indicated that the groups had equal variance. As shown in Table 1, there was no difference in anxiety between the two methods of exposure to a spider [t(22) = −1.681, p = .107]. The stimulus had a medium effect size of 0.34 [Reference Cohen]. Table 1. Effects of stimulus presentation on anxiety (Mean ± SD) Anxiety (units?) * Picture of a Spider 40.00 ± 9.29 Real Spider 47.00 ± 11.03* p < 0.05; independent t-test. Conclusion Arachnophobia individuals experience the same level of anxiety when shown a real spider as they do when shown a picture of a spider. References Cohen J. Statistical Power Analysis for the Behavioral Sciences, Lawrence Erlbaum Associates: Hillsdale, NJ, 1988. McGraw KO and Wong SP. Forming inferences about some intraclass correlation coefficients. Psychological Methods 1: 30-46, 1996. Shrout PE and Fleiss JL. Intraclass correlations: Uses in assessing reliability. Psychol Bull 86: 420-428, 1979.