Curriculum Vitae
Bahman “Beau” Rostama
bahman.rostama@maine.edu
Work Experience
University of Maine Laboratory Rotation #4 (MMCRI, Scarborough, ME)
07/10 - present
Graduate Student
Principal Investigator: Lucy Liaw, Ph.D.
 Project: Characterization of changes in miRNA-221 and -222 in human aortic smooth muscle cells (hASMC)
upon Notch stimulation.
Techniques utilized: tissue culture of primary hASMC cells, RNA extraction, PCR, RT-qPCR, adenoviral
transduction of cells, light and fluorescent microscopy.
University of Maine Laboratory Rotation #3 (UNE, Biddeford, ME)
05/10 – 07/10
Graduate Student
Principal Investigator: David Mokler, Ph.D.
 Project: Determination of the hemispheric differences in presynaptic serotonin transporter protein (SLC6A4)
in the prefrontal cortices (PFCs) and striata of adult male Long-Evans rats.
Techniques utilized: dissection of rat brains, isolation of synaptosome fractions from tissues, BCA assay,
acrylamide gel electrophoresis, traditional and fluorescence-tagged western blotting, coomassie gel staining.
University of Maine Laboratory Rotation #2 (MMCRI, Scarborough, ME)
01/10 – 05/10
Graduate Student
Principal Investigator: Cliff Rosen, M.D.
 Project 1: Characterization of β-catenin translocation from cytoplasmic to nuclear compartments in preadipocyte and pre-osteoblastic, 3T3L1 and MC3T3 cell lines using modified sucrose fractionation techniques.
 Project 2: Analysis of the impact of Vitamin D receptor insufficiency on the ex-vivo induced differentiation of
osteocyte and adipocyte progenitors.
 Techniques utilized: RNA isolation, cDNA preparation, PCR, RT-qPCR, Superarray qPCR, agarose and
acrylamide gel electrophoresis, BCA assay, western blotting, subcellular compartment fractionation, light
microscopy, tissue culture of primary mouse calvarial osteoblast, marrow stromal, and peripheral adipocyte
cells, von kossa staining, alizarin red staining, alkaline phosphatase staining, TRAP staining.
University of Maine Laboratory Rotation #1 (MMCRI, Scarborough, ME)
09/09 – 12/09
Graduate Student
Principal Investigator: Igor Prudovsky, Ph.D.
 Project: characterization of release of FGF1 or FGF2 by endothelial cell lines HUVEC and HAEC in response
to heat shock after adenoviral transduction by FGF1, FGF2 or Notch(ic domain)/FGF1 viruses.
 Minor project: characterization of the release of FGF1 by smooth muscle cell line HVSMC after adenoviral
transduction.
 Major techniques utilized: adenoviral transduction, heat shock of cell lines, protein extraction by heparinsepharose chromatography, LDH (lactate dehydrogenase) cytotoxicity assay, western blotting.
 Minor techniques utilized: RT-PCR, agarose gel electrophoresis, fluorescence staining, fluorescence and
confocal microscopy, luciferase assay, MaxiPrep isolation of plasmid.
U.H. Hilo College of Pharmacy (Hilo, HI)
11/08 – 06/09
Laboratory Technician
Principal Investigators: John M. Pezzuto, Ph.D. and Leng-Chee Chang, Ph.D.
 Conducted Cytotoxicity and Nitric Oxide (iNOS) assays to screen biological and synthetic compounds for
pharmaceutical activities.
 Analyzed and interpreted experimental data to determine significance of findings.
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Developed and presented reports of findings to Principal Investigators, and followed up with additional
analyses.
Extracted proteins and RNA from dosed tissue samples, determined in-vivo efficacy of novel therapeutic
compounds.
Created two manuals for lab’s new Eppendorf epMotion 5075 Automated Liquid Handler and trained lab
personnel on its use.
Taught student researchers the protocols for screening assays, cell culture techniques and data analysis.
Insured laboratory functions, including maintenance of stocks, reagents, labware, biohazards, and equipment
maintenance.
Management Recruiters of Woodland Hills, Inc. (El Dorado Hills, CA)
08/06 – 09/07
Owner, Partner, Account Executive Recruiter
 Negotiated and established working relationships with business clients to help with their recruiting needs.
 Utilized internet, telephone and email networks to recruit specialized employees for contracted client
companies.
 Established relationships with and marketed highly valuable candidates to desired potential clients.
 Worked with business partner and support companies to maintain the operation of my business franchise.
U.C. San Francisco Gyn. Oncology Dept. (San Francisco, CA) (05/01 – 09/02) & (10/03 – 08/06)
Staff Research Associate – Cervical Cancer Tissue Bank (Apr. 2006 – Aug. 2006)
Principal Investigator: Karen Smith-McCune, M.D. & Ph.D.
 Coordinated with surgeons, anesthesiologists, and pathologists at 2 hospitals to collect and preserve tissue
samples.
 Scheduled and met with patients to inform about research studies and obtained their consent for participation.
 Established and conducted various tissue preservation methods respective to desired research techniques.
 Developed and instituted catalog, database and archival systems for tissues, research data, and patient medical
information.
Staff Research Associate – Ovarian Cancer Tissue Bank (May 2001 – Aug. 2002) & (Oct. 2003 – Aug. 2006)
 Collaborated with surgeons, pathologists, anesthesiologists and nurses at 2 hospitals to collect and preserve
tissue samples.
 Helped improve methods to identify, consent, harvest from and follow-up with research participants.
 Collected and preserved blood, ascites, tumor tissues, epithelial cells, and normal tissues with fixation and
cryopreservation techniques.
 Maintained catalog, archive and database systems for tissues, research data and patient medical information.
 Cultured and characterized novel primary and commercially available cell lines with light and fluorescence
microscopy.
 Conducted regular mycoplasma testing on cell cultures and developed novel treatment methods for persistent
strains.
 Made paraffin cassettes of fixed tissues and cryogenic OCT molds of fresh tissues for microtome/cryostat
sectioning.
 Preserved DNA, RNA, and proteins from tissue samples and cell cultures via TriZol for analysis, archiving
and distribution.
 Utilized microtome, cryostat, and cell cultures for preparation and analysis of fresh, frozen and fixed tissue
slides.
 Used immunological and histological stains to examine tissue slides for microdissection, quality control, and
archiving.
 Insured general laboratory needs, including purchasing stock, reagents, and labware, as well as equipment
maintenance.
U.C. San Francisco Surgery Dept. (San Francisco, CA)
Staff Research Associate – Melanoma Vaccine Group
Principal Investigator: Stanley P.L. Leong, M.D.
08/00 – 05/01
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Per protocol, helped make 3 different melanoma vaccines using participating patients’ fresh blood and tumors.
Cultured primary cells from patient tumors, blood and lymphatic tissues with adjuvant factors.
Gamma-irradiated tumor cells and lymphocytes from tumor tissues and cell culture for vaccine development.
Performed ELISA assays for cytokine titre in patient plasma, for antigens MART1, GP100, CD8 and CD83 to
analyze vaccine efficacy and patient response.
Researched multiple tissue preservation techniques for improved RNA extraction from pathology samples.
Participated in weekly research presentations, reevaluation of research focus and scheduling of patient
vaccinations.
U.C. Santa Barbara Materials Engineering Dept. (Santa Barbara, CA)
04/98 – 07/00
Undergraduate Research Assistant
Principal Investigator: Cyrus R. Safinya, Ph.D.
 As an Undergraduate Research Assistant, volunteered with biological functions of this materials engineering
lab.
 Conducted transfections on mammalian cell cultures with novel liposomes and GFP proteins.
 Utilized live cell imaging techniques for fluorescence microscope using GFP and Hoechst dyes.
 Used microinjection techniques for introduction of DNA into live cell cultures with real-time imaging.
 Assisted in the assembly of an X-Ray diffractometer for crystallography of DNA-liposome complexes.
 General lab maintenance – oversight of stock, reagents, labware, and equipment maintenance.
U.C. Santa Barbara Biology Dept. (Santa Barbara, CA)
(Spring 1999) & (Spring 2000)
Teaching Assistant
Professor: Dennis O. Clegg, Ph. D.
 Teaching assistant for Cellular Biology Lab, a core upper-division biology course with appx. 24 students per
quarter.
 Guided by professor and a graduate student, helped teach prokaryotic and eukaryotic cell biology techniques:
hemacytometry, cell culture, protein spectrophotometry, western blotting, gel electrophoresis, chick embryo
dissection and neuron culture.
 Developed, assigned and graded homework and quizzes. Conducted and graded midterm and final exams and
helped assigned final grades.
 Held office hours to assist students with questions, difficulties and enrichment.
U.C. Santa Barbara Housing & Residential Services (Santa Barbara, CA)
09/99 – 06/00
Resident Assistant
 Oversight of one floor of an on-campus residence hall, assisting 50 residents in individual rooms and
communal suites.
 Provided leadership and educational opportunities to residents through active and passive programming and
mentorship.
 Assisted residents with creation of organizations including academic support groups and scholastic clubs.
 Mediated conflict resolution among room and hall-mates, and provided counseling to students
U.C. Santa Barbara Police Dept. (Santa Barbara, CA)
09/96 – 09/99
Community Service Officer (C.S.O.) and Area Coordinator
 Provided general campus safety via liaising with students and campus police officers and providing patrols of
university.
 Conducted daily and nightly rounds of on and off-campus housing, and lecture halls and campus departments
as assigned.
 Worked with police, sheriff and other law enforcement agencies to provide security for emergencies and
special events.
 Helped expand C.S.O. program as an Area Coordinator, advising on points of efficiency and assisting in
hiring processes.
 Purchased and serviced C.S.O. program bicycles as bicycle mechanic (April 1998 – September 1999).
Cinemark Movies 8 (Palmdale, CA) (Palmdale, CA)
(06/93 – 09/95) & (06/96 – 08/96)
Projectionist, Concessionist, Ticketeer, Usher
 Booth Projectionist, projecting films, assembling and disassembling film, maintaining marquee and
projectors.
 Ticketeer, selling tickets, dealing with refunds and conducting business promotions.
 Concessions, selling food and drink items, conducting stock surveys, and maintaining food service equipment.
 Usher, guiding customers, enforcing auditorium rules, cleaning auditoriums, conducting building
maintenance.
Research Articles, Posters and Presentations
Article title:
Inhibition of Nitric Oxide (NO) Production in Lipopolysaccharide (LPS)-Activated Murine Macrophage
RAW 264.7 Cells by the Norsesterterpene Peroxide, Epimuqubilin A
Authors:
Sarot Cheenpracha, Eun-Jung Park, Bahman Rostama, John M. Pezzuto and Leng Chee Chang
Publication (Open Access: www.mdpi.com/journal/marinedrugs)
Marine Drugs (ISSN: 1660-3397); 2010 Mar: Vol (8): 429-437
Article title:
Cancer chemopreventive and anticancer evaluation of extracts and fractions from marine macro- and
microorganisms collected from Twilight Zone waters around Guam
Authors:
Schupp PJ, Kohlert-Schupp C, Whitefield S, Engemann A, Rohde S, Hemscheidt T, Pezzuto JM, Kondratyuk TP,
Park EJ, Marler L, Rostama B, Wright AD
Publication (PMID: 20120114):
Natural Products Communications (ISSN 1934-578X); 2009 Dec: 4(12):1717-1728
Article title:
VDR Heterozygote Female Mice: an Experimental Model of Vitamin D Insufficiency; Investigation on Bone
Mass, Body Composition and Cardiac Function in Basal Conditions and Four weeks after PTH treatment
Authors:
Francisco J. A. de Paula, Ingrid Dick-de-Paula, Sheila Bornstein, Bahman Rostama, Phuong Le, Clifford J.
Rosen
Publication: Submitted, currently under review
Poster title:
Evaluation of anti-inflammatory activity of Pipiturus Albidus and Moringa Oleifera extracts
Authors:
Chaz Barit, Sarot Cheenpracha, Bahman Rostama, Tammy Tran, Marisa Wall, John M. Pezzuto, and Leng Chee
Chang
Presented by primary author at:
American Society of Pharmacognosy 50th anniversary meeting, June 27, 2009, Honolulu, HI
Poster title:
Cancer chemopreventive potential of novel natural product derivatives
Authors:
Laura Marler, Beau Rostama, Mark Cushman, Richard van Breemen, Clinton Grubbs, John Pezzuto
Presented by primary author at:
American Society of Pharmacognosy 50th anniversary meeting, June 27, 2009, Honolulu, HI
Poster title:
Mechanism-based screening of resveratrol derivatives as potential cancer chemopreventive agents
Authors:
Tamara P. Kondratyuk, Eun-Jung Park, Laura Marler, Bahman Rostama, John M. Pezzuto
Presented by primary author at:
American Society of Pharmacognosy 50th anniversary meeting, June 27, 2009, Honolulu, HI
Poster title:
Complementary and Alternative Medicine (CAM) Use is Prevalent in a Cohort of Women Undergoing Surgery in
an Ovarian Cancer Tissue Core Study
Authors:
B Rostama, CB Powell, LM Chen, N Karnik-Lee, JB Campbell, PA Glenn, K Smith-McCune
Presented by primary author at:
The Western Association of Gynecologic Oncologists (WAGO) annual meeting, June 2005, Santa Fe, NM
Education
Sept. 1995 – June 2000
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Conferred June 2000 - B.S. Biological Sciences, B.A. Global and International Studies
Sept. 2002 – June 2003
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University of California, Davis
Post-Baccalaureate courses – no degree sought
Aug. 2009 – present
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University of California, Santa Barbara
University of Maine, Orono
Graduate School of Biomedical Sciences (GSBS) Ph.D. graduate student; prospective graduation: 2015
Additional Training
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Primary Ovarian Surface Epithelium Cell Culture Techniques – Beth Karlan / RaeLynn Baldwin Lab, UCLA
Cedars Sinai Hospital, April 2004
Protein Microarray preparation, slide printing, and Immunohistochemistry – Gordon Mills Lab – M.D.
Anderson Cancer Center – January 2004
Health Insurance Portability and Accountability Act (HIPAA) training – UCSF, annual training, latest: 2006
Blood Borne Pathogens training – UCSF, annual training, latest: 2006
Human Subject Research training – NIH (online) and UCSF, annual training, latest: 2006
General Lab Safety training – University of Hawaii, annual training, latest: May 2009
Laboratory Radiation Safety Training – University of Hawaii, annual training, latest: May 2009
Human Subject Online Tutorial – University of Maine, latest: December 2009
Responsible Conduct in Research (RCR) – University of Maine/MMCRI: 2009 and 2010
CITI training: Health Information Privacy and Security for Clinical Investigators; Health Information Privacy
and Security for Students and Instructors; Working with Mice in Research Settings; Post-Procedure Care of
Mice and Rats in Research; Working with the IACUC for Investigators, Staff and Students: May 2010
Virus Core training – MMCRI: August 2010