國立彰化師範大學 95 學年度碩士班招生考試試題
系所： 生物技術研究所
科目： 分子生物學
☆☆請在答案紙上作答☆☆
共 4 頁，第 1 頁
一、單選題（1-10 每題 3 分，11-14 每題 2 分，共 38 分）
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國立彰化師範大學 95 學年度碩士班招生考試試題
系所： 生物技術研究所
科目： 分子生物學
☆☆請在答案紙上作答☆☆
共 4 頁，第 2 頁
11.
: Which statement was not truth about prokaryote translation pathway?
(a) The prokaryotic initiation factors 2 (IF-2) can bound GTP and ensure only initiator tRNA enter
partial P site of ribosome.
(b) The initiator tRNA was N-formylmethionine and it can bind to initiation factor IF-2 to form 30S
initiation complex.
(c) The ribosome can bind two tRNAs and about 35 bp mRNA.
(d) The prokaryotic elongation factors 2 (EF-G) can bound GTP and form ternary complex with
aminoacyl-tRNA.
(e) The prokaryotic releasing factors 3 (RF-3) can bound GTP and promote action of RF-1 and RF-2.
12.
: Which statement was not correct about prokaryotic transcription pathway?
(a) Transcription is catalyzed by RNA polymerase and it is consists of multiple subunits.
(b) RNA polymerase binds to one face of DNA.
(c) Sigma factor controls binding to mRNA.
(d) Substitution of sigma factors may control initiation.
(e) Bacteria RNA polymerase has two modes of termination.
13.
: Which is not correct about RNA polymerases in eukaryotes?
(a) Eukaryotes have 3 kinds of RNA polymerase but can’t recognize promoter region.
(b) RNA polymerase I was localized in nucleolus and transcribed 28S, 18S, 5S, and 5.8S rRNA.
(c) RNA polymerase II was localized in nucleoplasm and transcribed mRNA and snRNAs.
(d) RNA polymerase III was localized in nucleoplasm and transcribed tRNA, snRNA.
(e) RNA polymerase copies anti-sense (-) strand of DNA template and no primer required.
14.
: Which method can not be used for analysis of DNA-protein interaction?
(a) DNase I footprinting.
(b) Electrophoretic mobility shift assay.
(c) Yeast one-hybrid system.
(d) Primer extension.
(e) trans-Activation of reporter gene expression by ectopic overexpression of a transcription factor.
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國立彰化師範大學 95 學年度碩士班招生考試試題
系所： 生物技術研究所
科目： 分子生物學
☆☆請在答案紙上作答☆☆
共 4 頁，第 3 頁
二、問答題（共 27 分）
1. Why normal cells become tumor cells? Please provide at least 3 possible mechanisms. According to
your answer, please provide relative methods to inhibit oncogenic pathway. (6 %)
2. This figure was come from Minc et. al., J. Biol. Chem. 1999, 274(1):503-9.
According to the figure, please answer following questions:
What is the “normalized luciferase activity” that is shown on Y-axis. ? (2%)
If you have cloned the sod1 cDNA, how do you get its promoter region? (2%)
How to know the transcription start site of sod1 gene? (2%)
Please describe the results of this figure. (4%)
If you propose the transcription factor AP-1 can bind sod1 promoter between -71 to -29 bp, and
response to PMA-induced expression, please design a method to confirm your propose. (5%)
3. Describe briefly about sod 1 gene “knock-in”, “knock-down”, and “knock-out” in mouse. (6%)
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國立彰化師範大學 95 學年度碩士班招生考試試題
系所： 生物技術研究所
科目： 分子生物學
☆☆請在答案紙上作答☆☆
共 4 頁，第 4 頁
三、Molecular Biology(共 35 分)
1. Which steps require hydrolysis of GTP in protein synthesis?
(4%)
2. Please describe the SOS response system. (10%)
3. Please describe the Post Translational Modification. (8%)
4. Please describe the stages in the lytic life cycle of a typical phage. (8%)
5. Please describe the Transposon.
(5%)
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