University of Virginia Biosafety Manual
And Standard Operating Procedures
For Biosafety Level 2 Agents
Dr. J. Thomas Parsons
University of Virginia Microbiology Department
Rooms 223, 224, 2216B Jordan Hall
Charlottesville, VA
March 26, 2008
2
University of Virginia
Biosafety Manual
Principal Investigator
Lab Building/Rooms
Dr. J. Thomas Parsons
Jordan Hall/ Rooms 223, 224, 2216B
Agents used:
BSL-2 Microorganisms
Rooms 2216B & 223
Adenovirus
Lentivirus
Human-derived materials
Room 224
A549
IGROV
Asp-Pc1
Ishikawa
BxPc3
L3.6pl
CaOV-3
LNCap
Colo357
LP-9
DU145
MCF-7
ES-2
MD-MBA-231
FG
MD-MBA-468
H23
MeT5A
H460
MPanc 96
H1650
OvCar-3
H1975
Panc1
HEC-1A
PC3
HEC-50co
PC12
HEK293
RL-95
HeLa
SKOV-3
SK-UT
Biotoxins/room
None
Principal Investigator’s Certification
I hereby certify that I have reviewed the contents of this manual and that it reflects
my current operating practices.
Signature____________________
Date_________________
Annual Review:
Signature____________________
Date_________________
Signature____________________
Date_________________
3
Contact Information
Office of Environmental Health and Safety 982-4911
Institutional Biosafety Committee (IBC) 982-1597
UVA-WorkMed (formerly Occupational Health Services) 243-0075
UVA Employee Health 924-2013
Student Health Center 924-5362
Principal Investigator’s Home Phone 540-248-1225
4
SIGNATURE and ACKNOWLEDGEMENT PAGE
Authorization
All members of the J.T. Parsons Lab who have signed the list below are approved for
entry into Rooms 2-23, 2-24, and 2-2216B while work with BSL 2 agents is in progress.
HOWEVER, only those persons who have attended the OSHA Bloodborne Pathogens
lecture and are otherwise specifically trained (e.g., the University Biosafety Officer
provides a short training program entitled, “Fundamentals of Biosafety”) may perform
work with samples or cell cultures in Rooms 2-23, 2-24, 2-2216B.
Anyone (including any workers not in the P.I.’s Lab) who uses Rooms 2-23, 2-24, and
2-216B must sign the disclaimer below.
Disclaimer
We, the undersigned, understand that adenovirus and lentivirus are in use in the Tissue
Culture Room (2-23), Room 2-24, and Room 2216B on the second floor of Jordan Hall,
and that these agents are infectious to humans. We agree to follow BL2 containment
when using adenoviruses, lentiviruses, or virus-infected cells, and to follow appropriate
precautions to prevent inadvertent exposures to these agents.
Further, we have read and understood this manual and agree to attend required
Bloodborne Pathogens lectures prior to handling samples in Rooms 2-23, 2-24, and
2216B, Jordan Hall.
Name
Todd Bauer
Cheryl Borgman
Catherine Cowan
Pablo Grigera
Christine Harrer
Dan Hershey
Linda Patchel
Amy Koski
Marcin Iwanicki
Tom Parsons
Jill Slack-Davis
Jayme Stokes
Rob Tilghman
Signature
Date
HBV
Yes/No/ Declined?
5
6
Authorization and Disclaimer for Weber Laboratory
We, the undersigned, understand that adenovirus and lentivirus (low titers) are in use in
the Tissue Culture Room (2-23), and Room 2-24, on the second floor of Jordan Hall. We
have been informed that the Biosafety Manual for the JT Parsons laboratory is available
for review and is located in room 225 A and on the JT Parsons lab web page
(http://faculty.virginia.edu/tparsons/). We agree to follow BL2 containment when using
adenoviruses, lentiviruses, or virus-infected cells and to follow appropriate precautions.
Name
Signature
Date
7
8
Rooms 223 and 232
9
Rooms 224 & 225
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Table of Contents
Principal Investigator Certification and Agent Inventory
2
Signatures and Acknowledgement of Risk
4, 5
Biosafety Manual (must be completed by all Investigators working with BSL2
microorganisms and/or human-derived materials and/or toxins of biological)
origin)
1. Purpose
2. Responsibilities
3. Background
4. Work Practices
A. Standard Practices for BSL-2 agents
B. Safety Equipment
C. PPE
D. Lab Standard Operating Procedures
5. Waste Disposal
A. Liquids
B. Sharps
C. Solid Wastes
D. Research Animal Carcasses
6. Spill Clean Up Procedures
A. Spills Outside of a Containment Device
B. Spills inside a Biosafety Cabinet
C. Spills in a Centrifuge
D .Biological/Radioactive Emergencies/Spills
7. Proper Use of a Biosafety Cabinet
8. Emergency Phone Numbers and Procedures
A. Fire
B. Injury
C. Exposure to Biohazardous Materials
D .Security Incidents
9. Shipping Infectious Substances
11
11
12
14
14
16
16
17
17
18
18
18
19
19
19
20
20
21
21
22
23
23
23
23
24
Appendix A. Exposure Control Plan (must be completed by investigators
working with human-derived materials including human cell lines)
25
Appendix B. Toxin Safety Plan (must be completed by investigators working
with toxins of biological origin)
37
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1. Purpose
This Biosafety Manual outlines procedures for using and disposing of Biosafety
Level 2 (BSL-2) agents. All University laboratories using BSL-2 agents must
comply with the procedures in this manual. Principal Investigators or laboratory
supervisors must contact the Office of Environmental Health & Safety if they are
uncertain how to categorize, handle, store, treat or discard any biohazardous
material.
2. Responsibilities
The Principal Investigator:
1. Ensures that laboratory and support personnel receive appropriate
training for the potential hazards associated with the work involved, the
necessary precautions to prevent exposures, and the exposure
evaluation procedures.
2. Ensures biosafety procedures are incorporated into standard operating
procedures for the laboratory.
3. Ensures personal protective equipment and necessary safety equipment
is provided and used.
4. Ensures compliance by laboratory personnel with the relevant
regulations, guidelines, and policies
5. Reviews and updates the Biosafety Manual annually.
6. Submits a written report to the Institutional Biosafety Committee
concerning:
 Any accident that results in inoculation, ingestion, and inhalation of
biohazardous materials or recombinant DNA
 Any incident causing exposure of personnel or danger of
environmental contamination.
 Any problems pertaining to operation and implementation of biological
and physical containment safety procedures or equipment or facility
failure.
Laboratory Personnel
1. Participates in appropriate training and instruction
2. Complies with biosafety procedures.
3. Reports all accidents, major spills, or exposure incidents to supervisor
4. Reviews the Biosafety Manual annually.
Additional Responsibilities for this lab -
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3. Background
The focus of the laboratory of Dr. J. Thomas Parsons is to study adhesion
signaling as it relates to cell growth, migration and metastasis. The potentially
infectious biological materials used in this work include viral vectors (adenovirus
and retrovirus), and human cells and tissues which will be handled at the BSL2
level. Also, BSL 1 level agents are used such as recombinant DNA, E.coli K12,
and baculovirus. Specific details regarding the use of BSL2 level agents is
described below.
Avian retroviruses are commonly used to transfer new genetic constructs
into cultured chick embryo fibroblast cells (CEF). The constructs used in the
Parsons laboratory use a vector system, RCAS-A and RCAS-B, designed by
Hughes, et al (J. Virol. 61:3004-3012) in which the gene of interest is cloned into
a replication-competent plasmid and transfected into CEF cells. This results in
the expression of the introduced gene in CEF cells and also the production of
recombinant infectious virus, containing the viral recombinant RNA genome and
the viral structural proteins. Virus generated by this procedure is used in further
infections of CEF cells. These avian retroviruses are restricted to avian cell lines
and are NOT capable of infecting cells of other species. Nevertheless, it is
prudent that these viruses and infected cells be handled as a potential hazard if
the gene introduced is biologically active (e.g. an oncogene). Examples of such
oncogenes used in the laboratory include the tyrosine kinase Src and the small
GTPases Ras and Cdc42. Thus, RCAS virus stocks, infected and transfected
CEF cells will be handled at the BL2 level of containment, as described below.
Human Adenovirus Type 5 (Ad5) will be employed to transfer genetic
constructs into mammalian cancer cell lines and primary rodent cells. The Ad5
genome (100 map units, mu) containing a 4.3 kB insert which renders the virus
defective in packaging will be co-transfected into human HEK293 cells with a
shuttle plasmid containing the CMV promoter and the coding sequence of the
gene of interest flanked by 0-1 mu and 9-16 mu of the Ad5 genome. Following
recombination, the cassette will replace the 4.3 kB insert and the E1 region of the
genome, making the virus capable of packaging but replication defective. The
HEK293 cell line is Ad5 transformed and expresses the E1 region (1-11.3 mu) in
trans which allows for replication of the recombinant adenovirus. Virus is
collected from cells and purified by cesium-chloride gradient. Virus is then
incubated with the cells of interest at 20-100 plaque forming units/cell. The Ad5
virus stocks, infected HEK293 packaging cells and target cells will be handled at
the BSL2 level of containment, as described below.
Retroviruses will be used to infect rodent and human cells lines to allow for over
expression of defined proteins or expression of small interfering RNAs to
selectively suppress the expression of targeted proteins. Appropriate virus
stocks are generated using transfection of HEK293 cells with three separate
plasmids, each containing a different component of the virus. The transducing
vector is pLKO.1 puro (Sigma); it contains a hairpin insert corresponding to the
gene to be targeted, an RRE rev response element, self-3' inactivation long
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terminal repeat and RNA packaging signal. The packaging plasmid is
CMVdeltaR8.2 (obtained from David Rekosh, UVa. It has HIV-1 structural
proteins, NEF and Rev downstram of CMV promotor. The envelope vector, MDg
(David Rekosh) encodes VSV-G under beta globin promotor. These plasmids
are transfected into HEK293 T cells and supernatants are collected 48 hrs later.
Transfection of HEK293 cells with the three plasmids generates replication
defective virus particles that can be used to infect human or rodent cells to effect
the knockdown of selected genes in the target cells. The tripartite vector system
ensures that there is no infectious virus produced. Generation of the virus and
subsequent infection of target cells is carried out using BSL2 containment.
Human cell lines and archival human tumor samples will also be handled
at the BSL 2 level because of the possibility that they contain unidentified
etiologic agents.
The Parsons laboratory does not use any biotoxins regulated by the
Antiterrorism and Effective Death Penalty Act of 1996.
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4. Work Practices
Many laboratory procedures using BSL-2 agents can be safely done on the open
laboratory bench utilizing good microbiological techniques and appropriate
Personal Protective Equipment (PPE) provided the potential for producing
splashes or aerosols is low. Work on the open laboratory bench is permitted,
except when performing activities with BSL-2 agents that may produce aerosols
or splashes.
Procedures with a potential for creating infectious aerosols or splashes must be
conducted in a certified Biosafety Cabinet (BSC). These may include
centrifuging, grinding, blending, vigorous shaking or mixing, sonic disruption,
opening containers of infectious materials whose internal pressures may be
different from ambient pressures, inoculating animals intranasally, and harvesting
infected tissues from animals or embryonate eggs.
A. Standard Microbiological Practices for Biosafety Level 2 agents
1. Access to the laboratory is restricted when work with BSL-2 agents is in
progress. Access may be restricted by locking doors, posting warning
signs or using other suitable methods as determined by the Principal
Investigator.
2. A biohazard sign must be posted on the entrance to the laboratory when
BSL-2 agents are in use. Information to be posted includes the agent(s)
in use, the Biosafety level, the investigator's name and telephone
number, and any personal protective equipment that must be worn in the
laboratory.
3. Persons wash their hands after they handle viable materials, after
removing gloves, and before leaving the laboratory.
4. Eating, drinking, smoking, handling contact lenses, and applying
cosmetics are not permitted in the laboratory. Food is stored outside the
laboratory.
5. Mouth pipeting is prohibited; mechanical pipeting devices are used.
6. All procedures are performed carefully to minimize the creation of
splashes or aerosols.
7. Work surfaces are decontaminated with disinfectants that are effective
against the infectious agents present upon completion of work or at the
end of the day and after any spill or splash of viable material
8. Chairs and other furniture are covered with a non-fabric material that can
be easily decontaminated. Carpets and rugs in laboratories are
inappropriate.
9. All cultures, stocks, contaminated wastes and other Regulated Medical
Waste (RMW) are disposed of in accordance with University of Virginia
Infectious Waste Disposal Procedures (see page 15).
15
10. A sharps management program is in place including:
a. Needles and syringes or other sharp instruments should be
restricted in the laboratory for use only when there is no alternative.
b. Used disposable needles must not be bent, sheared, broken,
recapped, removed from disposable syringes, or otherwise
manipulated by hand before disposal; rather, they must be carefully
placed in a conveniently located Sharps Container. Non-disposable
sharps must be placed in a hard-walled container for transport to a
processing area for decontamination, preferably by autoclaving.
c. Syringes which re-sheathe the needle, needleless systems, and
other safety devices are used when appropriate.
d. Broken glassware must not be handled directly by hand, but must
be removed by mechanical means such as a brush and dustpan,
tongs, or forceps.
11. Cultures, tissues, specimens of body fluids, or potentially infectious
wastes are placed in a container with a cover that prevents leakage
during handling, processing and storage.
12. Transport of BSL-2 agents to sites within the grounds of UVA, must be
placed in a secondary leak proof carrier that can contain the contents if
the primary container were to leak or break. Carriers must have the
biohazard label affixed to the outer surface of the transport container
13. Contaminated equipment must be decontaminated before it is sent for
repair or maintenance or before removal from the laboratory.
14. Spills and accidents that result in overt exposures to infectious materials
are immediately reported to the Principal Investigator and UVAWorkMed.
15. Animals not involved in the work being performed are not permitted in
the lab.
16. Additional Special Practices for this Laboratory
 Spills and accidents which result in overt exposure to virus containing
oncogenic recombinant DNA molecules will be immediately reported
to J.T. Parsons.
 Medical evaluation, surveillance and treatment will be provided as
outlined in the CDC-NIH Biosafety Manual for personnel at risk.
 Infectious agents will not be mixed with radiochemicals.
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B. Safety Equipment
1. Properly maintained and annually certified biological safety cabinets,
preferably Class II, or other appropriate personal protective equipment or
physical containment devices are used whenever procedures with a
potential for creating infectious aerosols or splashes are conducted.
These may include centrifuging, grinding, blending, vigorous shaking or
mixing, sonic disruption, opening containers of infectious materials
whose internal pressures may be different from ambient pressures,
inoculating animals intranasally and harvesting infected tissues from
animals or embryonate eggs.
2. Centrifugation presents a physical hazard in the event of mechanical
disruption. Aerosols and droplets may also be generated. High
concentrations or large volumes of infectious agents may be centrifuged
in the open laboratory if sealed rotor heads or centrifuge safety cups are
used, and if these rotors or safety cups are opened only in a biological
safety cabinet.
C. Personal Protective Equipment (PPE)
1. Laboratory coats or gowns are worn while work is performed in the
laboratory. Lab coats are removed before leaving for non-laboratory
areas (e.g., cafeteria, library, administrative offices). All protective
clothing is either disposed of in the laboratory or laundered by an
approved outside vendor. Lab coats are never taken home by
personnel. Prior to sending out for laundering, contaminated coats must
first be decontaminated by autoclaving or soaking in a freshly prepared
10% bleach solution for 10 minutes.
2. Gloves are worn when hands may contact potentially infectious
materials, contaminated surfaces or equipment. Gloves are disposed of
when overtly contaminated, and removed when work with infectious
materials is completed or when the integrity of the glove is
compromised. Disposable gloves are not washed, reused, or used for
touching "clean" surfaces (keyboards, telephones, etc.), and they
should not be worn outside the lab. Hands are washed following
removal of gloves.
3. Face protection (goggles, mask, face shield or other splatter guard) is
used for anticipated splashes or sprays of infectious or other hazardous
materials to the face when the BSL-2 agents must be manipulated
outside the BSC
4. Respirators are generally not required when working with BSL-2 agents.
Respirators should only be used after consultation with the Office of
Environmental Health and Safety. Fit testing, respirator training and
annual medical surveillance are required.
17
D. Laboratory Standard Operating Procedures
Describe the laboratory procedures using BSL2 agents that will be performed in
a BSC and the PPE used:
 Cell cultures will be
 Work with BSL 2 agents in open containers will only be done in the
designated hood in Room 223 Jordan.
 Gloves and laboratory coats will be worn when working with live virus
or virus-producing cells.
 No razor blades, scalpels, or hypodermic needles are permitted while
working with live virus.
Describe the laboratory procedures using BSL2 agents that will be performed on
the open bench and the PPE used:
Living BSL 2 agents will not be handled on open lab benches, but only in a
certified biosafety cabinet.
List the disinfectant used for routine decontamination :
 Work surfaces will be decontaminated after each use with 70%
ethanol. In addition, the surface of all equipment used in
manipulations will be decontaminated with 70% ethanol.
 Materials that come into direct contact with the virus will be
decontaminated with bleach (0.3% NaOCl) and allowed to stand for
15 minutes before autoclaving in a biohazard bag.
 Decontamination of vessels containing liquid will be achieved by
adding bleach to a final concentration of at least 0.3%. After 15
minutes, bleached liquid will be disposed into the drain.
5. Infectious Waste Disposal
Infectious Waste or “Regulated Medical Waste” (RMW) is defined as any
waste materials that are capable of producing a disease by an organism
likely to be pathogenic to humans, such as the following:
1. Discarded cultures and stocks of microorganisms, specimens,
vaccines and associated items containing organisms likely to be
pathogenic to healthy humans.
2. Human blood and certain body fluids as defined by OSHA.
3. Items saturated or caked with human blood or body fluids that would
release blood/body fluid in a liquid or semi liquid state if compressed
or would flake if handled.
4. Human tissue, human cell lines or anatomical wastes
5. Sharps (needles, syringes with attached needles, and scalpel
blades, etc).
18
6. Animal carcasses, body parts, bedding and related wastes when
intentionally infected with organisms pathogenic to healthy humans.
7. Any residue that results from the clean up of a spill of infectious
waste.
8. Any waste contaminated by or mixed with infectious waste.
The disposal of RMW is both highly regulated and very costly. University
of Virginia faculty, staff and students must use the utmost care to
segregate all waste materials properly
A. Liquids: Durable, leak proof containers will be used to receive liquid
waste. Liquids are decontaminated by adding bleach to a final concentration
of 10% or other appropriate disinfectant to a final concentration
recommended by the manufacturer. Mix well and allow to stand for fifteen
minutes or recommended time, whichever is greater. Pour decontaminated
liquid into the sink and rinse with copious amounts of cold water. Liquid
waste that is not compatible with disinfectant must be autoclaved for at least
30 minutes, using slow exhaust before disposal.
B. Sharps: (needles, syringes with attached needles, and scalpel blades,
scissors lancets, guidewires, contaminated glass pasture pipettes, etc).
Disposable sharps must be placed in a plastic sharps container as soon
after use as possible. Approved sharps containers are available from the
UVA Hospital Storeroom. Sharps containers must be located as close as
possible to the area where sharps are used. When the sharps container is a
¾ full, close it and place it a UVA “Contaminated Materials Container”
(CMC) for final disposal.
C. Solid Wastes: Solid infectious waste material (other than sharps) may
be autoclaved in the lab in clear autoclave bags and then disposed of as
regular trash. The outside of the receptacles containing the autoclave bag
should be marked with the biohazard symbol until processing has been
completed. Alternatively, solid infectious waste may be disposed of directly
into a UVA “Contaminated Materials Container” (CMC) CMCs are available
from the UVA Hospital Storeroom. These containers must be lined with red
bags. Once a CMC is full, the top must be taped closed and properly
labeled with the generator’s name, building, room number, phone extension
and date. CMCs are either removed and managed by UVA Environmental
Services Department or brought to the appropriate storage area for your
building (contact the Office of Environmental Health & Safety, OEHS 2-4911
for information on this location) where it will be removed and managed by
OEHS. If the outside of the CMC becomes contaminated, the waste must
be repackaged by placing it into a secondary container that meets all the
requirements of CMCs. CMCs are sterilized at the Hospital Waste Sterilizer.
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D. Animal Carcasses: Research Animal carcasses that have been
intentionally infected with organisms likely to be pathogenic to healthy
humans, inoculated with human-derived materials, or are otherwise
considered ABSL-2 must be put in red plastic bags and disposed of in the
vivarium cold room or designated freezer. Contact the Center for
Comparative Medicine for additional information and guidance.
6. Biohazard Spill Clean up Procedures
All major spills must be reported to the Principal Investigator
A major spill is one in which: the spill splashes over an area larger than one
foot in diameter, the extent of the spill is undetermined, or the spill involves an
aerosol. All spills must be described in your Biosafety Manual. The
description must include: 1) the type of spill, 2) the time and date it happened,
3) the time and date it was cleaned up, and 4) the time and date you
autoclaved the waste from the spill.
A. Spills outside of a containment device:
1. Close off spill area to traffic, and notify coworkers.
2. If the spill may involve an aerosol, leave the room for 30 minutes to
allow aerosols to settle.
3. Remove contaminated clothing and wash exposed skin.
4. Put on gloves and lab coat.
5. Contain the spill with paper towels.
6. Flood the spill area with a freshly prepared 1:10 dilution of chlorine
bleach. If dilution is not possible, undiluted household bleach can be
used taking care not to splash the bleach onto oneself. Leave on for
10 minutes.
7. Push the towels at the edge of the spill into the spill's center. Add
more paper towels as needed.
8. Discard the paper towels into a regulated medical waste container
(CMC). If glass is present, do not use bare hands! Use forceps or a
dustpan to remove large pieces. Leave small pieces for clean up by
Environmental Services, cover with paper towels and block off area.
9. If contact with bleach occurs with skin, mucous membranes or eyes,
flush area with copious amounts of water.
10. Discard gloves into regulated medical waste container. Wash hands
thoroughly.
11. Report incident to supervisor and Principal Investigator.
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B. Spills inside of a Biological Safety Cabinet
Prepare a fresh dilution of 1:10 bleach, or use another appropriate nonrinse disinfectant. Allow sufficient contact time (10 minutes for bleach). If
using another disinfectant, manufactures directions must be followed.
1. Put on gloves and lab coat.
2. Disinfect the contaminated absorbent paper toweling if present then
discard it into the biohazard bag or CMC.
3. Wipe down the cabinet interior and any items inside the BSC with a
towel dampened with disinfectant
4. The spilled fluid and disinfectant solution on the work surface must
be absorbed with paper towels and discarded into a CMC.
5. Spills large enough to result in liquids flowing through the front or rear
grilles require more extensive decontamination; consult OEHS for
guidance.
C. Spills in a centrifuge
1. Wait at least 30 minutes after centrifuge has stopped before opening
(or reopening if breakage is discovered after the machine has
stopped).
2. Before attempting to deal with the leak, prepare a pan of non-rinse
sanitizer disinfectant solution large enough to immerse the entire
bucket for at least 20 minutes (bleach can cause corrosion).
3. Don any protective gear needed to clean up spills. If the rotor is
sealed, and removable, place it in the biosafety cabinet before
opening and carefully remove the lid from the rotor. If the rotor is not
removable, remove the buckets and place them in the BSC.
4. Retrieve unbroken tubes, wipe outside with disinfectant, and leave
them in the cabinet, out of the way. The broken tube must be
removed with a forceps or other instrument if possible and immersed
in a beaker of disinfectant solution for a minimum of fifteen minutes.
Do not use bare hands.
5. All instruments and rotor pieces involved in the incident must be
chemically decontaminated before re-use. After proper
decontamination, instruments and rotor pieces may be washed with a
mild detergent according to the manufacturer’s instructions.
6. The inside of the centrifuge chamber must be wiped out with
disinfectant.
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D. Biological/Radioactive emergencies/spills
The Radiation Safety Office (982-4911) must be notified and will assist in
the clean up of a biological/radioactive spill. Determine if anyone has been
contaminated; remove contaminated clothing and wash contaminated skin
with soap and water. Proceed with clean up as instructed by the Radiation
Safety Office. The infectious agent will be neutralized first, taking care in
choosing a disinfecting agent to avoid chemical incompatibility. Chlorine
compounds such as bleach must NOT be used to disinfect anything
containing 125I because the chlorine will cause the volatilization of
radioactive iodine.
7. Proper use of the Biological Safety Cabinet (BSC)
Biosafety Cabinets must be certified annually. BSCs are certified by an outside
contactor at no cost to the Investigator. Contact OEHS for details.
1. BSC fans must run for 10-15 minutes prior to working in the cabinet and
after completion of work.
2. Minimize other activities in the room (e.g., rapid movement, open/closing
room doors, etc.) to avoid disrupting the cabinet air barrier.
3. Laboratory coats should be worn buttoned over street clothing; gloves are
worn to provide hand protection.
4. Before beginning work, the investigator must adjust the stool height so
that his/her face is above the front opening.
5. Plastic-backed absorbent toweling can be placed on the work surface (but
not on the front or rear grille openings). This toweling facilitates routine
cleanup and reduces splatter and aerosol formation during an overt spill.
6. Closure of the drain valve under the work surface must be done prior to
beginning work so that all contaminated materials are contained within the
cabinet should a large spill occur.
7. Place necessary materials in the BSC before beginning work. This serves
to minimize the number of arm-movement disruptions across the air
barrier of the cabinet. All materials must be placed as far back in the
cabinet as practical, toward the rear edge of the work surface and away
from the front grille of the cabinet
8. The front grille must not be blocked with research notes, discarded plastic
wrappers, pipeting devices, etc.
9. Aspirator suction flasks must contain an appropriate disinfectant, and an
in-line filter. This combination will provide protection to the central building
vacuum system or vacuum pump, as well as to the personnel who service
this equipment. Inactivation of aspirated materials can be accomplished by
placing sufficient chemical decontamination solution such as bleach, into
the flask to kill the microorganisms as they are collected. Once inactivation
occurs, liquid materials can be disposed of as noninfectious waste.
22
10. Horizontal pipette discard trays containing an autoclave bag or an
appropriate chemical disinfectant should be used within the cabinet.
Upright pipette collection containers placed on the floor outside the
cabinet or autoclavable biohazard collection bags taped to the outside of
the cabinet should not be used. The frequent inward/outward movement
needed to place objects in these containers is disruptive to the integrity of
the cabinet air barrier and can compromise both personnel and product
protection.
11. All operations should be performed on the work surface at least four (4)
inches from the inside edge of the front grille.
12. Active work should flow from the clean to contaminated area across the
work surface. Bulky items such as biohazard bags, discard pipette trays
and suction collection flasks must be placed to one side of the interior of
the cabinet.
13. Open flames are rarely necessary in the near microbe-free environment of
a biological safety cabinet. An open flame creates turbulence that disrupts
the pattern of HEPA-filtered air supplied to the work surface. When
deemed absolutely necessary, touch-plate microburners equipped with a
pilot light to provide a flame on demand may be used. Internal cabinet air
disturbance and heat buildup will be minimized. The burner must be
turned off when work is completed.
14. Clean Up: Upon completion of work, the final surface decontamination of
the cabinet must include a wipe-down of the interior surfaces.
Investigators must remove their gloves and gowns in a manner to prevent
contamination of unprotected skin and aerosol generation and wash their
hands as the final step in safe microbiological practice. Investigators must
determine the appropriate method of decontaminating materials that will
be removed from the BSC at the conclusion of the work.
8. EMERGENCY PHONE NUMBERS AND PROCEDURES
Emergency Phone Numbers
Fire and Medical Emergencies within the Medical Center ….. 924-2012
Fire and Medical Emergencies.................................................9+911
Police……………………………………………………………… 9+911
Principal Investigator’s Home Phone…………………............. 540-248-1225
UVA-WorkMed (formerly Occupational Health Services) ……243-0075
UVA Employee Health……………………………………………924-2013
Hospital Emergency Room……………………………………… 924-2231
Office of Environmental Health and Safety……………………. 982-4911
Evacuation Meeting Place for Laboratory Personnel –
Jefferson Park Avenue entrance of Jordan Hall.
23
A. Fire
In case of fire, activate the fire alarm pull station and evacuate immediately.
Judgment should be exercised in deciding whether to store or contain any
hazardous materials prior to evacuation. Remove contaminated protective
garments and gloves before leaving laboratory if possible.
B. Injury
If an injury is life threatening call 9-911.
For less serious injuries treatment should be sought at UVA-WorkMed
(formerly Occupational Health Services) or UVA Employee Health during
weekday, daytime hours. During night and weekend hours, treatment should
be sought at the UVA Hospital Emergency Room. Any injury to a laboratory
worker shall be reported as soon as possible to the Principal Investigator.
C. Exposure to Biohazardous material
An exposure is defined as: BSL-2 agent contact with broken skin, eyes, nose,
mouth, other mucous membranes, a percutaneous injury with a contaminated
sharp, or contact with an infectious agent over a large area of apparently
intact skin.
In the event of exposure:
1. Wash the area with soap and water or flush eyes, nose or mouth with
large amounts of water for 15 minutes.
2. Staff must report immediately to UVA-WorkMed or UVA Employee
Health, or after hours to the Hospital Emergency Room. Students must
report to the Student Health Center 8am-5pm Monday-Friday or to the
Emergency Room after hours.
3. All exposures must be reported to the immediate supervisor and
Principal Investigator.
4. Principal Investigators are responsible for reporting exposure incidents
to the Institutional Biosafety Committee.
D. Security incidents
Security incidents such as suspicious visitors, missing chemicals, or missing
infectious agents must be promptly reported to the Principal Investigator.
University Security or Police should be notified. Principal Investigators are
responsible for reporting incidents to the Institutional Biosafety Committee..
24
9. Shipping Biological and Infectious Substances
The transportation of biohazardous substances is regulated by the U.S. Public
Health Service, the Department of Transportation and the International Air
Transport Association (IATA).
University personnel who ship infectious substances including diagnostic
specimens, microorganisms, human-derived materials, etc. must complete a
training program. The Office of Environmental Health and Safety offers a
regularly scheduled course and also has a proprietary training module available
on compact disc. Individuals who complete training must retain documentation
confirming that they passed the final exam. Training is valid for two years.
This Lab does not ship Infectious substances, Diagnostic Specimens or
Biological Agents (includes Human-derived materials and Human cell lines).
X This Lab ships Infectious substances, Diagnostic Specimens or Biological
Agents (includes Human-derived materials and human cell lines). List Personnel
trained, date of training and attach the training certificate.
Shipping Infectious Substances and Diagnostic Specimens Training
Training Certificate on following page.
Name
Linda Patchel
Date
April 19, 2007
25
Appendix A Exposure Control Plan (must be completed by
investigators working with human-derived materials including human cell lines)
Table of Contents
I.
II.
III.
IV.
V.
Vl.
VIl.
Vlll
lX.
X.
Introduction
Exposure Determination
Methods of Compliance
A. Universal Precautions
B. Engineering and Work Practice Controls
Communication of Hazards to Employees
Hepatitis B Vaccination Policy
Procedures for Exposure Incidents
Employee Training
Recordkeeping Procedures
Hepatitis B Vaccine
Safe Medical Devices Available
25
25
26
26
26
29
30
30
31
31
33
34
26
I.
Introduction
This Exposure Control Plan was prepared by the University of Virginia to
reduce employee exposure to bloodborne pathogens in accordance with
OSHA standard 29 CFR 1910.1030 and Virginia state law. This plan must
be reviewed by the Principal Investigator annually and in the event of
interim changes. A copy of this plan is available for review by any
employee during their work shift.
Principal Investigators have 15 days to provide a written copy of this plan
to any employee who requests it.
II.
Exposure Determination
This plan covers all employees who may reasonably be anticipated to be
at risk for exposure to human blood, or other potentially infectious
materials (“OPIM”, see list below). Employees determined to be at risk for
exposure must be offered the Hepatitis B vaccine at no charge to the
employee and must receive annual Bloodborne Pathogens training.
Principal Investigators must determine whether an employee has the
potential for exposure without considering the use of personal protective
equipment (PPE).
Other Potentially Infectious Materials (“OPIM”)
Human tissue or organs (unfixed)
Human tissue cultures
Human blood components
Blood, organs or tissue from research animals inoculated with human cell
lines, human tissue
Blood, organs or tissue from research animals infected with HIV, HBV,
HCV or other human pathogens
Semen and vaginal secretions
Cerebrospinal fluid
Pleural and pericardial fluid
Peritoneal fluid
All body fluids visibly contaminated with blood
Check the job classifications in the lab with potential for exposure to human
blood/OPIM:
X
Laboratory and Research Aide
X
Laboratory and Research Specialist
X
Laboratory and Research Manager
X
Post-Doctoral Fellow
27
X
X
X
Professor
Research Associate
Student (specify) Independent study students, work-study students
Other (specify)
Check the tasks and procedures performed by the employees listed above in
which occupational exposure occurs
X
Handling and manipulating human blood or OPIM
Other (specify)
III.
Methods Of Compliance
Principal Investigators and all staff will comply with the OSHA Bloodborne
Pathogens Standard 29 CFR 1910.1030 using the following methods:
A. Universal Precautions
All human blood and OPIM are considered contaminated with bloodborne and
other pathogens. Employees must avoid direct contact with human blood, and
OPIM to avoid exposure to bloodborne and other human pathogens.
B. Engineering and Work Practice Controls
Engineering controls and safe work practices will be used to minimize exposure
to human blood, and OPIM. Sharps and other medical devices that incorporate
built-in safety features and that have been evaluated and approved by the
University of Virginia Health Sciences Center Safety Committee are available
from the UVA Hospital Storeroom. Safe work practices are reviewed by the
Office of Environmental Health and Safety (OEHS) and the Institutional Biosafety
Committee (IBC).
1. Personal protective equipment.
If the potential for exposure remains in spite of work practice and engineering
controls, personal protective equipment (PPE) must be used. Employers must
provide, clean and dispose of PPE at no cost to the employee. PPE must be
worn during procedures in which human blood or OPIM exposure to skin, eyes,
nose or mouth is reasonably anticipated. PPE must be selected based on the
type of exposure anticipated. PPE must cover all body parts and street clothes
that may be exposed and must prevent soak through. Gloves, fluid-resistant
gowns, face shields, masks, and other types of PPE are available from the UVA
Hospital Storeroom. Non-latex gloves are available for employees with latex
sensitivity or allergy. PPE and personal clothing must be removed if they
become contaminated. Disposable PPE that is contaminated must be
discarded in a UVA Regulated Medical Waste Container (CMC). Reusable
PPE such as goggles and lab coats that have become contaminated must be
placed in a specified container for decontamination and reprocessing.
28
a. Lab coats and gowns. Lab coats or gowns must be worn when working with
human blood or OPIM when the potential for exposure exists. All PPE
should be removed before leaving the laboratory for non-laboratory areas
(office, library cafeteria etc.).Home laundering of lab coats and other PPE is
not permitted. Contaminated Laundry must be handled as little as possible
and gloves must be worn. Contaminated lab coats or other PPE sent off site
for laundering must first be decontaminated by soaking in a 1:10 diluted
fresh chlorine bleach solution for 10 minutes or by steam sterilization.
b. Gloves. Gloves must be worn when hands may come in contact with human
blood, or OPIM, contaminated items or surfaces. Gloves must be worn
when handling animals that have been inoculated with human cell lines,
human tissue, or infected with HIV, HBV, HCV, or other human pathogens.
Gloves must be replaced as soon as feasible if they are torn or
contaminated. Disposable (single use) gloves must not be washed or
decontaminated for reuse Utility gloves may be decontaminated for reuse,
but must be discarded if they are cracking, peeling or show other signs of
deterioration.
c. Protection for eyes, nose and mouth. Work must be performed in a certified
Biological Safety Cabinet or masks and eye protection (goggles or face
shields) must be worn whenever splash or spray of human blood, or OPIM
to the face is anticipated.
2. Handwashing. Hands must be washed with soap and water after contact
with specimens, as soon as possible after removing PPE and whenever they
become contaminated with human blood, or OPIM. Antiseptic hand cleaner
may be used if soap and water are not available, but hands must be washed
with soap and water as soon as feasible.
3. Personal hygiene. Eating, drinking, smoking, applying cosmetics or lip
balm, or handling contact lenses in the lab is not permitted.
4. Food. Food and drink must not be stored in labs where human blood or
OPIM are present.
5. Pipeting. Mouth pipeting is not permitted.
6. Minimization of aerosols. Splash, spray, spatter, or generation of droplets
must be minimized during any procedure that involves human blood, or OPIM.
If spattering or the generation of aerosols is reasonably anticipated, work
should be performed in a certified Biological Safety Cabinet or eye protection
29
plus a mask or face shield must be worn to prevent an exposure to the mucus
membranes of the eyes, nose and mouth.
7. Sharps handling. Bending, recapping or removing needles is prohibited,
except under specific infrequent circumstances. If recapping, bending or
removing needles or other sharps is required by a specific procedure, and no
alternative is feasible then a one handed scoop technique, mechanical device
or forceps must be used. Written justification supported by reliable evidence
must be included as an addendum to this Exposure Control Plan. This
justification must state the basis for the Principal Investigator’s determination
that no alternative is feasible and must describe the specific procedure that
requires the recapping, bending, or breaking of needles or other sharps.
Disposable sharps must be placed in a plastic sharps container as soon after
use as possible. Approved sharps containers are available from the UVA
Hospital Storeroom. Sharps containers must be easily accessible, with the
opening visible, as close as possible to the area where sharps are used and
maintained upright during use. Sharps containers must be promptly closed,
removed, and replaced when they are ¾ full and placed in a UVA Regulated
Medical Waste Container (CMC) prior to transport for disposal. Broken glass
must not be picked up with bare hands. Reusable sharps, such as surgical
instruments and large bore reusable needles pose the same exposure hazard
as disposable sharps and must be handled in a manner similar to disposable
sharps until they are reprocessed. The container used for temporary storage of
contaminated reusable sharps must be puncture resistant, and labeled as
Biohazard.
8. Safe Medical Devices. Safe medical devices are used to prevent
percutaneous injuries (examples may include needleless devices, shielded
needle devices or plastic capillary tubes). See Appendix B for list of devices
available in the UVA Hospital Storeroom. The Principal Investigator is
responsible for involving employees in the selection of effective engineering
controls and implementing the use of items that would eliminate or minimize
exposures.
9. Specimen Transport on Grounds. For transport to sites within the grounds
of UVA, specimens of human blood and OPIM must be placed in a secondary
leak proof carrier that can contain the contents if the primary container were to
leak or break. Carriers must have the biohazard label affixed to the outer
surface of the transport container.
10. Servicing contaminated equipment. Before servicing or shipping,
contaminated equipment must be decontaminated if possible. If it is not
possible to decontaminate equipment, it must be marked with a biohazard label
describing what parts remain contaminated.
30
11. Central reprocessing of contaminated reusable equipment. Supplies
and equipment returned to a central facility (e.g. department washroom or
autoclave room) for decontamination and reprocessing must be put in a plastic
bag or closeable container and marked with a biohazard label.
12. Housekeeping. The workplace must be maintained in a clean and
sanitary condition. Human blood, or OPIM spills must be cleaned up
immediately with a freshly made 1:10 bleach solution or other approved
disinfectant using appropriate established spill clean up procedures.
Environmental Services is responsible for general cleaning in the majority of
areas at the University and maintains written procedures in their office.
13. Equipment and Working Surfaces. Contaminated work surfaces must be
disinfected with 1:10 freshly made dilution of bleach or an alternative approved
disinfectant.
The decontamination of work surfaces must be done as soon as possible when
contaminated with human blood, or OPIM, after completing procedures or at
the end of the work shift if the surface may have become contaminated since
the last cleaning.
Temporary coverings (plastic backed paper, chux, plastic wrap, foil, etc.) over
bench tops, equipment and other surfaces must be removed and replaced as
soon as possible when contaminated or at the end of the work shift if the
surface may have become contaminated since the last cleaning.
All reusable bins, pails, cans, and similar receptacles which may become
contaminated must be regularly inspected and decontaminated as soon as
possible if they become contaminated.
14. Regulated Medical Waste. Regulated Medical Waste is disposed of by
UVA in accordance with the Virginia Department of Waste Management
Regulations. See the section 5 of the Biosafety Manual for guidance.
IV.
Communication of Hazards to Employees
1. Labels.
Biohazard warning labels must be affixed to refrigerators, freezers,
incubators and other vessels used for storing human blood, or OPIM.
Containers used for transporting human specimens beyond the immediate
work area must have the biohazard label affixed to the outer surface of the
transport container. Biohazard labels are required on areas and equipment
reasonably anticipated to be contaminated with human blood, or OPIM
31
Biohazard labels are not required on individual specimens that are
transported inside a carrier within the University or on Regulated Medical
Waste that has been decontaminated by steam sterilization.
V.
Hepatitis B Vaccination Policy
Employees identified as having potential for exposure to human blood or
OPIM (see II. Exposure Determination), must be offered the Hepatitis B
vaccine at no charge to the employee. The vaccination is a series of three
injections given at approximately 0, 1 and 6 months. A routine booster dose
is not recommended, but will be given at no charge if the U. S. Public Health
Service recommends it in the future.
The vaccine must be offered within 10 working days of initial assignment to
a job category where exposure may occur.
Employees who decline the Hepatitis B vaccine must sign a statement of
declination (page 10). Principal Investigators must keep a copy of the
declination statement on file.
Vl. Procedures for Exposure Incidents.
An exposure is defined as: blood or OPIM contact with broken skin, eyes,
nose, mouth, other mucous membranes, a percutaneous injury with a
contaminated sharp, or contact with blood or OPIM over a large area of
apparently intact skin
In the event of exposure:
1. Wash the area with soap and water or flush eyes, nose or mouth with
large amounts of water for 15 minutes
2. Staff must report immediately to UVA-WorkMed (formerly Occupational
Health) or Employee Health 8am-4:30pm Monday-Friday, or to the
Emergency Room after hours. Students must report to the Student
Health Center 8am-5pm Monday-Friday or to the Emergency Room
after hours.
3. All exposures must be reported to the immediate supervisor and
Principal Investigator.
4. Principal Investigators are responsible for reporting exposure incidents
to the Institutional Biosafety Committee.
32
A. Evaluation and Treatment of Exposures
The evaluation and treatment of an exposure is confidential and will be
given by or under the supervision of a licensed physician and will follow an
established protocol in compliance with OSHA standard 29 CFR
1910.1030, U.S. Public Health Service, CDC guidelines, and Virginia state
law. Evaluation and treatment of exposures are managed by UVAWorkMed, UVA Employee Health or Student Health Services.
If the infectivity status of the source individual is unknown and blood is
available, it will be tested for HIV, hepatitis B and C in accordance with
state law. The exposed employee will be told what the test results are and
what they mean.
If the employee consents, his or her blood will be tested as soon as
possible after exposure to provide baseline hepatitis B, C and HIV status. If
the employee does not consent to HIV testing, the sample will be stored for
90 days and tested if the employee consents in that time period.
Post-exposure prophylaxis will be offered to exposed employees when
medically indicated and as recommended by the US Public Health Service.
Counseling and medical evaluation will be offered for any reported
illnesses the employee develops as a result of the exposure.
B. Documentation of Circumstances Documentation of the circumstances
surrounding the exposure incident is required and allows for the identification
and correction of occupational hazards. Exposure incidents will be investigated
by the Office of Environmental Health and Safety in cooperation with UVAWorkMed.
VIl. Employee Training
All employees who may have the potential for occupational exposure to human
blood, or OPIM must attend a Bloodborne Pathogens training session at the
time of their initial assignment to tasks where occupational exposure may take
place and annually thereafter. Annual retraining is completed via the UVA
OEHS web based training system, or by other OEHS sponsored training
programs. Additional training must be provided whenever there are changes
in tasks or procedures which affect employees' potential for exposure.
33
VIlI. Recordkeeping Procedures
A. Medical Recordkeeping
UVA-WorkMed will establish a medical record for employees who have
exposures. The record will be maintained for the duration of employment plus
30 years. The record is confidential and will not be disclosed to anybody within
or outside the workplace without the employee’s written consent, except as
required by law or regulation.
The record will include: employee name and social security number; dates of
hepatitis B vaccinations and medical records relative to the employee's ability
to receive vaccination; examination results, medical testing, and follow-up
procedures; the healthcare professional's written opinion; information provided
to the healthcare professional who evaluated the employee for suitability to
receive hepatitis B vaccination.
B. Training Records
The Office of Environmental Health and Safety’s web based training system
will track all employees who complete Bloodborne pathogen training.
Employees can access their records through the web based training system.
Records will be maintained for at least 3 years. Employees, employee
representatives, the Commissioner of the Virginia Department of Labor and
Industry and other appropriate agencies may also request copies of these
records.
34
IX. Hepatitis B Vaccination
Employees identified as having potential for exposure to human blood or other
Human-derived materials (see ECP II. Exposure Determination) must be offered
the Hepatitis B vaccine at no charge to the employee. Employees who decline
the Hepatitis B vaccine must sign a statement of declination. Principal
Investigators must keep a copy of the declination statement on file
See scanned document, page 4.
Name
Signature
Date
HepB
vaccine
Yes or
Decline*
*Hepatitis B Vaccine Declination
I understand that due to my occupational exposure to blood or other potentially
infectious materials I may be at risk of acquiring hepatitis B virus (HBV) infection.
I have been given the opportunity to be vaccinated with hepatitis B vaccine, at no
charge to myself. However, I decline hepatitis B vaccination at this time. I
understand that by declining this vaccine I continue to be at risk of acquiring
hepatitis B, a serious disease. If in the future I continue to have occupational
exposure to blood or other potentially infectious materials and I want to be
vaccinated with hepatitis B vaccine, I can receive the vaccination series at no
charge to me.
35
X. Medical Center Storeroom Safety Products (2004)
(This list is updated several times per year as a result of changes in safety items that are
available in the UVA store room. Contact Materials Management if you would like a
current list)
Description of item
Bin #
Catheters
Catheter IV Safety 14G 1 ¼ IN
Catheter IV Safety 16G 1 ¼ IN
Catheter IV Safety 18G 1 ¼ IN
Catheter IV Safety 20G 1 IN
Catheter IV Safety 20G 1 ¼ IN
Catheter IV Safety 22G 1 IN
Catheter IV Safety 24G ¾ IN
97045
97046
97047
97048
97049
97050
91827
Safety needles
Needle Safety Hypo 19GX1.5IN
Needle Safety Hypo 20GX1.5 IN
Needle Safety Hypo 20GX1IN
Needle Safety Hypo 21GX1.5IN
Needle Safety Hypo 23GX1IN
Needle Safety Hypo 25GX5/8IN
91966
91967
91968
91969
91970
91971
Winged Needles
Needle Wing Safety 19Gx7/8 12
Needle Wing Safety 21Gx3/4 3.5
Needle Wing Safety 21Gx3/4 12
Needle Wing Safety 23Gx3/4 12
Needle Wing Safety 23Gx3/4 3.5
Needle Wing Safety 25Gx3/4 3.5
Needle Wing Safety 27Gx3/8 12
92877
92878
92873
92876
92870
92871
92872
Blood Glucose Lancet
Safe-T-Stix Lancets
94002
Safety Syringes
Syringe
10CC 20G
Syringe 3CC 21G 1.5 IN
Syringe 3CC 22G 1 IN
Syringe 3CC 23G 1 IN
Syringe 3CC 25G 5/8 IN
Syringe Safety 3CC w/o needle
Syringe TB 25G 5/8IN
Syringe 3CC w/ vial access cannula
Syringe 10CC w/vial access cannula
90725
90727
90729
90731
90733
97057
90735
92150
92151
36
IV tubing/extension sets/adapters
Adapter Luer Slip
96332
Catheter IV Extension set
96340
Catheter Ext Set Y type 1 valve
96342
Filter Straw
96326
Needle Lock Device 18G
97063
Needleless Injection Cap
97051
Sampling site coupler
96933
Syringe Saline Prefilled 3 cc
96338
Syringe Saline Prefilled 10cc
96345
Tubing IV end cap
91441
Tubing IVAC Pump Primary
93271
Tubing IVAC Pump Blood Set
93274
Tubing IVAC Pump Burette
93271
Tubing IVAC Pump Low Sorbing
99415
Tubing IVAC Pump w/Filter
99416
Tubing Injection Cap
92507
adapt a valve to use with a sharp when appropriate
Tubing Extension Set hi pressure
92875
Tubing IV 2 site 15 drops/ml
93841
Tubing IV 1 site 15 drops/ml
93843
Tubing IV Ext 43IN w/2 Y Inj sites
93844
Tubing IV 3 sites 60 drops/ml
93848
Tubing IV 3 sites 15 drops/ml
93999
Tubing IV Adaptor Vent for unvent bot 99224
Vacutainer Blood Collection Devices:
Plastic Tubes:
3ML Li Heparin PST Green
4ML NaFl K Oxylate Gray
3.5ML SST Gold Tiger
3ML Lavender
7ML Pink Blood Bank
93456
92496
92495
92500
98803
Holder:
Needle Holder Safety
92504
Safety Winged Sets:
Set Safe Lok Blood Collection 21G
Set Safe Lok Blood Collection 23G
Set Safe Lok Blood Collection 25G
92477
92478
92479
Transfer Devices
Vacutainer syringe blood transfer device 90020
Blood Culture Transfer set
96360
Vacutainer IV Tube Holder
94299
Kits
Contact supply room for most current safety kits available.
37
Appendix B Toxin Safety Plan
l. Biotoxin Safety Plan
All Investigators working with toxins of biological origin must complete a
laboratory specific Toxin Safety Plan that includes: toxins used ,know hazards,
safety equipment and protocols, personal protective equipment and training
requirements, toxin inactivation and disposal, inventory control and security
measures and emergency procedures (spills, exposure management)
Guidelines for working with toxins of biological origin can be found in Appendix I
of the CDC/NIH Publication Biosafety in Microbiological and Biomedical
Laboratories and the Toxin Inactivation document that follows.
1. Toxins Used, Total Quantity (maximum amount possessed by Principal
investigator at UVA.)
No toxins used in J.T. Parsons laboratory.
2. Known Hazards (attach MSDS if available) N/A
3. Safety Equipment, Procedures, PPE and Training requirements: N/A
4 Toxin inactivation and Disposal Procedure N/A
5. Inventory Control and Security Procedures: N/A
6. Spill Clean-up and Exposure Management N/A