Institutional Biosafety Committee (IBC)
P.I. Laboratory Self-Audit
(Complete and submit with IBC Protocol Application)
P.I. Name: (Print) __________________________________________________
Date: ___________________ Bldg and Room #: _______________________
Biosafety Level BSL-2 Self-Audit
Yes No N/A
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Work Practices (Standard Microbiological Practices)
Is access to the laboratory limited to laboratory staff or students in the
course when experiments or work with BSL-2 agents is in progress?
Does laboratory staff or students wash hands after handling viable
materials, after removing gloves, and before leaving laboratory?
Are eating, smoking, handling contact lenses, or applying cosmetics
forbidden in the laboratory?
Is food for human use stored outside laboratory work area in refrigerators
designated for this purpose only?
Are only mechanical pipetting devices used? (NO mouth pipetting)
Are sharps placed in red (approved) sharps containers?
Are sharps containers turned in for disposal when 3/4 full?
Are all contaminated cultures, stocks, and other biologically contaminated
waste disposed of as biomedical waste or treated in such a way as to render
them non-biomedical waste?
Are all procedures performed to minimize creation of splashes or aerosols?
Are work surfaces decontaminated with disinfectants that are effective
against the agents of concern on completion of work or at the end of the day
and after any spill or splash of viable material?
Are all infectious liquids disinfected or autoclaved before being poured into
drain?
Are all BSL-2 infected animal carcasses disposed of in an animal carcass
freezer designated for infectious animal carcasses?
Is broken glassware removed by mechanical means (e.g. brush and
dustpan)?
Special Work Practices
Yes No N/A Is the EHS placard posted at entrance(s) to laboratory and a Biohazard sign
posted at a readily visible location within the laboratory?
Yes No N/A Will laboratory personnel work with blood borne pathogens, primates or
dogs?
Yes No N/A If yes, will laboratory personnel be screened for appropriate immunizations,
etc., before working with the blood borne pathogens, primates or dogs?
Yes No N/A Are persons who are at increased risk of acquiring infection, or for whom
infection may have serious consequences, not allowed to enter the
laboratory when work with infectious agents is in progress without
permission of Principal Investigator or instructor?
Yes No N/A Has P.I. or instructor developed a BSL-2 Safety SOP and posted the SOP in
the lab?
Yes No N/A Does Safety SOP establish policies and procedures whereby only persons
who have been advised of the potential hazards and met specific entry
requirements (e.g. immunization) allowed to enter laboratory while
procedures with BSL-2 agents are in progress?
Yes No N/A Are animals not involved in the work being performed prohibited in the
lab?
Yes No N/A Are support personnel and students trained and made aware of hazards and
SOP before working in the laboratory?
Yes No N/A Are spills and accidents that result in overt exposures to infectious materials
immediately reported to the Principal Investigator or instructor?
Yes No N/A Is appropriate medical evaluation provided?
Yes No N/A Is laboratory equipment and work area decontaminated with an effective
disinfectant on a routine basis, or after work with infectious materials is
finished, and especially after overt spills, splashes, or other contamination?
Yes No N/A Is contaminated equipment decontaminated before it is repaired in the
laboratory or sent out of the laboratory for maintenance or other purposes?
Yes No N/A Is personal protective equipment worn as appropriate when working with
viable microorganisms, animals and chemicals?
Yes No N/A
Yes No N/A
Yes No N/A
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Yes No N/A
Yes No N/A
Safety Equipment (Primary Barrier)
Is face protection (goggles, mask, face shield, or other splatter guards) used
for anticipated splashes or sprays of infectious or other hazardous materials
when manipulated outside a biological safety cabinet?
Is protective clothing removed and left in laboratory before going
to non-laboratory areas (cafeteria, library, administrative areas)?
Is protective clothing either disposed of in laboratory or laundered
by institution? (NEVER taken home!)
Does the laboratory perform procedures with a potential for creating
infectious aerosols or splashes or handle high concentrations of infectious
materials?
Does the laboratory have access to a Biosafety Cabinet? (Location: )
a. Is Biosafety Cabinet annual certification current?
b. Is Biosafety Cabinet installed away from doors, high traffic areas,
ventilation devices, or other equipment that could disrupt airflow patterns
needed for containment?
Yes No N/A
Does the laboratory centrifuge high concentrations or large volumes of
infectious agents?
Yes No N/A If yes, does the centrifuge have sealed rotor heads or centrifuge safety cups?
Yes No N/A
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Yes
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No
No
No
No
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N/A
Laboratory Facilities (Secondary Barriers)
Does laboratory have doors that lock?
Does laboratory have a sink for hand washing?
Is laboratory designed so that it can be easily cleaned? (Cloth upholstery,
carpets and rugs are not allowed in laboratory work areas).
Are bench tops impervious to water and resistant to heat, organic solvents,
acids, alkalis, and chemicals used to decontaminate the work surface and
equipment?
Is laboratory furniture and cabinetry in good condition and capable of
supporting loads and uses?
Are spaces between benches, cabinets, and equipment accessible for
cleaning?
Are eyewash/eyewash stations readily available?
Is illumination level adequate?
Is an autoclave readily available and easily accessible?
Is an insect and rodent control program in effect for the laboratory?
Signature of Principal Investigator:___________________________________________
Date: _______________________
Signature of Compliance Officer: __________________________________________
Date: _______________________
(The Compliance Officer will review checklist and ensure that lab meets health and safety
requirements AFTER submission of the project application. Attach the form to your IBC
application packet without the Compliance Officer’s signature.)
Print form and continue to next process >>>