Antibiotics Kanamycin sulphate Hazardous Substances Policy - Assessment Ampicillin CHEMICAL HAZARD AND RISK ASSESSMENT School of Biosciences Name of supervisor Professor GS Besra Assessor Dr Lynn G Dover Assessment Number* 2018 Date of Assessment 16/4/02 Signature Signature Notes A School COSHH form in Word is available on the School Server. Available from the Health and Safety Unit. Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment. Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form. Use a continuation sheet to expand any section of this form in hard copy version. 1 LOCATION OF THE WORK ACTIVITY 2 PERSONS WHO MAY BE AT RISK E114 List names where possible Dr Lynn G Dover Dr William N Maughan Dr Sudagar S Gurcha Dr Petr Illarionov Dr Mark Guy Dr Henri Strating Mr Derek Lonergan Mr Alistair Brown Mr Kevin Gibson Mr Ian Daniels Miss Rosalind Wilson Miss Suzanne Senior Miss Janet Redman Mr Massoud Akkhtar 3 ACTIVITY ASSESSED 4 Growth of bacteria, induction of genes and preparation of cell extracts MATERIALS INVOLVED NAME AMOUNT max/stor ed HAZARD Attach copies of data sheet(s) RISK PHRASES Antibiotics Kanamycin sulphate 25g Harmful R: 61-20/21/22 Ampicillin 100g Irritant R: 42/43 Hygromycin B 25g Irritant Toxic R: 26/27/28-37/38-41 HAZDAT NO*** BIOSCIENCESNO *** Gentamycin sulphate 25g Streptomycin sulphate 25g Irritant Harmful R: 61-36/38-42/43 Irritant Harmful R: 61-20/21/22 Cycloheximide 5g Irritant toxic R: 26/27/28-63-4036/37/38 Isonicotonic acid hydrazide (INH) 50g Irritant Harmful R: 22-36/37/38-40 Ethambutol (EMB) 25g Harmful R: 61 Chloramphenicol 25g Harmful Carcinogen R: 45-46-63-42/43 Cerulenin (CER) 10mg Irritant R: 20/21/22 Ferric ammonium citrate 1Kg Harmful R: 36/37/38 Zinc chloride 500g Irritant R: 34 Zinc sulphate 500g Causes burns R: 36/37/38 Calcium Chloride 1kg Irritant R: 22-36/37/38 Imidazole 1kg Irritant Harmful R: 34-22 Succinic acid 100g Causes burns Harmful R: 36/37/38-41 Ammonium chloride 1kg Irritant R: 22-36 Acetamide 100g Harmful irritant R: 40 Isopropylthiogalactopyran oside (IPTG) 50g Carcinogen Irritant Harmful R: 45-46-20/21/2236/37/38 Sodium hydrogen orthophosphates 1kg Irritant R: 36/37/38 Morpholinoethanesulpho nic acid (MES) 1kg Irritant R: 36/37/38 Buffers & Culture MediumAdditives Morpholinopropanesulph onic acid (MOPS) 1kg Irritant R: 36/37/38 Trizma Base 1kg Irritant R: 36/37/38 Sodium Carbonate 1kg Irritant R: 36 Citric acid 1kg Irritant R: 41-37/38 Sodium Hydroxide 1kg Causes burns R: 35 Potassium hydroxide 1kg Causes burns R: 35 Sodium azide 100g Toxic R: 28-32 Phenylmethylsulphonyl fluoride (PMSF) 1g Toxic Irritant Causes burns R: 23/24/25-34-15 100ml Irreversible effects R: 40 Triton X-100 100ml Irritant R: 22-41 Triton X-114 500ml Irritant R: 36/37/38 Tyloxapol 50g May be harmful to unborn child Irritant R: 61-36/37/38 CHAPS 5g Irritant R: 36/37/38 Igepal C-630 50ml Harmful R: 22-41 Detergents Polyoxyethylenesorbitan monooleate (Tween 80) 5 INTENDED USE** Give brief details and attach protocol/instructions Antibiotics will be added to culture media at low (g/ml) in order to select for recombinant bacteria. Detergents will be used to solubilise membrane extracts. 6 RISKS to HEALTH and SAFETY from INTENDED USE From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially at risk? Kanamycin sulphate, gentamycin sulphate, streptomycin sulphate, EMB & Tyloxapol may all be harmful to unborn children and should be avoided by pregnant workers. Materials that cause burns, i.e. Zinc salts and PMSF are to be used in small amounts (mg) and therefore wearing of latex gloves and a laboratory coat should provide adequate protection. 7 CONCLUSIONS ABOUT RISKS Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary? Acceptable level of risk. Cannot substitute chemicals. See below for control measures 8 CONTROL MEASURES Additional to Good Chemical Practice Wear latex gloves for handling all listed chemicals. Face masks should be worn by those who suspect may be pregnant while dispensing Kanamycin sulphate, gentamycin sulphate, streptomycin sulphate & EMB Tyloxapol boils at 68C so should be sterilised by filtration rather than in an autoclave. Materials that cause burns, i.e. Zinc salts and PMSF are to be used in small amounts (mg) and therefore wearing of latex gloves and a laboratory coat should provide adequate protection. 9 INSTRUCTION/TRAINING Specify course(s) and/or special arrangements. New staff/students to be trained by postdoctoral fellows where applicable. 10 MONITORING Performance of control measures, Personal exposure Health Surveillance Personal exposure should not occur if the control measures are observed. As Personal exposure. 11 WASTE DISPOSAL PROCEDURE See School Server for Approved Procedure Document on specific Chemical Waste Disposal. Spent culture medium and other contaminated fluids are to be autoclaved and disposed of via normal route 12 REVIEW Enter the date or circumstances for review of assessment (maximum review interval 5 years) Update names of users as changes occur 13 EMERGENCY ACTION TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc. Disinfect spillages of biological materials with Microsol3. Allow at least 5 min contact time for disinfection and then clear up spill using absorbent material. Autoclave absorbents and dispose of as solid waste. Wash area thoroughly. Non-contaminated fluids should be absorbed and the area washed thoroughly. TO PROTECT PERSONNEL Evacuation, protection for personnel involved in clean-up, Special First Aid Inform other laboratory members to avoid the affected area during cleaning Those involved in cleaning should wear latex gloves. Face masks should be worn when clearing up gram-scale spills of Zn2+ salt and antibiotics powders. Spills should be contained using absorbent granules and then placed in a closed container and arrange for its disposal. TO RENDER SITE OF EMERGENCY SAFE See control hazards and personnel protection CONTACT Clean-up/decontamination Professor GS Besra Prof G.S.Besra 51825 PHONEExt. 51825 10.10.00 * ** *** Prefix T is used for Teaching Assessment Number. Please include amount of chemicals used and how. Hazdat No is the UNICOSHH datasheet report number. Biosciences No is the Biosciences data sheet number. UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER. BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.