Growth of bacteria, induction of genes and preparation of cell extracts

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Antibiotics
Kanamycin sulphate
Hazardous Substances Policy - Assessment
Ampicillin
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
Professor GS Besra
Assessor
Dr Lynn G Dover
Assessment Number*
2018
Date of Assessment
16/4/02
Signature
Signature
Notes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
E114
List names where possible
Dr Lynn G Dover
Dr William N Maughan
Dr Sudagar S Gurcha
Dr Petr Illarionov
Dr Mark Guy
Dr Henri Strating
Mr Derek Lonergan
Mr Alistair Brown
Mr Kevin Gibson
Mr Ian Daniels
Miss Rosalind Wilson
Miss Suzanne Senior
Miss Janet Redman
Mr Massoud Akkhtar
3
ACTIVITY ASSESSED
4
Growth of bacteria, induction of genes and preparation of cell extracts
MATERIALS
INVOLVED
NAME
AMOUNT
max/stor
ed
HAZARD
Attach copies of data sheet(s)
RISK PHRASES
Antibiotics
Kanamycin sulphate
25g
Harmful
R: 61-20/21/22
Ampicillin
100g
Irritant
R: 42/43
Hygromycin B
25g
Irritant
Toxic
R: 26/27/28-37/38-41
HAZDAT NO***
BIOSCIENCESNO
***
Gentamycin sulphate
25g
Streptomycin sulphate
25g
Irritant
Harmful
R: 61-36/38-42/43
Irritant
Harmful
R: 61-20/21/22
Cycloheximide
5g
Irritant
toxic
R: 26/27/28-63-4036/37/38
Isonicotonic acid
hydrazide (INH)
50g
Irritant
Harmful
R: 22-36/37/38-40
Ethambutol (EMB)
25g
Harmful
R: 61
Chloramphenicol
25g
Harmful
Carcinogen
R: 45-46-63-42/43
Cerulenin (CER)
10mg
Irritant
R: 20/21/22
Ferric ammonium citrate
1Kg
Harmful
R: 36/37/38
Zinc chloride
500g
Irritant
R: 34
Zinc sulphate
500g
Causes burns
R: 36/37/38
Calcium Chloride
1kg
Irritant
R: 22-36/37/38
Imidazole
1kg
Irritant
Harmful
R: 34-22
Succinic acid
100g
Causes burns
Harmful
R: 36/37/38-41
Ammonium chloride
1kg
Irritant
R: 22-36
Acetamide
100g
Harmful
irritant
R: 40
Isopropylthiogalactopyran
oside (IPTG)
50g
Carcinogen
Irritant
Harmful
R: 45-46-20/21/2236/37/38
Sodium hydrogen
orthophosphates
1kg
Irritant
R: 36/37/38
Morpholinoethanesulpho
nic acid (MES)
1kg
Irritant
R: 36/37/38
Buffers & Culture
MediumAdditives
Morpholinopropanesulph
onic acid (MOPS)
1kg
Irritant
R: 36/37/38
Trizma Base
1kg
Irritant
R: 36/37/38
Sodium Carbonate
1kg
Irritant
R: 36
Citric acid
1kg
Irritant
R: 41-37/38
Sodium Hydroxide
1kg
Causes burns
R: 35
Potassium hydroxide
1kg
Causes burns
R: 35
Sodium azide
100g
Toxic
R: 28-32
Phenylmethylsulphonyl
fluoride (PMSF)
1g
Toxic
Irritant
Causes burns
R: 23/24/25-34-15
100ml
Irreversible effects
R: 40
Triton X-100
100ml
Irritant
R: 22-41
Triton X-114
500ml
Irritant
R: 36/37/38
Tyloxapol
50g
May be harmful to
unborn child
Irritant
R: 61-36/37/38
CHAPS
5g
Irritant
R: 36/37/38
Igepal C-630
50ml
Harmful
R: 22-41
Detergents
Polyoxyethylenesorbitan
monooleate
(Tween 80)
5
INTENDED USE**
Give brief details and attach protocol/instructions
Antibiotics will be added to culture media at low (g/ml) in order to select for recombinant bacteria.
Detergents will be used to solubilise membrane extracts.
6
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
Kanamycin sulphate, gentamycin sulphate, streptomycin sulphate, EMB & Tyloxapol may all be harmful to unborn
children and should be avoided by pregnant workers.
Materials that cause burns, i.e. Zinc salts and PMSF are to be used in small amounts (mg) and therefore wearing
of latex gloves and a laboratory coat should provide adequate protection.
7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
Acceptable level of risk. Cannot substitute chemicals. See below for control measures
8
CONTROL MEASURES
Additional to Good Chemical Practice
Wear latex gloves for handling all listed chemicals.
Face masks should be worn by those who suspect may be pregnant while dispensing Kanamycin sulphate,
gentamycin sulphate, streptomycin sulphate & EMB
Tyloxapol boils at 68C so should be sterilised by filtration rather than in an autoclave.
Materials that cause burns, i.e. Zinc salts and PMSF are to be used in small amounts (mg) and therefore wearing
of latex gloves and a laboratory coat should provide adequate protection.
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
New staff/students to be trained by postdoctoral fellows where applicable.
10
MONITORING
Performance of control measures,
Personal exposure
Health Surveillance
Personal exposure should not occur if the control
measures are observed.
As Personal exposure.
11
WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Spent culture medium and other contaminated fluids are to be autoclaved and disposed of via normal route
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
Update names of users as changes occur
13
EMERGENCY ACTION
TO CONTROL HAZARDS
To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Disinfect spillages of biological materials with Microsol3. Allow at least 5 min contact time for disinfection and
then clear up spill using absorbent material. Autoclave absorbents and dispose of as solid waste. Wash area
thoroughly. Non-contaminated fluids should be absorbed and the area washed thoroughly.
TO PROTECT PERSONNEL
Evacuation, protection for personnel involved in clean-up, Special First Aid
Inform other laboratory members to avoid the affected area during cleaning
Those involved in cleaning should wear latex gloves.
Face masks should be worn when clearing up gram-scale spills of Zn2+ salt and antibiotics powders. Spills
should be contained using absorbent granules and then placed in a closed container and arrange for its disposal.
TO RENDER SITE OF EMERGENCY SAFE
See control hazards and personnel protection
CONTACT
Clean-up/decontamination
Professor GS Besra
Prof G.S.Besra
51825
PHONEExt.
51825
10.10.00
*
**
***
Prefix T is used for Teaching Assessment Number.
Please include amount of chemicals used and how.
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.
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