HSC CHEMISTRY PROGRAM MODULE: WEEK 1 OPTION 3 FORENSIC CHEMISTRY SYLLABUS OUTCOMES TEACHING STRATEGIES & ACTIVITIES Jacaranda Chemistry 2 Page Reference The identification of inorganic and organic substances The forensic chemist Chapter 19 1. Use a table to summarise the issues related to accuracy in analysis and avoiding contamination of 1. Outline precautions that may be necessary to forensic samples. ensure accuracy and prevent contamination of samples for analysis 2. Use flow charts or tables to distinguish between 2. Distinguish between organic and inorganic inorganic and organic compounds. compounds 3. Use structural formulae and models to 3. Explain that there are different classes of distinguish between hydrocarbons , alkanols and carbon compounds including: alkanoic acids. hydrocarbons (b) alkanols (c) alkanoic 4. Examine various soil samples using a acids , which can be identified by microscope. Summarise gathered data on the distinguishing tests properties of soil that are useful for a forensic 4. Explain that the inorganic chemical properties chemist. of soils and other materials may be useful 5. Discuss the use of new technologies evidence (chromatography/ DNA profiling) to examine case 5. Discuss, using a recent example, how progress in analytical chemistry and changes in studies such as the Rainbow Warrior and the attack on the World Trade Centre. technology can alter the outcome of a forensic Sign The forensic chemist 1. 549-554 2. 555 3. 556-558 4. 558- 561 5. 551-552 553-554 investigation 1 6. Solve problems and use available evidence to discuss the importance of accuracy in forensic chemistry 7. Solve problems and use available evidence to discuss ethical issues that may need to be addressed during an analytical investigation 8. Identify data, plan and perform first-hand investigations to determine a sequence of tests to distinguish between organic and inorganic compounds 9. Gather and process information from secondary sources to present information summarising a series of distinguishing tests to separate: (a) the groups of hydrocarbons (b)acids, bases and neutral salts ,in the school laboratory and in the forensic chemist’s laboratory 2 Analysis of carbohydrates 10. Identify that carbohydrates are composed of carbon, hydrogen and oxygen according to the formula : Cx (H2O)y 11. Identify glucose as a monomer and describe the condensation reactions which produce: (a) sucrose as an example of a disaccharide (b) polysaccharides including glycogen, starch and cellulose 6. Solve problems and discuss issues related to accuracy in forensic investigations. 7. Discuss the ethical issues that need to be addressed in forensic investigations 8. PRACTICAL ACTIVITY 19.1Distinguishing tests for common inorganic and organic compounds. 9. DATA ANALYSIS 19.7 - Comparison of tests for selected organic and inorganic substances in the school laboratory and the forensic laboratory. 6. 549-550 7. 550-551 8. 593 9. 600-603 Revision Questions - Jacaranda Chemistry 2. Set Revision 19.1 Pages 561-562 19.1 Analysis of carbohydrates 10. Use models or structural formulae to verify that carbohydrates can be represented by the formula: Cx (H2O)y 11. Use diagrams and models to identify monosaccharides, disaccharides and polysaccharides. Identify glucose as the monomer in starch and cellulose. Chapter 19 10. 563 11. 563- 569 2 12. Describe the chemical difference between reducing and non-reducing sugars 13. Distinguish between plant and animal carbohydrates’ composition in terms of the presence of: (a)cellulose (b)starch 3 12. Compare the ring and open chain structure of glucose and sucrose and explain why only glucose is a reducing sugar. 13. Use diagrams to distinguish between cellulose, starch and glycogen (c)glycogen 14. PRACTICAL ACTIVITY 19.2(a)- Testing 14. Choose equipment, plan and perform a for reducing and non-reducing sugars and first-hand investigation to carry out a series starch of distinguishing tests for the carbohydrates: 15. PRACTICAL ACTIVITY 19.2(b)- Modelling (a) reducing and non-reducing sugars monosaccharides and starch (b)starch 15. Use available evidence and perform first-hand investigations using molecular model kits, computer simulations or other multimedia resources to compare the Revision Questions - Jacaranda Chemistry 2. Set structures of organic compounds including: 19.2 (a) monosaccharides (b) starch 12. 569-571 Analysis of proteins Chapter 19 16. 577-579 16. Distinguish between protein used for structural purposes and the uses of proteins as enzymes 17. Identify the major functional groups in an amino acid 18. Describe the composition and general formula for amino acids and explain that proteins are chains of amino acids 19. Describe the nature of the peptide bond and explain that proteins can be broken at different lengths in the chain by choice of enzyme Analysis of proteins 16. Compare , with the aid of diagrams, structural protein and globular protein. 17. Use diagrams and models to identify the functional groups in an amino acid. 18. Use tabulated information to compare the structures of various amino acids. Identify proteins as polypeptides. 19. Draw diagrams and construct models of the peptide bond. Solve problems related to the cleavage of the protein chain by selected enzymes. 13. 566-569 14. 594 15. 594-595 Revision 19.2 Pages 572-574 17. 574 18. 575-576 19. 579-582 3 4 20. Compare the processes of chromatography and electrophoresis and identify the properties of mixtures that allow them to be separated by either of these processes 21. Discuss the role of electrophoresis in identifying the origins of protein and explain how this could assist the forensic chemist 22. Perform first-hand investigations using molecular model kits, computer simulations or other multimedia resources to present information which describes the composition and generalised structure of proteins 20. Use gathered data to compare and contrast chromatography and electrophoresis. Discuss the properties of amino acids and proteins that allow separation by either procedure. 21. Explain how forensic chemists utilise electrophoresis in investigations 22. PRACTICAL ACTIVITY 19.3- Modelling the structure of proteins 20. 582- 588 Analysis of proteins Analysis of proteins Chapter 19 23. Perform a first-hand investigation and gather 23. Discuss the chemical basis of the Biuret test and first-hand information about a distinguishing the ninhydrin test for amino acids and proteins. test for proteins PRACTICAL ACTIVITY 19.4- Distinguishing tests for proteins 24. Discuss the chromatographic procedure used to separate leaf pigments. 25. Perform a first-hand investigation and gather 25. PRACTICAL ACTIVITY 19.5first-hand information to identify the range of Chromatography: selecting solvents to extract solvents that may be used for chromatography and suggest mixtures that may be separated and pigments from plant leaves. identified by the use of these solvents 26. Describe the process of paper and gel electrophoresis of proteins and amino acids 26. Perform a first-hand investigation to carry PRACTICAL ACTIVITY 19.6 out the electrophoresis of an appropriate Electrophoresis simulations and animations mixture and use available evidence to identify the characteristics of the mixture which allow it Revision Questions - Jacaranda Chemistry 2. Set to be separated by this process. 19.3 24. Perform a first-hand investigation to carry out chromatography to separate a mixture of organic materials such as the pigments in plants 21. 587-588 22. 595-596 23. 579, 596 24. 583-584 25. 597-598 26. 587- 588 598-599 Revision 19.3 Pages 589-590 4 5 Instrumental Methods in Forensic Chemistry DNA and forensic chemistry 27. Outline the structure and composition of DNA 28. Explain why analysis of DNA allows identification of individuals 29.Describe the process used to analyse DNA and account for its use in: (a) identifying relationships between people (b) identifying individuals 30.Analyse information to discuss the range of uses of DNA analysis in forensic chemistry and use available evidence in discussing the issues associated with its use in terms of the ethics of maintenance of data banks of DNA Sensitive analytical techniques 31. Explain what is meant by the destructive testing of material and explain why this may be a problem in forensic investigations 32. Identify, outline and assess the value of the following techniques in the analysis of small samples: (a) gas-liquid chromatography (b) high performance liquid DNA and forensic chemistry Chapter 20 27. Use photos, models and drawings to analyse the structure of DNA in terms of its phosphate, sugar and nitrogen bases. 28. Explain the unique nature of DNA to each individual in terms of exons and introns. 29. Describe DNA profiling and use diagrams to summarise the steps in creating a DNA profile. 30. Summarise the uses of DNA profiling and discuss the ethical issues involved in maintaining DNA databanks. 27. 605- 608 28. 608 29. 608- 614 30. 614-617 Revision Questions - Jacaranda Chemistry 2. Set Revision 20.1 Pages 617-618 20.1 Sensitive Analytical Techniques 31. Describe examples of destructive and nondestructive forensic testing. 31. 619- 620 32. 620-625 32. Use diagrams of GLC and HPLC equipment to explain how these techniques can be used to analyse small samples of forensic materials. chromatography 5 6 Sensitive Analytical techniques 33. Outline how a mass spectrometer operates and clarify its use for forensic chemists 34. Analyse and present information from secondary sources to discuss the ways in which analytical techniques may provide evidence about samples Sensitive Analytical Techniques 33. Use a diagram of a mass spectrometer to explain how it operates. Explain how it can be used in forensic analysis. 34. Use secondary sources to analyse examples of how analytical techniques can be used by forensic chemists. Revision Questions - Jacaranda Chemistry 2. Set 20.2 Chapter 20 33. 626- 629 34. 629-633 Revision 20.2 Pages 633-637 Emission spectra and forensic chemistry Emission spectra and forensic chemistry 35. Describe the conditions under which atoms will emit light 36. Identify that the emission of quanta of energy as electrons move to lower energy levels may be detected by humans as a specific colour 37. Explain why excited atoms in the gas phase emit or absorb only certain wavelengths of light 38. Account for the fact that each element produces its signature line emission spectrum 35. Demonstrate the emission of light when salts 35. 637- 639 are vapourised in a Bunsen flame. Describe the conditions that lead to light emission. 36/37 639- 641 36. Identify using diagrams that electrons exist in quantised energy levels. Explain how particular frequencies of light are related to quantised energy 38. 641 gaps using Planck's equation. 37. Use Bohr's theory to explain how light photons can be emitted from excited atoms. 38. Use energy level diagrams to explain the unique nature of the spectra of each element. 6 7 Emission spectra and forensic chemistry 39. Discuss the use of line emission spectra to identify the presence of elements in chemicals 40. Identify data, choose equipment, plan, and perform a first-hand investigation using flame tests and/or spectroscope analysis as appropriate to identify and gather first-hand information to describe the emission spectrum of a range of elements including Na and Hg 8 41. Process and present information from secondary sources to analyse and identify individual elements present in a mixed emission spectrum and use available evidence to explain how such information can assist analysis of the origins of a mixture GENERAL REVISION Emission spectra and forensic chemistry 39. Describe the principle of atomic emission spectroscopy and explain how it is used to identify elements in chemicals. Chapter 20 39. 641- 644 40. PRACTICAL ACTIVITY 20.1 - Observing emission spectra 40. 650 - 651 41. DATA ANALYSIS 20.2 - Identifying elements using emission spectra 41. 644 652 Revision Questions - Jacaranda Chemistry 2. Set Revision 20.3 Pages 644-647 20.3 CD- MODULE Option 3 - REVISION QUESTIONS - Chapters 19 and 20 CD Option Module 3 - REVISION QUESTIONS Use the supplied set of questions to revise all of Option Module 3 20 Multiple Choice and 20 open-ended questions in the style of the HSC are available. Model answers are available on the CD. 7