Veterinary Investigation and Testing in Reptiles During investigation for the cause of disease in reptiles (including snakes, lizards and tortoises/terrapins) diagnostic tests are commonly used. Tests tend to be performed more frequently than in dogs and cats for several very important reasons. Reptiles are much more complex in terms of their anatomy/biology than mammals (such as dogs, cats and rabbits) and can be affected by very different diseases. Most diseases cannot be diagnosed by examination alone, obviously we cannot see what is going on inside them just be looking and feeling. Some infectious diseases pose a serious risk to human health and it is important that we test for these for your safety. As they are very good at hiding illness, many reptiles are severely ill by the time they are brought to us. We do not have the luxury of trying a series of treatments to see how they respond as many will die before the correct treatment is found. Testing is the fastest way for us to select the appropriate treatment and maximize their chances of survival. It is important to note that due to this, some animals will die during the course of treatment even when testing has been performed. This does not mean the test was a waste of time, it just means that the animal was so sick when it was examined that its chances of survival were very low to begin with. X-Rays (Radiography) We use these to look inside the body to see if there are any changes from normal which is vital to assess bone density, look for eggs and identify abdominal blockages amongst other problems. This is one of the best tests to diagnose metabolic problems in these animals which are an extremely common problem. Please be aware that not all problems can be diagnosed using this technique and we may need to combine this with other tests. Testing for Viral Infections This may be done through collecting blood samples, faecal testing or saliva testing. We can test for some viruses if we suspect these are the cause of an infection. It is very important to do this in some cases, especially if owners have a large collection of reptiles as some viruses can spread very quickly and may even result in the death of several animals. They can also have an impact on the reproductive success of collections. If we think your pet has a virus we WILL NOT prescribe antibiotics unless there is evidence of a bacterial infection as well. This is because antibiotics do not have any effect on viruses. This is why doctors do not give you antibiotics for a cold. Testing for Bacterial Infections Animals can have infections caused by bacteria. We send a sample to a lab and ask them to find out what the bacteria is and the best antibiotic to treat it with. In reptiles, it is common to have more than one type of bacteria present in an infection and they are often resistant to several antibiotics (i.e. the antibiotics will not work against them). An example of the information we obtain is recorded below: Village Vets Centre Ltd 2014 Page 1 Breed: ROYAL PYTHON Culture: Aerobic cultures show a moderate pure growth of 1. Pseudomonas aeruginosa Anaerobic Culture POSITIVE 2. Clostridium species isolated SENSITIVITIES: 1 2 Augmentin RESISTANT Sensitive Ceftazidime Sensitive RESISTANT Gentamicin Sensitive RESISTANT Amikacin Sensitive RESISTANT Enrofloxacin RESISTANT Sensitive Marbofloxacin RESISTANT Sensitive Doxycycline RESISTANT Sensitive Metronidazole RESISTANT Sensitive This snake had infections with two different bacteria meaning two different antibiotics were needed. One bacteria (Pseudomonas) was resistant to 5/8 antibiotics and could not be treated by using them. Sometimes the lab cannot find anything and the test needs to be repeated. Simply trying an antibiotic to see what happens is not the best thing to do. It encourages antibiotic resistance to develop and a delay in selecting the right drug can be fatal. The above example shows that in some cases we are more likely to select the wrong drug than the right one for the infection if we do not test to find out which bacteria is the cause of the infection. There are in fact hundreds of different bacteria that can cause infection and there is no point us trying to guess which one is the cause as they are all very different. Testing Faeces (Droppings) for Intestinal Parasites To get accurate results it is important to collect droppings over a few days and not let them get too dry as parasites may not be shed in the droppings every day. It is often easier for owners to send the faeces direct to the laboratory we use (PALS Veterinary Laboratories) in order to be tested. The most useful test to select is ‘Reptile Faeces 2’ as it will cover both parasites (e.g. worms & Coccidia) and bacteria (e.g. Salmonella and Campylobacter). This is not the cheapest test but it is by far the most useful. A copy of a report is below: MICROBIOLOGY: Bearded Dragon droppings Direct Smear: Cryptosporidium: SCANTY COCCIDIA AND NEMATODE OVA SEEN Negative Yeast: ++ What does it mean? This lizard has coccidiosis (a gut parasite) and needs treatment. It has intestinal worms (nematodes) and needs treatment. Village Vets Centre Ltd 2014 Page 2 It does not have cryptosporidia. (a serious intestinal infection). It has yeast in its intestines and needs treatment. The underlying reason for this is normally that the vivarium is not warm enough. In this case, the lizard needs three different medications: one to treat the coccidian parasite, one for the worms, and one for the yeast. More heat must also be provided. Sometimes more than one type of worm is present and two different wormers may be needed. It is very important to test the faeces of all new reptiles brought in to a collection as you do not know if they are infected just by looking – they can appear healthy but be riddled with disease. Shops very rarely mention this. Blood testing Blood tests are used to look for problems with the internal organs (such as the liver and kidneys). The animal can be checked for things such as anaemia (not enough red blood cells) or changes with the white blood cells which can tell us useful information. These tests often need to be repeated during treatment to check that it is working and to monitor for side effects of certain drugs. Limitations of Testing Clients will sometimes ask if there is a guarantee that their animal will get better before they pay for tests. Unfortunately, there are no guarantees as we are dealing with biological systems which work every differently. We cannot guarantee a diagnosis will be reached even after a test is performed as no test is 100% perfect and it is the same in human medicine. Please note: Diagnostic tests are NOT a treatment in themselves. They enable us to select the correct treatment to use and your pet will not get better without completing the treatment course. Testing will at least let us know what is not wrong with your animal, whether it is worth trying to treat the animal at all or if there is a risk to the owners and in fact euthanasia is the correct thing to do. We only use veterinary laboratories based in the UK. This is so that in the event of a dispute involving court we can call on the laboratory to appear in court. Pricing We routinely give clients estimates for any work to be done, it must be remembered that these are estimates and by their very nature can increase or decrease depending upon the circumstances. We will offer every client the ideal set of tests for a suspected problem which can include any combination of x-rays, blood tests and faecal tests etc. We realise that people have different budgets so we will also give you estimates which involve less testing which will mean lower estimates but doing so may mean we get less accurate or helpful results as an element of guess work is then involved in treatment. Your range of estimates may go from £200+ with investigations and testing, to £45 if we just see how the animal responds to treatment. In some cases tests will also need to be repeated and both sets of tests will need to be paid for. As we rely heavily on the NHS for medical treatment, people are not used to paying for medical costs and it can come as a surprise. There is no animal NHS so owners are entirely responsible for paying fees. You will be charged fees for the laboratory to do the work plus a collection and interpretation fee for the results as you are paying for our time to advise you on the best treatment course. If you send samples directly to the laboratory you will still be charged an interpretation fee when the results are sent to us. For more information visit: www.village-vets.com/client-info/laboratory-tests/ www.palsvetlab.co.uk Village Vets Centre Ltd 2014 Page 3