Supporting Information:
Hyperbranched Polymers as Delivery Vectors for
Oligonucleotides
Jian Hong Tan1, Nigel A. J. McMillan2, Elizabeth Payne2, Cameron Alexander3, Felicity
Heath3,
Andrew K Whittaker1 and Kristofer J Thurecht1*
1
Australian Institute for Bioengineering and Nanotechnology and Centre for Advance
Imaging, The University of Queensland, St Lucia, Queensland 4072, Australia.
2
The University of Queensland Diamantina Institute, The University of Queensland,
Research Wing, Building 1 Princess Alexandra Hospital, Woolloongabba, Queensland,
4102, Australia.
3
School of Pharmacy, The University of Nottingham, Nottingham, NG7 2RD, United
Kingdom
k.thurecht@uq.edu.au
10 100 200 10 100 200 10 100 200
M O N- N
P1
P2
P3
Figure S1. PAGE analysis showing stability of 9-27 oligo:polymer complexes in presence of
DNAse. Exposed electrophoresis gel shows molecular weight of free 9-27 oligo in absence of
DNAse for reference (N-), and then molecular weight following exposure to DNAse (37 oC,
pH 5.5 for 10 minutes) of 9-27 oligo in absence of polymer (N), and 9-27 oligo complexed
with oligofectamine (O) and hyperbranched polymers (P1, P2, P3). Complexes are referenced
to a standard 1kbp DNA ladder up (M). The N:P ratio for the hyperbranched polymers are
shown at the top of the figure. In all cases, the polymer complexes sufficiently protected the
ODN from enzymatic degradation.
Figure S2. Atomic force micrograph of P3 complexed to oligoDNA at N:P of 1. Similar
binding properties are observed as that reported for P1 (Figure 6).