طقف يمسرلا مادختسلا
ثحبلا مقر
ثحبلا لاجم
:ةيلكلا مسا
فئاطلا ةعماج – بطلا ةي لك
:
ةيبطلا ماسقلأا
ةيبطلا ةيويحلا ءايميكلا مسقو ةعانملاو ةقيقدلا ءايحلأا مسق
: ثحبلا ءدب خ يرات
ةقفاوملاو دقعلا عيقوت ةيادب
تاظحلام
: ةحرتقملا ثحبلا ةدم
رهش 12
: بولطملا يلا م جلإا غلبملا
ريال
146000
ةيسنجلا
ثحابلا اهب لمعي يتلا ةهجلا مسا
)ةيلكلا / مسقلا(
بقللا
يملعلا
نيثحابلا ءامسأ
ىرصم بطلا ةيلك – ةعانملاو ةقيقد لا ءايحلأا
ذاتسأ
كراشم
يرصم
يرصم
يرصم
بطلا ةيلك – ةيبطلا ةيويحلا ءايميكلا
مولعلا ةيلك – ءايحلأا
بطلا ةيلك – ةعانملاو ةقيقدلا ءايحلأا
ةيلكلا ديمع عيقوت
ذاتسأ
كراشم
ذاتسأ
كراشم
ذاتسأ
دعاسم
ىسيئرلا ثحابلا
دعسلاوبأ باهولادبع رباص زيزعلادبع.د
:) 1 ( مقر كراشم ثحاب
باهش ةعمج دمحم رباج /د
:) 2 ( مقر كراشم ثحاب
نسح ميحرلادبع حلا ص ناميا .د
:) 3 ( مقر كراشم ثحاب
نيضوع ميهاربا دمحم ىمزع دامع .د
يسيئرلا ثحابلا عيقوت
1

Official Use
Project NO.
Field:
N.B
Title of the Project:
Modulatory roles and anticonvulsant activities of some natural products against Pilocarpine-induced seizures in mice
Starting Date
(after final approval and signing contract)
Name
Principle Investigator:
Dr/ Abdelaziz S. A. Abuelsaad
Duration
(12 Months)
Total Fund
146000 SR
Degree Department/College
Associate
Professor
Microbiology and Immunology/
College of Medicine
Nationality
Egyptian
Co-Investigator-1:
Dr. Gaber Mohamed Gomaa Shehab
Associate
Professor
Co-Investigator-2:
Dr. Eman Salah Abdelreheem Hassan
Associate
Professor
Medical Biochemistry/
College of Medicine
Biology/
Faculty of Sciences
Egyptian
Egyptian
Co-Investigator-3:
Dr. Imad Azmi M. I Awadain
Assistant
Professor
Microbiology & Immunology/
College of Medicine
Egyptian
Dean Principle Investigator
2

English contents
Content
يبرعلا ىوتحملا No
1 Arabic Cover page
2 English Cover page
3 Arabic Abstract
4 English Abstract
5 Introduction
6 Literature Review
7 Aim of study
8 Research Design and Methods
9 Management Plan
10 The net gain from the project
11 References
12 Financial fund distribution
13 List of requested chemicals
14 Principal Investigator CV
16 Co-1 Investigator CV
17 Co-2 Investigator CV
18 Co-3 Investigator CV
19 Reviewer-1
20 Reviewer-2
21 Reviewer-3
22 Reviewer-4
23 Reviewer-5
ةيبرعلا ةغللاب فلاغ
ةيزيلجنلإا ةغللاب فلاغ
يبرعلا صخلملا
ىزيلجنلإا صخلملا
ةمدقملا
ثحبلا تايبد أ
ةساردلا فادهأ
هقرطو ثحبلا ميمصت
لمعلا ةطخ
ثحبلل ةيئاهنلا ةلصحملا
عجارملا
ثحبلا عورشم ةينازيم صخلم
تاديوزتلاو تايوايميكلاب ةمئاق
يسيئرلا ثحابلل ةيتاذلا ةريسلا
1 كراشملا ثحابلل ةيتاذلا ةريسلا
2 كراشملا ثحابلل ةيتاذلا ةريسلا
3 كراشملا ثحابلل ةيتاذلا ةريسلا
1 مكحم تانايب
2 مكحم تانايب
3 مكحم تانايب
4 مكحم تانايب
5 مكحم تانايب
ص
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دعسلاوبأ باهولادبع رباص زيزعلادبع .د : يسيئرلا ثحابلا – 2
ةيبطلا مولعلاو بطلا : ةيلكلا مسأ 3
ةعانملاو ةقيقدلا ءايحلأا مسق : مسقلا مسأ 4
هل براجتلا تاناويح جذومن ىف عرصلا ضرمل ببسملا
ىف ةيضرم تاريغت ىف ببستي امم ةدراشلا تا
نيبراكوليابلا نأ ةثيدحلا تاساردلا تتبثأ دقل
نويلأا نم ةلئاه ةيمك جاتناو ىبصعلا زاهجلا ىلع ةيمس تاريثأت
طبثملا ريثأتلاو تاجنشتلاو عرصلل داضملا ريثأتلاو ىعانملا ليدعتلا مييقت ىلإ ةيلاحلا ةساردلا فدهتو .خملا ةجسنأ
لحارمروطت ةسارد ) 1 ( :ةيلاتلا فادهلأا قيقحت ةساردلا صلختو .ةيعيبطلا تاتابنلا تاصلختسم ضعبل ةدسكلأل
ايلاخب ةقاطلا ىوتسمو ضرملا راشتنا ىلع ةدراشلا ةدسكلأا تانويأ تاريثأت مييقتب تاجنشتلاو عرصلا ضرم
نم جلاع ريوطت ةلواحم ) 2 ( ,ةيئايميكلا ةيبصعلا لاّيسلا تلاقان ىوتسمو
تابنل يساسلأا بكرملا تاريث
باهتللإا تانيكوتياس ىوتسمو خملا
أت ةسارد لثم براجتلا نارئف نم جذومن ىف تاجنشتلل داضمك ةيعيبطلا تاجتنملا
تاصلختسملا دحأك كيجلايلإا ضمحو نديريبيسيهلا تابكرم ةسارد ىلع ةولاع )نونيكوميثلا( ةكربلا ةبح
ةساردلا فدهتو .ةيعرصلا تاجنشتلا ضرم جلاع ىف ايبط مدختسملا تاريميبوت راقعلا عم لمكم جلاعك ةيعيبطلا
ةباصلإا دنع طشنت ىتلا ةينومرهلا ددغ ل ل ةيجولويبلا فئاظولا ىلع ةيع يبطلا تاجتنملل داضملا ريثأتلا ةفرعم ىلع
.
عرصلا و تاجنشتلا ضرمب
4

: ةيلاتلا رصانعلا ىلع ثحبلا عورشم يوتحي نأ بجي
.فلاغلا ةحفص
.ثحبلا حرتقم تايوتحم ةمئاق
)ةملك 200 نع ديزت لا( .)يبرع( ثحبلا صخلم
)ةملك 200 نع ديزت لا( .)يزيلجنإ( ثحبلا صخلم
.ثحبلا تايبدأ ضارعتسا
.ثحبلا لاجم يف ةفرعملا هيلإ تلصوت ام ثدحا
.ثحبلا رايتخلا ةحضاولا تاريربتلا
: ثحبلا فادهأ
.ثحبلا صخلم يف ةروكذملا ةلكشملاب فادهلأا طبر عم ةقدو حوضوب ثحبلا فادهأ ركذأ
.ةر
: ثحبلا ميمصت
وكذملا فادهلأا زاجنإ ىلع نيثحابلا ةردقم حضوي
عبتتس يتلا تاءارجلإاو قرطلل نيثحابلا كاردإ ىدم حضوي
: لمعلا ةطخ
.حاجنب ثحبلا زاجنلإ ًايلمعو ًايملع ثحاب لك رود
.نيثحابلا لبق نم ثحبلا ةرادإ ةيفيك
.ثحبلا ذيفنتل ينمزلا لودجلا جذومن ةئبعت : ينمزلا لودجلا
: ثحبلا نم ةدافتسلاا
.جئاتنلا نم ةدافتسلاا قرط و ةعقوتملا ثحبلا جئاتن ةيمهأ
: عجارملا
.ثحبلا تايبدأ ضارعتسا يف اهركذل ًاقبط ًلاسلستم ًايددع ًابيترت عجارملا بيترت
ةحفصلا ، ددعلا ، ةلجملا مسأ ، ناونعلا ، نيكراشملا نيثحابلا ، يسيئرلا ثحابلل ةلئاعلا مسأ(
.)ةنسلاو
.)فتاهلاو يلاحلا ناونعلاو دلايملا خيراتو مسلاا( :ةيصخش تامولعم
.اهقبس امو ةيلاحلا ةفيظولا نع تانايبو يلاعلا ميلعتلا لحارم نع تانايب
: ةيتاذلا ةريسلا
.ةروشنملا ثوحبلاو تافلؤملا
ثحبلا ةينازيم
.ثحب عورشم ةينازيم جذومن ةئبعت عم ةيليصفت ةينازيم
.ةيلكلا ةدامع ىدل رتويبمك صرق ىلع ةرفوتم جذامنلا : ةظحلام *
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PROPSAL SUMMARY (English)
1) Title of the Project:
Immunomodulatory and Anticonvulsant profile of some natural products against
Pilocarpine-induced seizures in mice
2) Principal Investigator: Dr. Abdelaziz S. A. Abuelsaad
3) Name of the College: College of Medicine, Taif University
4) Name of the Department: Microbiology & Immunology + Medical Biochemistry
Summary
Pilocarpine-induced seizures can cause pathological changes in many brain regions because of excessive production of free radicals. The objective of this study was to evaluate the immunomodulation; anticonvulsant and antioxidant effects of some natural product extracts in the epilepsy model induced by pilocarpine and investigate two major aims: Firstly; The correlation between the progression of epileptic disease, through studying oxidative stress incidence that could be lead to alteration in proinflammatory molecules, energy levels and neurotransmitter catabolizing enzymes. Secondary; the development of new therapeutic agents of natural extracts with a potent, curative and preventive immunomodulatory and anticonvulsant activities in case of epileptic in murine model. Also; the present study aim to evaluate the efficacy of some natural extracts e.g thymoquinoe; active gradient from Nigella sativa ; Ellagic acid (EA) and Hesperidin (HES) in treating epilepsy comparing it with topiramate, with especial regards to the hypothalamic–pituitary–gonadotropin axis as a biological marker of negative symptoms of epilepsy on fertility, which is the most function exposed to anti-epileptic diseases (AEDs) impairment.
6

INTRODUCTION
Epilepsy is one of the most common neurological conditions that show a prevalence rate in 1–2% of the world population. Even knowing that epilepsy is characterized by a group of brain disorders whose symptoms and causes are diverse they do share a common manifestation: the seizure.
Seizures result from the abnormal discharge of groups of neurons within the brain, usually within a focal point, that can result in the recruitment of large brain regions into epileptiform activity (Fisher, 1989). It has been proved by a number of studies that ion channels are involved in the epileptogenesis and evidences are being obtained that support the behavior of some types of ion channels could be altered during these episodes (Noebels,
2003). Although a considerable number of antiepileptic drugs are available for the treatment of epilepsy there is still an urgent need for development of new drugs as alternatives
(Kupferberg, 1992).
The rich floral biodiversity of has provided herbal health practitioners and other traditional healers in the country with an impressive pool of ‘natural pharmacy’ from which plants are selected as ingredients to prepare herbal remedies and medicines
(phytomedicines) for the treatment, management and control of a variety of human ailments.
Proposal phases:
1.
Epilepsy induction : Sustained seizures were induced by a single intraperitoneal administration of pilocarpine hydrochloride (350 mg/kg b.wt.). During the induction period, mice will subject to follow up behavioral and movement status.
2.
Therapeutic and treatment effects: Murine model will divided into 6 groups as follows: a.
Group (1) : Normal control group: b.
Group (2) : Positive control group induced Epilepsy with pilocarpine as previous.
c.
Group (3) : Normal control group: Epileptic treated with marketing drug; topimerate (EP-TP d.
Group (4) : Normal control group: Epileptic treated with Thymoquinone (EP-
7

TQ) e.
Group (5) : Normal control group: Epileptic treated with Hesperidin (EP-HES) f.
Group (6) : Normal control group: Epileptic treated with Ellagic acid (EP-EA)
3.
Laboratory investigations :
A.
Biochemical estimation of major neurotransmitters e.g: Major neurotransmitters:
 Amino acids: glutamate, aspartate, γ-aminobutyric acid (GABA), glycine

Monoamines and other biogenic amines: dopamine (DA), norepinephrine (noradrenaline; NE, NA), epinephrine (adrenaline), histamine, serotonin (SER, 5-HT)
 Others : acetylcholine (ACh), adenosine, anandamide, nitric oxide, etc.
B.
Blood electrolytes (e.g. Na + , K + ,).
C.
Estimation of oxidative phosphorylation complex I and ATP production rate
D.
Estimation of Inflammatory cytokines e.g (IL-1; IL-6; TNF-a and IFN-g)
E.
Effect on oxidative stress pathways and endogenous antioxidants expression.
F.
Assessment of AChE and ATPase at gene expression, DNA mutation and protein level.
G.
Assessment of genetic mtDNA and nuclear DNA mutations down regulate complex I (NADH dehydrogenase) of oxidative phosphorylation process expression.
4.
Statistical analysis :
Analysis of variance (ANOVA) was used to evaluate differences in mean values among
3 or more groups, and the Dunnett t test was used to test the significance of differences between group pairs. Correlation analysis between 2 variables will be done using the
Pearson parametric method followed by two-way analysis of P value.
8

Epilepsy is a common neurological disorder that consists of a wide variety of symptoms arising from abnormal, excessive or synchronous neuronal activity in the brain
(Engel and Pedley, 1998, Errington et al ., 2005). The occurrence of the disease is slightly higher in males than in females, although most studies showed shifting rates between sexes in different age groups (Forsgren et al., 2 005a&b).
Firstly , Status Epileptics (SE), defined, as recurrent epileptic seizures without complete recovery between seizures, are one of the most serious manifestations of epilepsy.
It is a medical emergency associated with significant mortality and morbidity, which requires immediate and effective treatment (Treiman and Walker, 2006).
Secondly , Temporal Lobe Epilepsy (TLE) is a common neurological disorder common in adults (Engel et al., 1997). Hippocampus, a sub-region of the temporal lobe, is a key brain structure for production of seizers (Clinckers et al., 2004).
Investigations of men who have TLE showed that, 49–71% has diminished potency or sexual interest.
The possible contribution of reproductive endocrine disorders is raised by considerable evidences of gonadal steroid, gonadotropin, and prolactin abnormalities among women and men with epilepsy (Herzog, 2007). Some of these investigations are particularly relevant because they specifically demonstrate associations between hormonal abnormalities and sexual or reproductive dysfunction (Morrell et al ., 2005). Epilepsy is connected to hormones in a complicated manner. It is known that epilepsy itself may affect the hormonal balance and, furthermore, hormones may provoke or inhibit seizures (Herman, 2008).
Quantitative measurement of neurotransmitters, hormones or their metabolites in specific regions in CNS provides important information linking regionally specific chemical events to CNS functions (Gobert et al., 2003). The pulsatile secretion of hypothalamic hormones is controlled by neurotransmitters and by concentrations of hormones, which are secreted in peripheral glands by feedback mechanism (Veldhuis, 2008).
Moreover, fertility is decreased in both men and women with epilepsy (Artama et
9

al., 2004) which may be a consequence of epilepsy itself or the use of antiepileptic medication, which may alter reproductive functions. Today the roles of epilepsy and antiepileptic medication in contributing to reproductive endocrine disorders in people with epilepsy are still widely debated.
The main goal in the treatment of epilepsy is to achieve complete seizure control.
Even though antiepileptic drugs (AEDs) has improved remarkably in terms of efficacy and tolerability during the last decades, still 20-30% of patients with epilepsy suffer from drug resistant epilepsy (Hauser and Hesdorffer, 2001). Once individuals become afflicted with epilepsy, doctors have no way of curing the disease. Rather, current therapies are symptomatic, which means that, the epileptic can take drugs that inhibit seizures, the symptoms of the disease, but these drugs cannot abolish the problem entirely.
Topiramate is one of the new second-generation antiepileptic drugs (AEDs)
(McCabe, 2000). It is a derivative of the naturally occurring monosaccharide D-fructose. It has antiepileptic activity, used as add-on therapy (Fritz et al., 2005), as well as a first line antiepileptic drug (Arroyo et al., 2005) and established efficacy as a migraine preventive
(Diener et al., 2004). TPM was effective in SE suppression in patients (Blumkin et al.,
2005) and in animal models (Fisher et al., 2004). Besides its efficacy with regard to seizures,
TPM can induce cognitive impairments, which are reversible with discontinuation (Mula et al., 2003). In addition, topiramate ingestion produces adverse effects on fertility and reproductive system in adult male rat (Otoom et al., 2004).
Due to the large number of side effects of the AEDs and most of them induce the cytochrome P450 (CYP) enzymatic system, altering the metabolism of sex hormones
(Palmieri and Canger, 2002), there are assessments to use a natural product. In this study we used melatonin (MEL) which is the main product of the pineal gland. Its physiological effects are pleiotropic and it is regarded as “regulator of regulators” (Reiter, 1991). MEL has been involved in the neuroendocrine system, mainly in reproductive physiology (Vázquez et
10

al., 2007). DNA encoding high affinity MEL receptor is used to characterize receptor agonist or antagonists for regulating circadian rhythm disorders or reproductive cycles (Jin et al., 2003). Exogenous MEL is administered in the treatment of circadian rhythms and other functions imbalances It displays a wide spectrum of metabolic and other physiological effects including hypothermic, sedative, hypnotic, analgesic, myorelaxing, cardio- and neuroprotective effects (San and Arranz, 2008; Hlutkin and Zinchuk, 2008; Garzón et al.,
2009; Ambriz-Tututi et al., 2009). Also it has antioxidant properties (Bekyarova, 2009) as well as actions in the immune system (Cardinali et al., 2 008) and in the tumor growth
(Cucina et al., 2009). Metastatic colorectal cancer and breast cancer are treated by melatonin and its analogue is reported (Srinivasan et al.
, 2008).
Role of Mitochondrial metabolic disorders and Epilepsy:
The mitochondrial metabolic disorders affecting the fuel metabolism and energy production was remarkable to mitochondrial dysfunction, other genetic disorders, reduced oxidative phosphorylation (oxidative stress) and insulin resistance (Petersen and Shulman,
2006). This oxidative stress is believed to be a key role event in the pathogenesis of cell death (Jenner and Olanow, 1998). Furthermore, the respiratory chain reaction generates reactive oxygen species (ROS) which causes damage to DNA, proteins, and lipids. As about
95% of molecular oxygen is consumed by the mitochondrial electron transport chain at the cytochrome oxidase complex and the reminder of the oxygen is reduced to hydrogen peroxide and superoxide radicals by the activity of endogenous antioxidant defense system enzymes. Oxygen free radicals are oxygen molecules that carry an extra electron. They are destructive because, in excessive amounts, they chemically attack the components of the cell, including proteins, DNA and lipids in cell membranes. One of the major problems of normal aging is an increased level of these free radical damaged proteins, along with damaged DNA and lipids.
11

Therefore, under dysfunctional and stress conditions, the electron transport system increases the ROS production that will be up taken by mitochondria resulting in mitochondrial dysfunction and oxidative stress which in role are responsible for the pathogenesis of neurons exerting neurodegenerative diseases (Beal MF, 1998). This resulting oxidative stress refers to a state of an imbalance between increased ROS and decreases in endogenous antioxidants within mitochondria enhancing the damaging effects of ROS. The discovery of NADH: ubiquinone oxidoreductase (complex I) dysfunction
(Schapira et al . 1990, Shoffner et al . 1991) led to hypothesis that mtDNA might play a role in the pathogenesis of many of neurodegenerative diseases e.g Alzheimer Disease.
The brain is largely composed of easily oxidized lipids and has a huge number of mitochondria, has a high oxygen consumption rate, and lacks strong antioxidant defenses; it is vulnerable to oxidative injury resulting in neurons death (Jenner and Olanow, 1996).
Specifically, dopaminergic neurons in the substantia nigra (SNc) exhibit hallmarks of oxidative stress, including lipid peroxidation and the enzymatic oxidation of dopamine through its metabolism producing ROS products (Olanow, 1993; Dexter, et al ., 1994).
Moreover, neurodegenerative diseases can affect some forms of memory while leaving others relatively intact (Pennanen et al ., 2006; Ohsawa et al ., 2008).
Activity of Natural Products Extracts Against Neurodegenerative Diseases:
12

Regarding natural products; Medicinal plants serve as sources of direct therapeutic agents and raw materials for the manufacture of more complex compounds, as models for new synthetic products, and as taxonomic markers. Some essential plant-derived drugs are atropine, codeine, morphine, digitoxin/digoxin, and quinine/artemisinin.
Plant-derived drugs have an important place in both traditional and modern medicine.
There is a significant, but far from fully documented, history on the use of fauna and flora by indigenous people for the purpose of preparing medicinal products to treat their ailments.
There are over 3500 species of plants in Sudan, of which more than 90 per cent are native to the country. It is noted that ‘the use of plant remedies by the early the rural areas is not well documented, and most the uses have been communicated by word of mouth…’ leaving the information incomplete and prone to error and mistaken identity.
About 80% of the world's people depend largely on traditional plant-derived drugs for their primary health care (PHC).A number of reviews of the literature indicate that approximately 25 % of the active components of drugs prescribed in 1996 had their origins in higher (flowering) plants, with an additional 10% derived from fungi. A comprehensive review of plant-derived drugs identified a total of 119 drugs, obtained from fewer than 90 plant species (Farnsworth et al ., 1985), including drugs with botanical origins, which are available today, can be divided into a number of categories.
For this reason, a special effort in biological research would undoubtedly help to alleviate human suffering in the long term. Recently, many plants have demonstrated to posses the ability of triggering the apoptotic pathway (Lee, 2007; Ling, 2009; Morais, 2010;
Orlandi, 2010; Romier, 2010 and Schumacher, 2010). The seeds of Nigella sativa Linn
(Ranunculaceae), commonly known as black seed or black cumin, are used in folk/traditional (herbal) medicine all over the world for the treatment and prevention of number of diseases and conditions that include asthma, diarrhoea and dyslipidaemia. The seeds and its oil have antiinflammatory, analgesic, antipyretic, antimicrobial and
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antineoplastic activity. The beneficial effects of the use of the seeds and thymoquinone, the main component of the oil, might be related to their cytoprotective and antioxidant actions, and to their effect on some mediators of inflammation (Ali, 2003).
In addition; hesperidin (HES) is a flavanone glycoside commonly found in the diet in citrus fruits or citrus fruit derived products (Kakadiya et al., 2010; Mahmoud et al., 2012; and Tunon et al., 2009). The anti-inflammatory effects of HES have been characterized in vitro in both rodent and human cell lines (Choi et al., 2003 and Sakata et al., 2003). The scavenging effect of free radicals associated with HES has been evidenced by different neurochemical and neurobehavioral parameters, with HES treatment appearing to reduce expression of proinflammatory mediators like inducible nitric oxide synthase (iNOS), TNF-
α, and IL-1β (Raza et al., 2011 and Youdim et al., 2003). Recently, HES has been shown to exhibit pronounced immunological activities, serving to inhibit inflammatory cell infiltration and mucus hypersecretion in a murine model of asthma (Wei et al., 2012). In addition, HES counteracted the upregulation of proinflammatory cytokines, such as the expression of TNF-
α and IL-1β, in cerebral ischemia (Choi et al., 2007; Rizza et al., 2011 and Yeh et al., 2007), as well as IL-8, IL-6, IL-12, and vascular cell adhesion molecule 1 (VCAM-1), in the case of acute lung inflammation induced by LPS in vivo (Yeh et al., 2007).
The other natural compunds will tested throughout the present proposal will be
Ellagic acid (EA). EA is a dimeric derivative of gallic acid and a phenolic compound often found in many fruits. Interest in EA has increased recently due to its potential antimutagenic and anticarcinogenic effects since it appears to prevent carcingogens binding to protein or
DNA (Heber, 2008). EA is a potent antioxidant, acting as a scavenger of oxygen species produced by hydrogen peroxide treatment, and as a protector of the DNA double helix from alkylating agent injury. Moreover, EA has been recorded as having anti-microbial activity
(Thiem and Goślińska, 2008 and Choi et al., 2011).
It has been demonstrated that EA decreases the TNF-α-induced endothelial
14

expression of VCAM-1 and ICAM-1, which play a critical role in the inflammatory process and in immune response (Papoutsi et al., 2008). EA seems to downregulate the expression of these adhesion molecules without affecting the proliferation rate of cells, with a consequent anti-inflammatory effect. Recently, EA (at 25–50mM) has also been shown to inhibit the IL-
1β-induced endothelial activation and expression of ICAM-1 and VCAM-1 adhesion molecules. It mediates its anti-inflammatory effects via the modulation of NF-kB activity
(Yu et al., 2007) and by inhibiting IL-1β-induced nuclear translocation of p65 and p50, thereby suppressing the expression of VCAM-1 and E-selectin, resulting in decreased monocyte adhesion. Furthermore, Karlsson et al. (2010) conclude that the inhibitory effect of EA acid on the release of prostaglandin E2 (PGE2) from monocytes is due to the suppression of cyclo-oxygenase-2 (COX-2), microsomal PGE synthase-1 (mPGEs-1) and cytosolic phospholipase A2a (cPLA2a), rather than a direct effect on the activities of these enzymes.
Aim of study
Objectives:
The present proposal aims to:
In this work we try to study the efficacy of some natural extracts e.g thymoquinoe; active gradient from Nigella sativa ; Ellagic acid (EA) and Hesperidin (HES) in treating epilepsy comparing it with topiramate, with especial regards to the hypothalamic– pituitary–gonadotropin axis as a biological marker of negative symptoms of epilepsy on fertility, which is the most function exposed to AEDs impairment.
15

) رـــهشلاب ةدملا (
18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1
ثحاب
4
ثحاب
3
ثحاب
2
ثحاب
1
   
ماــــــــهملا
ةيملعلا ةداملا عمج 1
    داوملا و فشاوكلا ءارش 2
   
   
  
تا رضحتسملاو ةيودلأا ءارش 3
ةديدجلا داوملا ريضحت 4
براجتلا تاناويح زيهجت 4
   
  
   
   
   
   
تاناويحلا ىلع براجتلا ءارجإ 5
ايلمعم براجتلا ءارجإ 6
لمعملا يف تانيع لا ليلحت 7
ي ئاصحإ ليلحتو جئاتن عيمجت 8
رشنلل ةيملع ةقرو زيهجت 10
16

Team
Members
PI
1 st
Co-PI
2 nd
Co-PI
3 rd Co-PI
1 Technician
4.3. MANAGEMENT PLAN.
ROLE AND INVOLVEMENT DURATION OF RESEARCH TEAM
Role
Directing and planning

He will be the principal investigator, who will manage the project work plan and coordinate with all project participants.

Animal model construction.

He will arrange for preparation of all reports and manuscripts for publications. He also will guide the research team for the phases and duration of each phase.
 He will prepare the periodical reports for the organization.

He will be responsible for the study of plant extracts and activities using the specific software for the drug development.

He will be responsible for Extracting and fractionation of chemical compound from natural plants and protein analysis.

He also will help in analyzing the data and writing up the scientific papers as well as the periodic scientific reports throughout the project period.

He will arrange for preparation of all reports and manuscripts for publications

He also will help in analyzing the data and writing up the scientific papers as well as the periodic scientific reports throughout the project period.

Animal model construction.

He will carry out all physiological and molecular genetic analysis.

He will help in preparation of the periodical reports for the organization.

He also will help in analyzing the data and writing up the scientific papers as well as the periodic scientific reports throughout the project period.

He will be responsible for the study of plant extracts and activities using the specific software for the drug development.

He will help in preparation of the periodical reports for the organization.

Help in animal model manipulation.

Helping in the blood sampling and arranging the meeting and traveling between the members.
Duration
(months)
18
18
18
18
12
17

The net gain from the project:
The results of this project will be informative to be disseminated in a very well form by: a.
Identifying and quantifying effects of natural product extracts to relief risk factors of Epileptic disease. b.
Sending press releases to journals, magazines, and electronic publications by the end of the year of getting our budget. c.
Participating in local and international events or conferences for Brain diseases and Epilepsy associations. d.
Hosting or attending seminars. e.
Distributing summary documents through the clinical centers and organization for increasing the awareness for the disease and how to diagnose and overcome. f.
Creating and distributing posters throughout the university’s campus. g.
Distributing our discoveries through the pharmaceutical organizations.
VALUE TO THE KINGDOM.
First, the project value will correlated to the results that will help us to give an advice for some of the neurodegenrative diseases. Secondly, through using some natural extracted-compounds used for the first time; the importance of such compounds will be useful for the drug discovery uses in KSA for commercially production after high rates of investigation in wide animal uses
PROJECT OBJECTIVES:
The main objectives in this project are in the following:
Finally the most important indicators which will measure the proposal objective achievement are:

Large scale production of active compound for disease treatment.

Establishment of preclinical studies to investigate nonlethal doses and undesired side effects.

Outstanding and Publishing the mechanism of the disease.
18

1) Ali BH. Pharmacological and toxicological properties of Nigella sativa. 2003.
2) Ambriz-Tututi, M.; Roha-González, H.; Cruz, S. L. and Granados-Soto, V. (2009): Melatonin: A hormone that modulates pain. Life Sci., 84 (15-16): 489-498.
3) Arroyo, S.; Dodson, W.; Privitera, M..; Glauser, T.; Naritoku, D.; Dlugos, D.; Wang, S.; Schwabe, S. and Twyman, R. (2005): Randomized dose-controlled study of topiramate as first-line therapy in epilepsy. Acta Neurol. Scand., 112: 214-222.
4) Artama, M.; Isojärvi, J.; Raitanen, J. and Auvinen, A. (2004): Birth rate among patients with epilepsy: a nationwide population-based cohort study in Finland. Am. J. Epidemiol., 159: 1057-
1063.
5) Bekyarova, G.; Tancheva, S. and Hristova, M. (2009): Protective effect of melatonin against oxidative hepatic injury after experimental thermal trauma. Methods Find Exp. Clin. Pharmacol.,
31 (1): 11-14.
6) Blumkin, L.; Lerman-Sagie, T.; Houri, T.; Gilad, E.; Nissenkorn, A.; Ginsberg, M. and Watemberg,
N. (2005): Pediatric refractory partial status epilepticus responsive to topiramate. J. Child. Neurol.,
20: 239-241.
7) Cardinali, D.; Esquifino, A.; Srinivasan, V. and Pandi-Perumal, S. (2008): Melatonin and the
Immune System in Aging. Neuroimmunomodulation, 15: 272-278.
8) Carlson, H.; Ronne-Engstrom, E.; Ungerstedt, U. and Hillered, L. (1992): Seizure related elevations of extracellular amino acids in human focal epilepsy. Neurosci. Lett., 140: 30-32.
9) Choi I. Y., S. J. Kim, H. J. Jeong et al., “Hesperidin inhibits expression of hypoxia inducible factor-1 alpha and inflammatory cytokine production from mast cells,” Mol Cell Biochem, vol. 305, no. 1-2, pp. 153-61, Nov, 2007.
10) Choi JG, Kang OH, Lee YS, Chae HS, Oh YC, Brice OO et al. (2011) In Vitro and In Vivo
Antibacterial Activity of Punica granatum Peel Ethanol Extract against Salmonella. Evid Based
Complement Alternat Med; 1-8.
11) Choi Y.-J., J.-S. Kang, J. H. Y. Park et al., “Polyphenolic flavonoids differ in their antiapoptotic efficacy in hydrogen peroxide–treated human vascular endothelial cells,” J.Nutr., vol. 133, no. 4, pp. 985-991, 2003.
12) Clinckers, R.; Smolders, I.; Meurs, A.; Ebinger, G. and Michotte Y. (2004): Anticonvulsant action of hippocampal dopamine and serotonin is independently mediated by DA and 5-HT receptors. J.
Neurochem., 89: 834-843.
13) Cucina, A.; Proietti, S.; D'Anselmi, F.; Coluccia, P.; Dinicola, S.; Frati, L. and Bizzarri, M. (2009):
Evidence for a biphasic apoptotic pathway induced by melatonin in MCF-7 breast cancer cells. J.
Pineal. Res., 46 (2): 172-180.
14) Diener, H.; Tfelt-Hansen, P.; Dahlof, C.; Lainez, M.; Sandrini, G.; Wang, S.; Neto, W.;
Vijapurkar, U.; Doyle, A. and Jacobs, D. (2004): Topiramate in migraine prophylaxis - results from
19

a placebo-controlled trial with propranolol as an active control. J. Neurol., 251: 943-950.
15) Engel, J. and Pedley, T. (1998): Introduction: what is epilepsy? In: Engel, J. r. and Pedley, T. A.
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16) Engel, J.; Williamson, P. and Wieser, H. (1997): Mesial temporal lobe epilepsy. In: Engel, J. r. and
Pedley, T. A. (eds). Epilepsya: A comprehensive textbook, Vol. 1. Lippincott-Raven Publishers,
Philadelphia, pp: 2417-2426.
17) Errington, A.; Stöhr, A. and Lees, G. (2005): Voltage Gated ion Channels: Targets for
Anticonvulsant Drugs. Current Topics in Medicinal Chemistry, (5): 15-30.
18) Fisher R.S, Animal models of the epilepsies. Brain Res. Rev.1989; 14: 245–278.
19) Fisher, A.; Wang, X.; Cock, H.; Thom, M.; Patsalos, P. and Walker, M. (2004): Synergism between topiramate and budipine in refractory status epilepticus in the rat. Epilepsia, 45: 1300-1307.
20) Forsgren, L.; Beghi, E. and Ekmanc, M. (2005b): Cost of epilepsy in Europe. Eur. J. Neurol., 12
(1): 54-58.
21)
Forsgren, L.; Beghi, E.; O˜ un A. and Sillanpaa, M. (2005a): The epidemiology of epilepsy in
Europe a systematic review. Eur. J. Neurol., 12: 245-253.
22) Fritz, N.; Glogau, S.; Hoffmann, J.; Rademacher, M.; Elger, C. and Helmstaedter, C. (2005):
Efficacy and cognitive side effects of tiagabine and topiramate in patients with epilepsy. Epilepsy
Behav., 6: 373-381.
23) Frungieri, M.; Gonzalez-Galvar, S. and Calandra, R. (1996): Influence of photoinhibition of GABA and glutamic acid levels, and on glutamate decarboxylase activity in the testis and epididymis of the golden hamster. Int. J. Andral., 19 (3): 171-178.
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Garzón, C.; Guerrero, J. M.; Aramburu, O. and Guzmán, T. (2009): Effect of melatonin administration on sleep, behavioral disorders and hypnotic drug discontinuation in the elderly: A randomized, double-blind, placebo-controlled study. Aging Clin. Exp. Res., 21 (1): 38-42.
25) Gobert, A.; Di Cara, B.; Cistarelli, L. and Millan, M. (2003): Piribedil enhances frontocortical and hippocampal release of acetylcholine in freely moving rats by blockade of alpha 2A-adrenoceptors:
A dialysis comparison to talipexole and quinelorane in the absence of acetylcholinesterase inhibitors. J. Pharmacol. Exp. Ther., 305: 338-346.
26) Hauser, A. and Hesdorffer, D. (2001): Epidemiology of intractable epilepsy. In: H. Luders and Y.
Comair (eds.) Epilepsy surgery. Philadelphia: Lippincott, Williams and Wilkins, pp: 55-61.
27) Heber D. (2008) Multitargeted therapy of cancer by ellagitannins. Cancer Lett; 269: 262-8.
28) Herman, S. (2008): Sex Hormones and Epilepsy: No longer Just for women. Epilepsy Curr., 8 (1):
6-8.
29) Herzog, A. (2007): Reproductive endocrine considerations and hormonal therapy for men with epilepsy. Epilepsia, 32 (S6): S34-S37.
30) Hlutkin, S. and Zinchuk, V. (2008): Effect of melatonin on the blood oxygen transport during hypothermia and rewarming in rats. Adv. Med. Sci., 53 (2): 234-239.
20

31) Isaac, M. (2005): Serotonergic 5-HT2C Receptors as a potential therapeutic target for the design antiepileptic drugs. Current Topics in Medicinal Chemistry, 5: 59-67.
32) Jin, X.; von Gall, C.; Pieschl,R.; Gribkoff,V.; Stehle,J.; Reppert,S. and Weaver, D. (2003):
Targeted disruption of the mouse Mel1b melatonin receptor. Mol. Cell Biol., 23 (3): 1054-1060.
33)
Kakadiya J., M. Shah, and N. J. Shah, “Effect of nebivolol on serum diabetic marker and lipid profile in normal and streptozotocin-nicotinamide induced diabetic rats.,” RJPBCS 1 vol. 1, pp.
329-334. ,2010.
34) Kanner, A. and Balabanov, A. (2002): Depression and epilepsy, How closely related are they?
Neurol., 58: S27-S39.
35) Karle, J.; Laudrup, P.; Sams-Dodd, F.; Mikkelsen, J. and Nielsen, M. (1997): Differential changes in induced seizures after hippocampal treatment of rats with an antisense oligodeoxynucleotide to the GABA(A) receptor gamma2 subunit. Eur. J. Pharmacol., 340 (2-3): 153-160.
36)
Karlsson S, Nånberg E, Fjaeraa C, Wijkander J. (2010) Ellagic acid inhibits lipopolysaccharideinduced expression of enzymes involved in the synthesis of prostaglandin E2 in human monocytes.
Br J Nutr; 103: 1102-9.
37) Kupferberg H.L, Strategies for identifying and developing new anticonvulsant drugs, Pharm.
Weekblad 1992; 14:132–138.
38) Lee SH. Inhibitory effect of eupatilin and jaceosidin isolated from Artemisia princeps in IgEinduced hypersensitivity. 2007.
39) Liao, C.; Zheng, J.; David, L. and Nicholson, R. (2004): Inhibition of voltage-sensitive sodium channels by the cannabinoid 1 receptor antagonist AM 251 in mammalian brain. Basic. Clin.
Pharmacol. Toxicol., 94 (2): 73-78.
40) Ling Y. Endostar induces apoptotic effects in HUVECs through activation of caspase-3 and decrease of Bcl-2. 2009.
41) Mahmoud A. M., M. B. Ashour, A. Abdel-Moneim et al., “Hesperidin and naringin attenuate hyperglycemia-mediated oxidative stress and proinflammatory cytokine production in high fat fed/streptozotocin-induced type 2 diabetic rats,” J Diabetes Complications, vol. 26, no. 6, pp. 483-
90, Nov-Dec, 2012.
42) McCabe, P. (2000): New anti-Epileptic drugs for the 21st century. Expert Opinion. Pharmacother.,
1: 633-674.
43) Morais C. Anti-angiogenic actions of pyrrolidine dithiocarbamate, a nuclear factor kappa B inhibitor. 2009.
44) Morrell, M.; Flynn, K.; Done, S.; Flaster, E.; Kalayjian, L. and Pack, A. (2005): Sexual dysfunction, sex steroid hormone abnormalities, and depression in women with epilepsy treated with antiepileptic drugs. Epilepsy Behav., (6): 360-365.
45) Mula, M.; Trimble, M. and Sander, J. (2003): The role of hippocampal sclerosis in topiramaterelated depression and cognitive deficits in people with epilepsy. Epilepsia, 44: 1573-1577.
46) O'Hearn, E. and Molliver, M. (2004): Administration of a non-NMDA antagonist, GYKI 52466, increases excitotoxic Purkinje cell degeneration caused by ibogaine. Neurosci., 127 (2): 373-383.
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47) Orlandi A. Flt-1 expression influences apoptotic susceptibility of vascular smooth muscle cells through the NF-kappaB/IAP-1 pathway. 2010.
48) Otoom, S.; Batieneh, H.; Hassan, Z. and Daoud, A. (2004): Effects of long-term use Topiramate on fertility and growth parameter in adult male rats. Neuroendocrinol. Lett., 25 (5): 351-355.
49) Palmieri, C. and Canger, R. (2002): Teratogenic potential of the newer antiepileptic drugs: what is known and how should this influence prescribing? CNS Drugs., 16 (11): 755-764.
50) Papoutsi Z, Kassi E, Chinou I, Halabalaki M, Skaltsounis LA, Moutsatsou P. (2008) Walnut extract
[Juglans regia L.] and its component ellagic acid exhibit anti-inflammatory activity in human aorta endothelial cells and osteoblastic activity in the cell line KS483. British Journal of Nutrition; 99:
715-22.
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2011.
52) Reiter, R. (1991): Melatonin: The chemical expression of darkness. Mol. Cell Endocrinol., 79:
C153-C158.
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Rizza S., R. Muniyappa, M. Iantorno et al., “Citrus polyphenol hesperidin stimulates production of nitric oxide in endothelial cells while improving endothelial function and reducing inflammatory markers in patients with metabolic syndrome,” J Clin Endocrinol Metab, vol. 96, no. 5, pp. E782-
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55) Sakata K., Y. Hirose, Z. Qiao et al., “Inhibition of inducible isoforms of cyclooxygenase and nitric oxide synthase by flavonoid hesperidin in mouse macrophage cell line,” Cancer letters, vol. 199, no. 2, pp. 139-145, 2003.
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57) Schumacher M. Heteronemin, a spongean sesterterpene, inhibits TNFalpha-induced NF-kappaB activation through proteasome inhibition and induces apoptotic cell death. 2010.
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Youdim KA, Dobbie MS, Kuhnle G et al., “Interaction between flavonoids and the blood-brain barrier: in vitro studies,” J Neurochem. , vol. 85, no. (1), pp. 180-92, 2003.
70) Yu YM, Wang ZH, Liu CH, Chen CS. (2007) Ellagic acid inhibits IL-1b-induced cell adhesion molecule expression in human umbilical vein endothelial cells. Br J Nutr 2007; 97: 692-8.
23

12000
10000
10000
12000
10000
10000
10000
42000
------------
------------
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10000
12 months
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4000
100000
114000
146000
18 months
ريغلا طقف ىدوعس ريال 146000
24

Units/
Item kits
1.
Superoxide dismutase (SOD) kits
2.
Glutathione peroxidase (GPx) kits
3.
Glutathione reductase (GR) kits
4.
Glutathione-S-transferase (GST) kits
5.
Reduced glutathione (GSH) kits
6.
Malondialdehyde (MDA) kits
7.
Nitric oxide (NO) kits
8.
ATP Determination Kit
9.
Mitochondrial H
2
O
2
Kit
10.
RNA Extraction kit
11.
DNA Extraction kit
12.
cDNA kit for GAPDH gene
13.
RT-PCR kit for inflammatory cytokines
14.
Western blotting kits for mitochondrial proteins
15.
ELISA kits for murine IL-1
16.
ELISA kits for murine IL-10
17.
ELISA kits for murine TNF-a
18.
ELISA kits for murine IFN-g
19.
ELISA kits for murine COX
20.
ELISA kits for murine IL-13
Unit price/
SR
2
2
2
2
2
2
2
2
2
4
4
4
4
4
1
1
1
3500
3500
3500
1
1
3500
3500
1 3500
Total Price
500
500
500
500
500
500
500
4000
4000
3500
2000
2000
2500
4000
Total/
SR
1000
1000
1000
1000
1000
1000
1000
8000
8000
14000
8000
8000
10000
16000
3500
3500
3500
3500
3500
3500
100,000 SR
25

1)
:

Name : Abdelaziz SA Abuelsaad

Date of Birth : 8-11-1967

Permanent Position: Ass. Prof. Faculty of Science, Beni-Suef University, Egypt.

Current Position : Associated Professor, College of Medicine, Taif University.

E-Mail : elsaad1@yahoo.com OR elsaad22@tu.edu.sa OR elsaadtaif@gmail.com

Tel: +966555671460

General Specialty : Immunology

Fine Specialty : Immunomodulation activity against Aeromonas hydrophila .
2)
No.
1
Degree University/Institution
B. Sc. Faculty of Science, Cairo University (Beni-Suef Branch)
2
3
M. Sc Faculty of Science, Minia University, Minia, Egypt.
Ph. D Faculty of Science, Cairo University (Beni-Suef Branch)
Year
1989
1996
2001
3)
Jan.2009 to date: Associate Professor of Medical Biology, College of Medicine and
Medical Sciences, Taif University, KSA.
Sep. 2005 to Jan. 2009: Assistant Professor of Medical Biology, College of Medicine and Medical Sciences, Taif University, KSA.
Nov. 2001 to Sep. 2005: Lecturer (Assistant Professor) of Immunology, Zoology
Department, Faculty of Science at Beni-Suef branch, Cairo University.
Nov. 1996 to Oct. 2001: Assistant Professor of Zoology, Zoology Department,
Faculty of Science at Beni-Suef branch, Cairo University.
26

Jan. 1990 to Nov. 1996: Demonstrator of Zoology, Zoology Department, Faculty of
Science at Beni-Suef branch, Cairo University.
4) TEACHING RESPONSIBILITIES
1Basic and Advanced Immunology course for 4th year students, Faculty of Science,
Beni Suef University, Egypt from 2001 to 2005.
2Immunology Course for 3rd year students, Faculty of Education, Beni Suef
University, Egypt from 2001 to 2005.
3Practical Immunology Course for 4th year students, Faculty of Science, Beni Suef
University, Egypt from 1990 to 2005.
4Practical Physiology Course for 4th year students, Faculty of Science, Beni Suef
University, Egypt from 1990 to 2001.
5Practical Ecology and Animal Fauna Course for 3rd year students, Faculty of
Science, Beni Suef University, Egypt from 1990 to 2001.
6Shared in Teaching of Parasitology course for 3rd year students, Faculty of Science,
Beni Suef University, Egypt from 2001 to 2005.
7Practical Parasitology course for 3rd year students, Faculty of Science, Beni Suef
University, Egypt; from 1990 to 2001.
8Shared in Teaching of Immunology for 2nd year MBBS students, College of
Medicine and Medical Sciences, Taif University, Saudi Arabia, 2006-till now.
9Shared in Teaching of Medical and Pharmaceutical Biology for 1st year Pharmacy students, Taif University, Saudi Arabia, from 2005-2010.
10Shared in Teaching of Medical Biology for 1st year Medical Sciences students, Taif
University, Saudi Arabia, from 2005-2010.
11Teaching of Basic Physiology for preparatory year students, Medical division
(Health Pathway), Taif University, Saudi Arabia from 2012-till now.
12Teaching of Basic Cytology and Histology for preparatory year students, Medical division (Health Pathway), Taif University, Saudi Arabia from 2012-till now.
5)
AREAS:
1Immunobiology,
2Immunoparasitology,
3Development of new diagnostic tools for different parasitic infections.
4Virology research activities.
27

6)
A- Innate immune response:


Separation and purification of monocytes and polymorphonuclear leukocytes.
Peritoneal Macrophage preparation and culture.


Estimation of the phagocytosis index ( In Vitro ).
Determination of the chemokinetic and chemotactic activity of the leukocytes.
B- Humoral immune response
 Quantitative assay for the total immunoglobulins.
 Determination of the precipitins by double immunodiffusion assay.


Enzyme Linked Immunosorbent Assay
Western blot
 Haemagglutination inhibition
 Passive haemagglutination
 Agar gel precipitation test.
C- Cell-mediated immune response:
 Determination of different cytokines by ELISA.


Cell separation, culturing and proliferation.
Lymphocyte blastogenesis by MTT dye uptake method
 Oxidative burst evaluation (Superoxide dismutase, catalase, nitrogen oxide,
Lipid peroxidation).
 Hormone binding assay Interleukin assays by ELISA.
D- Other experiences:
 Experimental Parasitic infection.


Worm recovery from infected animals.
Parasite culture in vitro .


Medical Snails housing and breading.
 Dealing with different experimental animals (rats, mice).
 Isolation of blood cells and tissues
Biochemical assays for liver and renal functions
28

E- Molecular diagnostic techniques
 Protein purification
 DNA and RNA manipulation
 PCR and RT PCR as well as real time PCR
7)
1Abdel-Moneim, A.; Sakran, Th. F.; El-Feki, M.A. and Abdelaiz SA. Abuelsaad
Biochemical and histochemical changes of common carp ( Cyprinus carpio L.) against the infection with Aeromonas hydrophila
”. Published in Journal Union of
Arab Biologists, November 1997, 8 (A).
2Sakran, Th. F.; Abdel-Moneim, A.; El-Feki, M.A. and Abdelaiz SA. Abuelsaad
“Histopathological changes of common carp (
Cyprinus carpio L.) against the infection with Aeromonas hydrophila
”. Published in Journal Union of Arab
Biologists, November 1997, 8 (A) Zoology, 279-303: With others.
3El-Feki, M.A.; Abdel-Moneim, A.; Sakran, Th. F.; and Abdelaiz SA. Abuelsaad
(1996): Immunological changes of common carp ( Cyprinus carpio L.) against the infection with Aeromonas hydrophila
”. Published in Journal of Veterinary
Medicine Conference, Assuit University, Assuit, Egypt November, 1996.
4El-Feki, M.A.; Abdel-Moneim, A.; Sakran, Th. F.; and Abdelaiz SA. Abuelsaad
(2001): Immunological studies on albino rats against crude endotoxins of
Aeromonas hydrophila
” at First International Conference for Egyptian British
Biological society (EBB, Soc), Suez Canal University, Ismalia, Egypt, Vol.(1),
29th –30th July, 2001.
5Awad, E. M. and Abdelaiz SA. Abuelsaad (2004): Orchestral effects of some growth factors on endothelial cell proliferation. J. Egy.Germ. Soc. (41 A).
235:253 www.egsz.com
; ISSN: 1110-50364
6Abdelaiz SA. Abuelsaad and Abdel-Moneim, A. S . (2005): Modulation of macrophage functions by sheep poxvirus provides clues to understand interaction of the virus with host immune system. Virology Journal, 2:22. http:// www.virologyj.com/content/2/1/22 .
7Abdelaiz SA. Abuelsaad and Mahmoud, H. M. (2005): Prevalence of
AflatoxinB1- Reproductive and Oxidative Toxicity and the Improvement of
Phytic acid administration in Male Albino Rats. J. Egy.Germ. Soc. (48 A) 2005:
333-353.
8Abdella, E.M.; Abo El-Hadid, S.M.; Allam, G.; Abdelaiz SA. Abuelsaad.
Random amplified polymorphic DNA-based analysis reveals genetic variations in the
29

populations of the tapeworm Hymenolepis species isolated from rodents. J.
Egy.Germ. Soc., (51 D): 69-89. www.egsz.com; ISSN: 1110-50364 .
9Abdelaiz SA. Abuelsaad (2006): An immunological Study Challenge with
Lipopolysaccharides against Murine Schistosomiasis Infection. Egypt J. Zool.
47: 319-336.
10Abdelaiz SA. Abuelsaad and Ebaid, H.
(2006): Genetic Variations in Maternal
Transfer and Immune Responsiveness for Some Indigenous Chicken Lines to
Infectious Bronchitis and Reoviruses. Egypt J. Zool. 47: 203-220 www.egsz.com; ISSN: 1110-50364.
11Abdelaiz SA. Abuelsaad (2007): Immunomodulating Effect of inositol hexaphosphate against LPS of Aeromonas hydrophila -endotoxin.
Immunobiology, 212 (3) 179:192.
12Kandeil, M. A and Abdelaiz SA. Abuelsaad (2005): Biochemical effects of
Ascorbic acid on AflatoxinB1-Induced Oxidative Stress in Male Albino Rats).
The 4 th International Scientific Conference, Faculty of Veterinary Medicine of
Teramo University, Italy &, Mansoura University at Mansoura, Egypt, 5-6 April,
2005.
13Allam, Gamal AA and Abdelaiz SA. Abuelsaad (2008): Potential effects of
Astragalus membranaceus polysaccharides on adult schistosomes and their egg production in vitro and in vivo. Egypt. J. Zool (51). 425:447.
14Mahmoud, HM; Abdelaiz SA. Abuelsaad ; Th F. Sakran and Hafez, SM. (2009):
Lake Qaroun contamination with heavy metals and its impact on parasitic infection of Mugil cephalus.
11th Int. Conf. Enviro. Sci. Tech., Greece. 3-
9/9/2009.
15Ahmed S. Abdel-Moneim; Gaber M. G. Shehab; and Abdelaiz SA. Abuelsaad
(2011): Molecular evolution of the six internal genes of H5N1 equine influenza.
Arch Virol 2011.
16Gamal G. Badr; Hossam Ebaid; Mohamed Mohany and Abdelaiz SA. Abuelsaad .
(2012): Modulation of immune cell proliferation and chemotaxis towards CC chemokine ligand (CCL)-21 and CXC chemokine ligand (CXCL)-12 in undenatured whey protein-treated mice. J Nutr Biochem. 2012 Dec;
23(12):1640-6.
17Abdel-Moneim, AS; Bamaga, MS, Abdelaiz SA. Abuelsaad , Shehab,
G.M., Farahat, F.M. (2012). HCV infection among Saudi population: High prevalence of genotype 4 and increased. PLoS ONE| www.plosone.org.
18Ahmad F. Eweas, Gamal Allam, Abdelaiz SA. Abuelsaad . Abuelsaad, Ibrahim A.
Maghrabi, and Abdul Hamid AlGhamdi. Design, synthesis, anti-schistosomal
30

activity and molecular docking of novel 8- Hydroxyquinoline -5-sufonyl 1,4diazepine derivatives. Bioorganic Chemistry (2013).
19H. S. Hafez, DA. Ghareeb, HM Hussien, HEA. Ahmed, Abdelaiz SA. Abuelsaad .
(2013): Insulin Resistance Exacerbates Impairments in Cholinergic System
Leading to Alzheimer´s Disease: With Emphasis of Ipriflavone Supplementation as Drug Therapy. 11 th
International Conference in PD/AD in Florence-Italy. 6-11
March 2013.
20Abdelaiz SA. Abuelsaad , Abdel-Hamid S. Al-Ghamdi, Ahmed N. Al-Ghamdi,
Eyad A Alakkas, Adnan A M. A. Alsulaimani, Abdulla A. Al-Harthi and Ahmed
S. Abdel-Moneim. Allocation of new mutant sites of HIV-1 coreceptor CCR5 among Saudi populations. Journal of Interferon
& Cytokine Research (2013).
21Ahmad F. Eweas, Gamal Allam, Abdelaiz SA. Abuelsaad , Ibrahim A. Maghrabi, and Abdul Hamid ALGhamdi (2013). Synthesis, Anti-Schistosomal Activity and
Molecular Modeling of Two Novel 8- Hydroxyquinoline Derivatives. Anti-
Infective Agents 11(1).
22Allam, Gamal AA and Abdelaiz SA. Abuelsaad (2013): Differential effect of hesperidin on Th1, Th2, Th17, and proinflammatory cytokines production from splenocyte of Schistosoma mansoni -infected mice. Central European Journal of
Immunology 38 (1): 29-36).
23Allam, Gamal AA and Abdelaiz SA. Abuelsaad (2013): In vitro and in vivo effects of hesperidin treatment on adult worms of Schistosoma mansoni . Journal of Helminthology, May 9:1-9.
24Allam, Gamal AA; Ahmad F. Eweas and Abdelaiz SA. Abuelsaad (2013): In vivo schistosomicidal activity of three novels 8-hydroxyquinoline derivatives against adult and immature worms of Schistosoma mansoni . Parasitology Research; online at 22th June-2013.
25Abdelaiz SA. Abuelsaad ; Imad Mohamed; Gamal Allam and Adnan Al-Solumani.
(2013): Antimicrobial and immunomodulating activities of hesperidin and ellagic acid against diarrheic Aeromonas hydrophila in murine model. Life Sciences,
93:714-722
26Abdelaziz SA Abuelsaad (2014): Supplementation with Astragalus polysaccharides alters Aeromonas -induced tissue-specific cellular immune response . Microbial
Pathogenesis: 66 (2014) 48-56.
27Abdelaiz SA. Abuelsaad ; Gamal Allam and Adnan Al-Solumani. (2014):
Hesperidin inhibits inflammatory response induced by Aeromonas hydrophila infection and alters CD4+/CD8+ T cell ratio. Accepted In Press Journal "Mediators of
Inflammation".
31

28Adnan A Alsulaimani and Abdelaziz SA Abuelsaad (2014): Early inflammatory biomarkers of neonatal hypoxic encephalopathy. Accepted In Press "Journal of Pediatric
Sciences.
8)
1.
Poster presentation at 9 th
International Conference of Autoimmunity in Nice-Paris 26-30
March 2014.
2.
Poster presentation at 11 th
International Conference in PD/AD in Florence-Italy 6-11
March 2013.
3.
Attendance of a two days Course: Promotion of Scientific Research Skills for Academic
Staff in Saudi Universities. Organized by Taif University, 14 – 15 March 2009.
4.
Attendance of a five days training program: Integrated Program on Preparation of Trainers.
Organized by Institute of Research and Consulting Services, Imam Mohamed bin Saud
Islamic University in collaboration with Taif University, KSA, 28 March - 1 April 2009.
5.
Attendance of a three days training program: Strategic Planning in Academic institutions.
Organized by Institute of Research and Consulting Services, Imam Mohamed bin Saud
Islamic University in collaboration with Taif University, KSA, 4-6 April 2009.
6.
Certificate of attendance in Formulating a Research Proposal Symposium. 30 th
Nov-1st
Dec. 2010 hold at College of medicine, Taif University, Taif, KSA.
7.
Certificate of attendance the 1st. Taif Forum for Enhancement of Medical Education, 27th
-29th May 2007,Taif Univ. Taif, KSA
8.
Certificate of attendance the development of researching skills of faculty members, 14-15
March 2009, Taif University.
9.
1st Joint Meeting of European National Societies of Immunology; under the auspices of
EFIS; 16th European Congress of Immunology - ECI; September 6-9, 2006 - Paris, France
(Poster Presentation).
10.
The Fourth International Scientific Conference, Faculty of Veterinary Medicine of
Teramo University, Italy- Mansoura University at Mansoura, Egypt, 5-6 April, 2005.
11.
14 th
International Conference of the Egyptian-German Society of Zoology (Fayoum-
Sharm-elShiekh) Faculty of Science, Fayoum, Cairo University, Egypt. (28th Feb.-4th
March 2004).
32

12.
13 th
International Conference of the Egyptian-German Society of Zoology (Beni-Suef &
Ghurgada) Faculty of Science, Beni-Suef, Cairo University, Egypt. (2nd -5th March
2003).
13.
First International Conference for Egyptian British Biological Society (EBB Soc.), Suez
Canal University, Ismalia Egypt., 29th – 30th July, 2001.
14.
Conference of Union of Arab Biologists at Beni-Suef & Fayoum, November 1997.
15.
Conference of Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt
November 1996.
9)
1.
Egyptian Society of immunologist.
2.
Egyptian-German Society for Zoology.
3.
Egyptian Society of Zoology.
4.
Arab Biologists Union Society.
5.
Egyptian Society of Experimental Biology
6.
Saudi Biological Society
10)
1.
Design, Synthesis and Anti-Schistosomal activity of Novel 8-hydroxyquinolne derivatives. Co-investigator , Grant # 1-432-1222 (2010-2011, completed), funded by Taif University.
2.
Evaluation of Potential Effect of Some Natural Immunomodulators on treatment of Schistosomiasis and their Pathological Consequences. Coinvestigator , Grant # 2-433-1571 (2011-2013, completed), funded by Taif
University.
3.
Delineating the inflammatory cascades and pathways during invasion of
Aeromonas hydrophila to human intestinal cells causing diarrhea in murine model. Principle-investigator , Grant#1-432-1243 (2010-2012, completed), funded by Taif University.
4.
Prophylactic and Therapeutic Effect of Ellagic Acid on Collagen-Induced
Arthritis. Co-investigator , Grant# 1-435-3215 (2014-till now, running), funded by Taif University.
33

5.
Studies on the Protective Potential Effects of Some Natural Products on
Aeromonas hydrophila -Induced Sepsis upon Endothelial Cell Line. (In Vivo and In Vitro Studies). Academic year 2006-2008. ( Principal investigator ).
6.
Biological and Molecular Studies on the susceptible and non-susceptible snail infection with Schistosoma sp. in West-Region of KSA). Academic year 2007-
2008 (Co-investigator).
7.
Delineating the immunological basis for increased susceptibility to vaginal candidosis in diabetic females: The estrogen connection. Academic year 2008-
2009. ( Co-investigator ).
8.
Human Viral Diseases Map in Taif, a joint project with Al-Hada Hospital
{Approved by Al-Hada Committee] May 2010- ( Co-investigator ). Preparation of H5N1 DNA vaccine [submitted project to Taif University: Decision is in progress]. Academic year 2009-2010 (Co-investigator).
9.
Genotyping and Subtyping of Hepatitis C virus isolates from Saudi Arabia.
Funded by Taif University, Grant no. 1-1432-1189 ( Co-investigator ).
Academic year 2010-2011
10.
Identification of the CCR5-Δ 32 HIV resistance allele in Saudi citizens. Funded by Taif University, ( Co-investigator ). Academic year 2010-2011
34

Personal Data
Family name: Shehab First name: Gaber Middle names: Mohamed Gomaa
Permanent Address Villa 6, in front of Alrabouh, Al-Shaikh Zayed City, 6 October, Egypt.
Work Address
Department of Biochemistry, Faculty of Agriculture, Cairo University,
Giza, Egypt. PO Box 12613
(+966) 27323820 (home)
Telephone Fax (+966) 27250528
Place of birth
(+966) 27242295 (work)
0595410730 & 0550561429 (mobile)
Cairo
Date of birth 16-04-1969 (D-M-Y)
Marital status
Nationality
Married
Egyptian
Academic qualifications:
Number of children
Email
3 (Moath 1/11/1999 ; Yousef
23/08/2002 & Maryam 17/3/2007) gshehab@tu.edu.sa g.shehab@hotmail.com
Degree Department College/Faculty University Major Date
B.Sc.
M.Sc.*
Biochemistry
Biochemistry
Faculty of
Agriculture
Faculty of
Agriculture
Cairo University,
Egypt
Cairo University,
Egypt
Biochemistry
Biochemistry
June, 1991
August,
1996
Ph.D.** Biological and
Biomedical
Sciences
Durham
University, UK
Molecular biology and
Biochemistry
August,
2002
Assistant
Professor
Professsor
Biochemistry
Biochemistry
Faculty of
Agriculture
Faculty of
Agriculture
Cairo University,
Egypt
Cairo University,
Egypt
Biochemistry
Biochemistry
2007
2014
*Under the title of "Biochemical Studies on Some Plant Lectins"
**Under the title of "Molecular aspects of disease resistance in potato Solunum tuberosum L."
35

Career history:
University From To Job title Inclusive duties Type of job
Cairo University,
Egypt
Cairo University,
Egypt
Durham University,
UK
Cairo
Egypt
University,
Taif University,
Saudi Arabia
University,
1991 1996 demonstrator Teaching practical courses& researcher
1996 1998 Assistant lecturer Teaching practical courses & research
1998 2002 Researcher (Ph.D.
Student) research
2002 2005 lecturer
2005 now Associate
Professor
Teaching theoretical courses & research
Teaching theoretical and practical courses
3/2014 now Professor
& research
Full time
Full time
Full time
Full time
Full time
Cairo
Egypt
Experience
1- Protein isolation & purification
3- Isoenzyme separation and detection
5- Genomic DNA isolation
7- Constructing & screening cDNA libraries
2- Western blotting
4- 1&2 dimensional gel electrophoresis
6- Total RNA & mRNA purification
8- Northern and Southern blotting
9- PCR technology
11- Sequencing & sequence analysis
10- Preparing probes
12- Bioinformatics
13- DNA microarray
15- General chromatographic and chemical analysis
14- Teaching
Teaching
Practical and theoretical courses of:
1- Biochemistry 2- Molecular biology
4- Protein chemistry
7- Organic chemistry
5- Analytical chemistry
8- Inorganic chemistry
10- Cell and Genetics 11- General Chemistry
3- Biotechnology
6- Nucleic acids
9- Medical Biochemistry
12- Clinical Chemistry
36

Activities:
A) Conferences, Symposiums & Workshops
1.
Attended the “Sixth International Symposium on nitrogen fixation with non-legumes” at Ismailia, Egypt; (6 -10 th of September, 1993).
2.
Presented a poster under the title of “Radical Responses to stress in potatoes”; at The
13 th John Innes Symposium; ‘Attack & Defence in Plant Disease’; Norwich, UK, 20-
23rd July 1999.
3.
Doherty, S. Johnson, S.M., Shehab, G.M.G. & Croy, R.R.D
4.
Presented a poster under the title of "The molecular bases of potato durable resistance"; at the University of Durham, UK (May, 2002).
5.
Shehab, G.M.G. & Croy, R.R.D
6.
Attended a workshop on “Distance Learning in Agriculture Education and Training:
The Concept, Potentials and Limitations” at the centre for Rural Development
Research & Studies; Faculty of Agriculture; Cairo University; (3-4/5/2003).
7.
Attended a workshop on “Genetic Engineering and Genomics” at Mubark City for
Scientific Research and Technology Application, (5-8 th
of December, 2003).
8.
Presented a lecture under the title of "The molecular bases of potato durable resistance to late blight disease" in the second conference on “Role of Biochemistry in
Environment and Agriculture”, organized in Biochemistry Department, Faculty of
Agriculture, Cairo University, Egypt (February 24-26, 2004).
9.
Presented a poster in the second symposium about “The Scientific research and young researchers” at Faculty of Agriculture, Cairo University, Egypt (22 nd – 23 rd May,
2004).
10.
Presented a paper under the title of "Genes expressed during potato durable resistance to late blight disease" in the sixth national conference on “Biochemistry and Molecular biology and their role in development and environment”, organized by National
Committee for Biochemistry and Molecular biology, Egypt (October 3-5, 2004).
11.
Presented a paper under the title of " Molecular approach toward understanding the basis of potato resistance to Phytophthora infestans " in the first international conference of scientific research and its applications, organized by Cairo University,
Egypt (December 18-20, 2004).
37

12.
Attended a workshop in “Introduction to Bioinformatics” Sponsored by Fulbright commission and Cairo University, at Faculty of Agriculture, Cairo University,
(February 17 th
-27 th
, 2005).
13.
11- Attended a workshop in “Electronic learning” Sponsored by ELEG project. Held at ADEC, 37 rue d’Engwiller , 67350 La Walck , France. (21 st – 24 th March, 2005).
14.
Participated in the organization of the symposium entitled “The role of biotechnology in Egypt and Arab countries” sponsored by JSPS research station in Cairo, on 14 th
May, 2005.
15.
Contributed in the organization of the 4 th
International Conference and Exhibition on
Internet Education, Organized by the Human and Technology Development
Foundation, held at Ramsis Helton, Cairo, Egypt, on 6 th
– 8 th
September, 2005.
16.
Contributed in the organization of the 3 rd
Conference entitled “Recent Technology in
Agriculture” held at Faculty of Agriculture, Cairo University, Egypt (14 th
– 16 th
November, 2005).
17.
Attended a workshop entitled "Strategies and Methods of University Teaching", 26-27
February 2007; 6-8Safar 1428 H.
18.
Contributed in the organization of the 2 nd annual meeting of Saudi Scientific Surgeons
Association. Taif, 12 – 14 June, 2007.
19.
Attended the 1 st
Taif Form for Enhancement of Medical Education, 27-29 May; 2007.
20.
Attended a workshop entitled "Research Skills in the light of informatics and technology", 11-13March 2007; 22-24Safar 1428 H.
21.
Attended the workshop entitled “Developing tests to assess the quality of higher education outcomes” Organized by King Fahd University of Petroleum and Minerals during May 16 -20, 2009.
22.
Attended the workshop entitled “Formulating a Research Proposal” Organized by the
Academic and Training Affairs, College of Medicine, Taif University during 30 th Nov.
– 1 st
Dec. 2010.
23.
Attended the workshop entitled “Microsoft word course for academia” Organized by the Academic and Training Affairs, College of Medicine, Taif University, 25 th
April
2011.
38

24.
Attended the workshop entitled “Writing a Biomedical Research Paper” Organized by the Academic and Training Affairs, College of Medicine, Taif University, 7 th
May
2011.
25.
Attended a workshop entitled "Curriculum and Course Design" Organized by the
Academic and Training Affairs, College of Medicine, Taif University, 26-27 May
2011; 5-6 Rajab 1433 H.
26.
Attended a workshop entitled "Description of Program, Courses & Reporting", 13-14
December 2011; 18-19 al-Muharram 1433 H, Taif University, KSA.
27.
Attended a workshop entitled "Writing a Biomedical Research Paper-Symposium",
31th May-1st June 2011.
28.
Attended a workshop entitled "The ethics, Research and Clinical Applications of Stem
Cells Research"; 3-4 January 2012.
29.
Attended a workshop entitled "Strategic Planning and Management"; 29 April-2 May
2012; 7th-10 Jumada alaKhara 1433 H
30.
Attended a workshop entitled "Use of Technology in Teaching", 14-16 July 2012.
31.
Attended a workshop entitled "Up to date Demonstration and Skills Training". 1st
October 2012.
32.
Attended a workshop entitled "MD consult / First Training session”. 3th October
2012.
33.
Actively participated in a workshop entitled "Radiology updates course" 10-11
November 2013.
34.
Attended a workshop entitled "Time Management in Health Care" 25th September
2013; 19thDhu Qaida 1434 H.
35.
Attended a workshop entitled "Small Groups Discussions" 2 nd
October 2013; 26thDhu
Qaida1434 H.
36.
Actively participated in a workshop entitled"Research Methodology Course" 11-22
May 2013; 1-12 Rajab 1434.
37.
Attended a workshop entitled "Advanced course on Microsoft Excel 2010"9th April
2013; 28 Jumada Alawal 1434 H.
38.
Attended a workshop entitled (Applications of SPSS software in Statistical Analysis)
23 April 2013; 13 Jumada alAkhar 1434 H.
39

39.
Attended a workshop entitled "Assessment and instruction workshop" 13-15 April
2013; 3-5 Jumada alAkhar 1434 H.
40.
Attended a workshop entitled “Advanced course on Microsoft Excel 2010” 20th April
2013; 10thRabiealThani 1434 H
41.
Attended a training program on RTPCR, held at CMMS, Taif University.
42.
Attended a workshop entitled "Improving quality of Assessment" 22 September -1st
October 2013
43.
Attended a workshop entitled "Quality Requirements in e-Learning and Learning
Management systems", 20-21 March 2013; 27-28 Jumada alaKhara 1433 H.
44.
Attended a Symposium for Influence of high altitude on the status of the immune system; 2nd April 2013.
45.
Attended a workshop entitled "Strategic Planning and Management"; 29 April-2 May
2013; 7th-10 Jumada alaKhara 1433 H.
B) Training courses (attended & organized)
1.
Attended a training course on “PCR Technology” at Faculty of Science, Ain Shams
University, Egypt; (23 - 25 th
of November, 1996).
2.
Attended a training course on “Modern Methods in Plant Molecular Biology and
Biochemistry” at Agriculture Analysis Unit, Faculty of Agriculture, Cairo University
& Plant Cell and Tissue Culture Department, Genetic Engineering and Biotechnology
Division, National Research Center; (14 - 24 th
of March, 1998).
3.
Attended computer courses (Word, Excel, PowerPoint, Using the Internet in teaching, using EndNote ….etc) during the Ph.D. degree program at the University of Durham
(1998-2002).
4.
Organized a training course on “Protein analysis: I) Electrophoresis” at Department of
Biochemistry, Faculty of Agriculture, Cairo University, Egypt; (12-15/7/2003).
5.
Organized the first training course on “Molecular biology and nucleic acids” at
Department of Biochemistry, Faculty of Agriculture, Cairo University, Egypt; (6-
9/9/2003).
40

6.
Participated in teaching the first training course on “Analysis of plant secondary metabolites”, which held at Department of Biochemistry, Faculty of Agriculture,
Cairo University, Egypt; (16-19/2/2004).
7.
Organized the second training course on “Molecular biology and nucleic acids” at
Department of Biochemistry, Faculty of Agriculture, Cairo University, Egypt; (5-
10/02/2005).
8.
Organized a training course on “Protein analysis: I) Electrophoresis” at Department of
Biochemistry, Faculty of Agriculture, Cairo University, Egypt; (12-16/02/2005).
9.
Contributed in the organization of the 3 rd
training course on “Molecular biology and nucleic acids” held at Department of Biochemistry, Faculty of Agriculture, Cairo
University, Egypt; (9 th
-13 th
July, 2006).
10.
Trained Cairo University staff members on e-learning through a training program entitled “New teaching methods” (one of the training programmed offered by the
Faculty and leadership Development Project), Cairo university, Egypt. (April –
October, 2005)
11.
Attended eight training programs organized by the FLDP (Faculty and leadership
Development Project), Cairo university, Egypt. (The certificates attached).
12.
Organized a training course on “Protein isolation and separation by electrophoresis” at the Department of Biochemistry, Faculty of Agriculture, Cairo University, Egypt; (4-
6/08/2013).
13.
Attended six training programs organized by the deanship of university development,
Taif University.
14.
A Trainer for a course entitled (Polymerase Chain Reactions Techniques), 27-28
December 2011; 2-3 Safar 1433 H
15.
A Trainer for a course entitled (Polymerase Chain Reactions Techniques), 17-18
October 2011.
16.
Attended a workshop entitled (Microsoft Word for Academia) 25thApril 2011.
17.
Attended a workshop entitled (Writing a Biomedical Research Paper-Symposium),
31th May-1st June 2011.
18.
Attended a workshop entitled (Curriculum and Course Design), 26-27 May 2011; 5-6
Rajab 1433 H.
41

19.
Attended the 1stTaif Fourm for Enhancement of Medical Education 27-29 May 2007.
20.
Attended a workshop entitled (Strategies and Methods of University Teaching), 26-27
February2007; 6-8Safar 1428 H.
21.
Attended a workshop entitled (Research Skills in the light of informatics and technology), 11-13March 2007; 22-24Safar 1428 H.
22.
Attended a workshop entitled (Description of Program, Courses & Reporting), 13-
14December 2011; 18-19 al-Muharram 1433 H, Taif University, KSA.
23.
Attended a workshop entitled (Development Tests to Assess the Quality of Higher
Education Outcomes), 16-20May2009; 21-25Jumada al-Oola 1430 H.
C) Others
1- PI of a research project under the title of “Development of biotechnological strategies for the fight against potato late blight disease” (February, 2004).
2- Member of the plant biotechnology group (plant biotechnology research centre, Faculty of
Agriculture, Cairo University, Egypt). (November, 2002 – October, 2005).
3- Participated in the executive supervision of some of the training programs organized by the
“Faculty and leadership Development Project” at Cairo university, Egypt. (November, 2004 –
October, 2005)
4- Supervisor of the protein analysis research lab, Faculty of Agriculture, Cairo University,
Egypt. (November, 2002 – October, 2005)
5- Member of the committee sponsor for advising the international undergraduate students.
(November, 2002 – October, 2005)
6- Member of the Human and Technology Development Foundation. (2004 – until now)
Awards:
1.
Awarded a complete scholarship to get a Ph.D. degree from U.K. in the field of plant biochemistry and molecular biology (1998-2002).
2.
Awarded the runner up price for the pest poster presented at the University of Durham under the title of "The molecular bases of potato durable resistance" (May, 2001).
3.
Awarded a research fund from Cairo University to continue the research project, which has been started in UK in the area of Molecular Biology under the title of
“Development of biotechnological strategies for the fight against potato late blight disease” (February, 2004).
42

4.
Awarded the first price for the best paper presented in the 6 th
national conference on
“Biochemistry and Molecular biology and Their Role in Development and
Environment”, which organized by National Committee for Biochemistry and
Molecular biology (3 rd
– 5 th
October, 2004)
5.
Awarded a price form Taif University, KSA for the publication in international impacted Journals during 1432/1433.
6.
Awarded a price from Cairo University, Egypt for the publication in international
Impacted Journals during 2012.
7.
Awarded a certificate from, College of medicine and medical sciences, Taif
University, KSA as the best teacher for the year 1431/1432.
8.
Awarded a certificate from, The Egyptian Consulate in Jeddah for the active participation and Cooperation for helping the Egyptian in Taif region, KSA.
Projects Funded by Taif University, Kingdom of Saudi Arabia.
1- Studies on the Protective Potential Effects of Some Natural Products on Aeromonas hydrophila-Induced Sepsis upon Endothelial Cell Line. ( In Vivo and In Vitro Studies).
Academic year 2006-2008. (Co-Principal Investigator)
2- Protective Role of Pomegranate Juice on Fatty Liver in Obesity: An Experimental
Biochemical & Histopathological Study. Project no.
1431 / 875
. (Co-Principal Investigator)
3- Genotyping and Subtyping of Hepatitis C virus isolates from Saudi Arabia. Project no. 1-
1432-1189 (Co-Principal Investigator). Academic year 2010-2011. (Co-Principal
Investigator)
4- Human Viral Diseases Map in Taif, a joint project with Al-Hada Hospital {Approved by
Al-Hada Committee] May 2010- (Co-Principal Investigator).
5- Delineating the inflammatory cascades and pathways during invasion of Aeromonas hydrophila to human intestinal cells causing diarrhea in murine model. Project no. 1-1432-
1243 (Co-Principal Investigator). Academic year 2010-2011
6- Project no. 1- 1432-1190 (Co-Principal Investigator).
43

7- Study the role of Nigilla sativa in the modulation of the testicular toxicity of the colchicine repeated use in adult male albino rats. Project no. 1-434-2323 (Co-Principal Investigator).
Academic year 2012-2013.
Publications
1.
Gomaa AM, Gamal MM, Shehab GMG (2004). Inhibition of In-Vitro protein glycation by Tyrosine, Tryptophane, Aspartic and Glutamic Acids. Bulletin of
Egyptian Society for Physiological Science. 24 (2): 149-162.
2.
Shehab GMG, Croy RRD (2004). Genes expressed during potato durable resistance to late blight disease. Proceeding of the 6 th
national conference on “Biochemistry and
Molecular biology and Their Role in Development and Environment”, organized by
National Committee for Biochemistry and Molecular biology (3 rd
– 5 th
October, 2004).
3.
Gomaa AM, Ahmed Ok, Shehab GMG, Doweidar MM (2005). The effect of dietary fibers on fatty acids in the lens of hypercholesterolemic rats. Bulletin of
Egyptian Society for Physiological Science. 25 (1): 179-194.
4.
Rashed MM, Shaaban M , Shehab GMG, Mahdi SM (2006). Effect of some insecticides on acetylcholine estrase activity in Spodoptera littoralis (BOISD.) larvae.
Egyptian journal of applied Science, 21 (4B): 418-426.
5.
EI-Sayed KA, Shehab GMG (2006). Tissue Protein Pattern of Biomphalaria
Alexandrina and some Schistosomiasis Non Vector Snails Using SDS-PAGE. Journal of Biological Chemistry and Environmental Sciences 1(2): 355-367.
6.
Ahmed OK, Elshihy OM, Shehab GMG, Gomaa AM (2006).
Antioxidant and hepatoprotective activity of Ginkgo biloba leave extracts.
Bulletin of Egyptian Society for Physiological Science 26 (2): 15-30.
7.
Shehab GMG, Ahmed OK, Elshihy OM, Mohamed MA (2007).
Molecular approach toward understanding the basis of potato resistance to Phytophthora infestans . Bull. Fac. Agric., Cairo Univ., 58: 188 - 197.
8.
Shehab GMG (2007).
Nitric oxide increases the activity of the antioxidative enzymes and phenylalanine ammonia-lyase and induces the expression of new isozymes during potato-Phytophthora infestans interaction. J. Biol. Chem. Environ. Sci. 2(3): 93-111
9.
Shehab GMG, Ahmed OK, El-Beltagi HES (2007) . Nitric Oxide Prevents Rice
Callus ( Oryza sativa L.) from Drought Stress. Bull. Fac. Agric., Cairo Univ. 58(4):
256-265
10.
Shehab GMG, (2007). The expression profile of five potato genes during the resistant mechanism to Phytophthora infestans
. J. Biol. Chem. Environ. Sci. 2(3): 113-127
11.
Abdel-Samad A, Shehab GMG, Elshihy OM and Salamma RA (2008):
Biochemical studies on production of some secondary metabolites in Ecballium elaterium using tissue culture techniques. J. Biol. Chem. Environ. Sci., 3(3): 287-302.
44

12.
Mohamed MK, Shehab GMG, Mahmoud AA, Amer AA (2008): Influence of different plant growth regulators on callus induction and flavonoid contents in Alfalfa
( Medicago sative L.). J. of Agric. Sc. Mansoura Univ., 33 (6): 3951 – 3965.
13.
Naguibm AM, Shehab GMG, Saker MM, Shaffaa HA M (2009): Direct
Regeneration of Egyptian Cottons (Gossypium barbadense L) Shoots from
Cotyledonary nodes and Shoot Apex. J. Biol. Chem. Environ. Sci., 4(4):151-165.
14.
Ahmed OK, Shehab GMG, Romeilah RM (2009): Effects of Rosmarinus Officinalis
Extract on Induced Nitric Oxide and Stimulated Proinflammatory Mediators Via-
Bacterial Lipopolysaccharide (LPS). Australian Journal of Basic and Applied
Sciences, 3(2): 1223-1233.
15.
Abdel-Moneim AS, Shehab GMG, Abu-Elsaad AS (2011).
Molecular evolution of the six internal genes of H5N1 equine influenza A virus. Archives of Virology. 156
(7): 1257-1262.
16.
El-Rashedy AH, Belal SK, Osman HA, Shehab GMG, (2011). Protective Role of
Pomegranate on Fatty Liver in Obesity: An Experimental Chemical &
Histopathological Study. The Egyptian Journal of Hospital Medicine, 43: 162 – 172.
17.
Abdel-Moneim AS, Bamaga MS, Shehab GMG, Abu-Elsaad AS, Farahat FM
(2012). HCV Infection Among Saudi Population: High Prevalence of Genotype 4 and
Increased Viral Clearance Rate. PLoS ONE 7(1): e29781.
18.
Abd El-Razek FH, Mohamed E, Shehab GMG, Hassan AA, Gomaa AM (2012).
Effects of Cactus Pear ( Opuntia ficus indica ) Juice on Oxidative Stress in Diabetic
Cataract Rats. Saudi Journal of health Sciences, 1(1): 23-29.
19.
Farah HS, Al- Atoom AA, Shehab GMG (2012). Explanation of the Decrease in
Alkaline phosphatase (ALP) Activity in Hemolysed Blood Samples from the Clinical
Point of View: In vitro study. Jordan Journal of Biological Sciences, 5(1): 31-36.
20.
El-Badrawy E, Ibrahim Z S, Abedel Aziz A, Kamel MM, Shehab GM and Kamal
A (2013). Association of Vitamin D Receptors Genes Polymorphism ( Apa I , and Taq
I ) with type 1 diabetes in Saudi Arabia (KSA). Life Science Journal, 10(3): 1555-1562
(ISSN: 1097-8135).
21.
Ahmed, OK, Shehab, GMG and Abdel-Mobdy, Y E (2013).
Semi-Modified Carrot
Diet Alleviates the Toxicity Effects of Dimethoate in Albino Rats. Aust. J. Basic &
Appl. Sci., 7(14): 554-561.
22.
Shehab GMG , Ahmed OK and Ahmad MA (2013). Jatropha curcas (Linn) Seed
Ethanolic Extract Possess Antifungal Activity against Phytophthora infestans and
Stimulates Potato Resistant Response. J. Biol. Chem. Research. 30 (2): 929-941.
23.
Shehab, GMG and Osman, HH, (2014).
Nigella Sativa Seeds Ameliorate the
Hepatotoxicity and Nephrotoxicity of Colchicine Prolonged Use in Albino Rats: a
Biochemical and Histopathological Study. Aust. J. Basic & Appl. Sci., 8(2): 362-370.
45

24.
Shehab GMG, Elshama SS, El-Kenawy AE, Osman HH (2014). Nigella sativa seeds ameliorate the testicular toxicity of colchicine repeated use in adult male albino rats: a biochemical and histopathological study. J. Biol. Chem. Research. 31(1): 146-
163.
25.
El-Assal S, El-Awady M A, El-Tarras A, and Shehab GMG (2014). Assessing the genetic relationship of taif rose with some rose genotypes (rosa sp.) based on random amplified polymorphic dna, inter simple sequence repeat and simple sequence repeat markers. American Journal of Biochemistry and Biotechnology 10 (1): 88-98.
46

1- Personal Information:

Name:

DOB:

Social Status:

Contact :

Scientific Degree:

Specialist Area:
Eman Salah Abdel-Reheem
19/9/1970
Married + 4
+966-551233078
Associated Professor
Physiology
2- Research Activities:
2.1 Scientific Articles:
1Abdel-Reheim, E. S. and Zaki, A. R (2014): The protection of purslane against reproductive toxicity of carbamazepine. Reprod. Toxicol., under revision and printing.
2Abdel-Reheim, E. S.
; Ahmed, O. A.; Ashour, B. M.; and Ahmed, W. H. (2014): The chemopreventive effects of onion and garlic oils against valproic acid-induced toxicity. Exp. Toxicol. Pathol., under revision and printing.
3Abdel-Reheim, E. S.
; Hafez, H. S.; Shehab, G. M. (2014): Mitigating therapeutic activity of taurine: as antioxidant and inducer for DJ1 and Pink1 expression in rotenone Parkinson’s disease model. under revision
4Abdel-Reheim, E. S.
; Abdel-Hafeez, H. A.; Mahmoud, B. M. and Abd-Allah, E. N.
(2014): Effect of food additive (monosodium glutamate and sodium nitrite) on some biochemical parameters in albino rats. International Journal of Bioassays, ISSN:
2278-778X, 3260-3273
5Abdel-Reheim, E. S. and Ali, A. H. (2013): Ameliorative effect of cinnamaldehyde and ellagic acid on hematological alterations associated with pathophysiology of gestational diabetes mellitus in albino rats. Egypt. J. Zool. DOI:10.12816/0003299,
60:375-396.
47

6Abdel-Reheim, E. S.
; Abdel-Hafeez, H. H.; Hozayen, W. G. and Mahmud, N. A.
(2013): Neurophysiological Study on the Effects of Using the Artificial Food Colours;
Tartrazine and Sunset yellow. Minufia J. Agr. Res.; 38 (4): 879-894.
7Abdel- Hameed ; A., Soliman, H. S.
and Abdel-Reheim, E. S. (2013): Protective
Role of Wheat Germ Oil in Clozapine-Induced Oxidative Stress and Biochemical
Alterations in Liver of male albino rats. J.Amer.Sci.;9 (1): 268-274.
8Abdel-Reheim, E. S.; Ahmed, R. R. and Mohamed, E. T. (2009): Modulatory Effect of Pumpkin Seed Oil on Pilocarpine-Model of Epilepsy Compared to Topirmate as A
Common Antiepileptic Drug. J. Egypt. Ger. Soc. Zool., 58 (c):1-15.
9Abdel-Reheim, E. S.
(2008): Physiological and Biochemical Studies of Melatonin
Effect on The Fertility of Epileptic Rats. J. Egypt. Ger. Soc. Zool., 57 (A): 235-263.
10Abdel-Reheim, E. S.
and Ahmed, R. R. (2008): Alterations of 7, 12-Dimethyl
Benz (a) anthracene-Induced Gonadal Toxicity in Rats by The Action of Some Dietary
Substances; Hickory Liquid Smoke and Diallyl Disulfide. J. Egypt. Ger. Soc. Zool.,
11Abdel-Reheim, E. S. and Abdel-Tawab, S. M. (2008): Physiological, biochemical and Histological Effects of Coumarin in Streptzotocin Diabetic Rats. J. Egypt. Ger.
Soc. Zool., 56 (A): 329-376.
12Abdel-Reheim, E. S.
; Helmi, H. and Abdel-Moneim, A. A. (2008): The Effect of
Diallyl Disulphide and Hickory Liquid Smoke on Toxicity Induced by 7, 12-
Dimethylbenz( a )anthracene in Male Abino Rats. J. Egypt. Ger. Soc. Zool., 56 (A):
121-155.
13Gad, M. A. and Abdel-Reheim, E. S.
(2008): Phagocytic Activity, Electrolytes
Balance and Oxidative Stress Monitoring for the Effect of Diallyl Disulphide on
Mercuric Chloride-Toxicity. J. Egypt. Ger. Soc. Zool., 57 (A): 125-150.
14Abdel-Reheim, E. S.; Ahmed, O. M.; Ashour, M. B. and Abdel-Tawab, S. M.
(2007): Biochemical Study on the Effect of Rosiglitazone and Various Plant
Constituents on Streptozotocin-Induced Diabetic Rats. J. Egypt. Ger. Soc. Zool., 54
(A): 309-339.
15Ahmed , O. M.; Abdel-Reheim, E. S.; Ashour, M. B. and Abdel-Tawab, S. M.
(2007): Evaluation of Anti-hyperglycemic Effect of Some Medicinal Plant Ingredients
48

and Their Probable Mechanisms on Streptozotocin-Induced Diabetic Rats. J. Egypt.
Ger. Soc. Zool., 53 (A): 53-100.
16Abdel-Reheim, E. S. (2006): Biochemical Effects of Cuminum nigrum and Panax ginseng on Streptozotocin Diabetic Rats. J. Egypt. Ger. Soc. Zool., 51 (A): 423-453.
17Ahmed, O. M. and Abdel-Reheim, E. S. (2005): Hypolipidemic, Proteogenic and
Kidney Functions Effects of Curcumin and Esculetin in Diabetic Rats. J. Egypt. Ger.
Soc. Zool., 47 (A): 193-219.
18Abdel-Moneim, A. A.; Ashour, M. B. and Abdel-Reheim, E. S. (2004): Study on
Some Organosulpher Compounds on Streptozotocin Diabetic Albino Rats, 2-
Hypolipidemic and Proteogenic Effects. J. Egypt. Ger. Soc. Zool., 43 (A): 367-398.
19Abdel-Moneim, A. A.; Ashour, M. B. and Abdel-Reheim, E. S. (2003): Study on
Some Organosulpher Compounds on Streptozotocin Diabetic Albino Rats, 1-
Hypoglycemic Effects. J. Egypt. Ger. Soc. Zool., 41 (A): 195-225.
20Abdel-Moneim, A. A.; El-Feki, M. A. and Abdel-Reheim, E. S. (1997): Effect of
Nigella Sativa Oil, Fish Oil and Gliclazide on Alloxan Diabetic Rats, 1-Biochemical and Histopathological Studies. J. Egypt. Ger. Soc. Zool., 23 (A): 237-265.
21El-Feki, M. A.; Radwan, Z. A.; Abdel-Moneim, A. A. and Abdel-Reheim, E. S.
(1997): Effect of Nigella Sativa Oil and Fish Oil on Alloxan Diabetic Rats, 2-
Immunological Studies. J. Egypt. Ger. Soc. Zool., 23 (A): 161-186.
22Abdel-Moneim, A. A.; El-Feki, M. A.; Radwan, Z. A. and Abdel-Reheim, E. S.
(1997): Effect of Nigella Sativa Oil and Fish Oil on Alloxan Diabetic Rats, 3-
Haematological Studies. J. Union Arab Biol. Cairo, 8 (A) Zoology, 411-430.
:ةيملعلا تايعمجلا يف ةكراشملا 2.2
.ًاقباس ناويحلا ملعل ةينامللأا ةيرصملا ةيعمجلا 
.برعلا نييجولويبلا داحتإ 
.ناويحلا ملع ةيعمج 
.ةيعيبطلا مومسلل ةيرصملا ةيعمجلا 
.ةيئيبلا مومسلل ةيرصملا ةيعمجلا 
49

.ةيبيرجتلا ايجولويبلل ةيرصملا ةبعمج 
.
ةيئيزجلا ايجولويبلا و ةيويحلا ءابميكلل ةيرصملا ةيعمجلا 
.ةيناطرسلا مارولأا تلالادل ةيرصملا ةيعمجلا 
:
ةيلكلاب نيثحابلا بابش تارمتؤم يف ةكراشملا -
2.
3
2014.
/ليربا عبارلاو 2013 /ليربا ثلاثلاو 2012 /ليربا يناثلا ليرمتؤملل دادعلإا ةنجل يف ةكراشملا
:ةيملعلا تاودنلاو تارمتؤملل دادعلإا وأ يف ةكراشملا 2.4
تارمتؤؤؤملا ةؤؤعاقب ةؤؤيئيزجلا اؤؤيجولويبلاو عوؤؤيحلا ءاؤؤيميكلل ةيرؤؤصملا ةؤؤيعمجلل عونؤؤسلا يؤؤملعلا رمتؤؤؤملا *
.)رتسوب ميدقت( 2014 /ويام 2 1 سمش نيع ةعماجب
.
2014 /رياربف 25 20 ةرهاقلا ةعماجب تارمتؤملا زكرمب ةيبيرجتلا ايجولويبلل رشاعلا يلودلا رمتؤملا *
.
2013 /رياربف 27 23 ةرهاقلا ةعماجب تارمتؤملا زكرمب ةيبيرجتلا ايجولويبلل عساتلا ي لودلا رمتؤملا *
2 لؤيربا 30 فيوؤس ينب ةعماجب ناويحلا ملعل ةينامللأا ةيرصملا ةيعمجلل رشع عساتلا يلودلا رمتؤملا *
.
2011 /ويام
8 7 ةؤؤؤيجلاعلا اؤؤؤهتار يثأتو ةؤؤؤيبطلا تاؤؤؤتابنلا نؤؤؤع فيوؤؤؤس يؤؤؤنب ةؤؤؤعماج ةلديؤؤؤصلا ةؤؤؤيلكب ةؤؤؤماقملا ةودؤؤؤنلا *
.
2010 /ليربا
.
2009 /فيوس ينب ةعماجب ةيئيبلا مومسلل ةيرصملا ةيعمجلل سماخلا يلودلا رمتؤملا *
.
2006 ربمسيد 20 18 ةيعيبطلا مومسلل ةيرصملا ةيعمجلل ىناثلا ىملعلا رمتؤملا*
.
2006 /ربمفون 30 25 برعلا نييجولويبلا داحتلإ رشع ثلاثلا يلودلا رمتؤملا *
.
2006 / ليربا 20 18 فيوس ينب ةعماجب ةيئيبلا مومسلل ةيرصملا ةيعمجلل يناثلا رمتؤملا *
.
2006 ليربأ 13 12 تايمسلا مولعل ةيرصملا ةيعمجلل نورشعلاو ىداحلا ىملعلا رمتؤملا*
2006 سرام 9 – 3 نم ناويحلا ملعل ةينامللأا ةيرصملا ةيعمجلل رشع سداسلا ىلودلا رمتؤملا
ربمؤسيد 12 ءاودو ءاد ةردؤخملا تاؤتابنلا نؤع ةؤيعيبطلا مومؤسلل ةيرؤصملا ةؤيعمجلل ةيناثلا ةيملع لا هودنلا *
2005.
:ةيثحبلا تاعورشملا يف ةكراشملا وأ يلع لوصحلا 2.5
:ناونعب فيوس ينب ةعماج تاساردلاو ثوحبلا زكرم نم لومملا عورشملا يف ىسيئرلا ثحابلا
."
NATURAL PRODUCTS AGAINST GESTATIONAL DIABETES
"
E.P.
40000 ليومتب
50

:ةيثحبلا ةيلمعلاب ةقلاع اهلو اهيلع لصاحلا تارودلا 2.6
.
2014 /ليربا ىف فيوس ينب ةعماج ةلديصلا ةيلكب يملعلا ثحبلا مييقت ةرود
ةرتفلا ىف فيوس ينب ةعماج بادلآا ةيلك قحلمب " Bio-informatics " ةيويحلا ةيتامولعملا ةرود
.
2014 / 3 / 13 12
زكرمب ةدقعنملا ؛لازتخلإاو ةدسكلأا تاراشإو ميظنتب صاخلا رشنلا ليلحت؛ ناونعب لمع ةشرود
.
2013 /ليربا فيوس ينب مولعب ةئيبلا ثاحباو ليلاحتلا
.
2013 /سرام ؛ عمتجملا ةضهنو ةيمنت لجأ نم ةعانصلاب ةيملعلا ثوحبلا طبر ؛ ناونعب لمع ةشرو
.
2013 /رياربف ؛ةثيدحلا ةيملعلا تاهاجتلإا يف ونانلا ايجولونكت رود؛ ناونعب لمع ةشرو
.
2012 /ويلوي ؛
SPSS
؛ مادختسإب يجولويبلا ءاصحلإا نع بيردت ةرود
)
IBRO
( خملا ثاحبلأ ةيلودلا ةمظنملا ةياعر تحت ةيبصعلا ةعانملل ةيناثلا ةيلودلا ةرودلا
ةيليعمسلإاب سيوسلا ةانق ةعماج بطلا ةيلكو ) NGE ( ةيبصعلا مولعلل ةيرصملا ةعومجملا عمنواعتلابو
.
2008 /ربمسيد 3 ىلإ ربمفون 24 نم ةرتفلا يف
13 نم ةرتفلا يف ةيردنكسلإا ةعماج ثوحبلا و ايلعلا تاساردلا زكرمب نيتوربلا لصف تايساسأ ةرود
.
2008 /ليربأ 16 ىلإ
:ةيملعلا لئاسرلا يلع فارشلإا يف ةكراشملا 2.7
)مهحنم مت 2 ( هاروتكد لئاسر 4 و )مهحنم مت 6 (ريتسجام ةلاسر 1 5 ىل ع فارشلأا*
: يميلعتلا طاشنلا 2
:سيردتلا 3.1
.)ءايميك -
.)ءايميك -
:)مولعلا ةيلكل( سويرولاكبلا ةلحرم *
ناويح( ةعبارلا ةقرفلل تلاضعلاو لقنلا ايجولويسف سيردت
.)صاخ ناويح( ةعبارلا هقرفلا بلاطل ناويحلا كولس ملع ررقم سيردت


ناويح( ةدمتعم تاعاس
نيمدقتملا بلاطلاو
– ةثلاثلا ةقرفلا بلاطل يئاذغلا ليثمتلاو صاصتملإا سي
صاخ ناويح ةبعش سويرولاكبلا ةبلط ثاحبأ ىلع فارشلإا
ردت
ىف ةكراشملا


ءايميك ي
.ةيملعلا تارمتؤملل
جولويب يتبعشو يجولويبلا ةبعش( ىلولأا هقرفلا بلاطل يجولويسفلا ملع ررقم سيردت 
.)ءايزيف ءايميكو
51

) يجولويبلا ةبعش( ىلولأا هقرفلل بلاطل ناويحلا ملع تايساسأ ررقم سيردت
ءايميكو ءايميك يجولويب يتبعش( ىلولأا ةقرفلا بلاطل ناويحلا ملع لخدم ررقم سيردت
.ءايميك ناويحو صاخ ناويح -
.)ءايزيف
ةعبارلا ةقرفلل يجولويسفلا ةدامل يلمعلا فارشلإا
.ىلولأا ةقرفلا بلاطل )حيرشتلا ملع( ةيلمعلا سوردلا ىلع فارشلاا




.يجولويسفلا مسق ريتسجاملا عديهمت بلاطل سفنتلاو جارخلإاو يبصعلا رزآتلا ررقم سيردت
.ةلماكتملا
:)مولعلا ةيلكل( ايلعلا تاساردلا ةلحرم*
ةيئيزجلا ايجولويسفلا مسق ريتسجاملا عديهمت بلاطل باصعلأا مولع ررقم سيردت


-
.ماعلا ميلعتلا -
.ماعلا ميلعتلا
.ماعلا ميلعتلل ةيبرتلا ةيلك -
:يرخلأا تايكلا *
ةثلاثلا هقرفلا بلاطل ءامصلا ددغلا ملع ررقم سيردت 
ةيبرتلا ةيلك )ءايزيف
ةيبرتلا ةيلك
.يساسلأا ميلعتلل ةيبرتلا ةيلك
.يساسلأا ميلعتلل ةيبرتلا ةيلك
.بادلآا ةيلك -
ةيناثلا ةقرفلا بلاطو ةيبرتلا ةيلك -
ءايميك( ىلولأا هقرفلا بلاطل يجولويسفلا ملع ررقم سيردت
)يجولويب( ىلولأا هقرفلا بلاطل ناويحلا كولس ملع ررقم سيردت
سفنلا ملع مسق
-
-
ةثلاثلا هقرفلا بلاطل يجولويسفلا ملع ررقم سيردت
ىلولأا هقرفلا بلاطل يجولويسفلا ملع ررقم سيردت
ةيناثلا هقرفلا بلاطل ءامصلا ددغلا ملع ررقم سيردت
ةثلاثلاو ىلولأا قرفلا بلاطل هيلمعلا سوردلا ىلع فارشلاا






.بادلآا ةيلك
52

Date and place of birth : July,23,1968 ( Dakahlia, Egypt)
Nationality: Egyptian
Marital status:
Title:
Married
Mobile:
E.mail:
Assistant Professor of Microbiology, College of Medicine and Medical Sciences, Taif University
0581168832 imadmohamed@edu.tu.sa
imad200040@yahoo.com
QUALIFICATIONS

Ph D of Microbiology, Leipzig University, Germany

Master of Microbiology, Zagazig University, Egypt
2004
1996

B.V.Sc, Faculty of Vet. Medicine, Zagazig University, Egypt 1992
EMPLOYMENTS

2008 - until now: Assistant Professor of Microbiology, College of Medicine and Medical
Sciences, Taif University, Saudia Arabia

2004 - 2008 : Lecturer of Microbiology, Faculty of Vet. Medicine, Zagazig University,
Egypt

1999 - 2004: Ph D student in Institute of Bacteriology and Mycology, Leipzig
University, Germany

1996 - 1999: Assistant Lecturer of Microbiology, Faculty of Vet. Medicine, Zagazig
University, Egypt

1996 - 1999:
University, Egypt
Demonstrator of Microbiology, Faculty of Vet. Medicine, Zagazig

Arabic :

English :

Germany:
Mother Language
Very good spoken and written
Very good spoken and written

Microsoft Windows, Word / Excel /Power Point and Internet

Member of Registration Unit and Formatting tables

Coordinator of Skill Lab Committee
53


Member of Evaluation Committee for Staff College

Member of Internship Committee

Member of Academic Advising Committee

Member of Coordination Committee

Member of Examination Unit

Reviewer of Saudi Journal for Health Sciences

SGD training Workshop, College of Medicine & Medical Sciences,2008 – 2009

MCQs implementation Workshop, College of Medicine & Medical Sciences, 2009 – 2010

First Annual Retreat Workshop, College of Medicine & Medical Sciences, 2010

Polymerase Chain Reaction Workshop, College of Medicine & Medical Sciences,
Eppendorf Company,2010

Monash University Stem Cell Workshop, College of Medicine & Medical Sciences, 2010

Medical Education Workshop (Curriculum and Course Design), AWALIV International
Hotel, Taif, 2011

Objective Structured Clinical Examination-1 (OSCE-1) Workshop, College of Medicine and Medical Sciences, 2012

Objective Structured Clinical Examination-1 Seminar, College of Medicine and Medical
Sciences, 2012

Objective Structured Clinical Examination-1 Meeting, AWALIV International Hotel, Taif ,
2012

KIDS WITHOUT AIDS Meeting, College of Medicine and Medical Sciences, 2012

Characterization of Programs and Courses and the Preparation of Annual Reports
Workshop, 2012

Objective Structured Clinical Examination-2 (OSCE-1) Workshop, College of Medicine and Medical Sciences, 2012

Accreditation Seminar, College of Medicine and Medical Sciences, 2012

E-Learning Seminar, College of Medicine and Medical Sciences, 2012

Medical Curricular Advances in the Kingdom of Saudi Arabia Workshop, InterContinental
Hotel, Taif, 2012

Up To Date Demonstration & Skills Training in October 1 st
, 2012, College Of Medicine and Medical Sciences

MD Consult/First Consult Training Session , Taif University, College of Medicine,
03/10/2013

Orientation to medical education issues in college of medicine, 15th October 2012

Active Learning Techniques and Self-Directed Learning Awaliv Hotel, 2-3/12/2012

Developing Research Abilities (Idea Formulation and Research Management)Taif
University, King Abduaziz City for Science and Technology, 21/11/2012

Time Management in Health Care 25 th
sep. 2013 – 19 th
Dhul Qaida 1434

Improving Quality of Assessment 29, 30 Sep. and 1 st
October 2013

Strategic Planning in Higher Education Institutes 6 th
Feb. 2014 (6 th
Rabie Thani 1435)

Shared Lecture 19-20 th
Feb. 2014 (19-20 th
Rabie Thani 1435)

SWOT analysis 26 th
Feb. 2014 (26 th
Rabie Thani 1435 )
54


El-Bakery, R. M. (2009): Some studies on bacterial diseases of rabbits. M.V.Sc., Faculty of vet. Medicine, Zagazig Uni., Egypt

El-Baz, H. A. (2009): Fungal causes of respirtory diseases in chickens. M.V.Sc., Faculty of vet. Medicine, Zagazig Uni., Egypt

Mohamed I. A. A.
(1996): Study on some bacterial causes of the early chick mortalities in
Shakia province. M.V.Sc., Faculty of vet. Medicine, Zagazig University., Egypt

Mohamed I., Schrödl W., Hafez H. and Krüger M.
(2004): Investigations the probiotic effects of Escherichia coli strain 1917 after oral infection with Salmonella Enteritidis in chickens. Proc. Soc. Nutr. Physiol. (13) 144, 58. Tagung vom 09.– 11.03. in Göttingen,
Germany

Mohamed I. A. A. (2004): Influence of probiotic strain Escherichia coli Nissle 1917 on experimental infections with Salmonella Enteritidis PT4 in chickens. Ph D of Microbiology,
Bacteriology and Mycology Institute, Faculty of Vet. Medicine, Leipzig University,
Germany

Soliman, M. H. and Mohamed, I. A. A. (2007): Effect of chromium and essential oils mix supplementation on growth performance, serum metabolites, lymphoid organs, cecal microflora and immune response of broiler chickens. Mansoura, Vet. Med. J. Issn 1110-
7219) Vol. 9, No. 1 PP 1-24

Gad, G. N., Eleiwa, N.Z. and Mohamed, I. A. A. (2008): Evaluation of the concurrent use of
Beta 1,3 Glucan and Enrofloxacin in chicks experimentally infected with Salmonella enteritidis. 9th Vet. Med. Zagazig Conference, (20 – 22) Auguset ) Port-Said, A. R. Egypt

Bayoumie, H.A. and Mohamed, I. A. A. (2009): Laboratory evaluation of selected disinfectants on Gumboro disease virus. Assiut Vet. Med. J. Vol. 55 No. 120 P (231 – 242)

Abuelsaad AS1, Mohamed I, Allam G, Al-Solumani AA. (2013): Antimicrobial and immunomodulating activities of hesperidin and ellagic acid against diarrheic Aeromonas hydrophila in a murine model. Life Sci. 93(20):714-22.

MICROBIOLY COURSE, FACULTY OF PHARMACY, 1429-1431H

BIOTECHNOLOGY COURSE, FACULTY OF PHARMACY, 1430-1431H

PATHO-PHYSIOLOGY COURSE, FACULTY OF PHARMACY, 1430-1431H

MEDICAL BIOLOGY COURSE, FACULTY OF PHARMACY, 1430-1431H

MEDICAL MICROBIOLOGY COURSE, FACULTY OF MEDICINE, 1429-1434H

IMMUNOLOGY BLOCK, FACULTY OF MEDICINE, 1429-1434H

CNS BLOCK, FACULTY OF MEDICINE, 1429-1434H

SPECIAL SENSES BLOCK, FACULTY OF MEDICINE, 1429-1434H

RESPIRATORY BLOCK, FACULTY OF MEDICINE, 1429-1434H

CVS BLOCK, FACULTY OF MEDICINE, 1429-1434H

GIT BLOCK, FACULTY OF MEDICINE, 1430-1434H

COORDINATOR OF GIT BLOCK, FACULTY OF MEDICINE, 1430-1434H
55


KIDNEY BLOCK, FACULTY OF MEDICINE, 1430-1434H

OB-GYN BLOCK, FACULTY OF MEDICINE, 1430-1434H

ENDOCRINE BLOCK, FACULTY OF MEDICINE, 1430-1434H

COORDINATOR OF ENDOCRINE BLOCK, FACULTY OF MEDICINE, 1430-1434H

LOCOMOTION BLOCK, FACULTY OF MEDICINE, 1430-1434H

DERMATOLOGY BLOCK, FACULTY OF MEDICINE, 1430-1434H

COORDINATOR OF PSYCHOLOGY BLOCK, FACULTY OF MEDICINE, 1429-
1430H

COORDINATOR OF CVS BLOCK, FACULTY OF MEDICINE, 1430-1431H

BIOLOGY COURSE, 1
ST
YEAR, FACULTY OF MEDICINE, 1433-1434H

COORDINATOR OF PEDIATRICS BLOCK, FACULTY OF MEDICINE, 1433-1435H

COORDINATOR OF ORTHOPEDIC BLOCK, FACULTY OF MEDICINE, 1433-
1434H

COORDINATOR OF OSCE BLOCK, FACULTY OF MEDICINE, 1432-1435H

COORDINATOR OF 5
TH
YEAR, FACULTY OF MEDICINE, 1433-1435H

MEMBER OF REGISTRATION UN IT, 1432 – 1435H

MEMBER OF INTERN UNIT, 1432 – 1435H
 MEMBER OF STUDENT’S ACTIVITY COMMITTEE, 1435H

MEMBER OF EVALUATION OF ACADEMIC PERFORMANCE, 1432-1435H

MEMBER OF INTERNATION COOPERATION UNIT, 1435H

SUPPORT UNIT COORDINATOR AND TEACHING ASSISTANTS AND
LECTURERS, 1435H
 MOLECULAR DETECTION AND CHARACTERIZATION OF
VIRUSES THAT CAUSE GASTROENTERITIS IN CHILDREN IN
TAIF
فئاطلاب لافطلأا يف ءاعملأاو ةدعملا باهتللا ةببسملا تاسوريفلا فيصوتو يئيزجلا فشكلا
1 435 3331 (Accepted) .
1

LINK BETWEEN INFLAMMATION CYTOKINES AND ANTI-

INFLAMMATORY CYTOKINES AND VITAMIN D IN CHILDREN
AND YOUNG PEOPLE WITH DIABETES TYPE I
1 435 3134
لافطلأا يف د نيماتيف و تاباهتللإل ةداضملا تانيكوتيسلاو تاباهتللإا تانيكوتيس نيب طابت رلاا
لولاا عونلا نم يركسلا ءادب نوباصملا بابشلاو

,
56

Name
ةلئاعلا
ضوع
First
Ezzat Mohamed
Schwarzspanierstrasse 17, A-1090
Vienna, Austria
----------------------
00431427762532
004316769169041 ezzat.awad@meduniwien.ac.at
EGYPTIAN
Vienna
Medicine
Vascular Biology and Thrombosis Research
Associated Prof.
Cell and Molecular Biology
Inflammation, Tumor Biology, Vascular
Biology, Thrombosis, Angiogenesis
دجلا
دومحم
Second
بلأا
دمحم
Third
لولأا
تزع
Family
مسلاا
Mahmoud Awad
: (Complete Mailing Address)
لماكلاب ةلسارملا ناونع
: () فتاهلا مقر
: (Home) لزنم (Work) لمع
(Mobile) لاوجلا
: E-Mail) ينورتكللإا ديربلا
: ( Nationality ) ةيسنجلا
: ( University ) ةعماجلا
: (College) ةيلكلا
(Department) مسقلا
: ( Academic Title) ةيملعلا ةبترملا
: ( Major Field ) ماعلا صصختلا
ةيثحبلا ةطشنلأاو قيقدلا صصختلا
(Specialization and Research Activities)
ةلئاعلا
ملاع
دجلا بلأا
دمحأ ىلع
لولأا
دمحأ مسلاا
57

First Second Third Family
Name Ahmed Aly
ضايرلا دوعس كلملا ةعماج مولعلا ةيلك ناويحلا ملع مسق
ةيدوعسلا ةيبرعلا ةكلمملا
00966591093790 allam1081981@ yahoo.com
EGYPTIAN
ةيدوعسلا ةيبرعلا ةكلمملا ضايرلا دوعس كلملا ةعماج
مولعلا
ناويحلا ملع
كراشم ذاتسأ
هضارمأو ىبصعلا زاهجلا – نراقملا حيرشتلا ملع
Cell and Tissue culture, neurodegenerative assays, Fluorescence microscopy, Histology and
Histochemistry.
Ahmed Allam
لماكلاب ةلسارملا ناونع
: (Complete Mailing Address)
: () فتاهلا مقر
: (Home) لزنم (Work) لمع
(Mobile) لاوجلا
: E-Mail) ينورتكللإا ديربلا
: ( Nationality ) ةيسنجلا
: ( University )
:
ةعماجلا
(College) ةيلكلا
(Department) مسقلا
: ( Academic Title) ةيملعلا ةبترملا
: ( Major Field ) ماعلا صصختلا
ةي ثحبلا ةطشنلأاو قيقدلا صصختلا
(Specialization and Research Activities)
58

ةلئاعلا
نيسح يلع
سيوسلا ةانق ةعماج مولعلا ةيلك ناويحلا ملع مسق
ةيبرعلا رصم ةيروهمج
Name
First
Hani
00201154149052 hs_hafez@yahoo.it
دجلا
ظفاح
Second
Sayed
EGYPTIAN
ةيبرعلا رصم ةيروهمج سيوسلا ةانق ةعماج
مولعلا
ناويحلا ملع
كراشم ذاتسأ
هضارمأو ىبصعلا زاهجلا – نراقملا حيرشتلا ملع
بلأا
ديس
لولأا
ىناه مسلاا
Third Family
Hafez
Aly
Hussein
: (Complete Mailing Address)
لماكلاب ةلسارملا ناونع
: () فتاهلا مقر
: (Home) لزنم (Work) لمع
(Mobile) لاوجلا
: E-Mail) ينورتكللإا ديربلا
: ( Nationality ) ةيسنجلا
: ( University ) ةعماجلا
: (College) ةيلكلا
(Department) مسقلا
: ( Academic Title) ةيملعلا ةبترملا
: ( Major Field ) ماعلا صصختلا
Cell and Tissue culture, neurodegenerative assays,
Fluorescence microscopy, Histology and
Histochemistry.
ةيثحبلا ةطشنلأاو قيقدلا صصختلا
(Specialization and Research Activities)
59

Name
ةلئاعلا
رديح
First
Doaa
دجلا
بيرغ
بلأا
دمحا
Second
Ahmad
Third
Ghareeb
لولأا
ءاعد
Family
Hadier
مسلاا
لماكلاب ةلسارملا ناونع
: (Complete Mailing Address)
ةيردنكسلاا – مولع ةيويحلا ءاييمي كلا مسق
Biochemistry Department, faculty of Science,
Alexandria university
035449649
00201277291144
, doaa.ahmed@alex d.ghareeb@yahoo.com
sci.edu.eg
ةيرصم
ةيردنكسلاا
مولعلا
ةيويحلا ءايميكلا
كراشم ذاتسا
ةيويح ءايميك
ةيبطلا ةيويحلا ايجولونكتو ةعانملاو ةيعيبطلا داوملا
: () فتاهلا مقر
: (Home) لزنم (Work) لمع
(Mobile) لاوجلا
: E-Mail) ينورتكللإا ديربلا
: ( Nationality ) ةيسنجلا
: ( University ) ةعماجلا
: (College) ةيلكلا
(Department) مسقلا
: ( Academic Title) ةيملعلا ةبترم لا
: ( Major Field ) ماعلا صصختلا
ةيثحبلا ةطشنلأاو قيقدلا صصختلا
(Specialization and Research Activities)
60

Name
ةلئاعلا
لاعلا دبع
First
Hanaa
دجلا
نسح
Second
ALi
ةروصنملا ةعماج مولعلا ةيلك ناويحلا ملع مسق
ةيبرعلا رصم ةيروهمج
00201001059907 drhanaahassan@yahoo.com
EGYPTIAN
ةيبرعلا رصم ةيروهمج ةروصنملا ةعماج
مولعلا
ناويحلا ملع
ناويحلا ملع مسف سيئرو ذاتسأ
ىجولويسفلا ملع
Medical Physiology & Nutritional Therapy
بلأا
ىلع
Third
ل ولأا
ءانه
Family
مسلاا
Hassan Abdelaal
: (Complete Mailing Address)
لماكلاب ةلسارملا ناونع
: () فتاهل ا مقر
: (Home) لزنم (Work) لمع
(Mobile) لاوجلا
: E-Mail) ينورتكللإا ديربلا
: ( Nationality ) ةيسنجلا
: ( University ) ةعماجلا
: (College) ةيلكلا
(Department) مسقلا
: ( Academic Title) ةيملعلا ةبترملا
: ( Major Field ) ماعلا صصختلا
ةيثحبلا ةطشنلأاو قيقدلا صصختلا
(Specialization and Research Activities)
61