UCSF Lab Standard Operating Procedure
Chemical Process, Name or Hazard Class:
2,4-D Butyric Acid
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Type of SOP:
☐ Process
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☒Hazardous Chemical
☐ Hazardous Class
Purpose
2,4-D butyric acid is a highly toxic chemical. This SOP provides information about its hazards and how to
mitigate them through proper controls, handling, and storage. It is an herbicide used to selectively kill both
annual and perennial broadleaf plants that are considered weeds.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 94-82-6
Class: Highly Toxic
Molecular Formula: C10H10Cl2O3
Form (physical state): Solid
Melting point: 248 - 248 °F
Potential Hazards/Toxicity
Oral LD50 [Rat]: 700 mg/kg
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Inhalation: May be harmful if inhaled. May cause respiratory tract irritation.
Ingestion: Harmful if swallowed.
2,4-D Butyric Acid
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Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
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Skin Contact: Harmful if absorbed through skin. May cause skin irritation.
Eye Contact: May cause eye irritation.
Chronic Exposure: May cause cancer.
Engineering Controls
 All operations involving 2,4-D butyric acid should be carried out in a certified chemical fume hood,
glovebox, or a ducted Biosafety cabinet to keep airborne level below recommended exposure limits.
 Chemical fume hoods used as containment areas for particularly hazardous chemicals must have a
face velocity of 100 feet/min, averaged over the face of the hood and must be certified annually.
 Laboratory rooms must be at negative pressure with respect to the corridors and external
environment. The laboratory/room door must be kept closed at all times.
 Vacuum lines are to be protected by HEPA (high efficiency particulate air) filters or higher efficiency
scrubbers.
Personal Protective Equipment (PPE)
Respiratory Protection
If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by
EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/)
Hand Protection
Nitrile gloves are recommended.
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are
compatible with 2,4-D butyric acid .
Refer to glove selection chart from the links below:
http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
OR
http://www.allsafetyproducts.biz/page/74172
OR
http://www.showabestglove.com/site/default.aspx
OR
http://www.mapaglove.com/
Eye Protection
ANSI approved safety glasses.
Skin and Body Protection
Lab coats should be worn. These laboratory coats must be appropriately sized for the individual and be
buttoned to their full length. Laboratory coat sleeves must be of a sufficient length to prevent skin exposure
while wearing gloves. Full length pants and close-toed shoes must be worn at all times by all individuals that
are occupying the laboratory area. The area of skin between the shoe and ankle should not be exposed.
Hygiene Measures
Wash thoroughly after handling. Wash hands before eating. Remove contaminated clothing and wash before
reuse.
First Aid Procedures
If inhaled
Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get
medical attention.
2,4-D Butyric Acid
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Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
In case of skin contact
Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing
and shoes. Get medical attention immediately. Wash clothing before reuse. Thoroughly clean shoes before
reuse. Contaminated work clothes should be laundered by individuals who have been informed of the
hazards of exposure to this substance.
In case of eye contact
Immediately flush eyes with plenty of water for at least 15 minutes, lifting lower and upper eyelids
occasionally. Get medical attention.
If swallowed
Never give anything by mouth to an unconscious person. Rinse mouth with water. Get medical attention.
Special Handling and Storage Requirements
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All work with 2,4-D butyric acid is to be done in a “designated area” in order to keep contamination
to a minimum.
Any persons in this area are required to wear personal protective equipment. Safety shower and eye
wash stations should be easily accessible where 2,4-D butyric acid is used.
All laboratory equipment (such as beakers, pipettes, etc.) used in the designated area are to be
labeled and are not to be removed from the area without first being decontaminated.
Store away from incompatible chemicals including strong oxidizers.
Spill and Accident Procedure
Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only)
Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area.
Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent
material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal
protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear
plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-2068522 (SFGH only) for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency
shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no
further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-4761300 during work hours, or 9-911 during non-working hours and weekends.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for
15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency
Department. If no further discomfort is experienced, have the SDS ready and contact Poison
Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct
2,4-D Butyric Acid
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Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)
supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working
hours and weekends.
Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-8857580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday
7:30 a.m. - 4:00 p.m. (except Holidays).
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the
affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane
exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick
nurse by dialing 415-353-7842 (STIC).
Decontamination/Waste Disposal Procedure
Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as
hazardous waste.
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html
Protocol/Procedure
Quantities covered by this SOP:
______ (g , ml) to _______ (g, ml)
Temperature range covered by this SOP:
__ °C – __ °C
General Overview and Purpose:
Enter the experimental purpose
Procedure:
Enter experimental procedure. You can copy procedure from your lab notebook or from literature.
NOTE
Any deviation from this SOP requires approval from the Principal Investigator.
2,4-D Butyric Acid
4
Date: Click here to enter a date.
Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)