Supplementary Information
Supplementary Table 1. OPCRIT items.
The 48 clinical OPCRIT items included in principal component analysis.
Item number
Item
17
Bizarre behavior
18
Catatonia
19
Excessive activity
20
Reckless activity
21
Distractibility
22
Reduced need for sleep
23
Agitated activity
24
Slowed activity
25
Loss of energy/tiredness
26
Speech difficult to understand
27
Incoherent
28
Positive formal thought disorder
29
Negative formal thought disorder
30
Pressured speech
31
Thoughts racing
32
Restricted affect
33
Blunted affect
34
Inappropriate affect
35
Elevated mood
36
Irritable mood
37
Dysphoria
39
Loss of pleasure
41
Poor concentration
42
Excessive self reproach
43
Suicidal ideation
53
Increased sociability
54
Persecutory delusions
55
Well organized delusions
56
Increased self esteem
57
Grandiose delusions
58
Delusion of influence
59
Bizarre delusions
60
Widespread delusions
61
Delusion of passivity
62
Primary delusional perception
63
Other primary delusional perception
66
Thought insertion
67
Thought withdrawal
68
Thought broadcast
69
Delusions of guilt
70
Delusions of poverty
71
Nihilistic delusions
72
Thought echo
73
Third person auditory hallucinations
74
Running commentary voices
75
Abusive/accusatory/persecutory voices
76
Other (non affective) auditory voices
77
Non-affective hallucination in any modality
Supplementary Table 2. Factor solution.
Twelve-factor solution. Item loadings after varimax rotation.
Item
Bizarre behavior
Catatonia
Excessive activity
Reckless activity
Distractibility
Reduced need for sleep
Agitated activity
Slowed activity
Loss of energy/tiredness
Speech difficult to understand
Incoherent
Positive formal thought disorder
Negative formal thought disorder
Pressured speech
Thought racing
Restricted affect
Blunted affect
Inappropriate affect
Elevated mood
Irritable mood
Dysphoria
Loss of pleasure
Poor concentration
Excessive self reproach
Suicidal ideation
Increased sociability
Persecutory delusions
Well organized delusions
Increased self esteem
Grandiose delusions
Delusion of influence
Depression
-.060
Mania
.106
Delusions
.504
Depersonalization
.098
Grandiose Delusions
.079
.080
-.027
.041
.053
-.020
.065
.726
.036
.033
.048
-.052
.566
-.022
.080
.033
.416
-.005
-.059
.035
.603
.045
Voices
.097
Agitation
.176
Disorganization
.227
.109
-.027
.102
.042
.114
.042
.102
.045
-.113
.026
.201
-.036
.217
.044
-.019
.107
-.041
.074
Other Hallucinations
.105
Negative Mood Delusions
-.006
Catatonia
.422
Negative Symptoms
.053
.098
.065
.701
.015
-.023
-.011
.006
-.028
-.244
.010
.074
-.061
.174
.024
.024
.167
-.099
.098
.082
.092
-.034
.167
.219
.069
.044
-.142
.051
.554
-.058
-.031
.040
.131
.114
.658
-.025
-.060
.037
.041
.010
.110
.143
-.007
.014
.040
.025
.854
-.002
-.049
-.005
.016
-.017
-.016
.047
-.054
-.029
.002
.025
-.087
.130
.207
.003
.034
-.025
.195
.589
.151
.036
.241
.010
.004
.082
.193
.030
-.018
-.051
.687
.262
.210
.030
.001
.002
-.041
.169
.259
.062
.111
.035
.175
.677
-.028
-.007
.125
-.070
.159
-.032
.032
-.002
.038
.119
-.041
.676
-.015
.088
-.026
.193
.113
.678
.030
.033
.096
-.002
.098
.105
.095
-.095
-.133
-.117
-.051
.615
.056
.005
.171
-.037
.123
.119
.111
-.082
-.066
.086
.242
-.039
.046
-.023
.082
.065
.251
.110
-.028
-.013
-.076
.671
.039
-.032
-.006
.083
-.077
-.027
-.085
.045
.042
.104
.105
.702
-.075
.018
.056
.054
.259
-.006
.308
.065
-.042
.090
.510
.050
.100
.668
.083
.044
-.107
.015
.014
.022
-.003
.005
.015
-.007
-.045
.202
-.034
-.063
.384
.001
.402
.109
-.001
.015
.024
.162
.803
.036
-.023
-.034
.085
.014
-.037
-.018
-.071
-.076
.019
.044
.863
.012
-.014
-.011
.001
-.004
-.035
.018
-.044
-.045
-.044
.017
.604
.132
.069
.014
-.042
-.052
.046
-.002
.129
.029
-.067
.084
.542
.103
.071
.003
-.053
-.039
-.060
-.232
.138
.155
.137
.038
.445
.025
.060
.037
-.134
.058
.162
.001
-.119
.164
-.028
.001
.049
.309
.057
.024
.306
.073
.496
.061
-.070
-.109
.167
-.130
-.025
.057
.340
.212
.477
.132
.042
.102
.190
.084
.021
.031
.010
.053
.734
.089
.108
.006
.044
.043
.060
.013
-.017
.040
-.001
.389
.055
.043
.604
.023
.120
-.060
-.030
.005
-.042
.044
-.035
.150
.206
.061
.685
.034
-.030
.022
.064
.034
.096
-.058
-.004
.029
.256
.324
.520
.101
-.165
.166
.176
.046
.162
-.058
Bizarre delusions
Widespread delusions
Delusion of passivity
Primary delusional perception
Other primary delusional perception
Thought insertion
Thought withdrawal
Thought broadcast
Delusions of guilt
Delusions of poverty
Nihilistic delusions
Thought echo
Third person auditory hallucinations
Running commentary voices
Abusive/accusatory/persecutory voices
Other (non affective) auditory voices
Non-affective hallucination in any modality
-.015
.072
.714
.100
.091
.041
.107
.002
.076
-.084
.201
.077
.022
.040
.743
.071
.127
.113
-.009
.199
-.001
.065
-.066
-.051
.065
.017
.265
.511
.241
.031
.382
.029
.173
.065
-.165
-.082
.091
.027
.444
.165
.303
.097
.347
.114
.167
.070
-.079
-.258
.083
.023
.354
.111
.361
.025
.267
.221
.137
.154
-.044
-.254
-.048
.066
.119
.775
.066
.074
.015
.053
-.008
.038
.009
.052
.027
.012
.125
.729
.041
.077
.052
-.098
-.105
.015
.094
.098
.052
.029
.043
.639
.119
-.018
-.084
.054
.324
.021
.048
-.036
.109
-.039
.142
.173
.077
.084
.010
-.006
-.026
.636
.095
.099
.049
.048
-.118
.042
.027
.057
.042
.021
-.033
.672
-.109
.077
-.054
-.072
.035
-.126
.012
-.002
-.011
.081
.027
.712
.163
-.076
-.024
.034
-.046
.330
-.074
.354
.078
.144
.115
-.056
.073
-.186
.007
-.002
.066
.085
.066
.713
-.078
.004
.026
.000
.196
.011
-.012
-.012
.068
.042
.090
.808
.080
.019
.215
.012
-.066
.022
-.014
.005
.095
.010
.021
.803
.023
.061
.049
.160
-.013
.033
-.030
.078
.111
.119
.092
.154
-.034
.072
.754
-.010
.008
.033
-.039
.048
.136
.070
.112
.230
.209
-.025
.679
-.044
.139
-.033
Bold type indicates the item loadings that contribute to each factor
Supplementary Table 3. Distribution of the OPCRIT items for the factor dimension
“Negative Mood Delusions” in the German sample.
Factor Dimension
Depression
Mania
Delusions
Depersonalization
Grandiose Delusions
Voices
Agitation
Disorganization
Other Hallucinations
Negative Mood Delusions
Catatonia
Negative Symptoms
Rotation Sums of Squared Loadings
Total
% of Variance
Cumulative %
3,634
7,571
7,571
3,165
6,594
14,165
2,791
5,815
19,980
2,256
4,700
24,680
2,224
4,633
29,312
2,154
4,487
33,799
2,074
4,321
38,120
1,747
3,640
41,761
1,649
3,435
45,196
1,582
3,295
48,490
1,376
2,866
51,357
1,345
2,801
54,158
Supplementary Table 4. Correlation matrix for the derived factor dimensions.
Depression
1
Mania
-.033
Delusions
-.009
Grandiose Delusions
.025
Depersonalization
-.002
Voices
-.001
Agitation
-.018
1
-.006
-0.114
-.036
-.034
-0.087
1
.029
0.144
0.071
1
0.074
1
Disorganization
.057
Other Hallucinations
0.076
Negative Mood Delusions
.031
Catatonia
.022
Negative Symptoms
-.006
.005
.017
.027
-.052
.001
0.151
0.176
0.102
0.089
0.1
.018
.050
.010
.021
.044
.019
0.064
-.052
0.111
.038
0.11
0.179
.049
.047
0.067
1
.051
.038
0.184
0.123
0.171
.024
1
0.107
0.114
.058
0.108
-0.064
1
.038
0.082
0.083
0.069
1
.012
.036
.026
1
0.123
0.078
1
.061
Depression
Mania
Delusions
Grandiose Delusions
Depersonalization
Voices
Agitation
Disorganization
Other Hallucinations
Negative Mood Delusions
Catatonia
1
Negative Symptoms
Bold type indicates significant correlations.
Supplementary Table 5. Distribution of the OPCRIT items for the factor
dimension “Negative Mood Delusions” in the German sample.
Delusions of guilt
Delusions of poverty
Nihilistic delusions
Delusions of guilt
Delusions of poverty
Nihilistic delusions
N=55
N=15
N=13
N=15
N=17
N= 7
N=13
N= 7
N=12
OPCRIT, Operational Criteria Checklist for Psychotic Illness.
Supplementary Table 6. Association between rs9875793 and the OPCRIT items
of the factor dimension “Negative Mood Delusions” in the German GWAS
sample.
SNP data
Association data
German sample
allelic χ2 model
Marker
rs9875793
rs9875793
rs9875793
Chromosome Alleles
3
3
3
G/A
G/A
G/A
OPCRIT item
Delusions of guilt
Delusions of poverty
Nihilistic delusions
PG
0.0008
n.s.
0.023
MAF
trait present
trait absent
0.26
0.23
0.28
0.16
0.16
0.16
OR
1.87
1.60
2.03
Alleles. minor/major allele. Minor allele refers to dbSNP build129. and was
determined in each analysis in patients with- and without the trait; SNP,
single nucleotide polymorphism; MAF, minor allele frequency; OR, odds
ratio; referring to the minor allele; OPCRIT, Operational Criteria Checklist
for Psychotic Illness; n.s., non significant.
Supplementary Table 7. Association between rs9875793 and a categorical
diagnosis of BD and “Negative Mood Delusions” in the German GWAS sample
and in the European American follow-up sample.
SNP data
Association data
German sample
allelic χ2 model
Marker
rs9875793
rs9875793
rs9875793
rs9875793
rs9875793
rs9875793
Chromosome Alleles
3
3
3
3
3
3
samples
G/A
G/A
G/A
G/A
G/A
G/A
G+
GG
EA+
EAEA
Ncases
PG
"Negative
Mood
Delusions";
89 0.0001
838 0.0001
927 0.013
286 0.028
988 n.s.
1274 n.s.
SNP,
single
1.93
0.72
0.82
1.27
1.21
1.15
nucleotide
polymorphism; MAF, minor allele frequency; OR, odds ratio; referring to
the minor allele; n.s., non significant.
cases
controls
0.27
0.12
0.14
0.17
0.16
0.16
0.16
0.16
0.16
0.14
0.14
0.14
OR
G, German; EA, European American; + with "Negative Mood Delusions"; without
MAF