Supplemental Table 3 Distribution of radioactivity 24 hours after feeding [ 35S]GSH to petioles of
Col-0 leaves that were pretreated for 24 h with Spodoptera littoralis larvae.
Glucosinolates
Cys
GSH
Total extracta
4MSB
I3M
% incorporationb
1.7
7. 9
19.0
3.4
4.4
Specific activity
(cpm/µmol)c,d
3.11 ± 0.45
1.53 ± 0.14
n.d.
0.89 ± 0.26e
1.34 ± 0.16e
a
desulfo-glucosinolates, as prepared for HPLC analysis
percentage of the total applied radioactivity (7.15 x 10 6 cpm) incorporated
c
values are x106
d
data are means ± SE of 3 samples
e
the mass of GS produced de novo within the 24 h feeding period was used to calculate the specific
activity
n.d., not determined
b
Supplemental Table 4 Distribution of radioactivity 28 hours after feeding [ 35S]Cysteine to petioles of
Col-0 leaves that were pretreated for 48 h with Spodoptera littoralis larvae and preincubated 4.5 h in
the different treatment solutions.
Glucosinolates
Treatment
Cys
%
b
total extracta
GSH
s.a.
c
%
s.a.
%
s.a.
4MSB
%
s.a.
I3M
d
%
s.a.d
Control
0.4
6.49
2.0
2.86
24.8
n.d.
5.9
2.65
5.1
5.14
BSOe
0.9
2.98
1.2
3.79
14.3
n.d.
3.1
2.58
2.3
2.74
GSH
0.9
1.30
2.3
0.78
18.9
n.d.
3.4
11.50
4.8
2.44
BSO + GSH
1.2
1.39
2.0
0.82
13.3
n.d.
3.1
2.90
2.0
1.38
a
desulfo-glucosinolates, as prepared for HPLC analysis
percentage of the total applied radioactivity (48.8 x 10 6 cpm) incorporated
c
s.a., specific activity (cpm/µmol), the values are x 10 6
d
the mass of GS produced de novo within the 28 h feeding period was used to calculate the specific
activity
e
buthionine-sulfoximine, specific inhibitor of glutathione synthase
n.d., not determined
b