Isosensitist Medium
40mM Tris pH 9.5
RNAse
Hazardous Substances Policy - Assessment
DNAse
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
Prof N Brown
Assessor
Mrs Rita Godfrey
Assessment Number*
1991
Date of Assessment
28.1.03
Signature
Signature
Notes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
List names where possible
Post Docs, Post graduates and Technicians in T101
3
ACTIVITY ASSESSED
4
MATERIALS INVOLVED
NAME
T101
Membrane Protein Preparation
AMOUNT
max/stored
Attach copies of data sheet(s)
HAZARD
RISK PHRASES
Isosensitist Medium
500g
Irritant
40mM Tris pH 9.5
1 litre
NSH
RNAse
50µl
DNAse
50µl
Avoid contact and
inhalation
Avoid contact and
inhalation
8% Chaps
1 litre
Hazard
unknown
Hazard
unknown
NSH
Solubilization Solution
8M Urea
2M Thiourea
2% Chaps
2% SB3-10
40mM Tris
20ml
Category 3
Carcinogen
R40 Possible risk of
irreversible effects
.R63 Possible risk of harm
to the unborn child.
R51/53 Toxic to aquatic
organisms, may cause
long-term adverse effects
in the aquatic environment.
Ready to use
solubilization solution i.e
As above plus per 400ul:
0.5% IPG buffer
2mM TBP
100mM DTT
few grains bromophenol
blue
IPG buffer
Dangerous for
the
environment
R37 Irritating to the
respiratory system
As above
400ul
As above
1ml
NSH
HAZDAT NO***
BIOSCIENCESNO***
DTT
25g
Irritant
R36/38 Irritating to the
eyes and respiratory
system
TBP
20ml
Corrosive
Highly
Flammable
Harmful
R34 Causes burns
R17 Spontaneously
flammable in air.
R21/22 Harmful in contact
with skin and if swallowed.
Bromophenol Blue
10g
NSH
5
1340
INTENDED USE**
Give brief details and attach protocol/instructions
Isolation of the proteins from bacterial membrane.
See protocol attached
6
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
Pregnant women need to take extra precautions when handling thiourea as this can harm the unborn child.
There is a fire risk from the TBP
There is a risk of exposure to the isosensitist media, DTT and TBP when weighing or measuring out.
There is a risk to the environment from the disposal of thiourea
The risk from handling RNase and DNase is unknown
7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
Control measures are necessary when using thiourea and TBP. The risk to the unborn child can be reduced by
the pregnant mother avoiding handling thiourea or getting someone else to do that stage of the method for them.
Good chemical practice should eliminate any risks from the others
As the risk from RNAse and DNAse is unknown it would be wise to handle them with caution.
8
CONTROL MEASURES
Additional to Good Chemical Practice
Avoid creating a dust when weighing out DTT and isosensitist media.
Keep TBP away from sources of ignition and keep the stock in a nitrogen chamber. Once it is in the solution it is
safer.
Wear gloves when handling RNAse and DNAse.
Wear protective clothing and gloves when handling thiourea and TBP
Thiourea should be weighed out in fume hood.
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
Instruction in the use of the nitrogen chamber for the first time.
10
MONITORING
Performance of control measures,
Check the nitrogen chamber and the fume hood are working correctly before use.
Check integrity of gloves during use.
Personal exposure
Health Surveillance
Occupational Health must be notified before
commencing this work due to the presence of a
carcinogen – thiourea Tel: 42695
11
WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Thiourea and all solutions containing it must not go down the sink. It should be stored in a non-halogenated waste
solvent bottle until full and then taken down to Stores for specialist disposal.
Solutions containing TBP can be washed down the sink with plenty of water as the concentration of TBP and the
volumes used are so small. Any waste stock TBP would have to be disposed of as specialist waste via the
Stores.
All other compounds can be washed down the sink with plenty of water.
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
January 2008
13
EMERGENCY ACTION
TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Thiourea - sweep up avoiding creating a dust and transfer to a suitable labelled container. Take to Stores for
specialist disposal.
TBP – use paper towels if spillage is small or absorbant powder from the spill kit to mop up and transfer to a
suitable labelled container and take to Stores for specialist disposal.
TO PROTECT PERSONNEL Evacuation, protection for personnel involved in clean-up, Special First Aid
Evacuate the area. Wear protective clothing, gloves and goggles or a face shield
Clean-up/decontamination
TO RENDER SITE OF EMERGENCY SAFE
The site should be washed with water and detergent and mopped up with paper towels. The paper towels used
for thiourea should be placed in a sealed bag and taken to Stores for specialist disposal.
CONTACT
Prof N. Brown
PHONE
45467
10.10.00
*
**
***
Prefix T is used for Teaching Assessment Number.
Please include amount of chemicals used and how.
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.