Leong Pubs, p. 1
Jo-Ann C. Leong
Director, Hawai’i Institute of Marine Biology
PUBLICATIONS
a. Peer-Reviewed Journal Articles
Garapin, A., J. Leong, L. Fanshier, W. Levinson, and J. M. Bishop. 1971. Identification of virusspecific RNA in cells infected with Rous sarcoma virus (RSV). Biochem. Biophys. Res.
Commun. 45(5):919-925.
Garapin, A., L. Fanshier, J. Leong, J. Jackson, W. Levinson, and J. M. Bishop. 1971. The DNA
polymerase of RSV: Kinetics of DNA synthesis and specificity of the products. J. Virology
8(2):227-232.
Leong, J., W. Levinson, and J. M. Bishop. 1972. Synchronization of Rous sarcoma virus
production in chick embryo cells. Virology 47:133-141.
Leong, J., H. Smith, and H. Goodman. 1976. Post-transcriptional processing of viral RNA in
simian virus 40 transformed cells. Virology 71:355-360.
Smith, H., S. Turner, J. Leong, and P. Rigby. 1976. Effect of passage in culture on a clone of
Balb/c3T3 cells transformed by SV40. J. Virology 19(1):146-153.
Garfin, D., J. Leong, and H. Goodman. 1976. The EcoRI site of simian virus 40 deoxyribonucleic
acid: Nucleotide sequences of the minus and plus strands. Biochem. Biophys. Res. Commun.
68(2):369-374.
Leong, J., J. Kane, O. Olesko, and J. Levy. 1977. Antigen-specific nonimmunoglobulin factor
that neutralizes xenotropic virus is associated with mouse serum lipoproteins. Proc. Natl. Acad.
Sci. USA 74(1):276-280.
Hedrick, R. P., J. Leong, and J. L. Fryer. 1978. Persistent infection in salmonid fish cells with
infectious pancreatic necrosis virus (IPNV). J. Fish Diseases 1:297-308. Ore. Ag. Exper.
Station Tech. Paper No. 4794.
Nelson, J., J. Leong, and J. Levy. 1978. Normal placentas contain RNA-directed DNA
polymerase activity like that in viruses. Proc. Natl. Acad. Sci. USA 75(12):6263-6267.
Grinnell, B. and J. Leong. 1979. Recovery and concentration of IPN virus in water. J. Fish.
Res. Bd. Can. 36(11):1405-1408. Ore. Ag. Exp. Station Tech. Paper No. 5135.
Scott, J., J. Fendrick, and J. Leong. 1980. Growth of infectious hematopoietic necrosis virus in
mosquito and fish cell lines. Wasman J. of Biology 38(1,2):21-29.
Fryer, J. L., B. B. McCain, and J. C. Leong. 1980. A cell line derived from rainbow trout (Salmo
gairdneri) hepatoma. Proc. Jap. Soc. Fish Pathol. Tokyo 15(3/4):33-40.
Engelking, M. and J. Leong. 1981. IHNV persistently infects chinook salmon embryo cells.
Virology 109:47-58.
Leong, J., J. Fendrick, A. Lee, and S. Youngman. 1981. Effect of polybrene on the infectivity of
infectious hematopoietic necrosis virus in tissue culture. J. Fish Diseases 4(4):335-344.
Leong Pubs, p. 2
Nelson, J., J. Levy, and J. C. Leong. 1981. Human placentas contain a specific inhibitor of DNA
polymerase. Proc. Natl. Acad. Sci. USA 78:1670-1674.
Levy, J., S. Barrett, J. Leong, and E. Dirksen. 1981. Transformation of macrophages from hybrid
mice by simian virus 40. J. of Reticuloendothelial Society 29(1):34-46.
Leong, J., Y. L. Hsu, H. M. Engelking, and D. Mulcahy. 1981. Strains of infectious hematopoietic
necrosis (IHN) virus may be identified by structural protein differences. Develop. Biol. Standard.
49:43-55.
Fendrick, J. L., W. J. Groberg, Jr., and J. C. Leong. 1982. Comparison of five piscine cell lines to
the isolation of infectious hematopoietic necrosis virus from naturally infected salmonid fishes. J.
of Fish Diseases 5:77-85.
Leong, J. C., S. D. Wood, A. O. Lyford, and J. A. Levy. 1984. Purification of a specific inhibitor of
reverse transcriptase from human placenta. International Journal of Cancer 33(4):435-439.
Leong, J. C., J. A. Nelson, and J. A. Levy. 1984. Optimal conditions for detection of reverse
transcriptase activity in human placentas. Biochemica et Biophysica Acta 82:441-445.
Kurath, G., and J. C. Leong. 1985. Characterization of infectious hematopoietic necrosis virus
mRNA species reveals a nonvirion rhabdovirus protein. J. Virology 53(2):462-468. Ore. Ag.
Exper. Station Tech. Paper No. 7323.
Kurath, G., K. Ahern, G. Pearson, and J. C. Leong. 1985. Molecular cloning of the six mRNA
species of IHNV, a fish rhabdovirus, and gene order determination by R-loop mapping. J.
Virology 53(2):469-476. Ore. Ag. Exper. Station Tech. Paper No. 7324.
Hsu, Y. L. and J. C. Leong. 1985. A comparison of detection methods for infectious
hematopoietic necrosis virus. J. Fish Diseases 8:1-12. Ore. Ag. Exper. Station Tech. Paper No.
7166.
Hsu, Y. L., H. M. Engelking, and J. C. Leong. 1985. Analysis of the quantity and synthesis of the
virion proteins of infectious hematopoietic necrosis virus. Japanese Journal of Fish Pathology
20:331-338.
Huang, M., S. Manning, M. Warner, E. Stephens, and J. Leong. 1986. A physical map of the
viral genome for infectious pancreatic necrosis virus Sp: Analysis of cell-free translation products
derived from viral cDNA clones. J. Virology 60(3):1002-1011. Ore. Ag. Exper. Station Tech.
Paper No. 8018.
Hsu, Y. L., H. M. Engelking, and J. C. Leong. 1986. Occurrence of different types of infectious
hematopoietic necrosis virus in fish. Applied and Environ. Microbiology 50(6):1353-1361.
Koener, J. F., C. W. Passavant, G. Kurath, and J. Leong. 1987. Nucleotide sequence of a cDNA
clone carrying the glycoprotein gene of infectious hematopoietic necrosis virus (IHNV), a fish
rhabdovirus. J. Virology 61(5):1342-1349. Ore. Ag. Exper. Station Tech. Paper No. 8137.
Kurath, G. and J. Leong. 1987. Transcription in vitro of infectious hematopoietic necrosis virus, a
fish rhabdovirus. J. Gen. Virology 68:1767-1771. Ore. Ag. Exper. Station Tech. Paper No.
6755.
Gilmore, R. D., H. M. Engelking, D. S. Manning, and J. Leong. 1988. Expression in Escherichia
coli of an epitope of the glycoprotein of infectious hematopoietic necrosis virus against viral
challenge. Bio/Technology 6:295-300. Ore. Ag. Exper. Station Tech. Paper No. 8321.
Leong Pubs, p. 3
Stewart, T. A., N. B. Hecht, P. Hollingshead, P. A. Johnson, J. C. Leong, and S. L. Pitts. 1988.
Haploid-specific transcription of protamine-myc and protamine-Tag fusion genes in transgenic
mice. Mol. Cell Biol. 8(4):1748-1755.
Gilmore, R. D., Jr. and J. C. Leong. 1988. The nucleocapsid gene of infectious hematopoietic
necrosis virus, a fish rhabdovirus. Virology 167(2):644-648. Ore. Ag. Exper. Station Tech.
Paper No. 8530.
Engelking, H. M. and J. C. Leong. 1989. The glycoprotein of infectious hematopoietic
necrosis virus elicits neutralizing antibody and protective responses. Virus Research 3:213230. Ore. Ag. Exper. Station Tech. Paper No. 8742.
Engelking, H. M. and J. C. Leong. 1989. Glycoprotein from infectious hematopoietic
necrosis virus (IHNV) induces protective immunity against five IHNV types. J. Aquatic
Animal Health 1(4):291-300. Ore. Ag. Exper. Station Tech. Paper Nos. 8595 and 8596.
Feyereisen-Koener, J. and J. C. Leong. 1990. Expression of the glycoprotein gene from a
fish rhabdovirus using baculovirus vectors. J. Virology 64(1):428-430. Ore. Agri. Exper.
Station Tech Paper No. 8960.
Manning, D. S., C. L. Mason, and J. C. Leong. 1990. Cell-free translational analysis of the
processing of infectious pancreatic necrosis virus polyprotein. Virology 179:9-15. Ore. Ag.
Exper. Station Tech. Paper No. 8885.
Manning, D. S. and J. C. Leong. 1990. Expression in Escherichia coli of the large genomic
segment of infectious pancreatic necrosis virus. Virology 179:16-25. Ore. Ag. Exper.
Station Tech. Paper No. 8884.
Duncan, R., C. L. Mason, E. Nagy, J. Leong, and P. Dobos. 1991. Sequence analysis of
infectious pancreatic necrosis virus genome segment B and its encoded VP1 protein: a
putative RNA-dependent RNA polymerase lacking the Gly-Asp-Asp motif. Virology 181:541552.
Xu, L., D. V. Mourich, S. Ristow, J. Arnzen, and J. C. Leong. 1991. Epitope mapping and
characterization of the infectious hematopoietic necrosis virus glycoprotein using fusion
proteins synthesized in Escherichia coli. J. Virology 65(3):1611-1615. Ore. Ag. Exper.
Station Tech. Paper No. 9340.
Engelking, H. M., J. Harry, and J. C. Leong. 1991. Comparison of representative strains of
infectious hematopoietic necrosis virus by serological neutralization and cross-protection
assays. Applied & Environ. Microbiol. 57(5):1372-1378. Ore. Ag. Exper. Station Tech.
Paper No. 9495.
Leong, J. and C. B. Munn. 1991. Potential uses of recombinant DNA in the development of
fish vaccines. Bull. European Assn. of Fish Pathologists 11(1):30-40.
Oberg, L. A., J. Wirkkula, D. Mourich, and J. C. Leong. 1991. Bacterially expressed
nucleoprotein of infectious hematopoietic necrosis virus augments protective immunity
induced by the glycoprotein vaccine in fish. J. Virology 65(8):4486-4489. Ore. Ag. Exper.
Station Tech. Paper. No. 9169.
Bootland, L. M. and J. C. Leong. 1992. Staphylococcal coagglutination - a rapid method of
identifying infectious hematopoietic necrosis virus. Applied & Environ. Microbiol. 58(1): 613. Ore. Ag. Exper. Station Tech. Paper. No. 9686.
Leong Pubs, p. 4
Drolet, B., J. S. Rohovec, and J.C. Leong. 1993. Serological identification of infectious
hematopoietic necrosis virus in fixed tissue culture cells by alkaline phosphatase
immunocytochemistry. J. Aquatic Animal Health 5(4):265-269. Ore. Ag. Exper. Station
Tech. Paper. No. 10,056
Leong, J.C. 1993. Molecular and biotechnological approaches for fish vaccines. Current
Opinion in Biotechnology, London, vol. 4(3):286-293. Ore. Ag. Exper. Station Tech. Paper.
No. 10,169.
Bootland, L. M., H. V. Lorz, J. S. Rohovec, and J. C. Leong. 1994. Experimental infection of
brook trout (Salvelinus fontinalis) with infectious hematopoietic necrosis virus types 1 and 2.
J. Aquatic Animal Health 6(2):144-148. Ore. Ag. Exper. Station Tech. Paper. No. 10,261.
Hansen, J., J. C. Leong, and S. Kaattari. 1994. Complete Nucleotide sequence of a rainbow
trout cDNA encoding a membrane-bound form of immunoglobulin heavy chain. J. of Mol.
Immunol. 31(6):499-501. Ore. Ag. Exper. Station Tech. Paper. No. 10,400.
Drolet, B. S., J. S. Rohovec, and J. C. Leong. 1994. The route of entry and progression of
infectious hematopoietic necrosis virus in Oncorhynchus mykiss: a sequential
immunohistochemical study. J. Fish. Diseases 17:337-347. Ore. Ag. Exper. Station Tech.
Paper. No. 10,303.
Kim, C. H., J. R. Winton, and J. C. Leong. 1994. Neutralization-resistant variants of
Infectious Hematopoietic Necrosis Virus have altered virulence and tissue tropism. J.
Virology 68(12):8447-8453. Ore. Ag. Exper. Station Tech. Paper No. 10,575.
Chiou, P.-W., B. Drolet, and J. C. Leong. 1995. PCR amplification of infectious
hematopoietic necrosis virus RNA extracted from fixed and embedded fish tissue. J. Aquat.
Animal Health 7(1):9-15. Ore. Ag. Exper. Station Tech. Paper. No. 10533.
Drolet, B. S., P. W. Chiou, J. Heidel, and J. C. Leong. 1995. Detection of truncated virus
particles in a persistent RNA virus infection in vitro. J. Virology 4:2140-2147. Ore. Ag.
Exper. Station Tech. Paper No. 10,602.
Trobridge, G., and J. C. Leong. 1995. Characterization of a rainbow trout Mx gene. J. of
Interferon and Cytokine Research 15:691-702. Ore. Ag. Exper. Station Tech. Paper No.
10,679.
Mourich, D.V., J. Hansen, and J. C. Leong. 1995. Natural killer enhancement factor-like gene
of rainbow trout (Oncorhynchus mykiss). Immunogenetics 42:438-439. Ore. Ag. Exper.
Station Tech. Paper No. 10,761.
Leong, J. and L. Bootland, et al. 1995. Viral vaccines in Aquaculture. J. Marine
Biotechnology 3:16-21. Ore. Ag. Exper. Station Tech. Paper No. 10,701.
Leong, J. 1995. Molecular biological tools to detect fish pathogens. J. Fish Biology 47
(Supplement A), 61-75. Ore. Ag. Exper. Station Tech. Paper No. 10,832.
Hsu, Y-L., Y.-H. Yang, Y.-C.Chen, M.-C. Tung, J.-L. Wu, M.H. Engelking, and J. C. Leong.
1995. Development of an in vitro subculture system for the oka organ (Lymphoid tissue) of
Penaeus monodon. Aquaculture 136:43-55.
Anderson, E., D. V. Mourich, and J. C. Leong. 1996. Gene expression in rainbow trout
(Oncorhynchus mykiss) following intramuscular injection of DNA. Molecular Marine Biology
and Biotechnology, 5(2):105-113. Oreg. Agricultural Expt. Station Tech. Paper No. 10,936.
Leong Pubs, p. 5
Anderson, E. D., D. V. Mourich, S. Fahrenkrug, S. LaPatra, J. Shepherd, and J. C. Leong.
1996. Genetic Immunization of rainbow trout (Oncorhynchus mykiss) against infectious
hematopoietic necrosis virus. Molecular Marine Biology and Biotechnology, 5(2):114-122.
Oreg. Agricultural Expt. Station Tech. Paper No. 10,937.
Perez, L., P. P. Chiou, and J. C. Leong. 1996. The Structural Proteins of Infectious
Pancreatic Necrosis Virus are not Glycosylated. J. Virology 70(10):7247-7249, 1996. Oreg.
Agricultural Expt. Station Tech. Paper No. 10,895.
Trobridge, G.D., P. P. Chiou, C. H. Kim, and J. C. Leong. 1997 Induction of the Mx protein of
rainbow trout Oncorhynchus mykiss in vitro and in vivo with poly IC and infectious
hematopoietic necrosis virus. Diseases of Aquatic Organisms 30:91-98.
Trobridge, G.D., P.P. Chiou, and J. C. Leong. 1997. Cloning of the rainbow trout
(Oncorhynchus mykiss) Mx2 and Mx3 cDNAs and characterization of trout Mx protein
expression in salmon cells. J. Virology 71:5304-5311.
J. C. Leong, E. Anderson, L.M. Bootland, P.-W. Chiou, M. Johnson, C. Kim, D. Mourich, and
G. Trobridge. 1997. Fish Vaccine Antigens Produced or Delivered by Recombinant DNA
Technologies. R. Gudding, A. Lillehaug, P.J. Midtlyng, F. Brown (eds.). Fish Vaccinology.
Dev. Biol. Stand. Basel, Karger 90: 267-277.
B. Robertsen, G. Trobridge, and J. C. Leong. 1997. Molecular cloning of double-stranded
RNA inducible Mx genes from Atlantic salmon (Salmo salar L.). Developmental &
Comparative Immunology 21 (5):397-412.
Trobridge, G.D., S. E. LaPatra, C. H. Kim, and J. C. Leong. 2000. Mx mRNA expression and
RFLP analysis of rainbow trout (Oncorhynchus mykiss) genetic crosses selected for
susceptibility or resistance to IHNV. Diseases of Aquatic Organisms 40:1-7. Ore. Ag.
Exper. Station Tech. Paper No. 11,214.
C. H. Kim, D. M. Dummer, P.P. Chiou, and J. C. Leong. 1998. Defective Interfering particle
mediated persistence of IHNV. Journal of Virology 73:843-849. Ore. Ag. Exper. Station
Tech. Paper No. 11,278.
J. C. Leong, G. D. Trobridge, C.H.Y. Kim, M. Johnson, and B. Simon. 1998. Interferoninducible Mx proteins in fish. Immunological Reviews 166:349-363.
Johnson, M. C., J. M. Maxwell, P. C. Loh, and J. C. Leong. 1999. Molecular characterization
of the glycoproteins from two warm water rhabdoviruses: snakehead rhabdovirus (SHRV)
and rhabdovirus of penaeid shrimp (RPS)/spring viremia of carp virus (SVCV). Virus
Research 64. 95-106.
Johnson, M.C., B. E. Simon, C.H. Kim, and J. C. Leong. 2000. Production of recombinant
snakehead rhabdovirus (SHRV): the NV protein is not required for viral replication.” J.
Virology 74:2343-2350.
Kim, C. H., M. C. Johnson, J. D. Drennan, B. E. Simon, E. Thomann, and J. C. Leong. 2000.
DNA Vaccines encoding viral glycoproteins induce non-specific immunity and Mx protein
synthesis in fish. J. Virology 74: 7048-7054.
Chiou, P. P., C. H. Kim, P. Ormonde, and J. C. Leong. 2000. The infectious hematopoietic
necrosis virus matrix protein inhibits host-directed gene expression and induces
morphological changes of apoptosis in cell cultures. J. Virology 74:7619-7627.
Leong Pubs, p. 6
Nygaard, R., S. Husgard, A-I. Sommer, J. C. Leong, and B. Robertsen. 2000. Induction of
Mx protein by interferon and double-stranded RNA in Salmonid cells. Fish & Shellfish
Immunology 10:435-450.
Simon, B., J. Nomellini, P. Chiou, W. Bingle, J. Thornton, J. Smit, and J. Leong. 2001.
Recombinant vaccines against infectious hematopoietic necrosis virus: production by the
Caulobacter crescentus S-layer protein secretion system and evaluation in laboratory trials.
Dis. Aquat. Org. 44:17-27.
T. L. Crippen, L. M. Bootland, J. C. Leong, M. S. Fitzpatrick, C. B. Schreck, and A. T. Vella.
Analysis of Salmonid Leukocytes Purified by Hypotonic Lysis of Erythrocytes. 2001. Journal
of Aquatic Animal Health 13:234-245.
Alonso, M. and J. C. Leong. 2002. Suppressive subtraction libraries to identify interferon-inducibe genes in fish.
Marine Biotechnology 4: 74-80.
Alonso, M., M. Johnson, B. Simon, and J. C. Leong. 2003. A specific expression vector containing the interferon
regulatory factor 1A (IRF1A) promoter for genetic immunization of fish. Vaccine 21: 1591-1600..
Milston, R.H., A.T. Vella, T. L. Crippen, M. S. Fitzpatrick, J. C. Leong, and C. B. Schreck. 2003.
In Vitro Detection of Functional Humoral Immunocompetence in Juvenile Chinook Salmon (Oncorhynchus
tshawytscha) using Flow Cytometry. Developmental and Comparative Immunology 15: 145-58.
Milston, R. H., M. S. Fitzpatrick, A. Vella, D. Undersen, G. Feist, S. Clements, T. Crippen, J. Leong, and C. B.
Schreck. 2003. Short term exposure of Chinook salmon (Oncorhynchus tshawytscha) to o, p’ DDE or DMSO during
early life history stages causes long-term humoral immunosuppression. Environ. Health Perspectives 111, 16011608.
Leisy, D., T.D. Lewis, J. C. Leong, and G. Rohrmann. 2003. Transduction of cultured fish cells
with recombinant baculoviruses. J. General Virology 84: 1173-1178.
M. Alonso, C. H. Kim, M. C. Johnson, M. Pressley, and J.C. Leong. 2004. The NV gene of
snakehead rhabdovirus (SHRV) is not required for pathogenesis and a heterologous glycoprotein
can be incorporated into the SHRV envelope. J. Virology 78: 5875-5882.
I. Misumi, A.T. Vella, J.C. Leong, T. Nakanishi, and C.B. Schreck. 2005. p,p’-DDE depresses
the immune competence of Chinook salmon (Oncorhynchus tshawytscha) leucocytes. Fish and
Shellfish Immunology 19:97-114.
Hollingsworth, L.L., R.A. Kinzie III, D. A. Krupp, T. D. Lewis, and J. C. Leong. 2005. Phototaxis of
motile zooxanthellae to green light may facilitate symbiont capture by coral larvae. Coral Reefs
24(4):523.
Alonso, M., D.A. Stein, E. Thomann, H. M. Moulton, J. C. Leong, P. Iversen, and D. V. Mourich.
2005. Inhibition of infectious haematopoietic necrosis virus in cell cultures with peptide
conjugated morpholino oligomers. J. Fish Diseases 28 (7):399-410.
Hagedorn, M., V.L. carter, R.A. Steyn, D. Krupp, J. C. Leong, R.P. Lang, and T. R.l Tiersch.
2006. Preliminary studies of sperm cryopreservation in the mushroom coral, Fungia scutaria.
Cryobiology 52(3):454-8.
Hagedorn, M., R. Pan, E. F. Cox, L. L. Hollingsworth, D. A. Krupp, T. D. Lewis, J. C. Leong, P.
Mazur, W. Rall, D. MacFarlane, G. Fahy, and F. W. Kleinhaus. 2006. Coral larvae conservation
physiology and reproduction. Cryobiology 52 (1):33-47.
Robertsen, B., J. Zou, C. Secombes, J. Leong. 2006. Molecular and expression analysis of an
interferon-gamma inducible guanylate-binding protein from rainbow trout. Developmental &
Comparative Immunology 30(11): 1023-1033.
Leong Pubs, p. 7
Misumi, I., T. Yada, J. C. Leong, and C. B. Schreck. (2009) The effect of in vitro exposure to
tributylin on the immune competence of Chinook salmon (Oncorhynchus tshawytscha)
leukocytes. Archives of Environmental Contamination and Toxicology 56 (2): 229-237.
Palumbi, S.R., J. D. Allen, M. W. Beck, D. G. Fautin, M.J. Fogarty, B. S. Halpern, L.S. Incze, J.
Leong, E. Norse, J. J. Stachowicz, D. H. Wall, and P. A. Sandifer. 2009. Managing for Ocean
Biodiversity: Creating a National Biodiversity Agenda to Sustain Marine Ecosystem Services.
Frontiers in Ecology and the Environment 7(4):204-211..
Van Oppen, Madeleine JH, Jo-Ann Leong, Ruth D. Gates. 2009. Coral-virus interactions: a
double-edged sword? Symbiosis 47 (1):1-8.
.
Leong J C. 2009. Fish Viruses. Encyclopedia of Virology, 5 vols. (B.W.J. Mahy and M.H.V. Van
Regenmortel, Editors), pp. 227-234 Oxford: Elsevier.
Leong, J. C., and G. Kurath. 2009. Novirhabdoviruses. The Springer Index of Viruses. (C.A.
Tidona and G. Darai, Editors), online. Springer-Verlag.
Work, T.M., J. Dagenais, G.H. Balazs, J. Schumacher, T. d. Lewis, J. C. Leong, R. N. Casey, and
J. W. Casey. 2009. In vitro biology of fibropapilloma-associated turtle herpesvirus and host cells
in Hawaiian green turtles (Chelonia mydas). Journal of General Virology 90:1943-1950.
Ackermann, M., M. Koriabine, P. J. De Jong, T. D. Lewis, U. Buchler, T. M. Work, J. Degenais,
G. H. Balazs, and J. C. Leong. 2008. Detection of a gene encoding sialylytransferase in a BAC
comprising the genome of the fibropapilloma-associate turtle herpesvirus. Virology, in review.
M. Alonso, E. Thomann, I. Misumi, T. Vella, C. Schreck, and J. C. Leong. 2008. Fish Mx protein:
a potential environmental biomarker. Fish and Shellfish Immunology, in preparation.
Lewis, T.D., E. F. Cox, and J. C. Leong. 2007. A method for cellular disaggregation and cell-type
isolation in scleractinian corals. Marine Biotechnology, in preparation.
b. Reports, Theses, Proceedings
Leong, J. 1971. Studies of the synthesis of Rous sarcoma virus in synchronized cells and
Identification of intracellular viral RNA. Doctoral thesis, University of California San Francisco
Medical Center, San Francisco, California.
Leong, J. and S. Turner. 1979. Isolation of water-borne infectious hematopoietic necrosis
virus. Fish Health News 8(2):vi-vii.
Leong, J. and J. L. Fryer. 1980. Microbiological assessment of river water contamination by
fish hatchery effluent. Office of Water Research and Technology Publication. WRRI 66. 43
pp.
Hedrick, R. P., J. C. Leong, and J. L. Fryer. 1981. Persistent infection of salmonid cells with
infectious pancreatic necrosis virus (IPNV): A model for the regulatory and management
aspects. Proceedings from a symposium sponsored by the U. S. Food and Drug
Administration. February 12-14,1980. New Orleans, Louisiana.
Hedrick, R. P., J. C. Leong, and J. L. Fryer. 1981. Establishment and maintenance of the
carrier state in salmonids with infectious pancreatic necrosis virus. In: Sixth FDA Science
Symposium on Aquaculture (Constatine Zervos, editor), Human Services publication.
Leong Pubs, p. 8
Leong, J. C., Y. L. Hsu, H. M. Engelking, J. L. Fendrick, L. K. Durrin, and G. Kurath. 1983.
Methodology for diagnosing IHNV infection in Fish. In: Proceedings of a Workshop on Viral
Diseases of Salmonid Fishes in the Columbia River Basin. October 7-8, 1982. Portland,
Oregon. pp. 23-50.
Leong, J. C. and T. Y. Barila (eds.). 1983. Proceedings of a Workshop on Viral Diseases of
Salmonid Fishes in the Columbia River Basin. October 7-8, 1982. Portland, Oregon.
Special Publication, Bonneville Power Administration, Portland, Oregon. 173 pp.
Leong, J. 1985. Rapid diagnosis of IHN virus infection in salmon and steelhead trout. Final
Report, Bonneville Power Administration. 145 pp.
Leong, J. 1986. Evaluation of a subunit vaccine to infectious hematopoietic necrosis (IHN)
virus. Annual Report, Bonneville Power Administration. 55 pp.
Leong, J. C., R. Barrie, H. M. Engelking, R. Gilmore, M. T. F. Huang, Y. L. Hsu, J. F. Koener,
G. Kurath, D. S. Manning, L. Oberg, and J. Wirkkula. 1988. Vaccines for viral diseases of
fish. Proceedings of Aquaculture International Congress, Vancouver, B.C., Canada,
September 6-9. pp. 471-478.
Tengelsen, L., E. Anderson, and J. C. Leong. 1989. Variation in fish interferon-like activity:
cell-line production and IHNV virus isolate sensitivity. Fish Health News 17(2):4.
Leong, J. C. 1989. Evaluation of a subunit vaccine to infectious hematopoietic necrosis
(IHN) virus. Annual Report, Bonneville Power Administration. 55 pp.
Leong, J. C., R. Barrie, H. M. Engelking, J. F.-Koener, R. Gilmore, J. Harry, G. Kurath, D. S.
Manning, C. L. Mason, L. Oberg, J. Wirkkula. 1990. Recombinant viral vaccines in
aquaculture - a progress report. Proceedings of 16th USA/Japan Meeting of Natural
Resources Panel on Aquaculture (R. Svrjcek, Ed.), Charleston, South Carolina, October
1987. NOAA Technical Report NMFS 92:27-31. Ore. Ag. Exper. Station Tech. Paper No.
8961.
Ristow, S. S., J. M. Arnzen, and J. C. Leong. 1990. Research studies on the life cycle of
infectious hematopoietic necrosis virus. Annual Report, Bonneville Power Administration. 55
pp.
Engelking, H. M., J. C. Leong, J. Follett, E. Emmenegger, and T. Meyers. 1991. Subunit
vaccine to IHNV provides protection in sockeye salmon fry in Alaska. FHS/AFS Newsletter
19(2):4-5.
Anderson, E., C. Cary, J. S. Rohovec, and J. C. Leong. 1991. Detection of infectious
hematopoietic necrosis virus mRNA by in situ hybridization. Proceedings of the Second
International Symposium on Viruses of Lower Vertebrates, Corvallis, Oregon, July 29-31, pp.
9-16. Ore. Ag. Exper. Station Tech. Paper. No. 9685.
Chen, L., S. Ristow, and J. C. Leong. 1991. Epitope mapping and characterization of the
infectious hematopoietic necrosis virus nucleoprotein. Proceedings of the Second
International Symposium on Viruses of Lower Vertebrates, Corvallis, Oregon, July 29-31, pp.
65-72. Ore. Ag. Exper. Station Tech. Paper. No. 9689.
Mason, C. L., R. Duncan, P. Dobos, and J. C. Leong. 1991. Sequence analysis and
bacterial expression of infectious pancreatic necrosis virus (IPNV) genome segment B and its
encoded VP1 protein: a putative RNA-dependent RNA polymerase. Proceedings of the
Second International Symposium on Viruses of Lower Vertebrates, Corvallis, Oregon, July
29-31, pp. 25-32. Ore. Ag. Exper. Station Tech. Paper. No. 9684.
Leong Pubs, p. 9
Mourich, D. V. and J. C. Leong. 1991. Mapping of the immunogenic regions of the IHNV
glycoprotein in rainbow trout and mice. Proceedings of the Second International Symposium
on Viruses of Lower Vertebrates, Corvallis, Oregon, July 29-31, pp. 93-100. Ore. Ag. Exper.
Station Tech. Paper. No. 9688.
Tengelsen, L. A., G. D. Trobridge, and J. C. Leong. 1991. Characterization of an inducible
interferon-like antiviral activity in salmonids. Proceedings of the Second International
Symposium on Viruses of Lower Vertebrates, Corvallis, Oregon, July 29-31, pp. 219-226.
Ore. Ag. Exper. Station Tech. Paper. No. 9690.
Leong, J. C., E. D. Anderson, L. Bootland, B. Drolet, L. Chen, C. L. Mason, D. V. Mourich,
and G. Trobridge. 1991. Biotechnological approaches to development of salmonid fish
vaccines. Proceedings of Oji International Symposium on Salmonid Diseases, Sapporo,
Japan, October 22-24, pp. 250-258. Ore. Ag. Exper. Station Tech. Paper No. 9740.
Thorgaard, G. and Leong, J. C. 1992. Biotechnology in Aquaculture. In: Aquaculture
Research Needs for 2000 A.D., Proceedings of U.S.D.A. International Symposium on
Aquaculture, November 14-18, 1991. Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi,
India. pp. 205-207.
Barrie, R. J., C. L. Mason, and J. C. Leong. 1992. Identification of a conserved antigenic
domain in the major capsid protein of infectious pancreatic necrosis virus. Proceedings of
19th USA/Japan Meeting of Natural Resources Panel on Aquaculture (R. Svrjcek, Ed.), Ise,
Japan, October 24-November 1, 1990. NOAA Technical Report NMFS 111:15-20. Ore. Ag.
Exper. Station Tech. Paper No. 9327.
Leong, J. C., E. D. Anderson, L. Bootland, B. Drolet, L. Chen, C. L. Mason, D. Mourich, and
G. Trobridge. 1993. Biotechnologic advances in fish disease research. Proceedings,
International Marine Biotechnology Conference, Society of Industrial Microbiology, Baltimore,
Maryland, October 13-16. Wm. Brown Communications, Iowa, pp. 573-586. Ore. Ag. Exper.
Station Tech. Paper No. 9729.
Leong, J.C., E. Anderson, L. Bootland, L. Chen, P.-W. Chiou, B. Drolet, H.M. Engelking, C.
Mason, D. Mourich, G. Trobridge, and M. Wilson. 1993. Molecular and Biotechnological
Approaches to the Control of Viral Diseases In Fish. Proceedings of the Third Pacific Rim
Biotechnology Conference, Taipei, Taiwan, Republic of China, August 19-21, 1992, pp. 220223.
Leong, J. C., E. Anderson, P. W. Chiou, B. Drolet, C. Kim, D. Mourich, P. Ormonde, L. Perez,
and G. Trobridge. 1995. Molecular approaches to control viral diseases in fish.
Proceedings, International Symposium in Biotechnology Applications in Aquaculture. Taipei,
Taiwan, Republic of China, Dec. 10-15, 1994, pp. 113-124. Ore. Ag. Exper. Station Tech.
Paper No. 10,832.
Hsu, Y.L., Y.-H. Yang, Y.C. Chen, M.-C. Tung, J.-L. Wu, H.M. Engelking, and J. C. Leong.
1995. Development of an In Vitro Subculture System for Prawn Tissues. Proceedings,
International Symposium in Biotechnology Applications in Aquaculture. Taipei, Taiwan,
Republic of China, Dec. 10-15, 1994, pp. 161-170.
Leong, J. C., E. Anderson, L.M. Bootland, P-W. Chiou, M. Johnson, C. Kim, D. Mourich, and
G. Trobridge. 1996. Fish Vaccine Antigens produced or delivered by recombinant DNA
technologies. Proceedings of Symposium on Fish Vaccinology in Oslo, Norway. June 5-7,
1996.
Leong Pubs, p. 10
Leong, J. C., E. Anderson, L.M. Bootland, P.W.Chiou, M. Johnson, C. Kim, D. Mourich, and
G Trobridge. 1997. Cytokines and Vaccines for Aquaculture. In Proceedings of Workshop
on New approaches to Viral Diseases of Aquatic Animals. ed. Y. Inui and J. Winton in Kyoto,
Japan. January 21-24, 1997.
Leong, J. C., T. Crippen, J. Drennan, M. Johnson, D. Jordan, C. Kim, B. Simon, and E.
Thomanne. 2000. Development of DNA Vaccines for Fish. Suisanzoshoku 48(2):285-290,
2000. A New Paradigm for Aquaculture 3. Proceedings of the International Symposium on
the New Paradigm for Aquaculture. Feb. 8-10, 2000. Nippon Convention Center, Chiba,
Japan. Edited by H. Sugita, R. Ueda, K. Yoshihara, and N. Taniguchi. Japan Aquaculture
Society.
Hollingsworth, L. L., T. D. Lewis, and J. C. Leong. 2005. Early onset and expression of
fluorescent proteins in the larvae of the mushroom coral, Fungia scutaria Lamarck 1801.
Proceedings of the 10th International Coral Reef Symposium, Okinawa, Japan, June 28-July 1,
2004, published 2006; pp. 99-105.
Leong, J.C., and M.A. Rivera. 2006. Quarterly Progress Report III. January-March 2006.
Hawaii Institute of Marine Biology – Northwestern Hawaii Islands Coral Reef Research
Partnership. 44 pp.
Leong, J. C., M.A. Rivera, and J. Barrett. 2006. Quarterly Progress Report IV. April-June
2006. Hawaii Institute of Marine Biology – Northwestern Hawaiian Islands Coral Reef
Research Partnership. 34 pp.
Leong, J.C., and C. Wiener. 2007. Semi-annual progress report VII. November 2007.
Hawai‘i Institute of Marine biology –Northwestern Hawaiian Islands Coral Reef Research
Partnership,
c. Books and Chapters
Levy, J., P. Volberding, H. Oppermann, and J. Leong. 1982. Retroviruses and
differentiation. Pages 451-469. In: R. F. Revotella et al., eds. Symposium on "Expression
of differentiated functions in cancer cells." Ravens Press, New York.
Leong, J. C., Y. L. Hsu, and H. M. Engelking. 1983. Synthesis of the structural proteins of
infectious hematopoietic necrosis virus. Pages 61-71. In: J. Crosa, ed. Molecular
Approaches to the Study of Bacterial and Viral Diseases of Fish. Seattle, Washington.
Leong, J., J. L. Fryer, and J. R. Winton. 1988. Vaccines for infectious hematopoietic
necrosis virus. Pages 193-203. In: A. E. Ellis, ed. Fish Vaccination. Academic Press,
London. Ore. Ag. Exper. Station Tech. Paper No. 8249.
Leong, J. C. and J. L. Fryer. 1993. Viral Vaccines for Aquaculture. Pages 225-240. In:
Annual Review of Fish Diseases, Vol. 3. Pergamon Press, N.Y.
Wilkinson, D., D. Mager, and J. C. Leong. 1994. Human Endogenous Retroviruses. Pages
465-535. In: J. Levy, ed. The Retroviruses, Comprehensive Virology, Vol. 3. Plenum Press
Bootland, L.M. and J. C Leong. 1999. Infectious Hematopoietic Necrosis Virus (IHNV). In:
P. Woo, ed., Viral, Bacterial and Fungal Infections, Vol. II. Ed. P.T. K. Woo and D. W. Bruno.
CAB International Publishing, pp.57-121.
J. C. Leong and C. L. Mason. 1998. Infectious pancreatic necrosis virus endopeptidase. In:
A. Bartlett, N.D. Rawlings, and J. F. Woessner, eds. Handbook of Proteolytic Enzymes. pp.
1593-96.
Leong Pubs, p. 11
E. D. Anderson and J. C. Leong. 2000. Development of DNA Vaccines for Salmonid Fish.
In DNA Vaccines: Methods and Protocols. ed. D. Lowrie and R. Whalen. Humana Press.
London, U.K., pp.105-121.
J.C. Leong, D. Brown, P. Dobos, F.S.B. Kibenge, J. E. Ludert, H. Muller, E. Mundt, and B.
Nichsolson. Birnaviridae. 2000. In the 7th Report of the International Committee on the
Taxonomy of Viruses.
A. Benmansour, C.H. Calisher, D. Dietzgen, R.-X. Fang, A.O. Jackson, G. Kurath, J. C.
Leong, S. Nadin-Davies, R.B. Tesh, N. Tordo, and P. J. Walker. 2000. Rhabdoviridae. In the
7th Report of the International Committee on the Taxonomy of Viruses.
C. Kim and J. C. Leong. Fish Viruses. 1999 In Encyclopedia of Virology, Second Edition.
ed. R. G. Webster and A. Granoff. Academic Press, London, U.K, pp. 558-568.
B.E. Simon and J. C. Leong. 2003. A Review of Recombinant Vaccines for Aquaculture:
Bacterial Systems for Vaccine Production and Delivery. Chapter 16 in Agrobiotechnology
and Plant Tissue Culture. Ed. Sant S. Bhojwani and Woong-Young Soh, Oxford & IBH
Publishing Co., New Delhi, India.
Leong, J.C., and G. Kurath. 2009. Infectious Hematopoietic Necrosis Virus. In
J. C. Leong. 2003. DNA Vaccination of fish: transient expression and protection against
pathogens. Aquaculture: Retrsopective and Outlook. Proceedings of the Meeting, AIP
Aquaculture Summit. November 4-7, 2002. Honolulu, Hawaii
T. D. Lewis and J-A. C. Leong. 2004. Viruses of Fish. Chapter 2 in Current Trends in the
Study of Bacterial and Viral Fish and Shrimp Diseases. Molecular Aspects of Fish & Marine
Biology, Vol. 3, pp. 39-81. ed. K. Y Leung. World Scientific Publishing Co., Singapore.
J. C. Leong. 2007. Fish Viruses. In Encylopedia of Virology. Ed. Brian J. Mahy and Marc
HV von Regenmortel. Published by Elsevier online at http:// ScienceDirect.com.
Bootland, L.M. and J. C Leong. 2009. Infectious Hematopoietic Necrosis Virus (IHNV). In:
P. Woo, ed., Viral, Bacterial and Fungal Infections, Vol. II. Ed. P.T. K. Woo and D. W. Bruno.
CABI International Publishing, in press.
d. Reviews and Abstracts
Bishop, J. M., H. Varmus, W. Levinson, A. Faras, A. Garapin, J. Leong, J. McDonnell, and J.
Taylor. 1971. RNA-directed DNA polymerase and the replication of Rous sarcoma virus.
Abstract, Fed. Proc. 30(3):1050.
Smith, H. S., S. Turner, J. Leong, and P. Rigby. 1975. Tumor Virus Meeting. Effect of
culture conditions on an SV40-transformed clone of Balb/3T3. Cold Spring Harbor
Laboratory, New York.
Leong, J. and J. Levy. 1976. Further characterization of a factor in mouse serum that
specifically neutralizes murine xenotropic C-type virus. Abstract, Fed. Proc. 35(3):391.
Leong, J., J. Nelson, and J. Levy. 1978. Reverse transcriptase-like activity in extracts of
human placentas. Annual Meeting of American Society for Microbiology.
Leong Pubs, p. 12
Hedrick, R. P., J. C. Leong, and J. L. Fryer. 1978. Persistent infection with infectious
pancreatic necrosis virus (IPNV) in piscine cells. Abstracts of the Annual Meeting, American
Society for Microbiology S 334, 267.
Fendrick, J. and J. Leong. 1980. Growth of IHNV in mosquito and fish cell lines. Northwest
Branch, American Society for Microbiology.
Nelson, J., D. Libby, and J. Leong. 1980. A new polymerase DNA activity in human
placental tissue. Northwest Branch, American Society for Microbiology.
Engelking, H. M., and J. Leong. 1980. IHNV persistently infects chinook salmon embryo
cells. Northwest Branch, American Society for Microbiology.
Fendrick, J. L., W. J. Groberg, Jr., and J. C. Leong. 1980. Sensitivity of five piscine cell lines
for isolation of IHN virus from naturally infected salmonid fishes. American Fisheries Society,
Fish Health Section, 4th Biennial Conference, Seattle, Washington.
Leong, J., Y. L. Hsu, A. W. Roberts, and H. M. Engelking. 1981. Strains of infectious
hematopoietic necrosis (IHN) virus may be identified by structural protein differences.
International Symposium on Fish Biologics: Serodiagnostics and Vaccines, Leetown, West
Virginia.
Kurath, G. and J. C. Leong. 1981. Transcription of infectious hematopoietic necrosis virus in
vitro. Pacific Slope Biochemical Conference, Davis, California.
Wong, S. W. and J. C. Leong. 1981. A large beta-like DNA polymerase in human
trophoblast tissue. Pacific Slope Biomedical Conference, Davis, California.
Hsu, Y. L., H. M. Engelking, and J. C. Leong. 1981. Viral protein synthesis in infectious
hematopoietic necrosis virus (IHNV)-infected salmon cells. Pacific Slope Biomedical
Conference, Davis, California.
Leong, J. C., Y. L. Hsu, and H. M. Engelking. 1982. Synthesis of the structural proteins of
infectious hematopoietic necrosis virus. Northwest Branch, American Society for
Microbiology.
Leong, J. C., Y. L. Hsu, and H. M. Engelking. 1983. Strain typing of IHN virus in the
Columbia River Basin. American Fisheries Society Symposium, Oregon Chapter. Corvallis,
Oregon.
Leong, J. C., S. D. Wood, A. O. Lyford, and J. A. Levy. 1983. Purification of a specific
reverse transcriptase inhibitor from human placenta. American Association for Cancer
Research. San Diego, California
Huang, M., E. B. Stephens, and J. C. Leong. 1984. Molecular cloning of infectious
pancreatic necrosis virus. Oregon American Fisheries Society Meeting, Kahneeta, Oregon.
Engelking, H. M., Y. L. Hsu, G. Kurath, and J. C. Leong. 1984. Rapid diagnosis of IHN virus.
Oregon American Fisheries Society Meeting, Kahneeta, Oregon.
Stephens, E. B., A. Hoffman, J. A. Levy, and J. C. Leong. 1984. Isolation of a retrovirus from
mink cells inoculated with placental extracts. American Association for Cancer Research.
Abstract of Toronto meeting 25:390.
Leong Pubs, p. 13
Feyereisen, J. K., E. B. Stephens, T. Kimura, and J. C. Leong. 1984. The physical mapping
of fish herpesvirus DNA. American Association for Cancer Research. Abstract of Toronto
meeting. 25:387.
Kurath, G., and J. C. Leong. 1984. The physical mapping of the IHN genome. Sixth
International Congress of Virology. Sendai, Japan.
Hsu, Y. L. and J. C. Leong. 1984. Molecular comparison of infectious hematopoietic
necrosis virus strains. Sixth International Congress of Virology. Sendai, Japan.
Huang, M., E. Stephens, and J. Leong. 1985. The molecular cloning of infectious pancreatic
necrosis virus. The Second International Conference of Warm Water Aquaculture-Finfish.
Laie, Hawaii. February 5-8.
Leong, J. C., J. F. Koener, R. Gilmore, and G. Kurath. 1985. Molecular characterization of a
fish rhabdovirus, IHNV. American Society for Virology Annual Meeting, Albuquerque, New
Mexico. July 21-25.
Leong, J. C., H. M. Engelking, J. F. Koener, R. Gilmore, G. Kurath, and C. Passavant. 1985.
Immunization of salmonid fishes to infectious hematopoietic necrosis virus (IHNV).
Bonneville Power Administration Annual Review, March.
Leong, J. C., R. Gilmore, J. Feyereisen-Koener, G. Kurath, H. M. Engelking, and C.
Passavant. 1987. Development of a vaccine to IHN virus by molecular cloning. Abstract, VII
International Congress of Virology. Edmonton, Canada. August 9-14.
Manning, D. S. and J. C. Leong. 1988. Deletion mapping and expression of the large
genomic segment of infectious pancreatic necrosis virus. Abstract, 7th Annual Meeting,
American Society for Virology, University of Texas, Austin, Texas. June 12-16.
Engelking, H. M., J. B. Harry, and J. C. Leong. 1988. Properties of a temperature sensitive
isolate of infectious hematopoietic necrosis virus, a rhabdovirus of salmonid fish. 7th Annual
Meeting, American Society for Virology, University of Texas, Austin, Texas. June 12-16.
Barrie, R., D. S. Manning, and J. C. Leong. 1988. Characterization of the epitopes of the
major capsid protein of infectious pancreatic necrosis virus. 7th Annual Meeting, American
Society for Virology, University of Texas, Austin, Texas. June 12-16.
Leong, J. C., R. Barrie, H. M. Engelking, J. Feyereisen-Koener, R. D. Gilmore, M. T. F.
Huang, Manning, D. S. and C. Mason. Development of viral vaccines for fish by molecular
cloning. 1988. Abstract, International Conference on Fish Health, Fish Health Section,
American Fisheries Society. Vancouver, Canada. July 17-21.
Leong, J. C., R. Barrie, H. M. Engelking, R. Gilmore, M. T. F. Huang, Y. L. Hsu, J. F. Koener,
G. Kurath, D. S. Manning, L. Oberg, and J. Wirkkula. 1988. Vaccines for viral diseases of
fish. Aquaculture International Congress. Vancouver, B.C., Canada. September 6-9.
Leong, J. C., R. Barrie, H. M. Engelking, J. Feyereisen-Koener, R. D. Gilmore, M. T. F.
Huang, G. Kurath, D. S. Manning, and L. Tengelsen. 1988. Recombinant vaccines in
aquaculture. Abstract, First Annual International Symposium on Marine Biology, University of
Maryland. Baltimore, Maryland. October 9-11.
Leong, J. C. 1988. Biotechnology in Aquaculture. Abstract, Conference on Aquaculture in
the Year 2000, Office of International Cooperation and Development, FERRO. New Delhi,
India. November 14-18.
Leong Pubs, p. 14
Mason, C. L., R. Barrie, D. S. Manning, and J. C. Leong. 1989. Nucleotide and amino acid
sequence analysis of antigenic determinants of infectious pancreatic necrosis virus Sp.
Annual meeting and symposium, American Fisheries Society. Bend, Oregon. February 8-11.
Mourich, D. V., L. Xu, J. Wirkkula, J. S. Rohovec, H. M. Engelking, and J. C. Leong. 1989.
Bacterial expression of the IHNV glycoprotein gene: Deletion of a neutralizing epitope with
monoclonal antibodies. Annual meeting and symposium, American Fisheries Society. Bend,
Oregon. February 8-11.
Leong, J. C. 1989. Bacterial expression of a rhabdovirus glycoprotein for vaccination of fish
against infectious hematopoietic necrosis virus. Abstract, Annual Meeting, American Society
for Microbiology, New Orleans, Louisiana. May 15-19.
Leong, J. C., R. Barrie, C. L. Mason, P. Caswell-Reno, and B. Nicholson. 1989.
Characterization of immunoreactive regions of the major capsid protein for infectious
pancreatic necrosis virus. Abstract, VII Intl. Congress of Virology. Banff, Canada. October
15-21, 1989.
Leong, J., L. Xu, S. J. Chen, D. V. Mourich, L. Oberg, and S. Ristow. 1990. Immunogenic
domains of the G and N proteins of infectious hematopoietic necrosis virus. Abstract, VIII
International Congress of Virology. Berlin, East Germany. August 26-31.
Engelking, H. M. and J. C. Leong. 1991. Subunit vaccine trials using glycoprotein and
nucleoprotein constructs of the rhabdovirus, infectious hematopoietic necrosis virus.
Abstract, Tenth Annual Meeting of American Society for Virology, Ft. Collins, Colorado. July
7-11.
Leong, J.C. 1991. A cloned vaccine for infectious hematopoietic necrosis virus in fish.
Abstract, Annual meeting of Canadian Bacterial Diseases Network. Vancouver, B.C.,
Canada. July 7-10.
Anderson, E. D. and J. C. Leong. 1991. Detection of infectious hematopoietic necrosis virus
mRNA using in situ hybridization. Abstract, Second International Symposium on Viruses of
Lower Vertebrates. Oregon State University, Corvallis, Oregon. July 29-31.
Chen, L., S. Ristow and J. C. Leong. 1991. Epitope mapping and characterization of the
infectious hematopoietic necrosis virus nucleoprotein. Abstract, Second International
Symposium on Viruses of Lower Vertebrates. Oregon State University, Corvallis, Oregon.
July 29-31.
Mason, C. L., R. Duncan, P. Dobos and J. C. Leong. 1991. Sequence analysis and bacterial
expression of infectious pancreatic necrosis virus (IPNV) genome segment B and its encoded
VP1 protein: a putative RNA-dependent RNA polymerase. Abstract, Second International
Symposium on Viruses of Lower Vertebrates. Oregon State University, Corvallis, Oregon.
July 29-31.
Mourich, D. V. and J. C. Leong. 1991. Mapping of the immunogenic regions of the IHNV
glycoprotein in rainbow trout and mice. Abstract, Second International Symposium on
Viruses of Lower Vertebrates. Oregon State University, Corvallis, Oregon. July 29-31.
Trobridge, G., L. A. Tengelsen and J. C. Leong. 1991. The search for a fish interferon gene.
Abstract, Second International Symposium on Viruses of Lower Vertebrates. Oregon State
University, Corvallis, Oregon. July 29-31.
Bootland, L. M. and J. C. Leong. 1991. Staphylococcal coagglutination: a rapid, simple
method of denitrifying infectious hematopoietic necrosis virus. Abstract, Fourteenth Annual
Leong Pubs, p. 15
American Fisheries Society-Fish Health Section Meeting/Thirty-second Annual Western Fish
Disease Conference, Newport, Oregon. August 1-3.
Mourich, D. V. and J. C. Leong. 1991. Effects of immune adjuvants on vaccination against
IHNV. Abstract, Fourteenth Annual American Fisheries Society/Fish Health Section
Meeting/Thirty-second Annual Western Fish Disease Conference, Newport, Oregon. August
1-3.
Drolet, B. S., J. C. Leong and J. Rohovec. 1991. Detection of infectious hematopoietic
necrosis virus nucleocapsid protein in infected fish tissues by alkaline phosphatase.
Abstract, Fourteenth Annual American Fisheries Society/Fish Health Section Meeting/Thirtysecond Annual Western Fish Disease Conference, Newport, Oregon. August 1-3.
Engelking, H. M. and J. C. Leong. 1991. Subunit vaccine trials using glycoprotein and
nucleoprotein constructs of the rhabdovirus, infectious hematopoietic necrosis virus.
Abstract, Fourteenth Annual American Fisheries Society/Fish Health Section Meeting/Thirtysecond Annual Western Fish Disease Conference, Newport, Oregon. August 1-3.
Wirkkula, J. R., H. V. Lorz, J. C. Leong and J. S. Rohovec. 1991. Examination for the latent
state of infectious hematopoietic necrosis virus in artificially infected rainbow trout. Abstract,
Fourteenth Annual American Fisheries Society/Fish Health Section Meeting/Thirty-second
Annual Western Fish Disease Conference, Newport, Oregon. August 1-3.
Leong, J. C., E. D. Anderson, L. Bootland, B. Drolet, L. Chen, C. L. Mason, D. Mourich, and
G. Trobridge. 1991. Biotechnologic advances in fish disease research. Abstract,
International Marine Biotechnology Conference, Society of Industrial Microbiology. Baltimore,
Maryland. October 13-16.
Mason, C. L. and J. C. Leong. 1991. Molecular characterization of the protease and RNAdependent RNA polymerase of infectious pancreatic necrosis, an aquatic birnavirus.
Abstract, International Marine Biotechnology Conference, Society of Industrial Microbiology.
Baltimore, Maryland. October 13-16.
Anderson, E. D. and J. C. Leong. 1991. Reduction of IHNV induced CPE by transgene
expression of the nucleocapsid gene. Abstract, International Marine Biotechnology
Conference, Society of Industrial Microbiology. Baltimore, Maryland. October 13-16.
Leong, J. C., E. D. Anderson, L. Bootland, B. Drolet, L. Chen, C. L. Mason, D. V. Mourich,
and G. Trobridge. 1991. Biotechnological approaches to development of salmonid fish
vaccines. Abstract, Oji International Symposium on Salmonid Diseases. Sapporo, Japan.
October 22-24.
Leong, J. C. 1992. Vaccines for viral diseases of fish. Session on Molecular Genetics and
Biotechnology, Aquaculture '92, World Aquaculture Society/American Fisheries Society.
Orlando, Florida. May 21-25.
Bootland, L. M., B. S. Drolet, H. V. Lorz, J. S. Rohovec, J. L. Fryer, and J. C. Leong. 1992.
Experimental infection of young salmonid fish with infectious hematopoietic necrosis virus
(IHNV). Abstract, 9th Annual Meeting of the Aquaculture Association of Canada, Vancouver,
B. C. May 31-June 4.
Drolet, B. S., J. S. Rohovec, and J. C. Leong. 1992. An immunohistochemical study of the
disease process of IHNV. Workshop, Eastern Fish Health/Fish Health Section, American
Fisheries Society. Auburn, Alabama. June 16-19.
Leong Pubs, p. 16
Drolet, B. S., J. C. Leong, and J. S. Rohovec. 1992. Detection of infectious hematopoietic
necrosis virus nucleocapsid protein in infected fish tissues by alkaline phosphatase.
Abstract, 11th Annual Meeting of American Society for Virology, Cornell University, Ithaca,
New York. July 11-15.
Leong, J. C., E. Anderson, L. Bootland, B. Drolet, L. Chen, P.-W. Chiou, C. L. Mason, D.
Mourich, G. Trobridge, and M. Wilson. 1992. Molecular and biotechnological approaches in
the control of viral diseases of fish. Abstract, 3rd Pacific Rim Biotechnology Conference,
Academia Sinica, Taipei, Taiwan, Republic of China. August 19-21.
Leong, J. C., E. Anderson, L. Bootland, L. Chen, P. W. Chiou, B. Drolet, C. L. Mason, D. V.
Mourich, P. Ormonde, G. Trobridge, M,. S. Wilson, and S. E. LaPatra. 1992. Recombinant
DNA vaccines for viral diseases of fish. Abstract, The Biotechnological Approaches to the
Culture and Diseases of Fish and Shellfish Workshop. University College, Cork, Ireland.
September 14-17.
Anderson, E. and J. C. Leong. 1993. Reduction of IHNV induced cytotoxicity by transgenic
expression of the nucleocapsid gene of IHNV. Abstract, 12th Annual Meeting, American
Society for Virology, Davis, California. July 10-14, 1993.
Chiou, P.-W. and J. C. Leong. 1993. The gene sequence and expression of the nonvirion
gene of Infectious Hematopoietic Necrosis Virus. Abstract, 12th Annual Meeting, American
Society for Virology, Davis, California. July 10-14, 1993.
Ormonde, P. and J. C. Leong. 1993. Sequence analysis of the matrix protein, M1, of
Infectious Hematopoietic Necrosis Virus. Abstract, 12th Annual Meeting, American Society
for Virology, Davis, California. July 10-14, 1993.
Trobridge, G. D., and J. C. Leong. 1994. Identification and characterization of the antiviral
Mx genes of rainbow trout. Abstract, International Symposium on the Diseases of Aquatic
Organisms, Seattle, Washington. Septenber 4-8, 1994.
Kim, C. H., J. R. Winton, and J. C. Leong. 1994. Characterization of neutralization-resistant
IHNV variants. Abstract, International Symposium on the Diseases of Aquatic Organisms,
Seattle, Washington. September 4-8, 1994.
Leong, et al. 1994. Viral vaccines for aquaculture. Abstract, International Meeting on Marine
Biotechnology, Tromso, Norway. August 4-8, 1994.
Piganelli, J. D., D. V. Mourich, J. C. Leong, and S. L. Kaattari. 1994. Assessment of
immunological memory to a T-dependent antigen in rainbow trout (Oncorhynchus mykiss) by
the in vitro detection of a respiratory burst enhancing cytokine-like factor. Abstract,
International Symposium on the Diseases of Aquatic Organisms, Seattle, Washington.
September 4-8, 1994.
Bootland, L. M., H. V. Lorz, B. S. Drolet, S-J. Chen, J. L. Fryer, J. S. Rohovec, and J. C.
Leong. 1994. Current status of studies on the life cycle of Infectious Hematopoietic Necrosis
Virus (IHNV). Abstract, International Symposium on the Diseases of Aquatic Organisms,
Seattle, Washington. September 4-8, 1994.
Leong et al. 1994. Molecular approaches to the control of diseases in fish. International
Symposium on Biotechnology Applications in Aquaculture. Taipei, Taiwan, ROC. December
5-10, 1994.
Leong Pubs, p. 17
Leong, J. 1995. Molecular biological tools to control diseases in fish. International
Symposium on Molecular Biology in Fish, Fisheries, and Aquaculture. Plymouth, U.K. July
10-13.
Leong et al. 1996. Fish Vaccine Antigen Produced or Delivered by Recombinant DNA
Technology. Symposium on Fish Vaccinology. Oslo, Norway. June 5-7.
Leong, J. 1997. Viral Vaccines for Fish. SeaGrant Marine Biotechnology Briefing.
Washington, D.C. March 1.-3, 1997.
Leong, J. et al. 1997. Fish Viral Vaccines and Adjuvants for Salmonid Aquaculture. World
Aquaculture 97. Seattle, Washington. February 19-23, 1997.
Leong, J. 1997. Viruses in Aquaculture: Biotechnological Control Strategies. Biotech ’97.
Networking for Success. Toronto, Canada. March 11-13, 1997.
Leong, J. 1997. Fish Vaccines and Immunoadjuvants. 7th Society of Chinese Bioscientists
of American International Symposium. Organizer: Choy Hew. Toronto, Canada. July 6-13,
1997.
Leong et al. 1997. Immune Response to Viral Diseases in Fish. 7th Congress of the
International Society of Developmental and Comparative Immunologists. Organizers: R.
Cipriano, M. Faisal, S. Kaattari, S. Ristow, and G. Vasta. College of William and Mary,
Williamsburg, Virginia. July 21-25, 1997.
Leong et al. 1997. Fish Viral Vaccines and Adjuvants for Salmonid Aquaculture. First
International Veterinary Vaccines and Diagnostics Conference. Organizer: R. Schultz.
Madison, Wisconsin. July 27-31, 1997.
Leong, J. C., and Scott E. LaPatra. 1998. Fish Disease Review, United States: Pacific
Region. All-Russia/United States Bilateral Symposium: Aquaculture and Fish Health 1998.
Rybnoe, Dmitrov Region, Russia. July 11-20, 1998
Leong et al. 1998. Development of sub-unit vaccines against fish viral pathogens.
Russia/United States Bilateral Symposium. Aquaculture and Fish Health. 1998. Rybnoe,
Dmitrov Region, Russia. July 11-20, 1998.
Leong et al., 1998. Rational Immunotherapy for fish vaccines. Marine Aquaculture
Workshop. Stamford, Connecticut. June 26-27, 1998.
Leong et al., 1998. Molecular tools for the diagnosis of fish diseases. Northeastern Regional
Aquaculture Workshop. Rockport, Maine. November 18-21, 1998.
Leong et al. 1999. Genetic Vaccination of Rainbow Trout. Proceedings of the 8 th
International Sympoisum of the Society of Chinese Bioscientists of America. Hong Kong,
August 14-19, 1999.
Leong et al., 1999. New Technology in Prevent of Viral Diseases. Proceedings of
Aquaculture Europe ’99. Trondheim, Norway, August 7-10, 1999.
M. Yoshimizu, Y. Hori, T. Yoshinaka, T. Kimura, and J. Leong. 2000. Evaluation of methods
used for the surveillance and monitoring of fish health for risk assessment. Abstract.
Proceedings of the meeting, “International Conference on Risk Analysis in Aquatic Animal
Health” sponsored by OIE. Feb. 2000, Paris.
Leong Pubs, p. 18
J. Leong et al., 2000. Development of DNA Vaccines for Fish. Third International
Symposium of the Japan Aquaculture Society. The New Paradigm for Aquaculture. Feb. 811, 2000 Mihama-ku, Chiba City.
B. Simon and J. Leong. 2000. Attenuated Bacterial Delivery system for DNA Vaccines. 8 th
International Symposium on Developmental and Comparative Immunology. Cairns,
Australia. July 2-7, 2000.
M. Alonso and J. Leong. 2000. Development of fish promoters for DNA vaccination. 8 th
International Symposium on Developmental and Comparative Immunology. Cairns,
Australia. July 2-7, 2000.
E. Thomann and J. Leong. 2000. Mx expression as a biomarker for immune competence in
fish. 8th International Symposium on Developmental and Comparative Immunology. Cairns,
Australia. July 2-7, 2000.
W. M. Contreras-Sanchez, M. S. Fitzpatrick, M. Alonso, C.B. Schreck, and J. C. Leong. 2000.
Identification of unique genes expressed during sex inversion of Nile tilapia (Oreochromis
niloticus) induced by short immersions in the synthetic steroid Trenbolone Acetate.
International Symposium on Fish Endocrinology. Sept. 2000 Aberdeen, Scotland.
R. M. Milston, A. T. Vella, T. Crippen, C. B. Schreck,, J. C. Leong, and M. S. Fitzpatrick.
2000. In vitro detection of humoral immunocompetence of juvenile salmon by flow cytometry.
International Symposium on Fish Endocrinology. Sept. 2000, Aberdeen, Scotland.
J. C. Leong, J. Drennan, M. Johnson, D. Jordan, C. Kim, B. Simon, and E. Thomann. 2000.
DNA Vaccination and Disease Control in Aquaculture. International Marine Biotechnology
Conference. Townsville, Australia. Sept. 29-Oct. 4, 2000.
J. C. Leong, M. Alonso, L. Bootland, T. Crippen, M. Fitzpatrick, D. Jordan, D. Leisy, R.
Milston, Carl Schreck, B. Simon, E. Thomann, and Anthony Vella. 2000. Biomarkers of
Immune Induction in Fish. International Congress of Veterinary Medicine. October 2729,2000. Puerto Varas, Chile.
J. C. Leong, M. Alonso, L. Bootland, D. Jordan, D. Leisy, B. Simon, and E. Thomann. 2000.
DNA vaccines for IHNV and IPNV. International Congress of Veterinary Medicine. October
27-29, 2000. Puerto Varas, Chile.
J. C. Leong, M. Alonso, D. Leisy, B. Simon, and E. Thomann. Viral Diseases in Aquaculture:
Impact and Control Measures. The Asia Pacific Conference on Plant Tissue Culture &
Agriciotechnology, November 19-23, 2000. Singapore
Leong, J. C. et al., 2001. Genetic Vaccines for Aquaculture. Aquaculture Biotechnology:
Interface for Maximum Impact. March 5-7, 2001. National Conservation Training Center.
Shepherdstown, West Virginia.
Schwindt, A.R., Leong, J. C., and Schreck, C.B. 2002. Stress induced differential gene
expression in the brains of juvenile steelhead trout (Oncorhynchus mykiss). American
Fisheries Society, New Orleans, LA.
Schwindt, A.R., Leong, J. C., and Schreck, C.B. 2002. Stress induced differential gene
expression in the brains of juvenile streelhead trout (Oncorhynchus mykiss). International
Congress on the Biology of fish. July 21-22, 2002. University of British Columbia,
Vancouver, Canada.
Leong Pubs, p. 19
Misumi, I., T. D. Lewis, J. C. Leong. 2006. Immmune Response of Fish Against Cryptocaryon
irritans and Identification of An Antigenic Parasite Protein. Joint Seminar 2006. National
University Cooperation. Tokyo University of Marine Science and Technology. October 13, 2006.