CURRICULUM VITAE
Name: Johanna Jeanine Sloss (Fryman)
Address: 1709 Elk Street
Stevens Point, WI 54481
Telephone: 715-498-2873
E-mail: slossconsulting@att.net
Education:
Devry University, Keller Graduate School of Management, MBA, August 2009- present
Southern Illinois University, Carbondale, IL, B. A. Microbiology, May 2000
Olney Central College, Olney, IL, Assoc. of Arts, Assoc.of Science, May 1995
Certifications:
Molecular Biology Specialist, MB (ASCP)CM, 8/2009
Professional Experience:
Research and Development, Molecular Pathology Department, Marshfield Clinic,
Marshfield, WI, 11/05-present
Clinical Laboratory Technician, Molecular Biology Department, Marshfield Clinic,
Marshfield, WI, 04/02-11/05
Research Technician I, Howard Hughes Medical Institute, University of Cincinnati,
Department of Molecular Genetics, Cincinnati, OH, 1/02-4/02
Research Assistant, University of Cincinnati, Department of Molecular Genetics,
Cincinnati, OH, 2/01-1/02
Research Technician II, Washington University School of Medicine, Genome
Sequencing Center, St. Louis, MO, 5/00 – 12/00
Student Researcher, Dr. Laurie Achenbach/Dr. John Coates, Microbiology Department,
Southern Illinois University, Carbondale, IL, 8/98 – 5/00
Field Researcher, Monsanto, Grand Island, NE, 5/98 – 8/98
Field Researcher, Alvey Labs, Mt. Carmel, IL, 5/97 – 11/97
Other:
SIUC Microbiology Student Organization, President, 5/99 – 5/00
Undergraduate Student Advisory Committee – Microbiology Representative
Molecular Pathology Contract Speaker, Wisconsin
College of American Pathologists Molecular Inspector, 2010
Independent Consultant, Molecular, 2010
ASCP Local Representative for the Midwest Region, 9/2010
Laboratory duties:
Supervised and trained new employees
Supervised and trained individuals in clinical applications
Cost accounting
Quality Control parameters
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Advise on marketing molecular assays
Molecular biology CAP preparation and inspection liaison
Supervised Medical Technology student rotation in department
Evaluated Medical Technology student progress
Collaborate with other departments in molecular development
Prepared work schedules for department
Prepared and updated laboratory protocols
Experience with laboratory information systems
Development of new molecular based clinical tests
Provide input to identify opportunities for process improvement
Troubleshooting and problem resolution of clinical tests
Test packet preparation of clinical tests
Maintained inventory and ordering
Communicate with Clinical Sales Representatives
Negotiate with Clinical Sales Representatives
Supervised and trained individuals in manual DNA sequencing, PCR and mouse
husbandry skills
Lead Technician- Cobas Amplicor System
Laboratory Skills:
Polymerase Chain Reaction
Prokaryotic cloning
Mouse Husbandry Skills
Manual DNA sequencing
Automated DNA sequencing protocols
Primer design
Media and reagent preparation
Computer-aided sequence alignment
RNA isolation
Tissue culture
Southern Blot
Western Blot
Northern Blot
cDNA
Light Cycler applications
Cobas Amplicor Instrument System
Real-Time Instrument Systems
Automated DNA and RNA extraction systems
Clinical tests:
Human papillomavirus identification
Hepatitis C quantitative PCR
Hepatitis C qualitative PCR
HIV quantitative PCR
Chlamydia trachnomatis PCR
Neisseria gonorrhea PCR
Factor V Lieden gene mutation
Prothrombin gene mutation
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Hepatitis B quantitative PCR
Cystic Fibrosis
Herpes simplex I & II PCR
Bordetella pertussis and B. parapertussis PCR
Borrelia burgdorferi PCR
Varicella zoster PCR
Hepatitis C genotyping
Alpha-1-Anti-trypsin PCR
Clostridium difficile PCR
Methicillin-resistant Staphylococcus aureus PCR
Test Validation:
Hepatitis B quantitative PCR
Hepatitis C quantitative PCR
HIV quantitative PCR
Cystic Fibrosis
Bordetella pertussis and B. parapertussis PCR
Alpha-1-Anti-trypsin PCR
Extraction automation for various sample types
Various DNA extraction changes for current testing platforms
Lyme Disease Western blot
Herpes simplex 1& 2 PCR
Clostridium difficile PCR
Methicillin-resistant Staphylococcus aureus PCR
KRAS mutation analysis (Codons 12, 13, 61)
Memberships in Professional Organizations:
American Society of Microbiology
American Society for Clinical Pathology
Association for Molecular Pathology
Clinical and Laboratory Standards Institute
American Society for Clinical Laboratory Science
Publications:
Uphoff, T. and J. Sloss. 2010. Molecular Diagnostic Testing for Varicella-Zoster Virus
Infections Expands to Swab Samples from Skin and Mucosal Lesions. Marshfield
Clinic Laboratory News 33:7.
Uphoff, T. and J. Sloss. 2010. Molecular Diagnostic Testing for Herpes Simplex Virus
Infections Expands to Swab Samples from Skin and Mucosal Lesions. Marshfield
Clinic Laboratory News 33:4.
Uphoff, T. and J. Sloss. 2008. New Testing Methods Expand the Reportable Range for
Hepatitis B and Hepatitis C Virus Quantitative Assays. Marshfield Clinic
Laboratory News 31:7.
Uphoff, T. and J. Sloss. 2007. Laboratory Testing for Alpha-1-Antitrypsin Deficiency
[AATGENO]. Marshfield Clinic Laboratory News 30:6.
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Dokken, C., J. Fryman, C. Hipke, B. Voelker, K. Reed, B. Krawisz. October 27, 2002.
Detection of Human Papillomaviruses and Its Usefulness in Interpreting Results
of Cervical Smears. Marshfield Clinic Laboratory News, 25: 5
Achenbach, L. A., U. Michaelidou, R. A. Bruce, J. Fryman, and J. D. Coates. 2001.
Dechloromonagitata gen. nov., sp. nov. and Dechlorosomaillum gen. nov.,
sp. nov., two novel environmentally dominant (per)chlorate-reducing bacteria and
their phylogenetic position. International Journal of Systematic and Evolutionary
Microbiology 51: 527-533.
Kazusa DNA Research Institute, The Cold Spring Harbor and Washington University
Sequencing Consortium, The European Union Arabidopsis Genome Sequencing
Consortium & Institute of Plant Genetics and Crop Plant Research (J. Fryman
among 128 authors). 2000. Sequence and analysis of chromosome 5 of the plant
Arabidopsis thaliana. Nature 408: 823-826.
GeneBank Human Genome Project, Washington University Genome
Sequencing Center: AC019226.5, AC068919.4, AC019171.4, AC073962.5,
AC023344.4, AC021012.5, AC012062.4, AC010139.4, AC017093.3
Presentations:
Sloss, J.J., M. F. Vandermause, J.K. Meece, and T.S Uphoff. June 2010. Evaluation of
Three Multiplex RT-PCR Assays for Influenza A, Influenza B, and Respiratory
Syncytial Virus Detection. Marshfield Clinic 2010 Medical Education Day,
Marshfield, WI.
Sloss, J.J., M. F. Vandermause, J.K. Meece, and T.S Uphoff. May 2010. Evaluation of
Three Multiplex RT-PCR Assays for Influenza A, Influenza B, and Respiratory
Syncytial Virus Detection. 2010 American Society for Microbiology General
Meeting, San Diego, CA.
Uphoff, T., J. Sloss, M. E. Stemper, T. Novicki, and T. Fritsche. April 2010. Comparison
of three real-time PCR assays for toxigenic Clostridium difficile with a two-tiered
rapid immunochromatography algorithm. 26th Annual Clinical Virology
Symposium, Daytona, FL.
Sloss, J.J. September 2007. DNA Technology in the Clinical Laboratory. 16th Annual
Symposium Review. Molecular Pathology Department, Marshfield Labs.
Marshfield, WI
Fryman, J., L. A. Achenbach, U. Michaelidou, R. A. Bruce, and J. D. Coates. March,
2000. Phylogenetic analysis of dissimilatory (per)chlorate-reducing bacteria and
development of molecular probes. 2000 Cellular and Molecular Biology
Symposium, Southern Illinois University, Carbondale, IL.
Michaelidou, U., L. A. Achenbach, S. O’Connor, J. Fryman, and J. D. Coates. 2000.
The diversity and ubiquitous microbiology of perchlorate reduction. American
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Water Works Association meeting, Albuquerque, NM.
Relevant Science Course Work:
Elementary Microbiology
Basic Genetics
Principles of Microbiology
Medical Microbiology
Undergraduate Independent Research
Prokaryotic Diversity
Diagnostics and Applied Microbiology
Human Gross Anatomy
Organic Chemistry
Inorganic Chemistry
Molecular Biology
Biotechnology
Genetics of Bacteria and Viruses
Molecular Biology of Microorganisms Lab
Anatomy and Physiology
Advanced Anatomy and Physiology
Relevant Business Course Work:
Managerial Application of Information Technology
Legal, Political and Ethical Dimensions
Leadership and Organizational Behavior
Business Economics
Marketing
Business Statistics
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References:
Available upon request.