Molecular Biology: PCR techniques
Molecular Biology:
PCR Techniques
Author: Prof Estelle Venter
Licensed under a Creative Commons Attribution license.
REFERENCES
Multimedia
A multimedia programme on CD ROM demonstrating practical skills is available: ‘Molecular Biology
and Recombinant DNA-technology’. Developed by the Department of Veterinary Tropical Diseases.
Websites
1.
http://www.rothamsted.bbsrc.ac.uk/notebook/courses/guide/
2.
http://highveld.com/f/fpcr.html
3.
http://www.protocol-online.org/
4.
http://www.dnaftb.org/dnaftb/
5.
http://irc.igd.cornell.edu/Protocols/PCR_principles.htm
6.
http://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechPCR.shtml
7.
http://www.maxanim.com/genetics/PCR/PCR.htm
8.
http://www.sumanasinc.com/webcontent/animations/content/pcr.html
9.
http://molecular.roche.com/About/pcr/Pages/ApplicationsofPCR.aspx
10.
http://www.promega.com/country.aspx?returnurl=http%3A%2F%2Fwww.promega.com%2Fres
ources%2Fproduct-guides-and-selectors%2Fprotocols-and-applications-guide%2Fpcramplification%2F
Laboratory Manuals
1.
Molecular Cloning, A Laboratory Manual, 2 nd edition. J.Sambrook, E.F. Fritsch and T. Maniatis.
Cold Spring Harbor Laboratory Press. 1989.
2.
PCR Applications Manual, Boehringer Mannheim. ©1995 by Boehringer Mannheim GmbH.
Biochemica
3.
Roche Molecular Biochemical’s. PCR Applications Manual, 2 nd edition. © Roche diagnostics,
1999 GmbH, Mannheim.
4.
PROMEGA: Protocols and applications guide. 1996
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Molecular Biology: PCR techniques
Books/Articles
1.
BROWN, T A 1986. Gene cloning. Van Nostrand Reinhold (UK) Co. Ltd.
2.
BROWN, T A 1995 3rd Edition. Gene cloning – An introduction. Chapman & Hall, London.
3.
D’AQUILA, RT, BECHTEL, L.J.,VITELER, J. A., ERON, J.J., GORCZYCZ, P. and KAPLIN,
J.C., 1991. Maximizing sensitivity and specificity of PCR by preamplification heating. Nucleic
Acids Res. 19: 3749.
4.
DIEFFENBACH, C. W. and DVEKSLER, G. S. 1995. PCR primer. A laboratory manual. CSHL
Press.
5.
EHRLICH, H.A, GELFAND, D. and SNINSKY, J.J., 1991. Recent advances in the polymerase
chain reaction. Science 252: 1643 – 1651.
6.
HYDE, J 1990. Molecular parasitology. Van Nostrand Reinhold. New York
7.
KWOK, S. and HIGUCHI, R 1989. Avoiding false positives with PCR. Nature 339: 237 – 238.
8.
LONGO, MC., BERNINGER, MS. and HARTLEY JL. 1990. Use of uracil DNA glycosylase to
control carryover contamination in polymerase chain reactions. Gene 93: 125 – 128.
9.
MAXAM, AM and GILBERT, W 1980.
Sequencing end-labelled DNA with base-specific
chemical cleavage. Methods in Enzymology, 65: 499 –552.
10.
MULLIS, K B, 1991. The polymerase chain reaction in an anemic mode: How to avoid cold
oligodeoxy-ribonuclear fusion. PCR Methods Appl. 1: 1-4.
11.
SANGER, F. NICKLEN, S and COULSON, AR 1979. DNA sequencing with chain-terminating
inhibitors. Proc. Natl Acad. Sci. USA 74, 5463 – 5467.
12.
SMITH CA and WOOD EJ 1991. Molecular biology and biotechnology. Chapman & Hall,
London.
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