Austin Community College
MLAB 2401
Protein Electrophoresis
Clinical Chemistry
Name_____________________________
Date_______________
INSTRUCTIONS: Unless otherwise noted, each question is worth one point. Answer the questions in
an appropriate manner in the space provided.
1.
When proteins are placed in an appropriate buffer, what property allows them to be
electrophoresed?
2.
What buffer is routinely used to electrophorese proteins?
3.
AMoving boundary@ electrophoresis was introduced in 1937 by
A
Tiselius
B
Koning
C
Kjeldahl
D
Van den Bergh and Muller
4.
Identify the five (5) major protein classes, listing them in order of migration from the anode to
the cathode.
5.
The principle of electrophoresis is based on the fact that a charged particle in an electrical field
will migrate toward one of the electrodes, depending on a number of variables. List at least two
(2) of the variables stated in the lab.
6.
What purpose is served by the Ponceau S in this procedure?
7.
Which protein fraction is most affected by a phenomenon known as electroendosmosis?
8.
Calculate the concentration of each protein fraction for the following electrophoresis specimen.
Use correct units.
Total Protein
100%
Albumin
45%
alpha 1
8%
alpha 2
10%
beta
21%
GAMMA
16%
8.9 gm/dL
9.
What is the main physiologic function provided by albumin?
10.
What is the fastest moving protein fraction using this electrophoresis procedure?
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Austin Community College
MLAB 2401
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