STANDARD OPERATING PROCEDURE FOR THE USE OF
ELECTROPHORESIS EQUIPMENT
Electrophoresis involves the use of high voltages and carries the risk of
electric shock.
Many chemicals commonly used in electrophoresis are highly toxic.
1. Make sure you have been trained by an experienced worker in
the safe use of electrophoresis equipment.
2. Familiarise yourself with the COSHH forms for the chemicals you
intend to use.
3. Check equipment and wiring before use. Look for signs of
damage. Do not use worn or frayed leads.
4. Use only electrophoresis tanks which have a secure design
preventing contact with buffer when connected to a power
supply.
5. Always disconnect from the power supply before opening.
6. Switch off power before moving a tank.
7. Clean up spills of electrophoresis buffer or gel mixes immediately
– these may contain toxic chemicals e.g. ethidium bromide or
acrylamide.
8. Latex gloves often contain small holes – use nitrile (or other
suitable) gloves when immersing hands in electrophoresis
buffers or handling gels.
9. When using vertical electrophoresis equipment, take care that
leakage from the upper buffer chamber does not cause arcing.
Emergency action in case of electric shock
1. Switch off power at once. Do not attempt to touch the victim until
they have been isolated from the source of electricity. If you are
unable to get to the power supply you will have to insulate yourself
so that you do not become a casualty. A piece of DRY wood or rolled
up paper could normally be used.
2. Check the casualty’s airway and breathing if unconscious be ready
to resuscitate, call for help immediately
DL/JH 2001
(From a University phone dial 222 or from a brown phone dial R999)
3. Treat any burns when away from the electricity by flooding the
affected part with water for a minimum of 10 minutes. Remove and
constrictions if swelling is likely, keep injury elevated.
Do not burst blisters, apply any creams or sprays or apply any
plasters. Seek help from a first aider.
DL/JH 2001