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Advanced Biology
1. STAT family proteins, transcription factors, are activated in
(a) Nucleus, (b) Golgi apparatus,
(c) Cytoplasm, (d) Mitochondria.
[1]
2. Bcl2 family proteins are involved in
(a) Cell death,
(b) Cell survival,
(c) Maintenance of cell,
(d) Transformation of cell
[1]
3. Explain schematically how EGF-EGFR signaling pathway involves in proliferation and
survival of mammalian cells?
[3]
4. What are the differences between a normal cell and a transformed cell?
[3]
5. Explain with example the use of fusion proteins in protein-protein interaction
[3]
6. Define different types of stem cells? What are the advantages to culture stem cells? [3]
7. State True or False
(a)
(b)
(c)
(d)
(e)
[1x5]
T-cells produce antibodies
Dendritic cells are antigen presenting cells
Hapten alone is immunogenic
IgG is a pentamer
Edward Jenner developed vaccine against rabies
8. ADP-ribosylation of proteins is carried out with the help of the small covalent group: [1]
[a]
ATP
[b]
SAM
[c]
NAD
[d]
None of the above
9. NAD+-dependent deacetylases are involved in:
[a]
Chromosome stability
[b]
Chromosome instability
[c]
Gene duplication
[d]
Genetic recombination
10. Phosphorylation of a polypeptide usually occurs at:
[a]
Glycine
[b]
Serine
[c]
Lysine
[d]
Leucine
11.
[1]
[1]
Which type of post-translational modification regulates acetyl-CoA synthetase of
Slamonella entarica?
[1]
a) Phosphorylation ; b) Methylation
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(c) Acetylation;
d) None of the above
12.
Which organism uses Restriction modification system to protect themselves from
pathogens
[1]
(a) bacteria ;
(b) fungi
(c) enveloped viruses ; (d) All of them (a,b,c)
13.
Host defense molecules in plants are
[1]
(a) Hypersensitive response (b) Resistance" (R) proteins(c) Systemic acquired resistance
(d) All of them (a,b,c)
14.
Antimicrobial peptides are present in
Invertebrates(b) Vertebrates(c) Both of them (a,b) (d) None of them (a,b)
15.
Myeloid Antimicrobial Peptides are
Cathelicidins
(c) Indolicidins (d) All of three (a, b, c)
16.
[1] (a)
[1] (a) Defensins (b)
Antimicrobial Peptide is a
[1](a) Short, generally
anionic, broad-spectrum antimicrobial proteins(b) Long, generally anionic, broadspectrum antimicrobial proteins
(c) Short, generally cationic, broad-spectrum antimicrobial proteins
(d) Long, generally cationic, broad-spectrum antimicrobial proteins
17.
Defensins act on :
(a) bacteria ; (b) fungi
(b) (c) enveloped viruses ; (d) All of them (a,b,c)
[1]
18. Name four important properties of Stem cells
[3]
19. What is a hematopoietic stem cell?
[3]
20. The first differentiation marker of primitive human haematopoietic stem cell (HSC) is [1]
a) CD33 ; b) CD38 ; c) CD34.
21. What do you mean by cell plasticity?
[2]
22. Mentions a few steps for the Isolation of normal haematopoietic stem cell (HSC) from bone
marrow
[3]
23. How do you enriched stem cell population from mononuclear cells (MNCs) by negative
selection methods?
[2]
24.
DNA-protein interaction is studied by:
(a) Northern hybridization, (b) Real time PCR, (c) EMSA, (d) DD-PCR.
[1]
25.
If you want to identify the target genes of a transcription factor in a particular tissue,
which technique you would use?
[1]
(a) Subtractive hybridization, (b) Chromatin Immunoprecipitation (ChIP), (c) Western
Blotting, (d) Co-immunoprecipitation.
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26.
General Transcription Factors bind to which part of a gene to facilitate binding of RNA
Polymerase II?
(a) Promoter, (b) Intron, (c) Exon, (d) None of the above[1]
27.
Gene activity is repressed by
[1]
(a) Histone deacetylation, (b) Histone acetylation, (c) Both, (d) Neither of the
two.
28.
Differential expression of a gene (at different conditions) can be most
accurately and quantitatively studied by
[1]
(a) Northern hybridization, (b) Reverse transcription-PCR, (c) Real-Time RTPCR, (d) DD-PCR.
29.
Are these antioxidants? (Tick the correct one. )
[1]
i. Vitamin E ; ii. Glutathione
iii. Thioredoxin ; iv. Superoxide dismutase ; v. All of the above
30. Which caspase is specifically activated in the mitochondrial pathway of
apoptosis?
[1]
i. Caspase 3 ; ii. Caspase 8
iii. Caspase 9 ; iv. Caspase 12
31. State whether the following statements are true / false : [1x2]
a. The release of cytochrome c from mitochondria is a primary event in the death
receptor pathway of apoptosis.
b. AIF and EndoG can induce caspase independent cell death.
32. When a functional protein becomes inactive under normal physiological conditions, does it
unfold?
33. In the energy funnel concept of protein folding, what prevents a protein molecule from perfect
folding?
34. How does a beta-sheet structure of a protein confers 'global stability' of the molecule?
35. Draw an energy versus reaction coordinate sketch between the folded and unfolded states of
a protein.
36. For an enzyme, is it necessary that its pH-optima and pH-stabiilty patterns should be similar?
37. CD127 is the receptor for: a) IL-3 ; b) IL-4 ; c) IL-7
38. FoxP3-deficient animals: a) lack T reg cells ; b) have higher number of T reg cells
c) have unaffected T reg cells
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39. Intracellular staining is required for staining with Fox P3-specific antibody:
a) Yes b) No c) does not matter
40. Expression of CD127 on T reg cells is : a) low ; b) high
41. State True or False
a). Antibodies specific for CD11c stain macrophages.
b). Antibodies specific for CD11b stain for dendritic cells.
c). Antibodies specific for CD11c stain for dendritic cells.
42. State True or False
a). Macrophages in the lamina propria induce the differentiation of Foxp3+ regulatory
cells.
b). Lamina propria dendritic cells induce the differentiation of Foxp3+ regulatory cells.
c). Macrophages in the spleen induce the differentiation of Foxp3+ regulatory cells.
43. State True or False
a).Lamina propria macrophage derived IL-10 exerts immunosuppressive effects on T
cells.
b). Lamina propria dendrites promote T-cell IL-17 production.
c).Lamina propria macrophages limit the ability of intestinal dendritic cells to promote
IL-17 producing cells.
44. What is the longest phase during Cell cycle ?
(i)
G1
(ii)
G2
(iii)
M
(iv)
S
45. Cdk activity is suppressed by
(i)
(ii)
(iii)
(iv)
Cdc 25
Wee 1 kinase
P27 kinase
Both P27 kinase and wee1 kinase
46. The absence of p53 will result into
(i)
(ii)
(iii)
(iv)
Insensitive to UV radiation
Very sensitive to UV radiation
Metastatis
Apoptosis
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