1 Supplementary figure 1 The brain region used for the identification of a gene that controls photoperiodic time measurement by differential subtractive hybridization analysis (dashed line). Cb: cerebellum; IN: infundibular nucleus; ME: median eminence; NHPM: nucleus hypothalamicus posterior medialis; OC: optic chiasm; POA: preoptic area; SCN: suprachiasmatic nucleus; P: pineal gland. Supplementary figure 2 Detailed localization of D2 expression in the mediobasal hypothalamus (MBH). Acute induction of D2 was observed in the dorsal hypothalamus (DH) around the paraventricular organ (PVO) and lateral hypothalamus (LHy) in the rostral region (a) and the caudal region (b) of the MBH. The outline of LHy is shown by dotted circles12. Strong signal was observed in the infundibular nucleus (IN) and median eminence (ME) under LD (c). IN and ME are also called basal tuberal hypothalamus (BTH). CO: optic chiasma; VMN: nucleus ventromedialis hypothalami; DSD: decussatio supraoptica dorsalis; DMN: nucleus dorsomedialis hypothalami; IH nucleus inferioris hypothalami; Supplemental figure 3 Expression of transthyretin (TTR) in the Japanese quail brain. Strong signals of TTR were observed in the choroid plexus (CP) both under LD (a) and SD (b), while no signal was detected in the hypothalamus (arrowhead). No significant difference in the TTR expression between LD and SD was observed (c) (Mann-Whitney U-test, p>0.05).