Spring 2012 Agriscience Midterm Name (print large and clearly): Date: Hr: Directions: Do NOT open this packet until instructed to do so. There should be absolutely NO TALKING during the exam unless you have raised your hand and have been called on by the instructor. Failure to do so will result in one verbal warning, and then a failed test grade. Students should sit on opposite sides of the lab tables for this exam. DO NOT sit together on the same side. Select the BEST answer to each question from the options given (more than one may sound right, but there is only one entirely correct answer per question). Be sure to clearly mark your answers on the Scantron sheet. You may write on your test packet, but only answers on the Scantron will be considered. You may use one and only one 3x5” note card with your own handwritten note. This notecard must stay on the desk in front of you at all times. Exam test taking tips: Treat each question like a short answer question if you can. Cover the answers, create an answer in your head, and then match that answer to those given to find the best answer. If you do not know an answer, skip it and come back later. Use information from other questions to help you. One question may help you answer another question. Cross off answers to eliminate and lower possibilities. If you cannot narrow the options to just one, select the answer that sounds the best to you and makes the most sense. Read each question AND each option carefully. Do not select an answer until you have read them all! Good Luck! Copyright 2012 by Craig Kohn, Agricultural Sciences, Waterford WI. This source may be freely used and distributed provided the author is cited. 1. An animal that has both recessive alleles would be described as… a. Heterozygous b. Homozygous Dominant c. Homozygous Recessive d. Co-dominant e. Incompletely Dominant 2. An animal that has both dominant alleles would be described as… a. Heterozygous b. Homozygous Dominant c. Homozygous Recessive d. Co-dominant e. Incompletely Dominant 3. An animal that has a recessive allele and a dominant allele would be described as… a. Heterozygous b. Homozygous Dominant c. Homozygous Recessive d. Co-dominant e. Incompletely Dominant 4. If two different traits are both equally expressed in an individual’s phenotype, those traits would be considered… a. Heterozygous b. Homozygous Dominant c. Homozygous Recessive d. Co-dominant e. Incompletely Dominant 5. If two different traits sort of blend, so that neither is fully present in the phenotype, those traits are… a. Heterozygous b. Homozygous Dominant c. Homozygous Recessive d. Co-dominant e. Incompletely Dominant 6. An organism that expresses a recessive phenotype must be… a. Heterozygous b. Homozygous Dominant c. Homozygous Recessive d. Co-dominant e. Incompletely Dominant 7. Epistasis is... a. The physical appearance of an organism that is the result of its genes. b. The combination of genes in an organism. c. When one gene affects the expression of another gene. Copyright 2012 by Craig Kohn, Agricultural Sciences, Waterford WI. This source may be freely used and distributed provided the author is cited. d. The term for a version of a gene 8. A genotype is… a. The physical appearance of an organism that is the result of its genes. b. The combination of genes in an organism. c. When one gene affects the expression of another gene. d. The term for a version of a gene 9. An allele is… a. The physical appearance of an organism that is the result of its genes. b. The combination of genes in an organism. c. When one gene affects the expression of another gene. d. The term for a version of a gene 10. A phenotype is… a. The physical appearance of an organism that is the result of its genes. b. The combination of genes in an organism. c. When one gene affects the expression of another gene. d. The term for a version of a gene 11. If all of the offspring of a couple are the recessive phenotype, the parents must have which genotype combination? a. AA x AA b. Aa x Aa c. Aa x aa d. aa x aa 12. If 3/4s of the offspring have the dominant phenotype and ¼ have the recessive phenotype, the parent combination is most likely… a. AA x AA b. Aa x Aa c. Aa x aa d. aa x aa 13. If the offspring are half dominant, half recessive phenotypes, the parent combination is most likely… a. AA x AA b. Aa x Aa c. Aa x aa d. aa x aa 14. If all of the offspring are homozygous dominant, the parent combination must be… a. AA x AA b. Aa x Aa c. Aa x aa d. aa x aa 15. In Holstein cows, black is dominant and red is recessive. A bull and a cow (both heterozygous for color) have 3 calves. All of them are black. What are the odds that their fourth calf will be red? a. 0% b. 25% c. 50% d. 100% Copyright 2012 by Craig Kohn, Agricultural Sciences, Waterford WI. This source may be freely used and distributed provided the author is cited. A pair of frogs and their offspring are show to the side. Couples are denoted with the dotted boxes around them. Offspring (Couples 1-4) are below the couples (the offspring of the top pair are connected to them with a line; their mates are unrelated to the top pair of frogs). Top Pair Above each frog, write their genotype. Then answer the questions below. You will be able to determine the genotype for each frog based on the parents and the offspring. Couple 1 Couple 2 16. How many frogs on this page are homozygous recessive? a. 1 b. 2 c. 11 d. 13 e. 15 17. How many frogs were homozygous dominant? a. 1 b. 2 c. 11 d. 13 e. 15 18. How many frogs were heterozygous? a. 1 b. 2 c. 11 d. 13 e. 15 19. What are the genotypes of Couple 1? a. Gg x Gg b. GG x gg c. Gg x gg d. gg x gg e. GG x GG 20. What are the genotypes of Couple 2? a. Gg x Gg b. GG x gg c. Gg x gg d. gg x gg e. GG x GG 21. What are the genotypes of Couple 3? a. Gg x Gg b. GG x gg c. Gg x gg d. gg x gg e. GG x GG 22. What are the genotypes of Couple 4? a. Gg x Gg b. GG x gg c. Gg x gg d. gg x gg e. GG x GG Copyright 2012 by Craig Kohn, Agricultural Sciences, Waterford WI. This source may be freely used and distributed provided the author is cited. Couple 3 Couple 4 A farmer selects two goats to mate. The farmer wants to raise curly-horned goats. The first goat is polled (no horns). The other goat has curly horns. Polled is dominant to horns and straight horns are dominant to recessive. They have the following genotypes: Goat 1: PpHh Goat 2: pphh PP, Pp = polled; pp = horned HH, Hh = straight horns; hh= curly horns Polled = P _ _ _ Horned = pp _ _ Straight horns = ppHh or ppHH Curled horns = pphh Complete the dihybrid Punnett Square before continuing 23. What are the odds that any given goat baby will be polled? a. 16/16 b. 8/16 c. 6/16 d. 4/16 e. 2/16 24. What are the odds that any given baby will be straight horned? a. 16/16 b. 8/16 c. 6/16 d. 4/16 e. 2/16 25. What are the odds that any given baby will be curly horned? a. 16/16 b. 8/16 c. 6/16 d. 4/16 e. 2/16 26. Could two polled goats have a horned baby? a. Yes b. No 27. Could two horned goats have a polled baby? a. Yes b. No Copyright 2012 by Craig Kohn, Agricultural Sciences, Waterford WI. This source may be freely used and distributed provided the author is cited. 28. A gene is… a. A tightly-wound package of DNA b. A section of DNA that codes for a specific protein c. A visible trait d. All of the above 29. In the picture at the right, this is A a. Phosphate b. Nitrogenous Base c. Deoxyribose sugar d. Nucleotide C A B 30. In the picture at the right, this is B a. Phosphate b. Nitrogenous Base c. Deoxyribose sugar d. Nucleotide 31. In the picture at the right, this is C a. Phosphate b. Nitrogenous Base c. Deoxyribose sugar d. Nucleotide 32. In the picture at the right, this is D a. Phosphate b. Nitrogenous Base c. Deoxyribose sugar D d. Nucleotide 33. This item in the picture could represent Adenine, Cytosine, Thymine, or Guanine A. B. C. D. 34. This structure contains the 5’ and 3’ carbons that give DNA its sense of direction A. B. C. D. 35. Which of the following accurately indicates the correct pairing of bases? a. A – C; G – T b. A – G; C – T c. G – C; T – A d. G – G; C – C; A - T 36. Specific combinations of bases exist because… a. They can’t fit in any other combination b. They can’t bond in any other combination c. Both A and B d. None of the above are correct 37. Which has 3 bonding sites? a. Adenine & Thymine b. Cytosine & Guanine c. Adenine & Guanine d. Cytosine & Thymine 38. Which of the following is shown here? a. A purine b. A pyrimidine c. A leucine d. A genie Copyright 2012 by Craig Kohn, Agricultural Sciences, Waterford WI. This source may be freely used and distributed provided the author is cited. Use the picture at the right for the following questions. 39. This structure is DNA A. B. C. D. 40. This structure is mRNA A. B. C. A B D. 41. This structure is the ribosome A. B. C. D. 42. This structure is tRNA A. B. C. D. 43. This structure is created by polymerase A. B. C. D. 44. This structure is opened by helicase A. B. C. D. 45. This structure delivers amino acids A. B. C. D. C 46. This structure reads copies of DNA and creates proteins A. B. C. D. 47. How does RNA differ from DNA? a. The G’s in DNA become U’s in RNA b. DNA is double stranded; RNA can be single stranded c. RNA has an extra –OH molecule d. All of the above are correct D B 48. A codon is a…. a. String of amino acids b. A kind of amino acid c. The structure that reads mRNA and makes a protein d. A group of three nucleotide bases 49. Which would be the correct mRNA version of this strand of DNA? 3' CCC-GTA-ATG-GCA-TAA-ATC 5' a. b. c. d. 3’ CCC – GTA – ATG – GCA - TAA - ATC 5’ 5’ CCC – GTA – ATG – GCA – TAA - ATC 3’ 3’ GGG – CAU – UAC – CGU – AUU - UAG 5’ 5’ GGG – CAU – UAC – CGU – AUU - UAG 3’ 50. What is the correct amino acid sequence for the mRNA above? a. Gly – His – Tyr – Arg – Ile – Term b. Term – Ile – Arg – Tyr – His – Gly c. Ile – Leu – Cys – His – Tyr – Gly Copyright 2012 by Craig Kohn, Agricultural Sciences, Waterford WI. This source may be freely used and distributed provided the author is cited. d. Ala – Asp – Glu – Gly – Phe – Leu 51. Which of the following amino acids forms a special bond called a disulfide bond, a bond which causes it to bind to other amino acids that are the same kind as itself? a. Glutamine b. Tyrosine c. Serine d. Cysteine 52. The shape of a protein determines its… a. Amino acid sequence b. Function c. Lifespan d. Location 53. Hydrophilic amino acids will always move to the a. Inside b. Outside c. Top d. Bottom of a protein 54. Hydrophobic amino acids will always move to the a. Inside b. Outside c. Top d. Bottom of a protein 55. Oppositely charged amino acids will always… a. Bond with each other b. Repel each other c. Move to the inside d. Move to the bottom 56. Similarly charged amino acids will always… a. Bond with each other b. Repel each other c. Move to the inside d. Move to the bottom 57. The primary structure of an amino acid refers to… a. The overall structure of the protein b. The combination of alpha helixes and beta sheets c. The order of amino acids d. The formation of an alpha helix or a beta sheet 58. The tertiary structure of an amino acid refers to… a. The overall structure of the protein b. The combination of alpha helixes and beta sheets c. The order of amino acids d. The formation of an alpha helix or a beta sheet 59. A frameshift mutation is one that… a. Causes a deletion b. Causes an insertion c. Causes all of the bases downstream to shift d. Causes no change to the protein structure 60. A frameshift mutation will change the of a protein a. Shape b. Function c. Secondary and Tertiary structure d. All of the above Copyright 2012 by Craig Kohn, Agricultural Sciences, Waterford WI. This source may be freely used and distributed provided the author is cited. 61. Which of the following is shown in X to the right? a. Alpha Helix b. Beta Sheet c. Amino Acid d. Polypeptide 62. Which of the following is shown in Y to the right? a. Alpha Helix b. Beta Sheet c. Amino Acid d. Polypeptide 63. X and Y together would make which of the following? a. Alpha Helix b. Beta Sheet c. Amino Acid d. Polypeptide 64. Which of the following would be the correct transcribed mRNA molecule for the DNA sequence below? . 3’ a. b. c. d. TAC-TTA-CGA-TGG-TAC-ACG-TGT-ACC-TTG-AAC-CTG-ACT 5’ 5’ – ATG-AAT-GCT-ACC-ATG-TGC-ACA-TGG-AAC-TTG-GAC-TGA- 3’ 5’ – AUG-AAU-GCU-ACC-AUG-UGC-ACA-UGG-AAC-UUG-GAC-UGA- 3’ 3’ – ATG-AAT-GCT-ACC-ATG-TGC-ACA-TGG-AAC-TTG-GAC-TGA- 5’ 3’ – AUG-AAU-GCU-ACC-AUG-UGC-ACA-UGG-AAC-UUG-GAC-UGA- 5’ 65. Which of the following would be the correct order of translated amino acids from the mRNA strand above? a. Met – Asn – Ala – Thr – Met – Cys – Thr – Trp – Asn – Leu – Asp b. Ser – Gln – Val – Gln – Gly – Thr – Arg – Val – Pro – Ser- Term c. Asn – Ala – Thr – Met – Trp – Pro – Arg – Val – Met – Asp – Trp d. Ile – Phe – Ser – Cys – His – Arg – Val – Ala – Asp – Glu – Leu – Term Copyright 2012 by Craig Kohn, Agricultural Sciences, Waterford WI. This source may be freely used and distributed provided the author is cited. 66. How many chromosomes do humans have in their cells? a. 46 – 23 from their father and 23 from their mother b. 23 – all from their mother c. 23 – all from their father d. 23 – either from their mother or from their father (it’s up to chance) 67. Chromosomes are… a. Bundles of DNA b. Proteins c. The nucleus of the cell d. All of the above 68. What was the contribution of Darwin to modern genetics? a. He showed how patterns of inheritance work (e.g. dominance vs. recessiveness) b. He demonstrated that species can change through natural or artificial selection c. He showed that DNA is the source of all genetic material d. He invented the butterfat test that enabled dairy farmers to create a genetic basis for improving their herds 69. What was the contribution of Watson and Crick to modern genetics? a. He showed how patterns of inheritance work (e.g. dominance vs. recessiveness) b. He demonstrated that species can change through natural or artificial selection c. He showed that DNA is the source of all genetic material d. He invented the butterfat test that enabled dairy farmers to create a genetic basis for improving their herds 70. What was the contribution of Mendel to modern genetics? a. He showed how patterns of inheritance work (e.g. dominance vs. recessiveness) b. He demonstrated that species can change through natural or artificial selection c. He showed that DNA is the source of all genetic material d. He invented the butterfat test that enabled dairy farmers to create a genetic basis for improving their herds 71. What was the contribution of Babcock to modern genetics? a. He showed how patterns of inheritance work (e.g. dominance vs. recessiveness) b. He demonstrated that species can change through natural or artificial selection c. He showed that DNA is the source of all genetic material d. He invented the butterfat test that enabled dairy farmers to create a genetic basis for improving their herds 72. What is a breed? a. A specific species that is domesticated b. A specific variety of a species of domesticated animal with similar traits and qualities. c. A term for the domesticated variety of the same species (the wild version would be a species) d. A family of different species with similar traits Copyright 2012 by Craig Kohn, Agricultural Sciences, Waterford WI. This source may be freely used and distributed provided the author is cited. 73. What two factors are most responsible for the emergence of different breeds of domesticated animals? a. Isolation and an understanding of Mendelian genetics b. An understanding of Mendelian genetics and different needs and environments of different locations c. Different needs and environments of different locations and isolation of those same places d. None of the above are responsible for the emergence of different breeds 74. Cold harsh weather would probably cause what kind of breed to arise? a. One that is very productive b. One that is very hardy and strong b. One that is very susceptible to illness d. One that is very efficient and ate little food 75. Which of the following best describes the Holstein breed? a. The highest quality milk b. The highest production of milk c. Dual purpose for meat and milk d. Hardy and strong 76. Which of the following best describes the Brown Swiss breed? a. The highest quality milk b. The highest production of milk c. Dual purpose for meat and milk d. Hardy and strong 77. Which of the following best describes the Jersey breed? a. The highest quality milk b. The highest production of milk c. Dual purpose for meat and milk d. Hardy and strong 78. Which of the following best describes the Milking Shorthorn breed? a. The highest quality milk b. The highest production of milk c. Dual purpose for meat and milk d. Hardy and strong 79. Which of the following is a measure of how likely offspring are to inherit the phenotypes of their parents? a. Genetic Modification b. Heritability c. Chromosome d. Nucleus 80. Milk yield has a heritability of 0.30. Days of productive life has a heritability of 0.13. Which one is most affected by the choice of bulls for mating? a. Milk Yield – it is a more heritable trait b. Days of productive life – it is a more heritable trait c. Both are strongly affected by heritability and should be equally considered when choosing a bull d. Neither are affected by heritability and the choice of bulls would not make much, if any difference 81. The higher the heritability the ___________ a trait is affected by a choice of bulls a. Less b. More 82. Genetic change can be made faster by… a. More accurate selection of bulls b. Bulls with a wide variety of inheritance c. A slow rate of reproduction Copyright 2012 by Craig Kohn, Agricultural Sciences, Waterford WI. This source may be freely used and distributed provided the author is cited. d. All of the above 83. Galton’s Law states that… a. Bulls with low heritability should not be bred b. Genetic change is slowed by traits that are not as heritable c. The stronger an individual is for a trait, the less likely that individual is to have offspring with traits that are expressed in the same way d. If you get up to get a soda, you also have to get Galton one Use the Sire Summaries below to answer the following questions. 84. Which bull would be the best choice for improving the milk fat percentage of your herd’s cows? a. R-E-W Buckeye ET b. Pursuit September Storm ET 85. Which bull would be the best choice for improving the milk protein percentage of your herd’s cows? a. R-E-W Buckeye ET b. Pursuit September Storm ET Copyright 2012 by Craig Kohn, Agricultural Sciences, Waterford WI. This source may be freely used and distributed provided the author is cited. 86. When genes from two different species are combined and introduced into a cell, this is known as… a. Biotechnology b. Recombinant DNA c. A DNA Vector d. Restriction Enzyme 87. The manipulation of the genetics of an organism to make useful products is known as… a. Biotechnology b. Recombinant DNA c. A DNA Vector d. Restriction Enzyme 88. The genome into which another organism’s DNA is inserted is known as… a. Biotechnology b. Recombinant DNA c. A DNA Vector d. Restriction Enzyme 89. A “sticky end” is… a. The kind of DNA needed in order to insert a vector b. The kind of enzyme needed to cut DNA c. The cutting of DNA by a restriction enzyme so that a single-stranded portion remains d. The ultimate demise of ants who fall into honey Use the cut DNA sequence below to answer the following question 90. A gene inserted into this genome would to begin with what sequence (i.e. what letters do the x’s below have to be in order for this gene to be inserted into this genome)? a. TTAA b. AATT c. CGAT d. GCTA X X X X 91. Which of the following would make the inserted gene a permanent part of its new genome? a. DNA Vector b. Restriction Enzyme c. DNA Ligase d. DNA Superglue 92. How did recombinant DNA help scientists sequence the human genome? a. Human genes were inserted into Taq polymerase, which made copies of the human DNA b. Human genes were spliced into E. coli bacteria, which reproduced the human DNA c. E. coli genes were spliced into human genomes, allowing us to read our own DNA d. The creation of Bt corn created the information needed to read human DNA 93. “Bt” in Bt corn stands for… a. “Bigger Tassels” b. Bacillus thuringiensis c. Borer-toxic 94. What is Bt corn? a. A type of corn that produces its own insecticide because of an inserted gene for toxin production b. A type of corn that is organically produced and cannot be sprayed with insecticides c. Any kind of corn that is genetically modified Copyright 2012 by Craig Kohn, Agricultural Sciences, Waterford WI. This source may be freely used and distributed provided the author is cited. Top Pair d. Corn that is especially vulnerable to the corn pest, Bacillus thuringiensis 95. How does Bt corn prevent being attacked by insects? a. It kills all insects that touch it b. When a specific inset, the corn borer, ingests it, it breaks down its intestinal tract c. It prevents insects from reproducing d. It does not prevent insect attacks 96. Which of the following is TRUE about Bt Corn and monarchs a. Bt corn has been shown to cause significant harm to monarch butterflies b. An early strain of Bt corn did initially cause harm to monarchs, but is not used anymore c. Bt corn has never had any impact on monarch butterflies 97. Golden Rice is rice that is genetically modified to produce its own ____________ a. Vitamin A b. Vitamin B c. β-carotene 98. What organism was used to introduce the genes for this compound into Golden Rice? a. Bacillus thuringiensis b. Taq polymerase c. E. coli d. Agrobacterium 99. What is a “Pharm Animal” a. An animal that is genetically modified to produce a medicine in its meat, eggs, or milk b. An animal that is genetically modified so that it does not need to be sprayed with insecticide c. An animal that is genetically modified so that it does not need to be sprayed with herbicide d. An animal that lives on a pharm 100. a. b. c. d. How could GMOs be used to help the environment? Microbes could be genetically engineered to clean up oil spills Microbes could be genetically engineered to clean up toxic waste Microbes could be genetically engineered to help us produce biofuel from cellulose All of the above are potential benefits of GMOs Copyright 2012 by Craig Kohn, Agricultural Sciences, Waterford WI. This source may be freely used and distributed provided the author is cited.