®
®
February 2014
Rev. No: 00
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and detailed Product Inserts
DR low resolution screening
101.103-48/12 -48u/12u
42V
Gel Documentation Form and Worksheet
DR low resolution screening
Lot No: 42V
Expiry Date: 2016-August-01
(101.103-48/12,-48u/12u)
Name:__________________
Test Date:_______________
Review Date:_______________
Sample ID:______________
Tested By:_______________
Reviewed By:_______________
DNA Conc.(ng/ul):_________
Gel Picture
PHOTO DOCUMENT
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
For In Vitro Diagnostic Use
Page 1 of 6
®
®
DR low resolution screening
101.103-48/12 -48u/12u
42V
February 2014
Rev. No: 00
ICB
1
2
3
4
5
6
205 200 200 210 120 75
Am pS
7
8
9
10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
515 430 430 430 430 430 430 430 430 515 430 430 430 430 430 430 430 430 430 430 430 430 430
235
230
85 100 210 170 90 175 100 85 215 195 175 100 110 110 160 215 175
220 210 210 175 235 215 135
260
170 110
250 185
225 240 140 140 150 240
NC
Well No.
Visit www.olerup-ssp.com for
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155 170 180
220
Well No.
Specific
Reaction
1
2
3
4
5
6
7
8
9
10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
‘ICB’ Internal Control Band,
‘AmpS’ Amplicon Size
Interpretation:_______________
Failed lanes:
_________
Comments:
___________________
Notes:
Product sizes are approximate. For detailed information, see the lot-specific Specificity Table and Interpretation Table.
This table is intended as a guide. For interpretation always use the Interpretation Table and/or Specificity Table.
HLA-specific PCR products shorter than 125 base pairs have a lower intensity and are less sharp than longer PCR
products
Individual alleles can give to rise to two differently sized specific PCR fragments in primer mix 1, 3, 5 to 11, 13, 14, 16 to
21.
Primer mixes 1, 8, 9, 15, 16, 20 and 22 have a tendency to giving rise to primer oligomer formation..
Primer mix 6 may give a lower yield of HLA-specific PCR product than the other DR low primer mixes.
Primer mix 3 may have tendency of unspecific amplification
Primer mix 19 has a tendency of primer oligomer formation and also has an intense primer cloud due to the high number of
primers present in the primer mix.
The DRB4*01:03:01:02N allele is amplified by the primer pairs in wells 18 and 22, whereas the DRB4*02:01N and
DRB4*03:01N null alleles are not amplified by these primer pairs.
Due to sharing of sequence motifs in codon 38, DRB3*01:14 will also be amplified in primer mixes 5, 6 and 17 in addition to
primer mix 21.
Due to sharing of sequence motifs, DRB3*02:27 is amplified by the primer pairs in well 15 in addition to primer mix 21.
Primer mix 24 contains a negative control, which will amplify more than 95% of HLA amplicons as well as amplicons
generated by a control primer pair. PCR product sizes range from 75 to 200 base pairs. The PCR product generated by
the control primer pair is 430 base pairs.
Olerup SSP AB
Franzengatan 5
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Sweden
For In Vitro Diagnostic Use
Page 2 of 6
®
®
February 2014
Rev. No: 00
DR
Well No.
2
3
4
5
6
5
6
5
6
7
8
DR1, Null, - 1
DR103/DR1
, Null
-
2
*01:42
1
*03:01:01:01-03:01:05, 03:01:07-03:01:08,
03:01:10-03:01:22, 03:04:01-03:06, 03:09,
03:11:01-03:16, 03:18-03:20, 03:22-03:23,
DR17, DR3,
03:25-03:26, 03:28, 03:30-03:31, 03:33-03:34,
Null, 03:36-03:37, 03:43-03:45, 03:47-03:48, 03:5003:52, 03:54-03:63, 03:66-03:68N, 03:70-03:73,
03:75, 03:77-03:86, 03:89, 03:91, 03:93
DR17, *03:01:06, 03:01:09, 03:46, 03:64, 03:92
*03:02:01-03:03, 03:27, 03:29, 03:38, 03:53,
DR18, DR3,
03:74, 03:88, 03:90
*03:07, 03:17, 03:21, 03:24, 03:32, 03:35, 03:39DR3, 03:41, 03:49
DR3, *03:08, 03:65
DR3
*03:10
*03:42, 03:69, 03:87
*03:76
*04:01:01-04:05:11, 04:05:13-04:61, 04:63DR4,
04:68, 04:70-04:72:02, 04:74-04:104, 04:106- DR4/DR3,
Null, 04:121, 04:123-04:145, 04:147-04:164
*04:62, 04:69, 04:73, 04:105:01-04:105:02,
DR4, 04:122, 04:146
DR7, Null, *07:01:01:01-07:01:07, 07:03-07:28
*08:01:01-08:02:04, 08:04:01-08:07, 08:11,
08:16-08:17, 08:22, 08:24, 08:26, 08:28, 08:39, DR8, 08:42-08:44, 08:50, 08:52, 08:54
*08:03:02-08:03:03, 08:10, 08:12-08:15, 08:1808:19, 08:23, 08:25, 08:27, 08:29-08:30:03,
DR8, 08:33-08:34, 08:36:01, 08:37-08:38, 08:4508:49, 08:51
DR8
*08:08
DR8
*08:09
*08:20, 13:18, 13:47, 13:55, 13:158, 13:164 3
*08:21
DR13, -
*08:31, 08:41, 11:67 3
*08:32, 08:53
*08:35, 08:36:02
*08:40
*09:01:02-09:01:05, 09:01:07-09:02:02, 09:0409:20, 09:22
*09:01:06, 09:03
*10:01:01-10:06
DR8/DR11, -
2
5
17
7
17
5
5
5
5
17
6
6
11
6
12
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
15
17
8
8
18
9
10
16
10
10
10
DR8
-
10
10
10
10
10
6
16
18
16
15 16 17
15 16
16
13
20
20
20
14
DR9, -
11
DR9
DR10, -
11
DR
13
19 20
20
20
19
12
*11:01:01-11:01:17, 11:01:20, 11:04:0111:04:11, 11:06:01-11:06:03, 11:09-11:10:02,
11:12:01-11:12:02, 11:15, 11:24, 11:27:0111:30, 11:32-11:33, 11:35, 11:37:01-11:39,
11:43-11:44, 11:46:01-11:47, 11:49:01-11:51,
11:54:01-11:54:02, 11:56, 11:58:01-11:58:02,
DR11, 11:60-11:62, 11:66, 11:72, 11:74:01-11:75,
11:77-11:78, 11:81, 11:84:01-11:84:03, 11:88,
11:90-11:92, 11:94-11:95, 11:97, 11:9911:102:02, 11:106, 11:108-11:117, 11:12011:121, 11:123, 11:126, 11:128-11:129, 11:13311:134, 11:137, 11:140-11:141, 11:144-11:147
Well No.
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Negative Control
*01:03, 01:39N
1
ser 2
1
DRB1 allele
*01:01:01-01:02:08, 01:04-01:38, 01:40N01:41, 01:43-01:60
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DR low resolution screening
101.103-48/12 -48u/12u
42V
Worksheet
13
1
2
3
4
5
6
7
8
15 16
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
For In Vitro Diagnostic Use
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®
February 2014
Rev. No: 00
Well No.
*11:01:18-11:01:19, 11:08:01-11:08:02, 11:1811:19:03, 11:42, 11:57, 11:130
*11:02:01-11:03, 11:11:01-11:11:02, 11:14:0111:14:02, 11:16, 11:20-11:21, 11:36, 11:4011:41, 11:48, 11:63, 11:65:01-11:65:02, 11:68,
11:70, 11:76, 11:85-11:86, 11:93, 11:118,
11:122, 11:124, 11:127, 11:138-11:139
*11:05
*11:07, 11:53, 11:103, 11:105, 11:107, 11:125
*11:13:01-11:13:02
*11:17, 11:52
DR
1
2
3
4
5
3
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
6
7
8
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
DR11, -
DR11,
DR11/DR13
,-
6
DR11
DR11, DR11
DR14,
DR11
DR11, DR11
DR11, DR11
DR11, -
5
11
w
15
13
15 16
13
13
13
16
17
19 20
19 20
13
13
13
13
13
12 13
13
13
13
13
7
6
6
15 16
20
15
20
20
16
15 16
16
15
18
20
15 16 17
14
10
10
9 10
14
16
14
14
14
16
6
15 16 17
6
17
6
15
17
15 16 17
15
17
16 17
DR13, -
15
DR13, DR13, -
DR
13
13
*11:22, 11:98, 11:104, 11:136, 11:143
*11:23:01-11:23:02, 11:25, 11:96, 11:148
*11:26, 11:34
*11:31, 11:45, 11:64, 11:119
*11:55
*11:59, 11:80, 11:83, 11:87, 11:135, 11:142
*11:69, 11:82
*11:73, 11:79, 11:131-11:132
*11:89
*11:149
*12:01:01-12:01:05, 12:03:02-12:03:03, 12:05DR12, Null,
12:12, 12:14, 12:17, 12:24N-12:25, 12:2812:30, 12:34-12:36, 12:38, 12:40-12:41
*12:02:01-12:02:05, 12:13, 12:15, 12:18-12:21,
DR12, Null,
12:23, 12:26-12:27, 12:31N-12:33, 12:37, 12:4212:43
DR12, *12:04, 12:39
*12:16:01-12:16:03
*12:22
*13:01:01-13:02:01, 13:02:03-13:02:09, 13:04,
13:08, 13:16, 13:20, 13:22-13:24, 13:28-13:29,
13:31-13:32, 13:34-13:36, 13:38-13:40, 13:48,
13:51-13:52, 13:54, 13:59, 13:61:01-13:61:02, DR13,
13:63-13:65, 13:68-13:70, 13:72-13:76, 13:78- DR11,
DR14, Null,
13:80, 13:83-13:84, 13:87, 13:91-13:93,
13:96:01-13:99, 13:102, 13:105-13:107,
13:109, 13:111-13:114, 13:117, 13:121, 13:12313:128, 13:130-13:131, 13:135, 13:138-13:143,
13:145, 13:147-13:149, 13:153, 13:155, 13:162
DR13
*13:02:02
*13:03:01-13:03:06, 13:10, 13:33:01-13:33:03,
13:37, 13:66:01-13:66:02, 13:81, 13:88-13:90, DR13, Null,
13:94:01-13:95, 13:101, 13:115, 13:120,
13:133, 13:137N, 13:151-13:152
*13:05:01-13:05:02, 13:07:01-13:07:02,
13:11:01-13:11:02, 13:14:01-13:14:03,
DR13,
DR13/DR11
13:21:01-13:21:02, 13:42, 13:46, 13:49, DR6, 13:50:02, 13:62, 13:100, 13:108, 13:132,
13:136, 13:150
*13:06, 13:12:01-13:12:03, 13:25, 13:30, 13:56, DR13,
DR11, DR6,
13:58, 13:60, 13:77, 13:82, 13:110, 13:118,
13:134, 13:157, 13:161, 13:163
DR13
*13:09
*13:13, 13:119, 13:154, 13:156, 14:84, 14:116,
14:144
*13:15, 13:19, 13:53, 13:57, 13:104
*13:17, 13:116
Well No.
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and detailed Product Inserts
DR low resolution screening
101.103-48/12 -48u/12u
42V
Worksheet
Negative Control
®
1
2
3
4
5
6
6
6
7
7
8
17
20
15 16 17
9
16
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
For In Vitro Diagnostic Use
Page 4 of 6
®
February 2014
Rev. No: 00
Well No.
*13:26:01-13:26:02
*13:27, 13:41, 13:71
*13:43, 13:159
*13:44, 13:86
*13:45
*13:67, 13:103, 13:160
*13:85
*13:122
*13:129
*13:144
*13:146
DR
1
2
3
4
5
DR14, DR13
DR13, DR13
DR13, DR14,
*14:01:01-14:01:02, 14:01:04, 14:04, 14:07:01- DR1404/DR
14,
14:07:02, 14:10, 14:54:01, 14:57
DR4/DR6, -
*14:01:03, 14:05:01-14:05:04, 14:14, 14:23:01,
14:23:03-14:23:04, 14:36, 14:43-14:45,
14:54:02, 14:56, 14:59, 14:64, 14:91, 14:96,
14:100, 14:103, 14:123, 14:127:01-14:127:02,
14:134
*14:02, 14:06:01-14:06:02, 14:09, 14:13, 14:17,
14:20, 14:29-14:30, 14:33, 14:41, 14:47-14:48,
14:51, 14:80, 14:83, 14:94, 14:106, 14:108,
14:121
*14:03:01-14:03:02, 14:12:01-14:12:02, 14:40,
14:63, 14:67, 14:77-14:78, 14:85, 14:102,
14:115, 14:135
*14:08, 14:23:02, 14:34, 14:72, 14:92N, 14:97,
14:126, 14:130, 14:133
*14:11
*14:15
*14:16
*14:18, 14:81
*14:19, 14:21, 14:109
*14:22, 14:105
*14:24
*14:25, 14:53, 14:128
*14:26, 14:28, 14:31, 14:32:03, 14:35, 14:3814:39, 14:55, 14:60-14:62, 14:70-14:71, 14:7514:76, 14:79, 14:86-14:88, 14:90, 14:99,
14:101, 14:104, 14:107, 14:110-14:114, 14:11714:118, 14:120, 14:122, 14:124-14:125,
14:129, 14:131, 14:138-14:140, 14:142-14:143
*14:27
*14:32:01-14:32:02
*14:37
*14:42, 14:136
*14:46
*14:49, 14:119
*14:50
*14:52
*14:58
*14:65
*14:68, 14:93
*14:69
*14:73
*14:74
Well No.
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DR low resolution screening
101.103-48/12 -48u/12u
42V
Worksheet
6
7
7
8
6
6
9 10 11 12 13 14 15
15
12
15
15
7
6
6
6
6
6
7
12
16 17 18 19 20 21 22 23 24
16 17
16 17
16
19 20
17
15 16
18
20
15 16
15
17
15
16 17
15 16 17
20
16 17
20
17 18 19 20
DR14, -
DR14, DR6,
-
7
DR1403/DR
14, DR6, -
7
DR14, Null,
DR14
DR8
DR6
DR14, DR14, DR14, DR14
DR6,
DR13/14, -
15
10
10
6
6
7
7
15
15 16
16
7
19 20
17
19
17
20
?
19 20
17
19 20
?
20
? 18 19 20
17
19 20
17
w
? 18 19 20
17
15 16 ? 18
DR14, -
20
? 18 19 20
DR14
DR14
DR14
DR14
DR14, DR14
DR14
DR14
DR6
DR14, -
DR
16
15 16
17
Negative Control
®
7
w
7
w
1
2
3
4
5
6
7
8
15 16 17
20
? 18 19 20
16 17 18
17
20
19
? 18 19 20
9
? 18 19 20
?
19
? 18
20
17
19 20
10
? 18 19 20
15
? 18
20
16 ? 18 19 20
15
? 18 19 20
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
For In Vitro Diagnostic Use
Page 5 of 6
®
DR low resolution screening
101.103-48/12 -48u/12u
42V
Worksheet
February 2014
Rev. No: 00
Well No.
*14:82
*14:89
*14:95
*14:98
*14:132
*14:137N
*14:141
*15:01:01:01-15:20, 15:22-15:24, 15:26, 15:2815:33, 15:35-15:65, 15:67-15:100
*15:21
*15:25
*15:27, 15:34, 15:66
*16:01:01-16:03, 16:05:01-16:05:02, 16:0716:17, 16:19-16:24
*16:04, 16:18
DRB3*01:01:02:01-01:15, DRB3*02:0102:26, DRB3*02:28-02:29N, DRB3*03:01:0103:03
DRB3*02:27
DRB4*01:01:01:01-01:03:01:01,
DRB4*01:03:01:03-01:08
DRB4*01:03:01:02N
DRB5*01:01:01-01:14, DRB5*02:02-02:06
Well No.
DR
1
2
3
4
5
Null
-
6
6
7
8
7
6
7
6
6
DR15, DR2,
Null, DR2
-
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9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
17 18 19 20
17
20
17
19 20
15
17
?
19 20
15
17 18 19 20
17
21
3
3
3
3
DR16, Null,
DR16, -
w
5
19
4
4
w
DR52, Null,
-
21
-
15
21
DR53, -
22
Null
DR51, Null,
-
DR
Negative Control
®
18
22
23
1
2
3
4
5
6
7
8
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
1Alleles
that have been deleted from or renamed in the official WHO HLA Nomenclature up to and including the last IMGT/HLA
database release can be retrieved from web page http://hla.alleles.org/alleles/deleted.html.
2The serological reactivity of all DRB alleles is not known. In this table we use the information in the HLA Dictionary 2004 on the
www.ebi.ac.uk/imgt/hla web site, the information available at the www.worldmarrow.org web site and the expert-assigned
serological grouping in Tissue Antigens (2009) 73:95-170.
3
The DRB1*08:20 and the DRB1*13:18, 13:47, 13:55, 13:158 and 13:164 alleles yield identical amplification patterns with the DR
low resolution screening primer set. These alleles can be separated by the respective high resolution primer sets.
The DRB1*08:31, 08:41 and DRB1*11:67 alleles yield identical amplification patterns with the DR low resolution screening primer
set. These alleles can be separated by the respective high resolution primer sets.
The DRB1*13:13, 13:119, 13:154 and 13:156 and the DRB1*14:84, 14:116 and 14:144 alleles yield identical amplification patterns
with the DR low resolution screening primer set. These alleles can be separated by the respective high resolution primer sets.
Olerup SSP AB
Franzengatan 5
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For In Vitro Diagnostic Use
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