Supplementary materials
Development of secretory cells and crystal cells in Eichhornia crassipes
ramet shoot apex
Guo Xin Xu, Chao Tan, Xiao Jing Wei, Xiao Yan Gao, Hui Qiong Zheng*
(Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological
Sciences, Chinese Academy of Sciences, Shanghai 200032, China)
Captions
To test if the abundance of crystal cells in water hyacinth shoot apexes is
related to the heavy metal tolerance, the amount of crystal cells in shoot
apexes of plants grown on the medium containing different concentrations of
Cd was investigated.
Figure S1 shows that there is almost no Cd to be
transported to the shoots and leaves and most of Cd retain in roots under low
concentration Cd (less than 10 mg/L) conditions, but content of Cd apparently
increased in shoot apexes and leaves under higher concentration Cd (more
than 10 mg/L) conditions. In contrast, the amount of crystal cells did not
significantly change in shoot apexes under both low and high concentration Cd
conditions (Fig. 2S A and B). In addition, Cd, even at high concentration,
couldn’t induce the formation of crystal cells under conditions of Ca deficiency
(Fig. 2S C). Thus, the mechanism of heavy metal tolerance in water hyacinth
might not depend on increasing the amount of crystals in shoot apical tissues,
but involve the sequestration of hazardous metals in the existing CaOx crystal
cells.
Supplementary Figures
Figure S1 Content of cadmium (Cd2+) in different tissues of water hyacinth
grown in the medium containing different concentrations of Cadmium
determined by inductively coupled plasma mass spectroscopy (ICP-MS).
Figure S2 Distribution densities of secretory cells and crystal cells in shoot
apexes of water hyacinth grown in media with or without Calcium and
Cadmium. A, Control. B, Plants were grown in the normal control medium
adding 10 mg/L CdCl2. C, Plants were grown in the control medium without
Calcium, but adding 10 mg/L CdCl2. Cr, Crystal cell, SC, Sectory cell, X, xylem.
Bars, 0.05 mm.