Q1. (a) A food contains protein. Describe, in as much detail as you can, what happens to this protein after the food is swallowed. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (4) (b) The table shows the activity of lipase on fat in three different conditions. CONDITION UNITS OF LIPASE ACTIVITY PER MINUTE Lipase + acid solution 3.3 Lipase + weak alkaline solution 15.3 Lipase + bile 14.5 Explain, as fully as you can, the results shown in the table. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (3) (Total 7 marks) Page 1 Q2. The graph shows the effect of temperature on photosynthesis. (a) Between which temperatures is the rate of photosynthesis fastest? ............................... and .......................... °C (1) (b) Suggest why the rate of photosynthesis stays the same between these two temperatures. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (2) (c) A greenhouse owner wants to grow lettuces as quickly and cheaply as possible in winter. At what temperature should he keep his greenhouse in order to grow the lettuces as quickly and cheaply as possible? Page 2 .......................... °C Explain your answer. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (3) (Total 6 marks) Q3. The drawing shows a white blood cell ingesting a bacterium. Label the parts of the white blood cell. (Total 3 marks) Q4. Green plants make food in their leaves. (a) From where do the leaves get the energy that they need to make food? Page 3 ..................................................................................................................................... (1) (b) The graph shows the effect of temperature on the rate of photosynthesis. (i) Between which temperatures is the rate of photosynthesis fastest? .............................. and .............................. °C (1) (ii) Suggest why the rate of photosynthesis stays the same between these two temperatures. ............................................................................................................................ ............................................................................................................................ ............................................................................................................................ ............................................................................................................................ (2) (iii) A greenhouse owner wants to grow lettuces as quickly and cheaply as possible in winter. At what temperature should he keep his greenhouse in order to grow the Page 4 lettuces as quickly and cheaply as possible? .............................. °C Explain your answer. ............................................................................................................................ ............................................................................................................................ ............................................................................................................................ ............................................................................................................................ (3) (Total 7 marks) Q5. The table shows the amounts of carbohydrate, fat and protein in 100 g portions of five foods,A - E. MASS IN 100 g PORTION (g) FOOD CARBOHYDRATE FAT PROTEIN A 0 1 20 B 50 2 8 C 0 82 0 D 12 0 1 E 20 0 2 (a) A person eats 50 g of food E. How much carbohydrate would the person eat? ................ g (1) (b) Describe, in as much detail as you can, what happens to the protein after food A is swallowed. Page 5 ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (4) (Total 5 marks) Q6. The diagrams show four ways in which human twins may be formed. Page 6 Which diagram, A, B, C or D, shows the process which will produce genetically identical twin boys? ...................................................... Explain the reason for your choice. ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... Page 7 ............................................................................................................................................... (Total 3 marks) Q7. The drawing shows some of the fossils found in the layers of rock in two cliffs. The two cliffs are on opposite sides of a large valley. Geologists think that the valley has been carved out by rivers, and that the order of rock layers has not changed. (a) (i) Which of the rock layers, A, B, C or D, is the oldest? ................................... (1) (ii) Give the letters of two layers of rock on opposite sides of the valley that are the same age. .............................. and .............................. (1) (b) How do fossils provide evidence for the theory of evolution? ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... Page 8 ..................................................................................................................................... (2) (Total 4 marks) Q8. The drawing shows a white blood cell ingesting a bacterium. (i) Use words from the list to label the parts of the white blood cell. cell membrane cell wall cytoplasm nucleus vacuole (3) (ii) The scale shows that the white blood cell is 10 micrometres long. How long is the bacterium? Show your working. ................... micrometres (2) (Total 5 marks) Page 9 Q9. Most people have a gene which produces a protein called CFTR that enables the cells lining the lungs to work efficiently. In people suffering from cystic fibrosis this gene is faulty; it produces a protein which lacks just one of the 1480 amino acids found in CFTR. (i) Name the molecule which carries the genetic information for producing proteins such as CFTR. ..................................................................................................................................... (1) (ii) Explain how this molecule is responsible for the structure of proteins such as CFTR. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (3) (Total 4 marks) Q10. Read the extract. Super-bug may hit the price of coffee The coffee bean borer, a pest of the coffee crop, can be controlled by the pesticide endosulphan However, strains of the insect that are up to 100 times more resistant to the pesticide have emerged on the South Pacific island of New Caledonia. For full resistance to be passed on to an offspring two copies of the new resistance allele 5 should be inherited, one from each parent. There is much inbreeding with brother-sister matings happening in every generation, so it takes only a few generations before all the Page 10 descendants of a single resistant female have inherited two copies of the resistance allele. If this resistance spreads from New Caledonia, it will mean the loss of a major control 10 method. This will present a serious threat to the international coffee industry. (a) Suggest how the allele for resistance to endosulfan may have arisen. ..................................................................................................................................... ..................................................................................................................................... (1) (b) (i) How would you expect the proportion of normal coffee bean borers on New Caledonia to change over the next few years? ........................................................................................................................... (ii) Explain why this change will take place. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (3) (c) Explain why “it takes only a few generations before all the descendants of a single resistant female have inherited two copies of the resistance allele.” (lines 6-8) ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... Page 11 ..................................................................................................................................... ..................................................................................................................................... (3) (Total 7 marks) Q11. In an investigation four groups of athletes were studied. The maximum rate of oxygen consumption for each athlete was measured and the mean for each group was calculated. The athletes then ran 10 mile races and the mean of the best times was calculated for each group. The results are shown in the table below. (i) What is the relationship between maximum rate of oxygen consumption and time for a 10 mile race? ..................................................................................................................................... ..................................................................................................................................... (1) (ii) Suggest an explanation for this relationship. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... Page 12 ..................................................................................................................................... (3) (Total 4 marks) Q12. Coat colour in rabbits is controlled by one pair of genes. The allele for black coat (N) is dominant to the allele for brown coat (n). The drawing shows the result of crossing a black-coated rabbit with a brown-coated rabbit. Complete the genetic diagram to show how the young rabbits inherited their coat colour. Use the symbols N and n for the alleles. The alleles of the brown parent have been inserted for you. Page 13 (Total 3 marks) Q13. A student breathed out into an empty breathing bag five times. Page 14 After breathing out five times the volume of air in the bag was measured. The volume was 3000 cm3. (a) Complete the following sentences. The air the student breathed in would contain more ........................................ than the air the student breathed out. The air the student breathed out would contain more ...................................... than the air the student breathed in. (2) (b) The student then did some exercise for two minutes. The volume breathed out in five breaths was again measured. This time there was 9000 cm3 of air in the bag. What does this tell you about the effect of exercise on breathing? ..................................................................................................................................... ..................................................................................................................................... (1) (c) (i) Name the chemical process that releases energy when it takes place in the cells of the body. ................................................ (1) (ii) Name the substances produced by this process. .......................................................... and .......................................................... (2) (iii) Explain as fully as you can why this process has to take place more rapidly during exercise. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) Page 15 (Total 8 marks) Q14. The drawing is an artist’s impression of some extinct plants. (a) Use only information from page 31 of the Data Book to help you to answer both parts (i) and (ii) of the question. These plants lived in forests. They died out at the end of the Carboniferous period. (i) How long ago did these plants become extinct? .................................... million years. (1) (ii) Suggest one reason why these plants became extinct. ........................................................................................................................... ........................................................................................................................... (1) Explain your answer. Page 16 ........................................................................................................................... ........................................................................................................................... (1) (b) We know about these extinct plants from their fossils. Describe how parts of plants become fossils. ..................................................................................................................................... ..................................................................................................................................... (1) (Total 4 marks) Q15. Wild turkeys have black feathers. Until about 30 years ago turkeys reared for meat also had black feathers like this. However, a recessive gene which produced entirely white feathers appeared, and turkey farmers changed to breeding white-feathered birds. Page 17 Supermarkets preferred white-feathered birds, because small pieces of feather left in the skin after plucking were not visible as dark patches. Customers wanted unblemished oven-ready birds. Now, however, there is a demand again for birds with black feathers which can be marketed as ‘traditional’ farm-produced turkeys. (a) Feather colour is controlled by one pair of genes. (i) Suggest suitable symbols for each of the two alleles of this pair of genes. Black feathers ........................ White feathers .................................. (1) (ii) What alleles for feather colour would a white turkey have? .......................... (1) (b) Explain carefully why ‘traditional’ black-feathered turkeys could not be bred from a flock of white-feathered birds. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (2) (Total 4 marks) Q16. The graph shows the mean light intensity at different times of the year in an oak wood. Page 18 (a) (i) In which month would you expect the rate of photosynthesis in the oak trees to be greatest? ......................................................................... (1) (ii) There are plants living on the ground in the wood. In which month would you expect their rate of growth to be fastest? ........................................................................................................................... Explain your answer. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (3) (b) Name two factors, other than light intensity, that would affect the rate of photosynthesis in the oak trees. 1 .................................................................................................................................. 2 .................................................................................................................................. (2) (Total 6 marks) Page 19 Q17. The picture shows the fossil remains of a bird. (a) Look carefully at the picture. Some parts of the bird were fossilised. (i) What were these parts made of? ........................................................................................................................... (1) (ii) Explain why these parts have been preserved. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) (iii) How can you tell that this fossil was a bird? (You might find information from Page 20 page 20 of the Data Book helpful.) ........................................................................................................................... (1) (b) This bird lived about 140 million years ago. This type of bird is now extinct. (i) What does ‘extinct’ mean? ........................................................................................................................... (1) (ii) Suggest one reason why this bird became extinct. ........................................................................................................................... ........................................................................................................................... (1) (iii) Name one other example of an animal that became extinct many millions of years ago. ........................................................................................................................... (1) (Total 7 marks) Q18. A student’s hobby was breeding pet mice. Three of the pet mice were called Stan, Tom and Sharon. Stan and Tom had black fur. Sharon had white fur. The colour of the fur is controlled by a single gene which has two alleles B and b. (a) The student first crossed Stan with Sharon. The results are shown on the diagram. Page 21 Explain why the baby mice produced by crossing Stan and Sharon all had black fur. You may use a genetic diagram if you wish. (3) (b) The student then crossed Tom with Sharon. The results are shown on the diagram. Page 22 When Tom was crossed with Sharon, some of the baby mice had black fur and some white. Explain why. You may use a genetic diagram if you wish. (3) (Total 6 marks) Q19. (a) Complete the equation for photosynthesis. (2) (b) The diagram below is printed in a plant care manual. Use information from the diagram to answer the following questions. Page 23 (i) Name one type of plant which could live on the floor of a dense forest in the middle of summer. .......................................................................................................................... (1) (ii) Explain the reason for your answer to (i) above. .......................................................................................................................... .......................................................................................................................... (1) (iii) The drawing shows one type of plant with variegated leaves. The manual says that these plants need direct sunlight. Suggest and explain why this plant needs ‘some direct sunlight’ in order to develop satisfactorily. .......................................................................................................................... .......................................................................................................................... (2) (iv) The drawing shows a cactus. Page 24 Suggest and explain why cacti can only develop satisfactorily if they receive full sunlight. .......................................................................................................................... .......................................................................................................................... .......................................................................................................................... .......................................................................................................................... (2) (Total 8 marks) ## The following is adapted from an article which appeared in January 1994 in the Sunday Times. A woman gives birth at 59 Designer babies are just the first step in science’s ability to create a super race. Test-tube baby techniques give doctors an unprecedented degree of control over the offspring produced. When eggs and sperms are donated they seek to match the charactaristics of the donor whose role has been replaced. As more is learned about the genetic contribution to charactaristics such as intelligence, beauty or strength, this capacity to mix-and-match will increase. Technological change frightens us when it is outside our control and when we fear how it might be exploited by others. People wonder where the potential Brave New World of “retirement births” and ‘designer’ babies will ultimately lead us: cloning, genetic grading and even the introduction of animal genes into humans. Unthinkable, perhaps, but entirely possible. The techniques are there – in fact they have been for years. But who on earth would want to use Page 25 them, apart from some power mad politician? Use information from the article and any of your own knowledge to discuss the arguments for and against allowing doctors to experiment with human fertilisation techniques. You will not receive marks for simply copying extracts from the article. (Total 8 marks) Q21. Marathon runners are recommended to have a high carbohydrate diet prior to a race. Three athletes tried out three dietary regimes prior to a marathon race. These three dietry regimes were as follows. Athlete A Up to 7 days before the race - Normal mixed diet 7 days before the race - Prolonged extreme physical activity 6-3 days before the race carbohydrate - Protein and fat diet; no 2 and 1 days before the race - Large carbohydrate intake Athlete B Up to 5 days before race - Normal mixed diet 5 days before the race - Prolonged extreme physical activity 4-1 days before the race - Large carbohydrate intake - Normal mixed diet - Large carbohydrate intake Athlete C Up to 4 days before the race 4-1 days before the race Page 26 The graph below shows the effect of each of these dietary regimes on glycogen levels in the athletes’ muscles (a) (i) What is the immediate effect of extreme physical activity on the glycogen content of muscles? ........................................................................................................................... ........................................................................................................................... (1) (ii) Describe how this effect occurs. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (3) (b) (i) Evaluate the three regimes as preparation for a marathon race. ........................................................................................................................... Page 27 ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (3) (ii) Suggest a possible explanation for the different effects of the three regimes. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) (Total 9 marks) Q22. The diagram below shows a food web for some of the organisms which live in a pond. (a) (i) Name one secondary consumer in this food web. ........................................................................................................................... (1) Page 28 (ii) The algae are small green plants. Give three conditions needed by green plants to produce sugars. 1 ........................................................................................................................ 2 ........................................................................................................................ 3 ........................................................................................................................ (3) (b) This is a pyramid of biomass for the organisms in the aquarium. Some of the biomass of the producers is not transferred to the tertiary consumers. Explain, as fully as you can, what happens to this biomass. (6) (Total 10 marks) Q23. The graph below shows the effect of a high carbohydrate diet on the stored carbohydrate in the muscles. Page 29 Diet A Diet B Diet C – – – High carbohydrate diet, started after several days of eating a diet without carbohydrate. High carbohydrate diet, started after normal mixed diet. Normal mixed diet. What advice would you give the athlete about the best diet preparation for a long race? Explain why you would give this advice. Diet ...................................................................... Explanation ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... (Total 2 marks) Page 30 Q24. (a) Put a tick ( ) in the correct boxes in the table below to show which of the parts given are present in the cells and organisms listed. CYTOPLASM NUCLEUS CELL WALL GENES Leaf mesophyll cell Sperm (2) (b) (i) What is the main job of a leaf mesophyll cell? ........................................................................................................................... ........................................................................................................................... (1) (ii) Explain one way in which the structure of the leaf mesophyll cell helps it to carry out its job. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) (Total 5 marks) Q25. (a) Put a tick ( ) in the correct boxes in the table below to show which of the parts given are present in the cells and organisms listed. CYTOPLASM Leaf mesophyll cell Sperm Page 31 NUCLEUS CELL WALL GENES (2) (b) (i) What is the main job of a leaf mesophyll cell? .................................................................................................................. .................................................................................................................. (1) (ii) Explain one way in which the structure of the leaf mesophyll cell helps it to carry out its job. .................................................................................................................. .................................................................................................................. .................................................................................................................. .................................................................................................................. (2) (Total 5 marks) Q26. This is a diagram of a belt transect showing the major types of plants growing on the bottom of a lake. (a) Suggest, and explain, two reasons why a much smaller population of Nitella plants is found amongst the Potamogeton plants than further down in the lake. 1. ................................................................................................................................ Page 32 .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... 2. ................................................................................................................................ .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... (4) (b) Describe how you would use the belt transect technique to measure the abundance and distribution of plants which live on the bottom of a shallow lake. .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... (3) (Total 7 marks) Q27. Flightless birds called Rails once inhabited 20 islands in the Pacific Ocean. During the last two centuries they have disappeared from 15 of these islands. The Aldabra Rail, shown below, is one of the few survivors. The island which it lives on is very remote. Page 33 Suggest three reasons why Rails have disappeared from 15 of the 20 islands they once inhabited. 1. .......................................................................................................................................... ............................................................................................................................................... 2. .......................................................................................................................................... ............................................................................................................................................... 3. .......................................................................................................................................... ............................................................................................................................................... (Total 3 marks) Q28. A plant with variegated (two-coloured) leaves was left in sunlight for several hours. Pieces of one of its leaves were then detached (removed) and tested for sugar. The diagram below shows the results. Explain, as fully as you can, why the yellow region of the leaf had not produced sugar. ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... Page 34 ............................................................................................................................................... ............................................................................................................................................... (Total 2 marks) Q29. The drawings below show a mammoth, an extinct relation of the elephant which lived in arctic regions, and a modern elephant which lives in tropical areas. The mammoth, which was very hairy, and the elephant, are both thought to have evolved from a scantily haired ancestor. Explain, as fully as you can, how the mammoth evolved from the common ancestor. ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... (Total 5 marks) ## Page 35 The passage below is an extract from an article in The Independent newspaper. This Mouthwash could mean a lot to your Children A SIMPLE TEST SCREENS WOULD-BE PARENTS FOR THE DEFECTIVE GENE THAT CAUSES CYSTIC FIBROSIS It is not every week that a university professor tells you to spit in front of him. But Bob Williamson, professor of biochemistry and molecular genetics asked me to do just that. In future, some people may choose their marriage partners on the basis of the contents of their spit. The professor handed me a sample bottle containing sterile salt solution. I rinsed my mouth out and spat back into the tube. Incredible though it may sound, the mouthwash now contained enough cells from the inner lining of my cheek for an assistant to dissect my DNA and check whether I carry any of the mutations responsible for cystic fibrosis. Professor Williamson’s team can locate genetic markers, distinctive segments of DNA, that are inherited along with the mutant genes in people affected with cistic fibrosis. About 16 000 people who bought The Independent this morning unwittingly carry a cystic fibrosis gene. The statistics indicate that 23 of the staff of this newspaper are unknowing carriers. Carriers are normal healthy individuals who do not have the disease. (a) Describe, as fully as you can, where genes are located inside cheek cells. ..................................................................................................................................... ..................................................................................................................................... (2) (b) The gene for cystic fibrosis has two forms called alleles. Only the recessive allele causes cystic fibrosis. Explain how two healthy carriers of the cystic fibrosis allele could produce a child with the disease. Use the symbol A for the normal allele of the gene and a for the allele which produces the disease. You may use a diagram if you wish. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... Page 36 (4) (d) In the test used to identify the ‘genetic markers’, DNA is extracted from the cheek cells. The DNA molecules are then made to produce hundreds of millions of copies of themselves. (i) Explain, as fully as you can, how the structure of DNA molecules allows them to replicate themselves. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (6) (ii) Explain how DNA controls the structure of proteins. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (3) (Total 15 marks) Page 37 Q31. (a) During respiration, sugar is oxidised to release energy. Complete the equation for respiration. Sugar + ............................. = ............................. + ............................. + energy (3) (b) The photograph below shows an athlete using an exercise machine. The machine can be adjusted to vary the rate at which the athlete is required to work. The athlete’s heart rate and breathing rate were measured at different work rates. The table below shows the results which were obtained. WORK RATE (J/s) HEART RATE (beats/min.) BREATHING RATE (breaths/min.) 0 86 9.6 60 106 10.0 80 112 10.4 100 122 10.4 120 135 11.4 140 143 14.5 160 156 15.8 200 174 30.5 Page 38 Plot the data on the graph paper below. (3) (c) Explain, as fully as you can, the advantages to the body in the change in breathing and heart rates. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... Page 39 ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (6) (d) This increase in the rate of heart-beat is a response to a stimulus. For this response suggest: (i) the stimulus; .......................................................................................... (ii) the co-ordinator; .......................................................................................... (iii) the effector. .......................................................................................... (3) (Total 15 marks) Q32. Person A and Person B measured their pulse rates over a period of five minutes. For one minute of this time they exercised by stepping on and off a box. At other times they sat still. The graph shows the results for Person A. Page 40 (i) What does the graph tell you about the changes in the pulse rate of Person A within the five minute period? ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (3) (ii) What was the pulse rate of Person A at the end of the five minute period? ..................................................................................................................................... (1) (iii) The table shows the results obtained for Person B. Time in minutes Pulse rate per minute 0 68 Page 41 1 68 2 110 3 96 4 80 5 68 Plot these results on the graph. (2) (Total 6 marks) Q33. In humans, the sex chromosomes X and Y determine whether the baby will be male or female (its gender). (a) (i) Draw a genetic diagram to show how gender is inherited. The male has XY chromosomes and the female has XX. (2) (ii) What is the likelihood of obtaining a male child? ......................................................................................................................... (1) (b) In the 16th century Henry VIII was the King of England. He blamed some of his wives for giving birth to daughters instead of sons. With our present day knowledge of genetics this mistake could not be made today. Explain why Henry VIII was wrong. .................................................................................................................................... .................................................................................................................................... Page 42 .................................................................................................................................... (2) (Total 5 marks) Q34. The drawing shows an animal cell, seen at a very high magnification using an electron microscope. (a) (i) Label a mitochondrion [plural = mitochondria]. (1) (ii) What happens in the mitochondria? .......................................................................................................................... (1) (b) (i) Name and label the structure where you would find chromosomes. (1) Page 43 (ii) What are chromosomes made of? .......................................................................................................................... (1) (c) What controls the rate of chemical reactions in the cytoplasm? .................................................................................................................................... (1) (Total 5 marks) Q35. Photosynthesis takes place the leaves of green plants. (a) Write a balanced chemical equation for the formation of glucose by photosynthesis. .................................................................................................................................... (3) (b) Describe two ways that the rate of photosynthesis can be decreased without lowering the temperature. .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... (2) (c) Some students decided to investigate the effect of temperature on the rate of photosynthesis in pond weed. They set up the apparatus and altered the temperature using ice and hot water. The counted the number of bubbles given off in a minute at different temperatures. They obtained the following results. Page 44 (i) Plot the points on the graph. (3) (ii) Use your graph to predict the number of bubbles per minute at 25 °C. .......................................................................................................................... (1) (iii) Suggest a reason why the rate of photosynthesis seems to decrease in this pondweed after 40 °C. Page 45 .......................................................................................................................... .......................................................................................................................... (1) (Total 10 marks) Q36. (a) The diagram shows a normal body cell which has six chromosomes. (i) Complete the diagram below to show one cell produced from this cell by mitosis. (3) (ii) Complete the diagram below to show one cell produced from the original cell by meiosis. Page 46 (2) (b) Thalassaemia is a blood disease. It is determined by a single recessive allele. A person with one recessive allele does not get the disease but does act as a carrier. People with this pair of recessive alleles can become ill. (i) Draw a genetic diagram to show the inheritance of' this disease if both parents are heterozygous. [Use the symbols T = dominant allele and t = recessive allele] (3) (ii) What are the chances of a baby inheriting the disease? .......................................................................................................................... (1) (iii) What are the chances of a baby being a carrier if both parents are heterozygous? .......................................................................................................................... (1) (Total 10 marks) Page 47 Q37. The diagram shows how a leaf of a green plant makes glucose. (a) Use words from the box to complete the labels on the diagram. You may use each word once or not at all. carbon dioxide chlorophyll light oxygen glucose heat water (5) (b) (i) Compete the following sentence. Glucose in food is a type of ................... . When we eat it, it gives us energy. (1) Page 48 (ii) The plant turns some of the glucose into starch. Why is starch useful to the plant? .......................................................................................................................... .......................................................................................................................... (1) (iii) What does the plant do with the rest of the glucose? .......................................................................................................................... (1) (c) (i) What is the name of the process outlined in the diagram? .......................................................................................................................... (1) (ii) Give one way that leaves are adapted to do this process. .......................................................................................................................... (1) (Total 10 marks) Q38. (a) Photosynthesis is a process that takes place in green plants. (i) What type of energy is needed for this process? .......................................................................................................................... (1) (ii) What substance in the plant absorbs this energy? .......................................................................................................................... (1) Page 49 (iii) In which part of the plant cell does photosynthesis take place? .......................................................................................................................... (1) (iv) Write a balanced chemical equation for photosynthesis. ......................................................... → .......................................................... (3) (b) Describe two ways you could speed up photosynthesis. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (2) (c) The diagram shows the outline of a cross-section of a leaf. Name cells 1 and 2 and describe how they are involved in photosynthesis. ................................................................................... ................................................................................... ................................................................................... ................................................................................... ................................................................................... ................................................................................... ................................................................................... ................................................................................... (4) (Total 12 marks) Page 50 Q39. The diagram shows a human sperm. Inside the tail of the sperm is a filament mechanism that causes the side to side movement of the tail, which moves the sperm. (a) Describe the function of the mitochondria and suggest a reason why they are arranged around the filament near the tail of the sperm. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (3) (b) Explain the significance of the nucleus in determining the characteristics of the offspring. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (2) (Total 5 marks) ## (a) Complete the table to give one site where digestive substances are made. Digestive substance One site of production Page 51 bile amylase lipase protease (4) (b) Describe two ways that the mouth can break down starchy foods. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (2) (c) Describe how the liver helps to digest fats. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (2) (Total 8 marks) Q41. The chromosomes for determining the gender or sex of a person are labelled X and Y. Page 52 (a) Complete the Punnett Square to show the genotype of parent 2 and of the four offspring. (3) (b) Which parent is the mother? ..................................................................................................................................... (1) (c) What are the chances of getting a baby boy? ..................................................................................................................................... (1) (Total 5 marks) Q42. (a) Plants make their own food by photosynthesis. Page 53 Use the following words to fill in the gaps. You can use each word once or not at all. carbon chlorophyll oxygen cytoplasm sound starch light nitrogen water During photosynthesis .............................................. dioxide and ............................. are converted into glucose and ........................................ . The energy needed to do this is ......................................... energy which is trapped by a green pigment called ......................................... . The plant can change the glucose into ..................................... which is insoluble so it can be stored. (6) (b) Which part of a plant is adapted for photosynthesis? .................................................................................................................................... (1) (c) How do the two raw materials for photosynthesis get into the plant? 1. ............................................................................................................................... .................................................................................................................................... Page 54 2. ................................................................................................................................ .................................................................................................................................... (2) (d) Describe one way you could speed up photosynthesis. .................................................................................................................................... ..................................................................................................................................... (1) (Total 10 marks) Q43. Meiosis and mitosis are different types of division in human cells. Compare the two processes by referring to where each takes place and the kind of products that are made. ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... (Total 6 marks) Page 55 Q44. In the 1850s an Austrian monk, called Gregor Mendel, carried out a series of investigations on heredity. (i) What plants did he use for his investigations? .................................................................................................................................... (1) (ii) In his work he assumed that one gene controlled one characteristic. He started his investigations with pure breeding parents. Use a genetic diagram to show how he explained the following result. (4) (Total 5 marks) Q45. (a) The volume of blood pumped out of the left ventricle at each beat was measured for a person during six different activities. These activities showed an increasing energy demand, with rest requiring the least energy and rowing a boat the most. The results of these measurements are shown on the bar chart. Page 56 (i) The pulse rate was also measured for the person during the same activities. The table shows the results that were obtained. Activity Pulse rate in beats per minute Rest 70 Writing 85 Cleaning the floor 100 Wallpapering 120 Walking fast 132 Rowing a boat 153 On the graph paper below draw a bar chart of the results obtained for the measurements of the pulse rate. Page 57 (2) (ii) Undertaking activities with increasing energy demand has an effect on the volume of blood pumped from the left ventricle (per beat) and on the pulse rate. What do the bar charts show these effects to be? Use only information shown in the bar charts in your answer. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) (b) The pulse rate changed when the activity changed. Explain the reason for this. .................................................................................................................................... .................................................................................................................................... .................................................................................................................................... (2) (Total 6 marks) Page 58 Q46. (a) Complete the following sentences. Green plants produce their own food by a process called photosynthesis. In this process the raw materials are ...................................................... and carbon dioxide. Glucose and ............................................................... are produced. .................................................... energy is absorbed by the green substance called .................................................... . (4) (b) Name two things that can happen in the plant to the glucose produced in photosynthesis. 1. .............................................................................................................................. 2. ............................................................................................................................... (2) (c) Plants need mineral salts. (i) Through which part do mineral salts get into the plant? ........................................................................................................................... (1) (ii) Explain why water is important in this process. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) Some students set up water cultures to find out how plants use nitrates. They had two sets of nutrient solutions. A full solution provided the plant with all the required nutrients. Page 59 The results table shows the average mass of the seedlings after 28 days of growth. (d) (i) Give a conclusion you could make from these results. ........................................................................................................................... ........................................................................................................................... (1) (ii) Calculate the difference in average mass caused by the addition of nitrates to the culture solution. ........................................................................................................................... (1) (iii) What are nitrates used for in the seedling? ........................................................................................................................... (1) (iv) Some factors need to be controlled to keep this test fair. Name two of them. 1. ..................................................................................................................... 2. .................................................................................................................... (2) Page 60 (v) Suggest one way you could improve the experiment. ........................................................................................................................... (1) (Total 15 marks) Q47. (a) Mice with black fur can have the genotype BB or Bb, whilst mice with brown fur have the genotype bb. (i) Use a genetic diagram to show what fur colours you would predict in the F1 offspring produced by two mice who are both Bb. (3) (ii) Why might your prediction of fur colour in the F1 generation not be proved right? ........................................................................................................................... ........................................................................................................................... (1) (b) Using the example in part (a) to help: (i) describe the difference between dominant and recessive alleles; ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... Page 61 ........................................................................................................................... (2) (ii) describe the difference between alleles and genes; ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) (iii) describe the difference between homozygous and heterozygous chromosomes. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) (Total 10 marks) Q48. The black pigment in human skin and eyes is called melanin. Production of melanin is controlled by a single pair of genes. A person who is homozygous for a recessive allele of the gene has no melanin and is said to be albino. (a) A man is albino. His wife is heterozygous for the melanin-producing allele. (i) The fertilised egg cell produced by the couple divides to form two cells. Name the process of cell division involved. .......................................................................................................................... (1) (ii) How many albino genes would there be in each of these two cells? Page 62 .......................................................................................................................... Explain you answer. .......................................................................................................................... .......................................................................................................................... .......................................................................................................................... (3) (b) (i) Albino people are more likely than people with melanin to suffer mutations that cause cancer in their skin. Suggest why albino people have an increased chance of mutation in their skin cells. .......................................................................................................................... .......................................................................................................................... (1) (ii) Sometimes, mutation in skin cells leads to cancers in other organs, such as the liver. Explain how. .......................................................................................................................... .......................................................................................................................... .......................................................................................................................... .......................................................................................................................... (2) (Total 7 marks) Q49. The graph shows the effect of increasing the carbon dioxide content of the inhaled air on: • the number of breaths per minute; • the total volume of air breathed per minute. Page 63 (i) Describe the effect of increasing the percentage of carbon dioxide in the inhaled air on the total volume of air breathed. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (2) (ii) Suggest why the total volume of inhaled air is not directly proportional to the number of breaths per minute. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (2) (Total 4 marks) Page 64 Q50. (a) Use words from the list to complete the sentences. alleles chromosomes gametes genes mutations The nucleus of a cell contains thread-like structures called ..................................... . The characteristics of a person are controlled by ..................................... which may exist in different forms called ..................................... . (3) (b) The drawing shows some of the stages of reproduction in horses. (i) Name this type of reproduction ....................................................................... (1) (ii) Name the type of cell labelled A ..................................................................... (1) Page 65 (c) When the foal grows up it will look similar to its parents but it will not be identical to either parent. (i) Explain why it will look similar to its parents. ........................................................................................................................... ........................................................................................................................... (1) (ii) Explain why it will not be identical to either of its parents. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) (Total 8 marks) Q51. The drawing shows part of a root hair cell. (a) Use words from the list to label the parts of the root hair cell. cell membrane cell wall cytoplasm Page 66 nucleus vacuole (4) (b) The diagram shows four ways in which molecules may move into and out of a cell. The dots show the concentration of molecules. The cell is respiring aerobically. Which arrow, A, B, C or D represents: (i) movement of oxygen molecules; (ii) movement of carbon dioxide molecules? .................... .................... (2) (c) Name the process by which these gases move into and out of the cell. ..................................................................................................................................... (1) (Total 7 marks) Q52. This couple has just found out that the woman is pregnant. They wonder whether the child will be a boy or a girl. Page 67 (a) Fill in the boxes to show the sex chromosomes of the woman and the man. (2) (b) The couple already has one girl. What is the chance that the new baby will be another girl? ..................................................................................................................................... Explain the reason for your answer. You may use a genetic diagram if you wish. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... Page 68 ..................................................................................................................................... ..................................................................................................................................... (3) (Total 5 marks) Q53. The diagram shows a plant leaf during photosynthesis. (a) Name: (i) gas X; .............................. (ii) gas Y. .............................. (2) (b) Why is sunlight necessary for photosynthesis? ..................................................................................................................................... ..................................................................................................................................... (1) (Total 3 marks) Page 69 Q54. The drawing shows some of the stages of reproduction in horses. (a) (i) Name this type of reproduction ....................................................................... (ii) Name the type of cell labelled A ..................................................................... (1) (1) (b) Name the type of cell division taking place at the stage labelled: (i) B ...................................................................................................................... (ii) C ...................................................................................................................... Page 70 (2) (c) How does the number of chromosomes in each cell of the embryo compare with the number of chromosomes in cell A? ..................................................................................................................................... (1) (d) When the foal grows up it will look similar to its parents but it will not be identical to either parent. (i) Explain why it will look similar to its parents. ........................................................................................................................... ........................................................................................................................... (1) (ii) Explain why it will not be identical to either of its parents. ........................................................................................................................... ........................................................................................................................... ........................................................................................................................... (2) (Total 8 marks) Q55. Describe the roles of the liver and the pancreas in the digestion of fats. ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... ............................................................................................................................................... Page 71 ............................................................................................................................................... ............................................................................................................................................... (Total 5 marks) Q56. The diagram shows a plant leaf during photosynthesis. (a) Name: (i) gas X; .............................. (ii) gas Y. .............................. (2) (b) Why is sunlight necessary for photosynthesis? ..................................................................................................................................... ..................................................................................................................................... (1) Page 72 (c) Some of the sugars produced by photosynthesis are stored as starch in the roots. Explain, as fully as you can, why it is an advantage to the plant to store carbohydrate as starch rather than as sugar. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (3) (Total 6 marks) Q57. The drawings show bolls on cotton plants. Cotton thread is made from these bolls. The size of the bolls is controlled by a single gene. This gene has two alleles. The dominant allele B is the allele for large bolls. The recessive allele b is the allele for small bolls. Use a genetic diagram to show how two cotton plants with large bolls may produce a cotton plant with small bolls. Page 73 (Total 4 marks) ## Read the passage. Designer Denim Genes USA scientists have successfully used genetic engineering to insert genes for blue pigment into cotton plants. Their aim is to get cotton plants which produce blue cotton so that denims can be manufactured without the need for dyeing. The scientists have also inserted genes that prevent cotton fibres twisting, with the aim of producing drip dry shirts made from natural fibres. Other cotton plants are being genetically engineered to produce their own insecticides. When they have perfected these new types of cotton plants, the scientists will use cloning techniques to produce large numbers of them. (i) Name the substance in cells which carries genetic information. ..................................................................................................................................... (1) (ii) Explain how molecules of this substance control characteristics such as blue colour in cotton plants. ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... ..................................................................................................................................... (3) (Total 4 marks) Page 74 Page 75 M1. (a) digested / broken down / made soluble by protease enzyme in stomach in small intestine / from stomach / from pancreas into amino acids amino acids / small molecules absorbed into blood any four for 1 mark each 4 (b) ideas that lipase / enzyme works best in alkaline / neutral conditions acid denatures or inactivates enzyme / inhibits enzyme activity bile emulsifies fat / bile produces larger surface area of fats / bile alkaline for enzyme to work on / which increase activity of enzymes any three for 1 mark each 3 [7] M2. (a) 21.5 – 22 and 27 – 27.5 for 1 mark 1 (b) ideas of limiting factor / shortage of e.g. light / carbon dioxide / water / chlorophyll each for 1 mark (allow 1 for ‘maximum / optimum rate of enzyme activity if no reference to limiting factors) (ignore denaturation) 2 (c) 21.5 – 22° C (allow first figure from answer to (i) so that no ‘double-penalty but only if this first answer is 20 or greater) maximum rate of photosynthesis / highest / fastest but related to flat part of curve most economical heating / cheapest related to heating Page 76 must relate to the temperature the candidate has given each for 1 mark 3 [6] M3. cytoplasm reject protoplasm (cell) membrane nucleus all correctly labelled each for 1 mark [3] M4. (a) Sun / sunlight / light for 1 mark 1 (b) (i) 21.5 – 22 and 27 – 27.5 for 1 mark 1 (ii) ideas of limiting factor / shortage of e.g. light / carbon dioxide / water /chlorophyll each for 1 mark (allow 1 for ‘maximum’ rate of enzyme activity if no reference to limiting factors) (ignore reference to dematuring) 2 (iii) 21.5 – 22° C (allow first figure from answer to (i) so that no ‘double-penalty’ but not below 20) Page 77 maximum rate of photosynthesis (can relate to any number on ‘flat’) most economical heating (must relate to left end of ‘flat’ each for 1 mark 3 [7] M5. (a) 10 for 1 mark 1 (b) digested / broken down / made soluble by protease / enzyme in stomach / in small intestine / from stomach / from pancreas into amino acids amino acids/smaller molecules/products of digestion absorbed into blood any four for 1 mark each 4 [5] M6. D idea that twins have come from one (fertilised) egg idea that Y sperm / Y chromosome produces boys each for 1 mark allow 1 mark if candidate selects A and states that Y sperm / Y chromosome produce boys (reject Y gene unqualified) OR allow 1 mark if candidate selects C and states that twins must have come from one (fertilised) egg [3] Page 78 M7. (a) (i) D for 1 mark 1 (ii) D Y (both) or C X (both) or B W (both) for 1 mark 1 (b) N.B. answers must relate to fossils providing evidence show types of animals / plants that no longer exist / named ref eg dinosaur show changes in types (of animals / plants) similar fossils found in rocks of similar age reference to sequence of change or example e.g. horse / limb any two for 1 mark each 2 [4] M8. (i) cytoplasm (cell) membrane nucleus all correctly labelled each for 1 mark 3 (ii) 0.5 gains 2 marks (5/100 × 10 or ½ /1 gains 1 mark if 0.5 not given) 2 [5] M9. (i) DNA Page 79 for 1 mark 1 (ii) contains the code for manufacturing the protein, as order of bases, which determine the order in which amino acids are assembled into protein for 1 mark each 3 [4] ## (a) mutation for 1 mark 1 (b) fall, idea that resistant beetles more likely to survive to breed, their offspring more likely to appear in the next generation for 1 mark each 3 (c) inbreeding between resistant brothers and sister, will produce some individuals with 2 copies of the resistance allele, if 2 of these individuals breed all their offspring will be resistant for 1 mark each 3 [7] M11. (i) the higher the rate of oxygen consumption, the shorter the time taken to complete for 1 mark 1 Page 80 (ii) the faster oxygen is taken into the blood, the faster energy can be released in the muscles, and the faster the athlete can run for 1 mark each 3 [4] M12. genotype of parent A Nn gametes N n n n young genotypes and phenotypes all correct for 1 mark each [3] ## (a) oxygen, carbon dioxide or water (vapour) for 1 mark each 2 (b) idea of more air per breath/deeper breaths for 1 mark 1 (c) (i) respiration for 1 mark 1 (ii) carbon dioxide, water for 1 mark each 2 Page 81 (iii) more energy required, for increased muscular activity for 1 mark each 2 [8] M14. (a) (i) 280 million years for 1 mark 1 (ii) e.g. the climate changed for 1 mark 1 (iii) e.g. the desert climate in the Permian was too dry for them for 1 mark 1 (b) replaced by other materials/hard parts did not decay/ exclusion of oxygen prevents decay for 1 mark 1 [4] M15. (a) (i) e.g. B and b for 1 mark 1 (ii) e.g. bb for 1 mark 1 Page 82 (b) no black genes in flock all double recessive for 1 mark each 2 [4] M16. (a) (i) June for 1 mark 1 (ii) April max. light photosynthesis makes sugars/substances needed for growth for 1 mark each 3 (b) 2 of: temperature carbon dioxide availability water chlorophyll any 2 for 1 mark each 2 [6] M17. (a) (i) bones [and feathers] 1 for 1 mark (ii) hard parts do not decay for 1 mark each 2 (iii) has feathers for 1 mark Page 83 1 (b) (i) all of kind have died out for 1 mark 1 (ii) e.g. change of habitat for 1 mark 1 (iii) named extinct organism, e.g. Dinosaur 1 for 1 mark [7] M18. (a) Stan BB Sharon bb all offspring Bb 3 (b) Tom Bb black offspring Bb white offspring bb 3 [6] M19. (a) carbon dioxide oxygen 2 (b) (i) e.g. rubber plant/fern 1 Page 84 (ii) because can tolerate low light levels 1 (iii) yellow parts of leaf do not contain chlorophyll therefore more light needed for photosynthesis 2 (iv) no leaves/only have stem only have small area which can photosynthesise 2 [8] M20. Cogently argued based on biological principles, for and against experimenting with human fertilisation techniques max 4 pros e.g. help for women who cannot conceive naturally possibility of eliminating disease causing genes possibility of inserting useful animal genes max 4 cons e.g. ethical objections religious objections social issues any 6 for 1 mark each 6 cogently argued case gains up to 2 marks 2 [8] M21. (a) (i) reduced sharply for 1 mark 1 (ii) converted to glucose which is respired to produce energy (allow answers in terms of glucagon) Page 85 gains 3 marks 3 (b) (i) athlete A’s was most effective since resulted in highest muscle glycogen level on day of race for energy release during race for 1 mark each 3 (ii) e.g. excess carbohydrate stored as glycogen rather than fat in short term particularly if glycogen stores depleted for 1 mark each 2 [9] M22. (a) (i) e.g. mussels/caddis loach for 1 mark 1 (ii) 3 of: carbon dioxide water chlorophyll/chloroplasts light any 3 for 1 mark each 3 (b) 6 of e.g. some plant/animal material not digested by consumers passes out with faeces respiration releases energy used in movement lost as heat some ‘lower’ organisms die energy transferred to decomposers/detritivores thence to environment any 6 for 1 mark each 6 [10] Page 86 M23. follow diet A because it gives the highest proportion of stored sugar in the muscles for 1 mark each [2] M24. (a) mesophyll / / / / (all correct) sperm / / x / (all correct) for 1 mark each 2 (b) (i) absorbs light/to produce food (allow reference to gaseous exchange) for 1 mark 1 (ii) e.g. has chlorophyll/chloroplasts has elongated shape to absorb light for 1 mark each 2 [5] M25. (a) mesophyll / / / / (all correct) sperm / / x / (all correct) for 1 mark each 2 Page 87 (b) (i) absorbs light/to produce food/photosynthesis (allow references to gaseous exchange) for 1 mark 1 (ii) has chlorophyll/chloroplasts to absorb light/produce food for 1 mark each (if linked to gas exchange allow – moist surface/ dissolve gases) 2 [5] M26. (a) e.g.: competition for light because potamogeton plants taller competition for nutrients taller plants may have longer roots each for 1 mark 4 (b) descriptions of: measuring tape or similar quadrat method of estimating cover (inside quadrat) each for 1 mark 3 [7] M27. 3 of e.g. new predators new diseases new competitors environmental changes (initiated by Man) each for 1 mark [3] Page 88 M28. Does not contain chlorophyll which is needed to absorb light or energy each for 1 mark [2] M29. natural variation in amount of body hair; in cold environment, (having genes) which produce long hair is an advantage; because hair insulates; OWTTE such animals more likely to survive; and pass these genes onto succeeding generations each for 1 mark [5] M30. (a) on chromosomes/DNA within the nucleus each for 1 mark 2 (b) parental genotypes correct i.e. Aa Aa; gamete genotypes correct i.e. A or a A or a/correct lines; F1 genotypes correct i.e. AA Aa Aa aa; aa recognised as child with cystic fibrosis each for 1 mark 4 (d) (i) molecule has two long strands/double helix; idea of held together by (weak) bonds; each strand has 4 different types of base; which pair with specific bases in opposite strand; Page 89 ) ) when strands separate; each strand acts as a ‘complementary’ template; makes 2 identical strands each for 1 mark ) OWTTE ) ) 6 (ii) order of bases acts as a code; which controls the order; in which amino acids are assembled into protein; read in triplet each for 1 mark 3 [15] M31. (a) oxygen; ) carbon dioxide; ) allow symbols water ) each for 1 mark 3 (b) graph with reasonable vertical scales; accurate plotting of all points (ignore lines) and labelling lines histogram – must be coded gains 3 marks 3 (c) 6 of: during exercise the level of CO2 (in the blood) rises; increased breathing to remove excess CO2; increased oxygen supply to muscles; or increased breathing takes in more O2 or increased heart rate takes more O2 to muscles; increased supply of sugar to muscles; increased respiration rate; enable faster rate of energy release; reference to lactic acid (allow even though not on syllabus)/O2 debt; to avoid cramp; anaerobic reference; reference to removal of ‘heat’; 6 Page 90 (d) high carbon dioxide concentration; brain/central nervous system; heart muscles (both) 3 [15] M32. (i) with exercise rate rises; accept between 1 – 2 minutes rate rises 1 (when exercise stops) rate falls slowly; accept gentle fall or steady fall for answers which just describe a rise then a fall allow one mark only as an alternative to the first two points 1 rate does not return to normal or to starting or to resting rate accept rate returns to normal after five minutes or three minutes of rest or after recording ended 1 (ii) 86 (per minute); 1 (iii) plotting points; deduct one mark for each error to max of two if 68 wrongly plotted count as one error (ignore the quality of the line) Page 91 2 [6] M33. (a) (i) gametes correct allow by implication from line diagram only need on X from female 1 offspring genotype correctly derived on suitable diagram or 1 (ii) 1:1 or 50% or ½ or 0.5 or 1 in 2 or 1 out of 2 or 50 : 50 do not accept 50/50 accept ‘equal’ (probability) Page 92 1 (b) Y chromosome needed for male child 1 only male has the Y or wives had only X (chromosomes) or sex determined by the sperm 1 [5] M34. (a) (i) award 1 mark for any of the mitochondria correctly labelled if a number are labelled and one is incorrect award 0 marks 1 (ii) respiration or the release or transfer of energy or it contains the enzymes for respiration do not accept energy produced 1 (b) (i) nucleus (named and correctly labelled) Page 93 arrow or line must touch or go inside the nuclear membrane 1 (ii) DNA or genes or nucleic acids accept protein or histones or nucleotides or ATGC 1 (c) enzymes or nucleus do not accept factors that affect the rate rather than control it eg pH or temperature 1 [5] M35. (a) reactants: CO2 + H2O 1 products: C6H12O6 + O2 1 balance: 6CO2 + 6H2O → C6H12O6 + 6O2 1 (b) 1 mark each for any of the following ideas: lower CO2 concentration Page 94 lower light intensity decrease water availability alter light wavelength or colour accept more green light 2 (c) (i) scales correctly constructed i.e. equal intervals along each axis 1 points plotted correctly 1 appropriate line correctly drawn accept dot to dot or line of best fit cancel if line extends through zero or beyond 50°C 1 (ii) 18 – 19 (bubbles per minute) 1 (iii) heat denatures enzymes or destroys membranes or ruptures cells or destroys cells do not accept kills enzymes 1 [10] ## (a) (i) if two nuclei drawn then maximum two marks 1 6 chromosomes 1 same 3 homologous pairs 1 nuclear membrane drawn 1 Page 95 (ii) 3 chromosomes 1 1 from each homologous pair 1 (b) (i) parent line must be separate heterozygous parents Tt × Tt maximum of 2 marks if parental genotype is wrong gametes correct T t T t 1 genotypes TT Tt Tt tt 1 (ii) correct analysis of chance i.e. 1 in 4 or 25% 1 (iii) 50% or 1 in 2 1 [10] M37. (a) Page 96 5 (b) (i) sugar or carbohydrate 1 (ii) it can be stored or it is insoluble accept it has no osmotic effect 1 (iii) any one from: respires it or releases or transfers energy turns it or stores it as fructose or sucrose or lipid or protein or cellulose 1 (c) (i) photosynthesis 1 (ii) any one from: flat surface stomata thin chloroplasts veins large surface area air spaces do not accept chlorophyll 1 [10] Page 97 M38. (a) (i) light or solar do not credit sun’s energy do not credit radiant 1 (ii) chlorophyll 1 (iii) chloroplast 1 (iv) CO2 + H2O reactants identified (accept words) 1 C6H12O6 + O2 products identified (accept words) 1 6CO2 + 6H2O → C6H12O6 + 6O2 balanced equation 1 (b) any two from: increased CO2 concentration increased water supply increased temperature (up to a point) increased light intensity do not accept heat or warmth altered light quality by less green or increasing other colours 2 (c) any four points • palisade (mesophyll) Page 98 • lots of chloroplasts or chlorophyll or main site for photosynthesis or absorb maximum amount of light • guard cells • CO2 in or O2 out or water vapour out • controls size of stoma or pores in leaf allow stomata 4 [12] M39. (a) award one mark for each key idea energy released or energy transferred or respiration allow provides or gives do not allow produces or makes 3 near to the site of movement or energy available quickly or more energy accept allows more mitochondria to fit in (mitochondria) packed (around filament) or efficient arrangement or spiral arrangement (b) contains chromosomes or genes or DNA not genetic material 1 (which) contribute half (the genes) to the fetus or offspring 23 chromosomes or half the genes Page 99 or reference to X,Y chromosome determining sex (if the notion of halfness is there) nucleus contains half genes for the offspring = 2 marks 1 [5] M40. (a) liver 1 mouth or salivary glands or duodenum or small intestine or pancreas 1 pancreas accept duodenum or ileum or small intestine do not accept stomach 1 stomach or duodenum or ileum or small intestine or pancreas 1 (b) teeth breakdown food accept chewing 1 amylase or saliva (breaks down starch) 1 (c) produces bile (salts) 1 emulsifies (fat) or produces droplets or disperses fat) 1 [8] Page 100 M41. clearly labelled ‘y’ (a) 1 mark the offspring in two horizontal rows 1 mark for each fully correct row allow transferred error if parent 2 is incorrect XX XX 1 XY XY accept YX 1 (b) parent 1 accept XX 1 (c) 50:50 or equal or even or 1:1 or 50% accept 1/2 or 2/4 1 [5] M42. (a) carbon water oxygen light chlorophyll Page 101 starch 1 mark each 6 (b) leaf (or named part of leaf) or chloroplasts accept anywhere green do not credit chlorophyll unless qualified 1 (c) water through the roots or root hairs or by osmosis do not credit where the candidate is unclear about which is which 1 CO2 through the leaf or stomata or by diffusion 1 (d) any one point: increased CO2 concentration increased water supply increased temperature (up to a point) increased light (intensity) accept altered light quality by less green or increasing other colours accept increased duration of exposure to light do not credit sun or sunshine accept CO2 from respiration 1 [10] Page 102 M43. one mark for each of the following comparisons to a maximum of 6 candidates must make a clear comparison meiosis mitosis sexual asexual gametes growth ovary or testes or gonads all other cells half number of chromsomes same number of chromosomes haploid or 23 chromosomes diploid or 46 chromosomes reassortment or variation possible or not identical no reassortment or no variation or identical 4 cells produced 2 cells produced 2 divisions 1 division [6] M44. (i) (sweet) peas 1 (ii) homozygous parents crossed [1] heterozygous (F1) offspring crossed [1] recognition of yellow dominant over green [1] recognition that results support 3:1 or 0.75 to 0.25 ratio up to 4 marks awarded for an understanding of the monohybrid cross and the expected outcome 4 [5] Page 103 M45. (a) (i) plotting values for pulse rates; 2 marks- minus 1 mark for each error to a maximum of 2 Accept values if plotted on blood volume bar chart Non-horizontal tops to bars producing variable values = 1 error If drawn as a line graph =1 mark maximum 2 (ii) Either volume of blood went up then fell; Accept went to a maximum then fell Page 104 pulse rate increased (steadily); Accept went up steadily or kept going up 2 Or at first or with low activity or with moderate activity both pulse and volume increased; Accept activity up to wall- papering with more activity pulse continued to increase but volume fell; (b) Any two of with increased activity greater muscle use or greater respiration; need more glucose or oxygen; Accept more sugar heart beat faster; Do not accept more air Accept more blood needed or blood flows faster If ‘more’ or equivalent stated once it can be accepted elsewhere by implication 2 [6] M46. (a) water [1] oxygen [1] (sun) light or solar [1] do not accept sun’s chlorophyll [1] do not accept chloroplasts 4 (b) any two from: Page 105 stored as fructose stored as sucrose stored as starch stored as oil or lipid moved or transported away in the phloem do not accept “stored” by itself respired or burnt up for energy or fuel changed to protein changed to cellulose changed to fructose changed to starch changed to oil or lipid do not accept “food for plant” do not accept “used up” by itself 2 (c) (i) roots or root hair (cells) 1 (ii) the mineral salts are (dissolved) in water [1] water transports salts throughout the plant or water enables osmosis or diffusion to take place [1] 2 (d) (i) plants grow better with some nutrients than none or plants grow better with nitrates than without comparison is needed accept “faster” as equivalent to “better” accept don’t grow well with only water 1 (ii) 0.14(g) units not needed 1 (iii) making protein or amino acids do not accept help them grow accept named protein or DNA or chlorophyll 1 Page 106 any two from: (iv) type or variety or starting weight or 2 (iii) size of seedlings keep the environment the same only if light or temperature or day length not already credited light temperature not heat time of growth do not accept the same equipment do not accept help them grow 1 day length amount of culture solution or/size of accept named protein, DNA chlorophyll boiling tube number of seedlings per tube pH CO2 humidity [15] M47. (a) (i) gametes i.e. B b and B b 1 correct combination of genotypes i.e. BB, Bb, Bb, bb 1 correct analysis of phenotypes i.e. 3 black fur 1 with brown fur 1 (ii) award one mark for the recognition that it is down to chance (which two gametes fuse) and not simply ‘because it’s a prediction’ do not accept mutation Page 107 1 (b) (i) B is dominant/ an allele is dominant if it is expressed in the heterozygous phenotype candidates are likely to use a variety of ways of expressing their ideas 1 b is recessive/ a recessive allele is not expressed in the presence of its contrasting allele do not accept powerful do not accept stronger 1 (ii) alleles are different forms of a gene controlling a characteristic and occupying the same site on homologous chromosomes (e.g. B or b) 1 genes are the units of DNA/sites on chromosomes carrying the information that determines characteristics (e.g. bB) 1 (c) homozygous: BB / bb / possessing a pair of identical alleles for a character/true breeding give credit to an explanation using a diagram 1 heterozygous: Bb / carrying a pair of contrasting/different alleles for a characteristic do not accept references to xx, xy do not accept gene by itself 1 [10] M48. (a) (i) mitosis Page 108 for 1 mark 1 (ii) 1 fertilised egg cell has 1 albino gene from father splits to produce identical cells / produced by mitosis each for 1 mark 3 (b) (i) less protection from UV light / UV radiation for 1 mark 1 (ii) ideas of uncontrolled multiplication of mutated cells reject fast / rapid cell division cells invade of other parts / cells transported in blood each for 1 mark 2 [7] M49. (i) increase in CO2 concentration leads to increase in volume of air inhaled increase of % carbon dioxide has little effect over most of range / large increase when % carbon dioxide > 5.6 % each for 1 mark 2 (ii) idea that depth of breathing changes at low % carbon dioxide, in crease in % CO2 results in volume of each breath increasing without increase / little increase in number of breaths each for 1 mark 2 [4] M50. (a) chromosomes genes (reject alleles) Page 109 alleles for 1 mark each 3 (b) (i) sexual / sex for one mark 1 (ii) egg / gamete / sex cell / ovum for one mark (reject ovule) 1 (c) (i) information / genes / DNA passed from parents (reject chromosomes) for one mark 1 (ii) genes / genetic information / chromosomes from two parents alleles may be different environmental effect / named may have been mutation any two for 1 mark each 2 [8] M51. (a) (cell) wall (cell) membrane cytoplasm vacuole for 1 mark each 4 (b) (i) A (ii) B for 1 mark each 2 Page 110 (c) diffusion (reject osmosis) for 1 mark 1 [7] M52. (a) woman XX man XY for 1 mark each 2 (b) 50% / 1 in 2 / evens / 0.5 / 50:50 for 1 mark mark scheme for genetic diagram gametes all correct genotypes of offspring all correct in relation to gametes for 1 mark each 1 mark scheme for written explanation half sperm have X chromosome, half have Y and all eggs have X chromosome 50% / 1 in 2 / evens / 0.5 chance of egg being fertilised by X or Y sperm for 1 mark each 2 [5] Page 111 M53. (a) (i) carbon dioxide / CO2 (ii) oxygen / O2 / O for 1 mark each (reject CO) (reject water vapour) 2 (b) (provides) energy for 1 mark 1 [3] M54. (a) (i) sexual / sex (ii) egg / gamete / sex cell / ovum for 1 mark each (reject ovule) 2 (b) (i) meiosis / reduction (ii) mitosis / somatic for 1 mark each 2 (c) twice as many (reject answers based on 23 / 46 chromosomes) for one mark 1 (d) (i) information / genes / DNA passed from parents (chromosomes neutral) for one mark 1 (ii) genes / genetic information / chromosomes from two parents alleles may be different environmental effect / named may have been mutation any two for 1 mark each Page 112 2 [8] M55. pancreas produces lipase which breaks down / digests fats into fatty acids and glycerol liver produces bile / hydrogen carbonate which neutralises acids / makes alkaline provides optimum / best / most effective pH for lipase / enzyme action bile emulsifies fats / description increasing the surface area for lipase / enzyme to act on any five for 1 mark each (digestion is in stomach / liver / pancreas – penalise only once) [5] M56. (a) (i) carbon dioxide / CO2 (ii) oxygen / O2 / O for 1 mark each (reject CO) (water vapour neutral) 2 (b) (provides) energy for one mark 1 (c) starch insoluble therefore water not taken in by osmosis or sugar is soluble / has small molecules may diffuse out therefore lost (ignore ref. to cells bursting) or Page 113 starch has large molecules cannot diffuse therefore retained for 1 mark each 3 [6] M57. parental genotypes both correct – both Bb gamete genotypes all correct B and b B and b genotype of bb offspring correctly related to gametes bb offspring identified as small bolls for 1 mark each [4] M58. (i) DNA (accept RNA) for one mark 1 (ii) DNA carries coded information which controls the order of amino acids in proteins for 1 mark each 3 [4] Page 114