Liang Tong
Department of Biological Sciences
1212 Amsterdam Avenue
Columbia University
New York, NY 10027
Phone: (212) 854-5203, FAX: (212) 865-8246
e-mail: ltong@columbia.edu
web: http://como.bio.columbia.edu/tong
EXPERIENCE:
September, 1997 — present
Department of Biological Sciences, Columbia University, New York, New York
Professor (July 04–present), Associate Professor (Sept. 97–June 04)
• Structural and biochemical studies of enzymes involved in fatty acid metabolism (acetyl-CoA
carboxylase, carnitine acyltransferase, AMP-activated protein kinase, pyruvate carboxylase, malic
enzyme, acyl-CoA synthetase).
• Structural and biochemical studies of pre-mRNA 3’-end processing.
• Structural and biological studies of signal transduction by Toll/interleukin-1 receptor (TIR) domains.
• Structural and biological studies of BEACH domains.
• Structural and biochemical studies of human cytomegalovirus (HCMV) protease.
• Development of new methods and computer programs for protein crystallography (program COMO).
• Design, setup and operation of a structural biology laboratory for protein expression, purification,
crystallization, and structure determination by X-ray crystallography.
August, 1992 — August, 1997
Boehringer Ingelheim Pharmaceuticals, Inc., Ridgefield, Connecticut
Principal Scientist (Jan. 96–Aug. 97), Senior Scientist (Aug. 92–Dec. 95)
• Structural studies of human p38 MAP kinase in complex with highly potent and highly specific
inhibitors, which revealed the structural basis for specificity of protein kinase inhibitors and a novel,
allosteric binding site (the DFG-out conformation).
• Structural studies of human cytomegalovirus (HCMV) protease free enzyme and inhibitor complex,
which revealed a new polypeptide backbone fold for serine proteases and the induced-fit behavior of
this protease.
• Crystal structures of inhibitor complexes of various proteins (HIV-1 reverse transcriptase, HIV-2
protease, human renin, human thrombin, human p56lck SH2 domain, etc).
• Development of new methods and computer programs for protein crystallography. (The Replace
program suite, locked translation function, combined molecular replacement)
• Crystal structures of small molecules (inhibitors, reaction intermediates).
• Design, setup and operation of an X-ray diffraction laboratory for the structure determination of
proteins and small molecules.
August, 1989 — July, 1992
Purdue University, West Lafayette, Indiana
Post-Doctoral Research Associate (Prof. Michael G. Rossmann)
• Crystal structure of Sindbis virus capsid protein. The structure was determined by a combination of
the multiple isomorphous replacement and the molecular replacement averaging techniques and
revealed a chymotrypsin-like serine proteinase.
• Development of new methods and computer programs for protein crystallography. (Locked rotation
function (GLRF), translation function, Patterson map interpretation, real and reciprocal space
molecular replacement averaging)
August, 1984 — July, 1989
University of California, Berkeley
Graduate Research Assistant (Prof. Sung-Hou Kim)
1
• Crystal structure determination, by the multiple isomorphous replacement method, and refinement of
the catalytic domain of the human ras proto-oncogene product p21 and its oncogenic mutants.
• Crystal structure refinement of the sweet-tasting protein monellin.
Teaching Assistant
• Freshman chemistry, graduate level introduction to X-ray crystallography and biophysical chemistry.
January, 1983 — July, 1983
Peking University, Beijing, China
Undergraduate Research Assistant (Prof. You-Qi Tang)
• The characterization and crystal structure determination of the reaction product between (NH4)2MoS4
and the cupric salts of amino acids, and its relevance to Cu-Mo antagonism.
EDUCATION:
Ph. D., Biophysical Chemistry. December, 1989
University of California, Berkeley
B. Sc., Chemistry. July, 1983
Peking University (Beijing, P.R. China)
HONORS:
Phi Beta Kappa, 1989; Sigma Xi, 1991.
The Vice President’s Golden Achievement Award, 1996. Boehringer Ingelheim Pharmaceuticals, Inc.
Ridgefield, CT.
The first Boehringer Ingelheim worldwide Research and Development Award, 1997.
EDITORIAL BOARD:
J. Biol. Chem. (July 2006-)
Protein and Peptide Letters, Editor for Eastern USA (Oct. 2006-)
Journal of Structural and Functional Genomics (Jan. 2007-)
Science in China B: Chemistry (Nov. 2008-)
Faculty of 1000 (2004-2007)
Biologics: Targets and Therapy (2007-)
Protein and Peptide Letters (Jan. 2006-Oct. 2006)
2
PUBLICATIONS (135 papers + 24 reviews/book chapters):
Peer-reviewed publications
1.
X.-L. Jin, K.-L. Tang, L. Tong & Y.-Q. Tang. (1984). The reactions of ammonium thiomolybdate
with amino-acid salts of copper(II) and the crystal structure of the product Cu2MoS4(py)4. Nature
journal (China), 7, 314-316.
2.
X.-L. Jin, K.-L. Tang, L. Tong & Y.-Q. Tang. (1986). The reaction between (NH4)2MoS4 and the
cupric salts of amino acids and the crystal structure of the reaction product Cu2MoS4(py)4.
Scientia Sinica (Chinese Edition), B11, 1136-1142.
3.
A.M. de Vos, L. Tong, M.V. Milburn, P.M. Matias, J. Jancarik, S. Noguchi, S. Nishimura, K.
Miura, E. Ohtsuka & S.-H. Kim. (1988). Three-dimensional structure of an oncogene protein:
catalytic domain of human c-H-ras p21. Science, 239, 888-893.
4.
R.M. Glaeser, L. Tong & S.-H. Kim. (1989). Three-dimensional reconstructions from incomplete
data: Interpretability of density maps at "atomic" resolution.Ultramicroscopy, 27, 307-318.
5.
L. Tong, M.V. Milburn, A.M. de Vos & S.-H. Kim. (1989). Structure of ras protein. Science,
245, 244-244.
6.
M.V. Milburn, L. Tong, A.M. de Vos, A. Brünger, Z. Yamaizumi, S. Nishimura & S.-H. Kim.
(1990). Molecular switch for signal transduction: Structural differences between active and
inactive forms of protooncogenic ras proteins. Science, 247, 939-945.
7.
A.T. Brünger, M.V. Milburn, L. Tong, A.M. de Vos, J. Jancarik, Z. Yamaizumi, S. Nishimura, E.
Ohtsuka & S.-H. Kim. (1990). Crystal structure of an active form of RAS protein, a complex of a
GTP analog and the HRAS p21 catalytic domain. Proc. Natl. Acad. Sci. U.S.A. 87, 4849-4853.
8.
L. Tong & M.G. Rossmann. (1990). The locked rotation function. Acta Cryst. A46, 783-792.
9.
L. Tong, A.M. de Vos, M.V. Milburn & S.-H. Kim. (1991). Crystal structures at 2.2Å resolution
of the catalytic domains of normal ras protein and an oncogenic mutant complexed with GDP. J.
Mol. Biol. 217, 503-516.
10. H.-K. Choi,* L. Tong,* W. Minor, P. Dumas, U. Boege, M.G. Rossmann & G. Wengler. (1991).
Structure of Sindbis virus core protein reveals a chymotrypsin-like serine proteinase and the
organization of the virion. Nature, 354, 37-43. (*–equal first authors; News and Views feature
on p. 22-23)
11. M.G. Rossmann, R. McKenna, L. Tong, D. Xia, J.-B. Dai, H. Wu, H.-K. Choi & R.E. Lynch.
(1992). Molecular replacement real space averaging. J. Appl. Cryst. 25, 166-180.
12. G.G. Privé, M.V. Milburn, L. Tong, A.M. de Vos, Z. Yamaizumi, S. Nishimura & S.-H. Kim.
(1992). X-ray crystal structures of transforming p21 ras mutants suggest a transition-state
stabilization mechanism for GTP hydrolysis. Proc. Natl. Acad. Sci. U.S.A. 89, 3649-3653.
13. L. Tong, H.-K. Choi, W. Minor & M.G. Rossmann. (1992). Structure determination of Sindbis
virus core protein using isomorphous replacement and molecular replacement averaging between
two crystal forms. Acta Cryst. A48, 430-442.
14. L. Tong, G. Wengler & M.G. Rossmann. (1993). Refined structure of Sindbis virus core protein
and comparison with other chymotrypsin-like serine proteinase structures. J. Mol. Biol. 230, 228247.
15. L. Tong & M.G. Rossmann. (1993). Patterson-map interpretation with noncrystallographic
symmetry. J. Appl. Cryst. 26, 15-21.
16. L. Tong, M. Cardozo, P.-J. Jones & J. Adams. (1993). Preliminary structural analysis of the
mutations selected by non-nucleoside inhibitors of HIV-1 reverse transcriptase. Bioorg. & Med.
Chem. Lett. 3, 721-726.
17. L. Tong, S. Pav, C. Pargellis, F. Dô, D. Lamarre & P.C. Anderson. (1993). Crystal structure of
human immunodeficiency virus (HIV) type 2 protease in complex with a reduced amide inhibitor
and comparison with HIV-1 protease structures. Proc. Natl. Acad. Sci. U.S.A. 90, 8387-8391.
(Corresponding author)
3
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
33.
34.
K. Pal, M.L. Behnke & L. Tong. (1993). A general stereocontrolled synthesis of cis-2,3
disubstituted pyrrolidines and piperidines. Tetrahedron Lett. 34, 6205-6208.
L. Tong. (1993). REPLACE, a suite of computer programs for molecular-replacement
calculations. J. Appl. Cryst. 26, 748-751.
J.R. Somoza, F. Jiang, L. Tong, C.-H. Kang, J.M. Cho & S.-H. Kim. (1993). Two crystal
structures of a potently sweet protein. Natural monellin at 2.75Å resolution and single-chain
monellin at 1.7Å resolution. J. Mol. Biol. 234, 390-404.
S. Pav, K. Lubbe, F. Dô, D. Lamarre, C. Pargellis & L. Tong. (1994). Microtube batch protein
crystallization: Applications to human immunodeficiency virus type 2 (HIV-2) protease and
human renin. Proteins: Structure, Function, and Genetics, 20, 98-102.
R.J. Snow, W.W. Bachovchin, R.W. Barton, S.J. Campbell, S.J. Coutts, D.M. Freeman, W.G.
Gutheil, T.A. Kelly, C.A. Kennedy, D.A. Krolikowski, S.F. Leonard, C.A. Pargellis, L. Tong & J.
Adams. (1994). Studies on proline boronic acid dipeptide inhibitors of dipeptidyl peptidase IV:
Identification of a cyclic species containing a B-N bond. J. Amer. Chem. Soc. 116, 10860-10869.
L. Tong, S. Pav, S. Mui, D. Lamarre, C. Yoakim, P. Beaulieu & P.C. Anderson. (1995). Crystal
structures of HIV-2 protease in complex with inhibitors containing the hydroxyethylamine
dipeptide isostere. Structure, 3, 33-40. (Corresponding author)
L. Tong, S. Pav, D. Lamarre, L. Pilote, S. LaPlante, P.C. Anderson & G. Jung. (1995). High
resolution crystal structures of recombinant human renin in complex with
polyhydroxymonoamide inhibitors. J. Mol. Biol. 250, 211-222. (Corresponding author)
L. Tong & M.G. Rossmann. (1995). Reciprocal space molecular replacement averaging. Acta
Cryst. D51, 347-353.
M.A. Cornea-Hasegan, Z. Zhang, R.E. Lynch, D.C. Marinescu, A. Hadfield, J.K. Muckelbauer,
S. Munshi, L. Tong & M.G. Rossmann. (1995). Phase refinement and extension by means of
non-crystallographic symmetry averaging using parallel computers. Acta Cryst. D51, 749-759.
J.R. Proudfoot, K.D. Hargrave, S. Kapadia, U.R. Patel, K.G. Grozinger, D.W. McNeil, E. Cullen,
M. Cardozo, L. Tong, T.A. Kelly, J. Rose, E. David, S.C. Mauldin, V.U. Fuchs, J. Vitous, M.
Hoermann, J.M. Klunder, P. Raghavan, J.W. Skiles, P. Mui, D.D. Richman, J.L. Sullivan, C.-K.
Shih, P.M. Grob & J. Adams. (1995). Novel non-nucleoside inhibitors of human
immunodeficiency virus type 1 (HIV-1) reverse transcriptase. 4. 2-Substituted
dipyridodiazepinones as potent inhibitors of both wild-type and cysteine-181 HIV-1 reverse
transcriptase enzymes. J. Med. Chem. 38, 4830-4838.
L. Tong, S. Pav, D. Lamarre, B. Simoneau, P. Lavallée & G. Jung. (1995). Crystallographic
studies on the binding modes of P2-P3 butanediamide renin inhibitors. J. Biol. Chem. 270,
29520-29524. (Corresponding author)
J.K. Muckelbauer, M. Kremer, I. Minor, L. Tong, A. Zlotnick, J.E. Johnson & M.G. Rossmann.
(1995). The structure determination of Coxsackievirus B3 to 3.5Å resolution. Acta Cryst. D51,
871-887.
S.J. Harrop, J.R. Helliwell, T.C.M. Wan, A.J. Kalb (Gilboa), L. Tong & J. Yariv. (1996).
Structure solution of a cubic crystal of Concanavalin A complexed with methyl -Dglucopyranoside. Acta Cryst. D52, 143-155.
L. Tong, T.C. Warren, J. King, R. Betageri, J. Rose & S. Jakes. (1996). Crystal structures of the
human p56lck SH2 domain in complex with two short phosphotyrosyl peptides at 1.0Å and 1.8Å
resolution. J. Mol. Biol. 256, 601-610. (Corresponding author)
L. Tong. (1996). The locked translation function and other applications of a Patterson correlation
function. Acta Cryst. A52, 476-479.
G.P. Roth, S.F. Leonard & L. Tong. (1996). Complementary selectivity in the alkylation of chiral
bicyclic lactam enolates. J. Org. Chem. 61, 5710-5711.
L. Tong, C. Qian, M.-J. Massariol, P.R. Bonneau, M.G. Cordingley & L. Lagacé. (1996). A new
serine-protease fold revealed by the crystal structure of human cytomegalovirus protease. Nature,
383, 272-275. (Corresponding author)
4
35.
36.
37.
38.
39.
40.
41.
42.
43.
44.
45.
46.
47.
48.
49.
50.
51.
L. Tong. (1996). Combined molecular replacement. Acta Cryst. A52, 782-784.
C. Momany, L.C. Kovari, A.J. Prongay, W. Keller, R.K. Gitti, B.M. Lee, A.E. Gorbalenya, L.
Tong, J. McClure, L.S. Ehrlich, M.F. Summers, C. Carter & M. G. Rossmann. (1996). Crystal
structure of dimeric HIV-1 capsid protein. Nature Struct. Biol. 3, 763-770.
S.R. LaPlante, L. Tong, N. Aubry, S. Pav, G. Jung & P.C. Anderson. (1996). Several
polyhydroxymonoamide renin inhibitors assume similar conformations in the unbound and reninbound states. Int. J. Pept. Protein Res. 48, 401-410.
S. Pav, D.M. White, S. Rogers, K.M. Crane, C.L. Cywin, W. Davidson, J. Hopkins, M.L. Brown,
C.A. Pargellis & L. Tong. (1997). Crystallization and preliminary crystallographic analysis of
recombinant human p38 MAP kinase. Protein Science, 6, 242-245.
G.P. Roth, M. Emmanuel & L. Tong. (1997). Resolution and assignment of the absolute
stereochemistry of a trans-2,3-diarylpyrrolidine LTB4 inhibitor intermediate.
Tetrahedron:Asymmetry, 8, 185-186.
L. Tong, S. Pav, D.M. White, S. Rogers, K.M. Crane, C.L. Cywin, M.L. Brown & C.A. Pargellis.
(1997). A highly specific inhibitor of human p38 MAP kinase binds in the ATP pocket. Nature
Struct. Biol. 4, 311-316. (Corresponding author)
P.L. Beaulieu, D. Wernic, A. Abraham, P.C. Anderson, T. Bogri, Y. Bousquet, G. Croteau, I.
Guse, D. Lamarre, F. Liard, W. Paris, D. Thibeault, S. Pav & L. Tong. (1997). Potent HIV
protease inhibitors containing a novel (hydroxyethyl)amide isostere. J. Med. Chem. 40, 21642176.
L. Tong, C. Qian, W. Davidson, M.-J. Massariol, P.R. Bonneau, M.G. Cordingley & L. Lagacé.
(1997). Experiences from the structure determination of human cytomegalovirus protease. Acta
Cryst. D53, 682-690. (Corresponding author)
L. Tong, T.C. Warren, S. Lukas, J. Schembri-King, R. Betageri, J.R. Proudfoot & S. Jakes.
(1998). Carboxymethyl-phenylalanine as a replacement for phosphotyrosine in SH2 domain
binding. J. Biol. Chem. 273, 20238-20242. (Corresponding author)
L. Tong, C. Qian, M.-J. Massariol, R. Deziel, C. Yoakim & L. Lagacé. (1998). Conserved mode
of peptidomimetic inhibition and substrate recognition of human cytomegalovirus protease.
Nature Struct. Biol. 5, 819-826. (Corresponding author)
Y.C. Park, V. Burkitt, A.R. Villa, L. Tong & H. Wu. (1999). Structural basis for self-association
and receptor recognition of human TRAF-2. Nature, 398, 533-538.
S.R. LaPlante, P.R. Bonneau, N. Aubry, D.R. Cameron, R. Deziel, C. Grand-Maitre, C. Plouffe,
L. Tong & S. H. Kawai (1999). Characterization of the human cytomegalovirus protease as an
induced-fit protease and the implications to the design of mechanism-based peptidyl inhibitors. J.
Amer. Chem. Soc. 121, 2974-2986.
P.L. Beaulieu, D.R. Cameron, J.-M. Ferland, J. Gauthier, E. Ghiro, J. Gillard, V. Gorys, M.
Poirier, J. Rancourt, D. Wernic, M. Llinas-Brunet, R. Betageri, M. Cardozo, E.R. Hickey, R.
Ingraham, S. Jakes, A. Kabcenell, T. Kirrane, S. Lukas, U. Patel, J. Proudfoot, R. Sharma, L.
Tong & N. Moss. (1999). Ligands for the tyrosine kinase p56lck SH2 domain: Discovery of
potent dipeptide derivatives with monocharged, nonhydrolyzable phosphate replacements. J.
Med. Chem. 42, 1757-1766.
G. Bhargava, S. Mui, S. Pav, H. Wu, G. Loeber & L. Tong. (1999). Preliminary crystallographic
studies of human mitochondrial NAD(P)+-dependent malic enzyme. J. Struct. Biol. 127, 72-75.
Y. Xu, G. Bhargava, S. Mui, S. Pav, G. Loeber, H. Wu & L. Tong. (1999). Crystal structure of
human mitochondrial NAD(P)+-dependent malic enzyme: a new class of oxidative
decarboxylases. Structure, 7, 877-889.
C. Qian, L. Lagacé, M.-J. Massariol, C. Chabot, C. Yoakim, R. Deziel & L. Tong. (2000). A
rational approach towards successful crystallization and crystal treatment of human
cytomegalovirus protease and its inhibitor complex. Acta Cryst. D56, 175-180.
Z. Yang, D.L. Floyd, G. Loeber & L. Tong. (2000). Structure of closed form of malic enzyme and
implications for catalytic mechanism. Nature Struct. Biol. 7, 251-257.
5
52.
53.
54.
55.
56.
57.
58.
59.
60.
61.
62.
63.
64.
65.
66.
67.
68.
W.-Y. Chou, H.-P. Chang, C.-H. Huang, C.-C. Kuo, L. Tong & G.-G. Chang. (2000).
Characterization of the functional role of Asp141, Asp194, and Asp464 residues in the Mn2+-Lmalate binding of pigeon liver malic enzyme. Protein Sci. 9, 242-251.
P. L. Beaulieu, P. C. Anderson, D. R. Cameron, G. Croteau, V. Gorys, C. Grand-Maitre, D.
Lamarre, F. Liard, W. Paris, L. Plamondon, F. Soucy, D. Thibeault, D. Wernic, C. Yoakim, S.
Pav & L. Tong. (2000). 2’,6’-dimethylphenoxyacetyl: a new achiral high affinity P3-P2 ligand for
hydroxyethylamine-based HIV protease inhibitors. J. Med. Chem. 43, 1094-1108.
Z. Yang, R. Batra, D.L. Floyd, H.-C. Hung, G.-G. Chang & L. Tong. (2000). Potent and
competitive inhibition of malic enzymes by lanthanide ions. Biochem. Biophys. Res. Commun.
274, 440-444.
Y. Xu, X. Tao, B. Shen, T. Horng, R. Medzhitov, J.L. Manley & L. Tong. (2000). Structural basis
for signal transduction by the Toll/Interleukin-1 receptor (TIR) domains. Nature, 408, 111-115.
H.-C. Hung, G.-G. Chang, Z. Yang & L. Tong. (2000). Slow binding of metal ions to pigeon liver
malic enzyme: a general case. Biochem. 39, 14095-14102.
D. Shi, H. Morizono, X. Yu, L. Tong, N. M. Allewell & M. Tuchman. (2001). Crystallization and
preliminary X-ray crystallographic studies of wild-type human ornithine transcarbamylase and
two naturally occurring mutants at position 277. Acta Cryst. D57, 719-721.
D. Shi, H. Morizono, X. Yu, L. Tong, N. M. Allewell & M. Tuchman. (2001). Human ornithine
transcarbamylase: Crystallographic insights into substrate recognition and conformational
changes. Biochem. J. 354, 501-509.
S. Rozovsky, G. Jogl, L. Tong & A.E. McDermott. (2001). Solution-state NMR investigations of
triosephosphate isomerase active site loop motion: Ligand release in relation to active site loop
dynamics. J. Mol. Biol. 310, 271-280.
R. Khayat, R. Batra, M.-J. Massariol, L. Lagace & L. Tong. (2001). Investigating the role of
histidine157 in the catalytic activity of human cytomegalovirus protease. Biochem. 40, 63446351.
K. Last-Barney, W. Davidson, M. Cardozo, L. L. Frye, C. A. Grygon, J. L. Hopkins, D. D.
Jeanfavre, S. Pav, C. Qian, J. M. Stevenson, L. Tong, R. Zindell & T. A. Kelly. (2001). Binding
site identification of hydantoin-based antagonists of LFA-1 using multidisciplinary technologies:
Evidence for the allosteric inhibition of a protein-protein interaction. J. Amer. Chem. Soc. 123,
5643-5650.
G. Jogl, X. Tao, Y. Xu & L. Tong. (2001). COMO: A program for combined molecular
replacement. Acta Cryst. D57, 1127-1134.
R. Batra, R. Khayat & L. Tong. (2001). Molecular mechanism for dimerization to regulate the
catalytic activity of human cytomegalovirus protease. Nature Struct. Biol. 8, 810-817.
M.A. Rishavy, Z. Yang, L. Tong & W.W. Cleland. (2001). Determination of the mechanism of
human malic enzyme with natural and alternate dinucleotides by isotope effects. Arch. Biochem.
Biophys. 396, 43-48.
Z. Yang, H. Zhang, H.-C. Hung, C.-C. Kuo, L.-C. Tsai, H.S. Yuan, W.-Y. Chou, G.-G. Chang &
L. Tong. (2002). Structural studies of pigeon cytosolic NADP+-dependent malic enzyme. Protein
Science, 11, 332-341.
C. Pargellis,* L. Tong,* L. Churchill, P.F. Cirillo, T. Gilmore, A.G. Graham, P.M. Grob, E.R.
Hickey, N. Moss, S. Pav & J. Regan. (2002). Inhibition of p38 MAP kinase by utilizing a novel
allosteric binding site. Nature Struct. Biol. 9, 268-272. (*–co-corresponding authors)
J. Regan, S. Breitfelder, P. Cirillo, T. Gilmore, A.G. Graham, E. Hickey, B. Klaus, J. Madwed,
M. Moriak, N. Moss, C. Pargellis, S. Pav, A. Proto, A. Swanimer, L. Tong & C. Torcellini.
(2002). Pyrazole urea-based inhibitors of p38 MAP kinase: From lead compound to clinical
candidate. J. Med. Chem. 45, 2994-3008.
Z. Yang, C.W. Lanks & L. Tong. (2002). Molecular mechanism for the regulation of human
mitochondrial NAD+-dependent malic enzyme by fumarate and ATP. Structure, 10, 951-960.
6
69.
70.
71.
72.
73.
74.
75.
76.
77.
78.
79.
80.
81.
82.
83.
84.
85.
86.
R. Batra, D. Christendat, A. Edwards, C. Arrowsmith & L. Tong. (2002). Crystal structure of
MTH169, a crucial component of phosphoribosylformylglycinamidine synthetase. Proteins, 49,
285-288.
G. Jogl, Y. Shen, D. Gebauer, J. Li, K. Wiegmann, H. Kashkar, M. Kronke & L. Tong. (2002).
Crystal structure of the BEACH domain reveals an unusual fold and extensive association with a
novel PH domain. EMBO J. 21, 4785-4795.
X. Tao, Y. Xu, Y. Zheng, A. Beg & L. Tong. (2002). An extensively-associated dimer in the
structure of the C713S mutant of the TIR domain of human TLR2. Biochem. Biophys. Res.
Commun. 299, 216-221.
G. Jogl & L. Tong. (2003). Crystal structure of carnitine acetyltransferase and implications for the
catalytic mechanism and fatty acid transport. Cell, 112, 113-122.
G. Jogl, S. Rozovsky, A.E. McDermott & L. Tong. (2003). Optimal alignment for enzymatic
proton transfer: Structure of the Michaelis complex of triosephosphate isomerase at 1.2-Å
resolution. Proc. Natl. Acad. Sci. USA, 100, 50-55.
R. Khayat, R. Batra, C. Qian, T. Halmos, M. Bailey & L. Tong. (2003). Structural and
biochemical studies of inhibitor binding to human cytomegalovirus protease. Biochem. 42, 885891.
Z. Yang, A. Savchenko, A. Yakunin, R. Zhang, A. Edwards, C. Arrowsmith & L. Tong. (2003).
Aspartate dehydrogenase, a novel enzyme identified from structural and functional studies of
TM1643. J. Biol. Chem. 278, 8804-8808.
H. Zhang, Z. Yang,* Y. Shen* & L. Tong. (2003). Crystal structure of the carboxyltransferase
domain of acetyl-coenzyme A carboxylase. Science, 299, 2064-2067. (*–equal second authors)
X. Tao, R. Khayat, D. Christendat, A. Savchenko, X. Xu, S. Goldsmith-Fischman, B. Honig, A.
Edwards, C.H. Arrowsmith & L. Tong. (2003). Crystal structures of MTH1187 and its yeast
ortholog YBL001c. Proteins, 52, 478-480.
X. Tao & L. Tong. (2003). Crystal structure of DJ-1, a protein associated with early-onset
Parkinson’s disease. J. Biol. Chem. 278, 31372-31379.
X. Tao, Z. Yang & L. Tong. (2003). Crystal structures of substrate complexes of malic enzyme
and insights into the catalytic mechanism. Structure, 11, 1141-1150.
F. Forouhar, J. Shen, R. Xiao, T.B. Acton, G.T. Montelione & L. Tong. (2003). Functional
assignment based on structural analysis: Crystal structure of the yggJ protein (HI0303) of
Haemophilus influenzae reveals an RNA methyltransferase with a deep trefoil knot. Proteins. 53,
329-332.
S. Gobin, L. Thuillier, G. Jogl, A. Faye, L. Tong, M. Chi, J.-P. Bonnefont, J. Girard, C. PripBuus. (2003). Functional and structural basis of carnitine palmitoyltransferase 1A deficiency. J.
Biol. Chem. 278, 50428-50434.
R. Khayat, R. Batra, G.A. Bebernitz, M.W. Olson & L. Tong. (2004). Characterization of the
monomer-dimer equilibrium of human cytomegalovirus protease by kinetic methods. Biochem.
43, 316-322.
C.-W. Kuo, H.-C. Hung, L. Tong & G.-G. Chang. (2004). Metal-induced reversible structural
interconversion of human mitochondrial NAD(P)+-dependent malic enzyme. Proteins, 54, 404411.
G. Jogl & L. Tong. (2004). Crystal structure of yeast acetyl-CoA synthetase in complex with
AMP. Biochem. 43, 1425-1431.
F. Forouhar, I. Lee, J. Benach, K. Kulkarni, R. Xiao, T.B. Acton, G.T. Montelione & L. Tong.
(2004). A novel NAD binding protein revealed by the crystal structure of 2,3-diketo-L-gulonate
reductase (YiaK). J. Biol. Chem. 279, 13148-13155.
H. Zhang, B. Tweel & L. Tong. (2004). Molecular basis for the inhibition of the
carboxyltransferase domain of acetyl-coenzyme A carboxylase by haloxyfop and diclofop. Proc.
Natl. Acad. Sci. USA, 101, 5910-5915.
7
87.
88.
89.
90.
91.
92.
93.
94.
95.
96.
97.
98.
99.
100.
101.
102.
103.
104.
105.
W.-C. Hsu, H.-C. Hung, L. Tong & G.-G. Chang. (2004). Dual functional roles of ATP in the
human mitochondrial malic enzyme. Biochem. 43, 7382-7390.
Y.-H. Chen, M.-H. Li, Y. Zhang, L.-L. He, Y. Yamada, A. Fitzmaurice, Y. Shen, H. Zhang, L.
Tong & J. Yang. (2004). Structural basis of 1- subunit interaction of voltage-gated Ca2+
channels. Nature, 429, 675-680.
Y.-S. Hsiao, G. Jogl & L. Tong. (2004). Structural and biochemical studies of the substrate
selectivity of carnitine acetyltransferase. J. Biol. Chem. 279, 31584-31589.
J.A. Khan, E.K. Brint, L.A.J. O’Neill & L. Tong. (2004). Crystal structure of the Toll/interleukin1 receptor (TIR) domain of IL-1RAPL. J. Biol. Chem. 279, 31664-31670.
H. Zhang, B. Tweel, J. Li & L. Tong. (2004). Crystal structure of the carboxyltransferase domain
of acetyl-coenzyme A carboxylase in complex with CP-640186. Structure, 12, 1683-1691.
R. Hering,* K.M. Strauss,* X. Tao,* A. Bauer, D. Woitalla, E.-M. Mietz, S. Petrovic, P. Bauer,
W. Schaible, T. Müller, L. Schöls, C. Klein, D. Berg, P.T. Meyer, J.B. Schulz, B. Wollnik, L.
Tong, R. Krüger & O. Riess. (2004). Novel homozygous E64D mutation in DJ1 in early onset
Parkinson disease (PARK7). Hum. Mutat. 24, 321-329. (*–equal first author)
D. Gebauer,* J. Li,* G. Jogl, Y. Shen, D.G. Myszka & L. Tong. (2004). Crystal structure of the
PH-BEACH domains of human LRBA/BGL. Biochem. 43, 14873-14880. (*–equal first authors)
W. Blankenfeldt, A.P. Kuzin, T. Skarina, Y. Korniyenko, L. Tong, P. Beyer, P. Janning, L.S.
Thomashow & D.V. Mavrodi. (2004). Structure and function of the phenazine biosynthetic
protein PhzF from Pseudomonas fluorescens. Proc. Natl. Acad. Sci. USA, 101, 16431-16436.
Y. Shen, S.L. Volrath, S.C. Weatherly, T.D. Elich & L. Tong. (2004). A mechanism for the
potent inhibition of eukaryotic acetyl coenzyme A carboxylase by soraphen A, a macrocyclic
polyketide natural product. Mol. Cell, 16, 881-891.
G. Jogl, Y.-S. Hsiao & L. Tong. (2005). Crystal structure of murine carnitine octanoyltransferase
and molecular determinants of substrate selectivity. J. Biol. Chem. 280, 738-744.
F. Forouhar, Y. Yang,$ D. Kumar,$ C. Yang, Y. Fridman, S.W. Park, Y. Chiang, T.B. Acton, G.T.
Montelione, E. Pichersky,* D.F. Klessig* & L. Tong.* (2005). Structural and biochemical studies
identify SABP2 as a methylsalicylate esterase and implicate it in plant innate immunity. Proc.
Natl. Acad. Sci. USA, 102, 1773-1778. ($–equal second authors; *–co-corresponding authors)
J.A. Khan, B.M. Dunn & L. Tong. (2005). Crystal structure of human Taspase1, a crucial
protease regulating the function of MLL. Structure, 13, 1443-1452.
M.J. Rudolph, G.A. Amodeo, Y. Bai & L. Tong. (2005). Crystal structure of the protein kinase
domain of yeast AMP-activated protein kinase Snf1. Biochem. Biophys. Res. Commun. 337,
1224-1228
F. Forouhar, I.-S. Lee, J. Vujcic, S. Vujcic, J. Shen, S.M. Vorobiev, R. Xiao, T.B. Acton, G.T.
Montelione, C.W. Porter & L. Tong. (2005). Structural and functional evidence for Bacillus
subtilis PaiA as a novel N1-spermidine/spermine acetyltransferase (SSAT). J. Biol. Chem. 280,
40328-40336.
S.C. Annedi, F. Biabani, E. Poduch, B.M. Mannargudi, K. Majumder, L. Wei, R. Khayat, L.
Tong & L.P. Kotra. (2006). Engineering D-amino acid containing novel protease inhibitors using
catalytic site architecture. Bioorg. Med. Chem. 14, 214-236.
Y. Shen, C.-Y. Chou, G.-G. Chang & L. Tong. (2006). Is dimerization required for the catalytic
activity of bacterial biotin carboxylase? Mol. Cell, 22, 807-818.
J.A. Khan, X. Tao & L. Tong. (2006). Molecular basis for the inhibition of human NMPRTase, a
novel target for anti-cancer agents. Nature Struct. Mol. Biol. 13, 582-588. (News & Views 563564)
Y.-S. Hsiao, G. Jogl, V. Esser & L. Tong. (2006). Crystal structure of rat carnitine
palmitoyltransferase II (CPT-II). Biochem. Biophys. Res. Commun. 346, 974-980.
G. Soong, A. Muir, M.I. Gomez, J. Waks, B. Reddy, P. Planet, P.K. Singh, Y. Kanetko, M.C.
Wolfgang, Y.-S. Hsiao, L. Tong & A. Prince. (2006). Bacterial neuraminidase facilitates mucosal
infection by participating in biofilm production. J. Clin. Invest. 116, 2297-2305.
8
106. Y.-S. Hsiao, G. Jogl & L. Tong. (2006). Crystal structures of murine carnitine acetyltransferase in
ternary complexes with its substrates. J. Biol. Chem. 281, 28480-28487.
107. C.R. Mandel, D. Gebauer, H. Zhang & L. Tong. (2006). A serendipitous discovery that in situ
partial proteolysis is essential for the crystallization of yeast CPSF-100 (Ydh1p). Acta Cryst. F62,
1041-1045.
108. C.R. Mandel, S. Kaneko,* H. Zhang,* D. Gebauer, V. Vethantham, J.L. Manley & L. Tong.
(2006). Polyadenylation factor CPSF-73 is the pre-mRNA 3’-end-processing endonuclease.
Nature, 444, 953-956. (*–equal second authors)
109. F. Forouhar,* J.L.R. Anderson,* C.G. Mowat,* S.M. Vorobiev, A. Hussain, M. Abashidze, C.
Bruckmann, S.J. Thackray, J. Seetharaman, T. Tucker, R. Xiao, L.-C. Ma, L. Zhao, T.B. Acton,
G.T. Montelione,$ S.K. Chapman$ & L. Tong.$ (2007). Molecular insights into catalysis by
tryptophan 2,3-dioxygenase. Proc. Natl. Acad. Sci. USA, 104, 473-478. (*–equal first authors, $–
co-corresponding authors)
110. M.J. Rudolph, G.A. Amodeo, S.H. Iram, S.-P. Hong, G. Pirino, M. Carlson & L. Tong. (2007).
Crystal structure of the Bateman2 domain of yeast Snf4: dimeric association and relevance for
AMP binding. Structure, 15, 65-74.
111. S. Xiang, G. Usunow, G. Lange, M. Busch & L. Tong. (2007). Crystal structure of 1-deoxy-Dxylulose 5-phosphate synthase (DXS), a crucial enzyme for isoprenoids biosynthesis. J. Biol.
Chem. 282, 2676-2682.
112. Y. Bai, T.C. Auperin & L. Tong. (2007). The use of in situ proteolysis in the crystallization of
murine CstF-77. Acta Cryst. F63, 135-138.
113. S.M. Vorobiev, H. Neely, J. Seetharaman, L.-C. Ma, R. Xiao, T.B. Acton, G.T. Montelione & L.
Tong. (2007). Crystal structure of AGR_C_4470p from Agrobacterium tumefaciens. Protein Sci.
16, 535-538.
114. Y. Bai, T.C. Auperin, C.-Y. Chou, G.-G. Chang, J.L. Manley & L. Tong. (2007). Crystal structure
of murine CstF-77: Dimeric association and implications for polyadenylation of mRNA
precursors. Mol. Cell, 25, 863-875.
115. X. Tao & L. Tong. (2007). Crystal structure of the MAP kinase binding domain and the catalytic
domain of human MKP5. Protein Sci. 16, 880-886.
116. J. Li, X. Mao, L.Q. Dong, F. Liu & L. Tong. (2007). Crystal structures of the BAR-PH and PTB
domains of human APPL1. Structure, 15, 525-533.
117. C. Yin,* J.A. Khan,* G.V.T. Swapna, A. Ertekin, R.M. Krug,$ L. Tong$ & G.T. Montelione.$
(2007). Conserved surface features form the double-stranded RNA-binding site of non-structural
proteins 1 (NS1) from influenza A and B viruses. J. Biol. Chem. 282, 20584-20592. (*–equal
first authors, $–co-corresponding authors)
118. J.A. Khan,* S. Xiang* & L. Tong. (2007). Crystal structure of human nicotinamide riboside
kinase (NRK1). Structure, 15, 1005-1013. (*–equal first authors)
119. G.A. Amodeo,* M.J. Rudolph* & L. Tong. (2007). Crystal structure of the heterotrimer core of
Saccharomyces cerevisiae AMPK homologue SNF1. Nature, 449, 492-495. (*–equal first
authors)
120. J. Benach, L. Wang,* Y. Chen,* C.K. Ho, S. Lee, J. Seetharaman, R. Xiao, T.B. Acton, G.T.
Montelione, H. Deng, R. Sun$ & L. Tong.$ (2007). Structural and functional studies of the
abundant tegument protein ORF52 from murine gammaherpesvirus-68. J. Biol. Chem. 282,
31534-31541. (*–equal second authors, $–co-corresponding authors)
121. S. Xiang & L. Tong. (2008). Crystal structures of human and S. aureus pyruvate carboxylase and
molecular insights into the carboxyltransfer reaction. Nature Struct. Mol. Biol. 15, 295-302.
122. S. Metzger, J. Rong, H.-P. Nguyen, A. Cape, J. Tomiuk, A. Soehn, P. Propping, Y. FreudenbergHua, J. Freudenberg, L. Tong, S.-H. Li, X.-J. Li & O. Riess. (2008). Huntingtin-associated
protein-1 is a modifier of the age-at-onset of Huntington’s disease. Human Mol. Genet. 17,
1137-1146.
9
123. F. Forouhar, M. Abashidze, H. Xu, L.L. Grochowski, J. Seetharaman, M. Hussain, A. Kuzin, Y.
Chen, W. Zhou, R. Xiao, T.B. Acton, G.T. Montelione, A. Galinier, R.H. White & L. Tong.
(2008). Molecular insights into the biosynthesis of coenzyme F420. J. Biol. Chem. 283, 1183211840.
124. Y. Shen & L. Tong. (2008). Structural evidence for direct interactions between the BRCT
domains of human BRCA1 and a phospho-peptide from human ACC1. Biochem. 47, 5767-5773.
(Hot Article)
125. J.A. Vila, J.M. Aramini, P. Rossi, A. Kuzin, M. Su, J. Seetharaman, R. Xiao, L. Tong, G.T.
Montelione & H.A. Scheraga. (2008). Quantum chemical 13C chemical shift calculations for
protein NMR structure determination, refinement, and validation. Proc. Natl. Acad. Sci. USA,
105, 14389-14394.
126. S.J. Thackray, C. Bruckmann, J.L.R. Anderson, L.P. Campbell, R. Xiao, L. Zhao, C.G. Mowat, F.
Forouhar, L. Tong & S.K. Chapman. (2008). Histidine 55 of tryptophan 2,3-dioxygenase is not an
active site base but regulates catalysis by controlling substrate binding. Biochem. 47, 1067710684.
127. Y.J. Huang, D. Hang, L.J. Lu, L. Tong, M.B. Gerstein & G.T. Montelione. (2008). Targeting the
human cancer pathway protein interaction network by structural genomics. Mol. Cell.
Proteomics, 7, 2048-2060.
128. S.M. Vorobiev, M. Su, J. Seetharaman, Y.J. Huang, C.X. Chen, M. Maglaqui, H. Janjua, M.
Proudfoot, A. Yakunin, R. Xiao, T.B. Acton, G.T. Montelione & L. Tong. (2009). Crystal
structure of human retinoblastoma binding protein 9 (RBBP9). Proteins, 74, 526-529.
129. N. Zhao, J. Guan, F. Forouhar, T.J. Tschaplinski, Z.-M. Cheng, L. Tong & F. Chen. (2009). Two
poplar methyl salicylate esterases display comparable biochemical properties but divergent
expression patterns. Phytochem. 70, 37-44.
130. S.-W. Park, P.-P. Liu, F. Forouhar, A.C. Vlot, L. Tong, K. Tietjen & D.F. Klessig. (2009). Use of
a synthetic salicylic acid analog to investigate the roles of methyl salicylate and its esterases in
plant disease resistance. J. Biol. Chem. 284, 7307-7317.
131. Y.-S. Hsiao, D. Parker, A.J. Ratner, A. Prince & L. Tong. (2009). Crystal structures of respiratory
pathogen neuraminidases. Biochem. Biophys. Res. Commun. 380, 467-471.
132. C. Bertonati, M. Punta, M. Fischer, G. Yachdav, F. Forouhar, W. Zhou, A.P. Kuzin, J.
Seetharaman, M. Abashidze, T.A. Ramelot, M.A. Kennedy, J.R. Cort, A. Belachew, J.F. Hunt, L.
Tong, G.T. Montelione & B. Rost. (2009). Structural genomics reveals EVE as a new
ASCH/PUA-related domain. Proteins, 75, 760-773.
133. S. Xiang, A. Cooper-Morgan, X. Jiao, M. Kiledjian, J.L. Manley & L. Tong. (2009). Structure
and function of the 5’3’ exoribonuclease Rat1 and its activating partner Rai1. Nature, 458,
784-788.
134. C.-Y. Chou, L.P.C. Yu & L. Tong. (2009). Crystal structure of biotin carboxylase in complex
with substrates and implications for its catalytic mechanism. J. Biol. Chem. 284, 11690-11697.
135. L.P.C. Yu, S. Xiang, G. Lasso, D. Gil, M. Valle & L. Tong. (2009). A symmetrical tetramer for S.
aureus pyruvate carboxylase in complex with coenzyme A. Structure, in press
Reviews, book chapters, and invited papers
1.
A. M. de Vos, L. Tong, M. V. Milburn, P. M. Matias & S.-H. Kim. (1988). Plenum Publishing
Corporation, New York. Three-dimensional structure of ras p21 proteins. The guanine-nucleotide
binding proteins: common structural and functional properties, Proceedings of an EMBONATO-CEC Advanced Research Workshop 165, 27-34.
2.
S.-H. Kim, A. M. de Vos, L. Tong, M. V. Milburn, P. M. Matias, J. Jancarik & S. Nishimura.
(1988). ras Oncogene proteins: Three-dimensional structures, functional implications, and a
10
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
model for signal transducer. Cold Spring Harbor Symposia on Quantitative Biology, LIII, 273281.
M. G. Rossmann, R. McKenna, L. Tong, D. Xia, J.-B. Dai, H. Wu, H.-K. Choi, D. Marinescu &
R. E. Lynch. (1992). E. Dodson, S. Gover, and W. Wolf, eds. Science and Engineering Research
Council, Daresbury. Molecular replacement real space averaging. Proc. of the CCP4 Study
Weekend, 31 January - 1 February, 1992, 33-48.
L. Tong & M. G. Rossmann. (1997). Rotation function calculations with GLRF program.
Methods in Enzymology, 276, 594-611.
H. Wu, Y. C. Park, H. Ye & L. Tong (1999). Structural studies of human TRAF-2. Cold Spring
Harbor Symp. Quant. Biol. (Signaling and gene expression in the immune system). 64, 541-549.
Z. Yang & L. Tong. (2000). Structural studies of a human malic enzyme. Protein and Peptide
Lett. 7, 287-296.
L. Tong. (2001). Translation functions. Chapter 13.3 of International Tables for
Crystallography, Vol. F. Crystallography of Biological Macromolecules. M. G. Rossmann & E.
Arnold Eds. Kluwer Academic Publishers, The Netherlands.
L. Tong. (2001). How to take advantage of non-crystallographic symmetry in molecular
replacement: The locked rotation and translation functions. Acta Cryst. D57, 1383-1389.
R. Batra, R. Khayat & L. Tong. (2001). Structural studies of herpesvirus proteases. Protein and
Peptide Lett. 8, 333-342.
L. Tong. (2002). Viral proteases. Chemical Reviews, 102, 4609-4626.
G.-G. Chang & L. Tong. (2003). Structure and function of malic enzymes, a new class of
oxidative decarboxylases. Biochem. 42, 12721-12733.
G. Jogl, Y.-S. Hsiao & L. Tong. (2004). Structure and function of carnitine acyltransferases. Ann.
N.Y. Acad. Sci. 1033, 17-29.
L. Tong. (2005). Toll/interleukin-1 receptors and innate immunity. in G. Waksman, M.G.
Caparon & S. Hultgren, eds. Structural basis of bacterial pathogenesis. pp. 241-263. ASM
Press, Washington, DC.
L. Tong. (2005). Acetyl-coenzyme A carboxylase: Crucial metabolic enzyme and attractive target
for drug discovery. Cell. Mol. Life Sci. 62, 1784-1803.
L. Tong & H.J. Harwood Jr. (2006). Acetyl-coenzyme A carboxylases: versatile targets for drug
discovery. J. Cell. Biochem. 99, 1476-1488
J.A. Khan, F. Forouhar, X. Tao & L. Tong. (2007). Nicotinamide adenine dinucleotide
metabolism as attractive target for drug discovery. Expert Opin. Thera. Targets, 11, 695-705.
C.R. Mandel & L. Tong. (2007). How to get all “A”s in polyadenylation. Structure, 15, 10241026.
F. Forouhar, A. Kuzin, J. Seetharaman, I. Lee, W. Zhou, M. Abashidze, Y. Chen, W. Yong, H.
Janjua, Y. Fang, D. Wang, K. Cunningham, R. Xiao, T.B. Acton, E. Pichersky, D.F. Klessig,
C.W. Porter, G.T. Montelione, & L. Tong. (2007). Functional insights from structural genomics.
J. Struct. Funct. Genomics, 8, 37-44.
D.F. Klessig, D. Kumar, F. Forouhar, L. Tong, Y. Yang, E. Pichersky, G.T. Montelione, Y. Chen,
S.W. Park, A.C. Vlot & V. Shulaev. (2007). Salicylic acid-binding protein (SABP) 2 and
systemic acquired resistance. In F. Sanchez, C. Quinto, I. M. Lopez-Lara & O. Geiger, eds.
Biology of Plant-Microbe Interactions, 5, 176-181.
11
20.
21.
22.
23.
24.
Structural Genomics Consortium, China Structural Genomics Consortium, Northeast Structural
Genomics Consortium, S. Gräslund, P. Nordlund, J. Weigelt, J. Bray, O. Gileadi, S. Knapp, U.
Oppermann, C. Arrowsmith, R. Hui, J. Ming, S. dhe-Paganon, H.W. Park, A. Savchenko, A. Yee,
A. Edwards, R. Vincentelli, C. Cambillau, R. Kim, S.H. Kim, Z. Rao, Y. Shi, T.C. Terwilliger,
C.Y. Kim, L.W. Hung, G.S. Waldo, Y. Peleg, S. Albeck, T. Unger, O. Dym, J. Prilusky, J.L.
Sussman, R.C. Stevens, S.A. Lesley, I.A. Wilson, A. Joachimiak, F. Collart, I. Dementieva, M.I.
Donnelly, W.H. Eschenfeldt, Y. Kim, L. Stols, R. Wu, M. Zhou, S.K. Burley, J.S. Emtage, J.M.
Sauder, D. Thompson, K. Bain, J. Luz, T. Gheyi, F. Zhang, S. Atwell, S.C. Almo, J.B. Bonanno,
A. Fiser, S. Swaminathan, F.W. Studier, M.R. Chance, A. Sali, T.B. Acton, R. Xiao, L. Zhao,
L.C. Ma, J.F. Hunt, L. Tong, K. Cunningham, M. Inouye, S. Anderson, H. Janjua, R. Shastry,
C.K. Ho, D. Wang, H. Wang, M. Jiang, G.T. Montelione, D.I. Stuart, R.J. Owens, S. Daenke, A.
Schütz, U. Heinemann, S. Yokoyama, K. Büssow & K.C. Gunsalus. (2008). Protein production
and purification. Nature Methods, 5, 135-146.
C.R. Mandel, Y. Bai & L. Tong. (2008). Protein factors in pre-mRNA 3’-end processing. Cell.
Mol. Life Sci. 65, 1099-1122.
L. Tong, E. Arnold & M.G. Rossmann. (2008). Patterson and molecular-replacement techniques.
Chapter 2.3 of International Tables for Crystallography, Vol. B. U. Shmueli Ed. Kluwer
Academic Publishers, The Netherlands.
S.J. Thackray, C. Bruckmann, C.G. Mowat, F. Forouhar, S.K. Chapman & L. Tong. (2008).
Indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase. Handbook of Metalloproteins. A.
Messerschmidt Ed., John Wiley & Sons, Chichester, UK.
X. Tao & L. Tong. (2009). Expression, purification and crystallization of Toll/Interleukin-1
Receptor (TIR) Domains. Methods Mol. Biol., Vol. 517, Chapter 6, pp. 81-88. C.E. McCoy &
L.A.J. O’Neill Ed., Humana Press.
12