Test sample - enzymepreparation from various bacterial sources.
Ethylenediaminetetra-acetic acid sodiumsalt.(EDTA)
0.1M EDTA,pH 7
Hazardous Substances Policy - Assessment
Sodium phosphate
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
Dr.T.W.Young
Assessor
A.Wadeson
Assessment Number*
1682
Date of Assessment
16/11/00
Signature
Signature
Notes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
G6
List names where possible
Dr T,W.Young,A.Wadeson and all other members of Dr T.W.Young's research
team. Final year project students.
3
ACTIVITY ASSESSED
4
MATERIALS INVOLVED
NAME
Inorganic pyrophosphatase assay.
AMOUNT**
Attach copies of data sheet(s)
HAZARD
Test sample - enzyme
preparation from
various bacterial sources.
Variable
usually in
the range
20mls to
200mls
Ethylenediaminetetraacetic acid sodium
salt.(EDTA)
500g
HARMFUL.
0.1M EDTA,pH 7
3.72g/100
mls
N. S. H.
Sodium phosphate
IRRITANT
Sodium Chloride
500g
N. S. H.
CHES
100g
IRRITANT
CAPS
25g
RISK PHRASES
HAZDAT NO***
NO RISK PHRASES
AVAILABLE.
Avoid inhalation,skin and
eye contact.
May be possible biological
hazard see assessment
1683/G33
N/A
R22- Harmful if swallowed.
R36/37/38- Irritating to
eyes,respiratory system
and skin.
1252
N/A
R36/37/38- Irritating to
eyes,respiratory system
and skin.
693
1303
R36/37/38- Irritating to
eyes,respiratory system
and skin.
SIGMA
AVOID CONTACT.
TOXICOLOGICAL
PROPERTIES NOT
THROUGHLY
INVESTIGATED.
SIGMA
BIOSCIENCESNO***
TAPS FREE ACID
25g
AVOID CONTACT.
TOXICOLOGICAL
PROPERTIES NOT
THROUGHLY
INVESTIGATED.
SIGMA
TES FREE ACID
100g
AVOID CONTACT.
TOXICOLOGICAL
PROPERTIES NOT
THROUGHLY
INVESTIGATED.
SIGMA
DL-Serine
25g
N. S. H.
Sodium pyrophosphate
100g
IRRITANT
Magnesium chloride
500g
N. S. H.
Sodium hydroxide
500g
CORROSIVE
R35- Causes severe
burns.
302
1M Sodium hydroxide
20mls
CORROSIVE
R34- Causes burns.
SEE302
Sodium dihydrogen
phosphate
500g
HARMFUL
IRRITANT
R36/37/38- Irritating to
eyes,respiratory system
and skin.
641
0.4mM and 4mM Sodium
dihydrogen phosphate
0.0552g/
litre
N.S.H.
Ammonium molybdate
100g
HARMFUL
R22- Harmful if swallowed.
FISHER
Ammonium molybdate
solution - 2.5%w/v
Ammonium molybdate in
2.5M Sulphuric acid.
100mls
CORROSIVE
R35- Causes severe
burns.
N/A
Sulphuric acid
2.5 litre
CORROSIVE
R35- Causes severe
burns.
431
2.5M Sulphuric acid
100mls
CORROSIVE
R35- Causes severe
burns.
431
1-Amino-2-napthol-4sulphonic acid.(ANSA)
25g
CORROSIVE,
HARMFUL
R34- Causes burns
R22- Harmful if swallowed.
SIGMA
Sodium sulphite
100g
HARMFUL
R22- Harmful if swallowed.
R36/38- Irritating to eyes
and skin.
R40- Possible risk of
irreversible effects.
4454
FISHER
Sodium metabisulphite.
500g
HARMFUL,
IRRITANT.
R20/21/22- Harmful by
inhalation in contact with
skin and if swallowed.
R31- Contact with acids
liberates toxic gas.
R37- Irritating to
respiratory system.
R41- Risk of serious
damage to eyes.
SIGMA
1410
SIGMA
R36/37/38- Irritating to
eyes,respiratory system
and skin
SIGMA
1156
1210
SEE641
Substrate solution-A
50mM DL Serine +(OR
CHES/CAPS/TES/TAPS/
SODIUM CHLORIDE)
2mM Sodium
pyrophosphate +
2mM EDTA.(pH 9)
100mls
N. S. H.
N/A
Substrate solution-B
10mM Magnesium
chloride.(pH 9)
100mls
N. S. H.
N/A
Fiske-Subbarow
reagent.9ml Distilled
water + 2.5ml Ammonium
molybdate reagent + 1ml
ANSA reagent.
Variable
usually
12.5mls
IRRITANT.
R36/37/38- Irritating to
eyes,respiratory system
and skin.
N/A
ANSA - reagent
0.25%ANSA +
13.3% Sodiun
metabisulphite +
0.5% Sodium sulphite.
100mls
IRRITANT.
R36/37/38- Irritating to
eyes,respiratory system
and skin
N/A
Cobalt Nitrate
500g
HARMFUL,
OXIDISING,
SENSITISER
R8- Contact with
combustible material may
cause fire.
R22- Harmful if swallowed.
R36/37/38- Irritating to
eyes,respiratory system
and skin.
R43- May cause
sensitisation by skin
contact.
SIGMA
100mM Cobalt Nitrate
2.91g/100
mls.
HARMFUL,
OXIDISING,
SENSITISER
R8- Contact with
combustible material may
cause fire.
R22- Harmful if swallowed.
R36/37/38- Irritating to
eyes,respiratory system
and skin.
R43- May cause
sensitisation by skin
contact.
N/A
Zinc Sulphate
500g
IRRITANT.
DANGEROUS TO THE
ENVIRONMENT.
R36/38- Irritating to eyes
and skin.
R50/53- Very toxic to
aquatic organisms, may
cause long teerm adverse
effects in the aquatic
enviroment.
1142
SIGMA
100mM Zinc Sulphate
2.875g/
100mls
IRRITANT.
DANGEROUS TO THE
ENVIRONMENT.
R36/38- Irritating to eyes
and skin.
R50/53- Very toxic to
aquatic organisms, may
cause long teerm adverse
effects in the aquatic
enviroment.
N/A
Calcium Chloride
500g
IRRITANT.
R36- Irritating to eyes.
1312
100mM Calcium Chloride
1.11g/100
mls
IRRITANT.
R36/37/38- Irritating to
eyes,respiratory system
and skin
N/A
Manganese chloride
100g
HARMFUL
R22- Harmful if swallowed.
R36/37/38- Irritating to
eyes,respiratory system
and skin.
R40- Possible risk of
irreversible effects.
1337
SIGMA
100mM Manganese
chloride
1.979g/
100mls
HARMFUL
R22- Harmful if swallowed.
R36/37/38- Irritating to
eyes,respiratory system
and skin.
R40- Possible risk of
irreversible effects.
N/A
5
INTENDED USE
Give brief details and attach protocol/instructions
For the assay of inorganic pyrophosphatase in bacterial cell extracts,
and the addition of various metal salts to the assay which affect the activity of inorganic pyrophosphatase.
6
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
Possible microbiological risk from enzyme preparations containing viable cells.
Risk of irreversible effects from handling Harmful Sodium sulphite and Manganese Chloride in solid and liquid
form; avoid dusting / inhalation, aerosol formation during transfer operations and skin contact.
Risk of eye,skin and clothing contact when making assay solutions from corrosive, harmful and irritant chemicals
and chemicals where the hazards are not fully investigated and especially when diluting concentrated sulphuric
acid, (see assessment 1584/433) and sodium hydroxide for pH adjustment..
7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
The risks in section 6 above are minimised by using Good Chemical and Microbiological Practice together with
the precautions laid down in section 8.
University carcinogen guidelines USP/80/CR/2 should be followed for substances with R40.
Metal salts should be treated and handled with great care avoiding inhalation and skin contact.
8
CONTROL MEASURES
Additional to Good Chemical Practice
Good microbiological practice to containment level 1 should be used when
handling bacterial enzyme extracts, all labware in contact with enzyme extracts should be autoclaved.
Sulphuric acid should only be dispensed in the fume cupboard and a winchester carrier should be used for
transportation of stock bottles.(see assessment 1584/433). Sodium hydroxide used for pH adjustment need only
be made in small volumes, for example in 20ml and should be dispensed with care. Dusting of reagents on
weighing should be avoided, sodium sulphite, manganese chloride and cobalt nitrate to be weighed in safety
cabinet in G3. University carcinogen guidlines USP/80/CR/2 should be followed for substances with (R40)
Fiske-Subbarow reagent when prepared should be kept capped along with assay tubes( microfuge tubes
can be used for the assay.)Gloves and safety glassess should be worn throughout the procedure.
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
Check integrity of gloves.
Check safety glasses are in good order and clean.
Check fume cupboard is working before use.
10
MONITORING
Performance of control measures,
Personal exposure
Health Surveillance
11
WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
All possible microbiological waste from contact with the enzyme solution should be autoclaved before disposal.
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
14/11/05 or
Any change in the protocol.
13
EMERGENCY ACTION
TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Spills of solid compounds should be contained using the lab chemical spill kit. The contained waste should go to
the School special waste coordinator who will advise on safe disposal. Data sheets for sodium
hydroxide(Unicoshh 302),cobalt nitrate, manganese chloride, calcium chloride,zinc sulphate,
1- Amino-2-napthol-4-sulphonic acid, Sodium metabisulfite, DL-Serine, Sodium pyrophosphate, Sodium sulphite,
Ammonium molybdate, Sodium dihydrogen phosphate, Magnesium chloride, Sodium phosphate ,
Ethylenediaminetetra-acetic acid, Sodium chloride, TES free acid, TAPS free acid, CAPS and CHES
are attached, these give more information on spillages.
Spills of concentrated and dilute sulphuric acid,use sodium bicarbonate and contain spill in a suitable corrosion
resistant container and label. The contained waste should go to the School special waste coordinator who will
advise on safe disposal.See attached Unicoshh hazard data sheet number 431.Note neutralising acid spills
will produce large amounts of carbon dioxide gas, ensure adequate ventilation.
Evacuation, protection for personnel involved in clean-up, Special First Aid
TO PROTECT PERSONNEL
See data sheeets attached for details of the chemical in question.
ONLY PERSONNEL WHO ARE ADEQUATELY TRAINED SHOULD ATTEMPT TO NEUTRALISE/CLEAR UP
SPILLAGES.
Clean-up/decontamination
TO RENDER SITE OF EMERGENCY SAFE
After cleaning up spill,wash site with soap and water.
CONTACT
A.Wadeson or First aider.
PHONE
3523 or see
first aider list
10.10.00
*
Prefix T is used for Teaching Assessment Number.
**
List the amount by weight of the substance used. (for liquids eg; 100 mls 1M Sodium Hydroxide =
4g).
***
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.