The Stages of Mitosis Prophase 1st stage of mitosis Chromatin form into chromosomes The nuclear envelope begins to break down The 2 pair of centrioles begin to move to opposite ends of the cell Spindle fibers and asters begin to appear – made of microtubules Metaphase 2nd stage of mitosis Chromosomes begin to line up at the middle (the equator) of the cell on specialized spindle fibers called kinetochore fibers Anaphase 3rd phase of mitosis The chromatids divide at the centromeres and move to opposite sides (poles) of the cell with the aid of specialized spindle fibers called polar fibers and kinetochore fibers Telophase The final phase of mitosis Centrioles and spindle fibers disappear Chromosomes become chromatin again Nuclear envelopes begin to form around the new developing daughter cells Cytokinesis Immediately follows mitosis (sometimes included in telophase) Occurs when the cytoplasm from the parent cell divides equally between the 2 new daughter cells o All organelles and structures are also divided equally during this process o Cell membrane pinches in and divides the cell at its center – called a cleavage furrow o Cell membrane eventually divides in two, forming 2 complete daughter cells – each with its own cell membrane