Figure 1. 14q23.1 deletion identified by Affy SNP array and UCSC Genome Browser image.
Scatter plots of the blood DNA (A) and myocardium DNA (B) by gray and purple points
respectively, representing the log2 ratio value of the region. The baseline in A and B was marked
as normal copy number while the spots above indicate amplification and the spots below
indicate deletion. Visualized copy number was generated from the mean of many probe signals of
blood DNA (C) and myocardium DNA (D). Corresponding physical position of deletion at
14q23.1 (E). The region contains the DAAM1 and KIAA0666 genes (from the UCSC Genome
Browser, March 2006).
Figure 2A. Quantification of DAAM1 abundance between the CHD fetal blood and control blood.
With three pairs of primers targeting different regions of DAAM1and normalized to β-actin, the
abundance in the three genomic regions in the CHD fetal blood was significantly lower than in the
control (P<0.001). Error bars represent the standard deviation (n≥3).
Figure 2B. Quantification of DAAM1 abundance between the CHD fetal myocardium and control
blood. DAAM1 abundance in the three genomic regions in the patient myocardium was
significantly lower than in the control blood (P<0.01). Error bars represent the standard deviation
(n≥3).